当前位置： SCI文献检索 > MOLECULAR AND CELLULAR BIOCHEMISTRY期刊下所有文献 > Expression of Sp7 in Satb2-induced osteogenic differentiation of mouse bone marrow stromal cells is regulated by microRNA-27a.

Expression of Sp7 in Satb2-induced osteogenic differentiation of mouse bone marrow stromal cells is regulated by microRNA-27a.

Abstract：

:Satb2 is a special AT-rich binding transcription factor essential for osteoblast differentiation and bone formation. Specific microRNAs (miRNAs) have been identified to regulate the complex process of osteogenic differentiation. It remains unclear how miRNA expressions is changed in the Satb2-induced osteogenic differentiation of bone marrow stromal cells (BMSCs). From the miRNA expression profile data collected by us from Satb2-induced osteogenic differentiation of mouse BMSCs, we found that miR-27a was significantly down-regulated relative to non-treated cells 7 days post induction. By in silico analysis, we identified Sp7 as a miR-27a targeting gene and verified the findings by Western blot analysis, qRT-PCR, and luciferase reporter assays. We also analyzed the function of miR-27a in osteogenic differentiation by transfection of exogenous miR-27a into BMSCs. Overexpression of miR-27a remarkably inhibited the expression of Sp7 and attenuated Satb2-induced osteogenic differentiation. Our results suggest that expression of Sp7 during the early stage of Satb2-induced osteogenic differentiation of BMSCs is regulated by miR-27a.

journal_name

Mol Cell Biochem

journal_title

Molecular and cellular biochemistry

authors

Gong Y,Lu J,Yu X,Yu Y

doi

10.1007/s11010-016-2709-y

subject

Has Abstract

pub_date

2016-06-01 00:00:00

pages

7-16

issue

1-2

eissn

0300-8177

issn

1573-4919

pii

10.1007/s11010-016-2709-y

journal_volume

417

pub_type

杂志文章

相关文献

MOLECULAR AND CELLULAR BIOCHEMISTRY文献大全
  • Transcriptional regulation of plasminogen activator inhibitor-1 in vascular endothelial cells induced by oxidized very low density lipoproteins.

    abstract：:Elevated levels of triglycerides and very low density lipoproteins (VLDL) are biochemical markers of metabolic syndrome and diabetes. VLDL from hypertriglyceridemic or diabetic patients increased the generation of plasminogen activator inhibitor-1 (PAI-1) from cultured vascular endothelial cells (EC). Susceptibility o...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/s11010-008-9851-0

    authors： Zhao R,Ma X,Shen GX

    更新日期：2008-10-01 00:00:00

  • Human tau protein forms complex with PrP and some GSS- and fCJD-related PrP mutants possess stronger binding activities with tau in vitro.

    abstract：:Microtubule associated protein tau is considered to play roles in some types of human transmissible spongiform encephalopathies (TSE). In this study, the full-length and several truncated human tau proteins were expressed from E. coli and purified. Using GST pull down, co-immunoprecipitation assay and tau-coated ELISA...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/s11010-007-9664-6

    authors： Wang XF,Dong CF,Zhang J,Wan YZ,Li F,Huang YX,Han L,Shan B,Gao C,Han J,Dong XP

    更新日期：2008-03-01 00:00:00

  • Pyruvate modulates antioxidant status of cultured human lens epithelial cells under hypergalactosemic conditions.

    abstract：:Lens epithelial cells are the metabolic unit of the lens and antioxidant enzymes are mainly concentrated here. The purpose of this study was to maintain human lens epithelial cells (HLEC) in culture and examine the status of antioxidant enzymes (glutathione peroxidase (GSHPx), catalase (CAT), glutathione-S-transferase...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1023/a:1019961922260

    authors： Mohanty I,Joshi S,Trivedi D,Srivastava S,Tandon R,Gupta SK

    更新日期：2002-09-01 00:00:00

  • Alterations of biochemical marker levels and myonuclear numbers in rat skeletal muscle after ischemia-reperfusion.

    abstract：:Prolonged ischemia-reperfusion results in various damages in skeletal muscle. Following reperfusion, although the damaged muscles undergo regeneration, the precise process and mechanism of regeneration have not yet been fully understood. Here, we show the altered levels of plasma biochemical markers of muscle damage, ...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/s11010-012-1470-0

    authors： Itoh M,Shimokawa N,Tajika Y,Murakami T,Aotsuka N,Lesmana R,Farenia R,Iwasaki T,Okda J,Yorifuji H,Koibuchi N

    更新日期：2013-01-01 00:00:00

  • Effect of chronic colchicine administration on the myocardium of the aging spontaneously hypertensive rat.

    abstract：:Colchicine has been demonstrated to suppress the release of fibroblast growth factors, retard collagen formation and augment collagenase activity. Trials with colchicine in patients with hepatic fibrosis have suggested clinical benefit. The development of impaired myocardial function in the spontaneously hypertensive ...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1023/a:1006889126666

    authors： Cicogna AC,Brooks WW,Hayes JA,Robinson KG,Sen S,Conrad CH,Bing OH

    更新日期：1997-01-01 00:00:00

  • Respiratory muscle injury in animal models and humans.

    abstract：:Respiratory muscle injury may result from excessive loading due to a decrease in respiratory muscle strength, an increase in the work of breathing, or an increase in the rate of ventilation. Other conditions such as hypoxemia, hypercapnia, aging, decreased nutrition, and immobilization may potentiate respiratory muscl...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章,评审

    doi：10.1023/a:1006803703128

    authors： Reid WD,MacGowan NA

    更新日期：1998-02-01 00:00:00

  • FT-IR spectroscopy of methylmercury-exposed mouse lung.

    abstract：:Infrared spectroscopy which has traditionally been utilized by chemists and physicists for characterization and identification of the structural properties of chemical compounds is now becoming more relevant as a biodiagnostic tool. Recent reports suggest that arthritis and Alzheimer's disease can be diagnosed by usin...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/BF00925716

    authors： Das RM,Ahmed MK,Mantsch HH,Scott JE

    更新日期：1995-04-12 00:00:00

  • Expression of the ErbB4 receptor causes reversal regulation of PP2A in the Shc signal transduction pathway in human cancer cells.

    abstract：:Expression of ErbB4 receptor is correlated with the incidence of non-metastatic types of human cancers, whereas the overexpression of other ErbB receptor families (ErbB1/EGFR, ErbB2 and ErbB3) is correlated to the formation of metastatic tumors. However, the molecular mechanism underlying this phenomenon has been uncl...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/s11010-005-9075-5

    authors： Yumoto N,Yu X,Hatakeyama M

    更新日期：2006-04-01 00:00:00

  • Modulation of antioxidant enzyme activities, platelet aggregation and serum prostaglandins in rats fed spray-dried milk containing n-3 fatty acid.

    abstract：:Spray-dried milk enriched with n-3 fatty acids from linseed oil (LSO) or fish oil (FO) were fed to rats to study its influence on liver lipid peroxides, hepatic antioxidant enzyme activities, serum prostaglandins and platelet aggregation. Significant level of alpha linolenic acid, eicosapentaenoic acid and docosahexae...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/s11010-005-7094-x

    authors： Ramaprasad TR,Baskaran V,Krishnakantha TP,Lokesh BR

    更新日期：2005-09-01 00:00:00

  • Inhibition of Helicobacter pylori in vitro by various berry extracts, with enhanced susceptibility to clarithromycin.

    abstract：:The objective of this study was to evaluate the effects of various berry extracts, with and without clarithromycin on Helicobacter pylori. Resistance to clarithromycin by H. pylori has been reported, leading to interest in alternatives/adjuncts to therapy with clarithromycin. H. pylori American type culture collection...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1023/b:mcbi.0000044310.92444.ec

    authors： Chatterjee A,Yasmin T,Bagchi D,Stohs SJ

    更新日期：2004-10-01 00:00:00

  • Angiotensin converting enzyme inhibition in Dahl salt-sensitive rats.

    abstract：:Angiotensin II has previously been reported to have in vivo and in vitro cardiac hypertrophic effects. We used the salt-sensitive Dahl rat genetic strain to separate mechanical (pressure overload) vs. hormonal (renin-angiotensin system) input in cardiac hypertrophy. Blood pressure was significantly increased and left ...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/BF00229802

    authors： Peeler TC,Baker KM,Esmurdoc CF,Chernin MI

    更新日期：1991-05-29 00:00:00

  • An inverse relation between mitochondrial hexokinase content and phosphoglucomutase activity of rat tissues.

    abstract：:The hexokinase: fumarase ratios of mitochondria isolated from ten tissues of the rat were determined, and compared with the tissue content of phosphoglucomutase and phosphorylase, taken as representatives of enzymes concerned with glycogen metabolism. A generally inverse relationship was found between the mitochondria...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/BF00215278

    authors： Wilson JE,Felgner PL

    更新日期：1977-11-25 00:00:00

  • Deferiprone (L1) induced conformation change of hemoglobin: A fluorescence and CD spectroscopic study.

    abstract：:The interaction of deferiprone (1,2-dimethyl-3-hydroxy-pyrid-4-one) L1, the first clinically available oral iron chelator, with the tetrameric allosteric protein hemoglobin from human red blood cells has been investigated spectrofluorometrically and by circular dichroism spectroscopy. The interaction is hydrogenbond l...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1023/a:1007049701572

    authors： Chakraborty D,Bhattacharyya M

    更新日期：2000-01-01 00:00:00

  • Inhibition of hepatic stellate cell proliferation by heat shock protein 90 inhibitors in vitro.

    abstract：:Hepatic stellate cells (HSCs) play an important role in the development of hepatic fibrosis. Heat shock protein 90 (Hsp90) is essential for the maturation and activity of a varied group of proteins involved in signal transduction and cell cycle regulation. In this study, we found that two Hsp90 inhibitors, VER-49009 a...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/s11010-009-0131-4

    authors： Sun X,Zhang XD,Cheng G,Hu YH,Wang HY

    更新日期：2009-10-01 00:00:00

  • Effect of nifedipine administration on the functional capacity of neutrophils.

    abstract：:Administration of nifedipine to mice over a period of six months caused a significant (p < 0.05) decrease in neutrophilic functions viz superoxide generation, coupled to NADPH oxidase activity as well as NADPH production by HMP shunt. Properties like chemotaxis and phagocytosis showed a similar decrease. From this stu...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/BF00925987

    authors： Kaul N,Andrabi KI,Ganguly NK,Wahi PL

    更新日期：1993-03-10 00:00:00

  • Modulation of fatty acid-binding capacity of heart fatty acid-binding protein by oxygen-derived free radicals.

    abstract：:In this study, we examined the effects of exposure of heart fatty acid-binding protein (h-FABP) to chemically generated O2- or OH. with respect to its oleate binding and to its electrophoretic properties. Purified rat h-FABP at 40 microM scavenged as much as 30% O2- and 85% OH.. On the other hand, when 2 nmol (4 micro...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/BF00231380

    authors： Jones RM,Prasad MR,Das DK

    更新日期：1990-10-15 00:00:00

  • Activation of peroxisome proliferator-activated receptors alpha and gamma1 inhibits human smooth muscle cell proliferation.

    abstract：:Atherosclerotic lesions occur as a result of excess lipid deposition within the vascular tissues. The peroxisome proliferator-activated receptors (PPARs) present in adipose and hepatic tissues have been shown to promote fatty acid oxidation and lipid storage. An immunohistochemical assessment of PPARalpha and PPARgamm...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：

    authors： Zahradka P,Yurkova N,Litchie B,Moon MC,Del Rizzo DF,Taylor CG

    更新日期：2003-04-01 00:00:00

  • In vitro cellular uptake and neuroprotective efficacy of poly-arginine-18 (R18) and poly-ornithine-18 (O18) peptides: critical role of arginine guanidinium head groups for neuroprotection.

    abstract：:We have previously demonstrated that Cationic Arginine-Rich Peptides (CARPs) and in particular poly-arginine-18 (R18; 18-mer of arginine) exhibit potent neuroprotective properties in both in vitro and in vivo neuronal injury models. Based on the current literature, there is a consensus that arginine residues by virtue...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/s11010-019-03646-0

    authors： MacDougall G,Anderton RS,Ouliel E,Gao J,Redmond SL,Knuckey NW,Meloni BP

    更新日期：2020-01-01 00:00:00

  • Identification of a novel intergenic miRNA located between the human DDC and COBL genes with a potential function in cell cycle arrest.

    abstract：:Frequent abnormalities in 7p12 locus in different tumors like lung cancer candidate this region for novel regulatory elements. MiRNAs as novel regulatory elements encoded within the human genome are potentially oncomiRs or miR suppressors. Here, we have used bioinformatics tools to search for the novel miRNAs embedded...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/s11010-017-3242-3

    authors： Hoballa MH,Soltani BM,Mowla SJ,Sheikhpour M,Kay M

    更新日期：2018-07-01 00:00:00

  • Role of hyaluronan and CD44 in reactive oxygen species-induced mucus hypersecretion.

    abstract：:Mucus hypersecretion is an important manifestation in patients with chronic inflammatory airway diseases. Mucin 5AC (MUC5AC) is a major component of airway mucus. MUC5AC expression is regulated by epidermal growth factor receptor (EGFR) which can be activated by reactive oxygen species (ROS). Hyaluronan (HA), a linear...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/s11010-011-0740-6

    authors： Yu H,Li Q,Zhou X,Kolosov VP,Perelman JM

    更新日期：2011-06-01 00:00:00

  • Metabolic adaptation of the renal carbohydrate metabolism. III. Effects of high protein diet on the gluconeogenic and glycolytic fluxes in the proximal and distal renal tubules.

    abstract：:The adaptive response of renal metabolism of glucose was studied in isolated rat proximal and distal renal tubules after a high protein-low carbohydrate diet administration. This nutritional situation significantly stimulated the gluconeogenic activity in the renal proximal tubules (about 1.5 fold at 48 hours) due, in...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/BF00221209

    authors： García-Salguero L,Lupiáñez JA

    更新日期：1989-10-31 00:00:00

  • Phosphoglucoisomerase-catalyzed interconversion of hexose phosphates. Study by 13C NMR of proton and deuteron exchange.

    abstract：:The exchange of protons and deuterons by phosphoglucoisomerase during the single passage conversion of D-[2-13C,1-2H]fructose 6-phosphate in H2O or D-[2-13C]fructose 6-phosphate in D2O to D-[2-13C]glucose 6-phosphate, as coupled with the further generation of 6-phospho-D-[2-13C]gluconate in the presence of excess gluc...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/BF00227479

    authors： Malaisse WJ,Liemans V,Malaisse-Lagae F,Ottinger R,Willem R

    更新日期：1991-05-15 00:00:00

  • Expression of stress proteins (HSP-70 and HSP-90) in the rabbit urinary bladder subjected to partial outlet obstruction.

    abstract：:Partial obstruction of the rabbit bladder outlet induces a rapid hypertrophy characterized by increased bladder mass, increased smooth muscle content, and increased collagen deposition. In addition, partial outlet obstruction induces decreased contractile responses to both field stimulation and postsynaptic receptor s...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/BF01084267

    authors： Zhao Y,Chacko S,Levin RM

    更新日期：1994-01-12 00:00:00

  • Current ideas on the significance of protein glycosylation.

    abstract：:Carbohydrate has been removed from a number of glycoproteins without major effect on the structure or enzyme activity of the protein. Thus carbohydrate has been suggested to underly a non-primary function for proteins, such as in relatively non-specific interactions with other carbohydrates or macromolecules, stabiliz...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章,评审

    doi：10.1007/BF00230632

    authors： West CM

    更新日期：1986-11-01 00:00:00

  • p38 Mitogen-activated protein kinase affects transforming growth factor-beta/Smad signaling in human dental pulp cells.

    abstract：:Transforming Growth Factor-beta (TGF-beta) plays an essential role in differentiation of dental pulp cells into odontoblasts during reparative dentine formation. However, the mechanism by which TGF-beta stimulates dental repair remains rather obscure. Human dental pulp cells were used as an in vitro model in the prese...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/s11010-006-9193-8

    authors： Wang FM,Hu T,Tan H,Zhou XD

    更新日期：2006-10-01 00:00:00

  • Nuclear factor-κB signaling pathway is involved in phospholipase Cε-regulated proliferation in human renal cell carcinoma cells.

    abstract：:Phospholipase Cε (PLCε), a downstream effector of small GTPase superfamily, has been identified to play a crucial role in tumorigenesis. Previously, our studies have showed that PLCε promotes proliferation of renal cell carcinoma (RCC) cells. However, the molecular mechanisms by which PLCε enhances the survival phenot...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/s11010-013-1948-4

    authors： Du HF,Ou LP,Song XD,Fan YR,Yang X,Tan B,Quan Z,Luo CL,Wu XH

    更新日期：2014-04-01 00:00:00

  • Methionine and methionine sulfoxide alter parameters of oxidative stress in the liver of young rats: in vitro and in vivo studies.

    abstract：:It has been shown that elevation of plasma methionine (Met) and its metabolites may occur in several genetic abnormalities. In this study we investigated the in vitro and in vivo effects of the Met and methionine sulfoxide (MetO) on oxidative stress parameters in the liver of rats. For in vitro studies, liver homogena...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/s11010-013-1777-5

    authors： Costa MZ,da Silva TM,Flores NP,Schmitz F,da Silva Scherer EB,Viau CM,Saffi J,Barschak AG,de Souza Wyse AT,Spanevello RM,Stefanello FM

    更新日期：2013-12-01 00:00:00

  • L-tyrosine: 2-oxoglutarate aminotransferase induction by hydrocortisone in the thymus of the white rat.

    abstract：:Hydrocortisone hemisuccinate within 4 hours after in vivo administration produced an increase in precursor incoporation into rat thymus RNA and proteins in the whole animal. From these results, together with information obtained from measurements of the tyrosine aminotransferase activity and the action of mitomycin C ...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/BF01751307

    authors： Abraham AD

    更新日期：1975-12-31 00:00:00

  • Osteopontin and cardiovascular system.

    abstract：:A matricellular protein, osteopontin (OPN), is expressed in response to mechanical stress and similar stimuli in the heart, integrates the inter-ECM signal transduction network of component cells, and maintains efficient contractility through quantitative and qualitative control of extracellular matrix (ECM) proteins....

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章,评审

    doi：10.1007/s11010-006-9368-3

    authors： Okamoto H

    更新日期：2007-06-01 00:00:00

  • Isolation and characterization of a tRNA(guanine-7-)-methyltransferase from Salmonella typhimurium.

    abstract：:The tRNA modifying enzyme, S-adenosylmethionine:tRNA(guanine-7-)-methyltransferase, has been extensively purified from Salmonella typhimurium. A rapid and efficient purification method using phosphocellulose chromatography followed by ammonium sulfate precipitation and Sephadex G-100 gel filtration is described. The e...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/BF00224919

    authors： Colonna A,Ciliberto G,Santamaria R,Cimino F,Salvatore F

    更新日期：1983-01-01 00:00:00