Abstract：
:The ability of 17 monoclonal antibodies (MoAb) against measles virus haemagglutinin (MV-H) to bind to 10 selected MV-H-specific synthetic peptides was tested in an enzyme immunoassay (EIA). Three peptides representing residues 126-135 (close to the NH2 terminus), 309-318 (middle), and 587-596 (C-terminal) reacted with MoAb designated 48, I29, and 18, respectively. Binding of MoAb I29 to purified virus was abolished after pre-incubation with the peptide 309-318. Similarly, MoAb 48 did not bind to the virus after absorption with the peptide 126-135. Longer peptides of 19 residues from the regions reacting with the MoAb were also synthesized and tested in EIA. None of the MoAb recognized these longer peptides when the latter were bound as free peptides on solid phase. However, MoAb I29 binding to purified virus was blocked equally well by peptides 304-322 and 309-318. In contrast, peptide 121-139 absorbed the reactivity of the MoAb 48 much more weakly than the shorter peptide 126-135, suggesting that the conformation of the longer peptide in solution is different. To analyse affinities in the antigen-antibody reactions, the plates were washed with buffers of varying pH after absorption of the MoAb to MV or peptides. The MoAb I29 bound both to MV and peptide 309-318 with equal affinity, but MoAb 48 and 18 bound to the peptides 126-135 and 587-596 with lower affinity than to the virus. This study indicates that regions corresponding to amino acids 126-135, 309-318, and 587-596 define antigenic sites of the H protein.
journal_name
Scand J Immunoljournal_title
Scandinavian journal of immunologyauthors
Mäkelä MJ,Salmi AA,Norrby E,Wild TFdoi
10.1111/j.1365-3083.1989.tb01205.xsubject
Has Abstractpub_date
1989-08-01 00:00:00pages
225-31issue
2eissn
0300-9475issn
1365-3083journal_volume
30pub_type
杂志文章abstract：:The recombinant bacillus Calmette-Guérin (rBCG) secretion system utilizing an extracellular alpha antigen of Mycobacterium kansasii (alpha-K) was characterized biochemically and immunologically. The human immunodeficiency virus type 1 (HIV-1) p17gag B cell epitope fused to alpha-K was secreted in extremely large amoun...
journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：10.1046/j.1365-3083.1996.d01-28.x
更新日期：1996-02-01 00:00:00
abstract：:Mononuclear cells (MNC) eluted from rheumatoid synovial tissue of 16 patients with rheumatoid arthritis were examined for immunoglobulin-secreting cells (ISC). Immediately after elution and separation synovial tissue MNC contained considerable numbers of ISC. IgG and IgA ISC were more abundant than IgM ISC. At the sam...
journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：10.1111/j.1365-3083.1982.tb00741.x
更新日期：1982-11-01 00:00:00
abstract：:Therapy with tumour necrosis factor-alpha (TNFalpha)-blocking agents is successful in treating inflammatory disorders, but carries an increased risk of manifest and reactivating infection with intracellular bacteria. In a mouse model of latent Salmonella typhimurium infection, neutralization of TNFalpha did not result...
journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：10.1111/j.1365-3083.2007.01920.x
更新日期：2007-05-01 00:00:00
abstract：:The effector functions of IgG depend on the presence of carbohydrates attached to asparagine 297 in the Fc-portion. In this report, glycosylation profiles of recombinant wild-type and mutant IgG1 and IgG3 antibodies produced from three cell lines were analysed using LC-ESI-Orbitrap. Clear differences were detected bet...
journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：10.1111/sji.12046
更新日期：2013-05-01 00:00:00
abstract：:The study of the immunobiology of FVIII inhibitors may lead to new therapies for this potentially severe complication of haemophilia A and to new principles for the use of therapeutic proteins. In order to characterize the idiotype-anti-idiotype networks regulating FVIII inhibitors, we developed rabbit anti-idiotypic ...
journal_title：Scandinavian journal of immunology
pub_type： 社论,评审
doi：10.1111/j.1365-3083.1992.tb03125.x
更新日期：1992-11-01 00:00:00
abstract：:Serum exerts several effects in lymphocyte cultures, one of them being manifested very early. The presence of fetal calf serum (FCS) results in a markedly higher thymidine uptake within a few minutes, as compared with serum-free cultures. On the other hand, the uptake of uridine and other purine bases seem to be littl...
journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：10.1111/j.1365-3083.1981.tb00191.x
更新日期：1981-08-01 00:00:00
abstract：:In a well-established murine abortion model, stress is thought to trigger fetal rejection by inducing a proinflammatory immune response via substance P (SP), being tumour necrosis factor (TNF)-alpha-producing CD8+ T cells involved. Interestingly, the SP metabolite SP5-11 also binds to SP receptors and mediates SP-like...
journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：10.1111/j.1365-3083.2005.001711.x
更新日期：2006-01-01 00:00:00
abstract：:The function of the Fc and C3 receptors on the free surface of normal and endotoxin activated mouse peritoneal macrophages seeded on glass bound antibody--antigen (AbAg) complexes or complement was examined. We found that the glass bound AbAg complexes interfered with attachment and internalization of particles recogn...
journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：10.1111/j.1365-3083.1978.tb00422.x
更新日期：1978-01-01 00:00:00
abstract：:We have recently shown that a heat treatment of a murine target cell line, WEHI 164, induces resistance to lysis mediated by tumour necrosis factors alpha (TNF-alpha) and beta (TNF-beta). In the present study the effect of the heat shock of target cells on cytotoxicity mediated by natural killer cells (NK cells), lymp...
journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：10.1111/j.1365-3083.1990.tb02757.x
更新日期：1990-02-01 00:00:00
abstract：:The aim of the present study was to present further evidence of the specific reactivity of an anti-C3 receptor serum (AC3RS), to demonstrate membrane-bound C3 receptors by using this AC3RS in different serological an immunohistological methods, and to investigate the relationship between membrane-bound C3 receptors an...
journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：10.1111/j.1365-3083.1981.tb00112.x
更新日期：1981-01-01 00:00:00
abstract：:Tumour necrosis factor-α-induced protein-8 like-2 (TIPE2) is a newly identified immune negative regulator. The abnormal expression of TIPE2 has been found in several human inflammatory diseases. However, the expression level and clinical significance of TIPE2 in childhood asthma remain unclear. In this study, we detec...
journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：10.1111/sji.12110
更新日期：2013-12-01 00:00:00
abstract：:CD5 is a transmembrane glycoprotein on all T cells and on a subpopulation of B cells. Based on the analysis of chicken CD5-cDNA we have previously shown that the structure of the CD5 protein is conserved between species. Here we report the isolation and chromosomal mapping of the chicken CD5 gene. The gene spans 3.4 k...
journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：10.1046/j.1365-3083.2001.00961.x
更新日期：2001-07-01 00:00:00
abstract：:The in vitro anti-trinitrophenyl (TNP) response induced by TNP-lipopolysaccharide (TNP-LPs) in mouse spleen cells was eliminated by passage through Sephadex G10 columns. Conditioned medium obtained from peritoneal macrophages restored the response, indicating the supportive role of such cells in the response. Converse...
journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：10.1111/j.1365-3083.1980.tb00089.x
更新日期：1980-01-01 00:00:00
abstract：:The humoral response against the bacterial polysaccharide antigen alpha(1-->3) dextran (Dex) is controlled by J558 idiotype-(Id) specific T cells. These T cells of which the cell clone 178-4 Ts is a representative by all relevant criteria, recognize J558 Id-bearing B cells in an I-Ed-restricted manner. Costimulation v...
journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：10.1046/j.1365-3083.2001.00864.x
更新日期：2001-03-01 00:00:00
abstract：:Cells produced in murine T-cell colonies grown in methylcellulose have been studied to evaluate their proliferation, phenotypic differentiation, and ability to give rise to cytotoxic cells. Colony-forming cells from presumably cortical or medullary thymocytes, separated by peanut agglutination, or from lymph nodes, sh...
journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：10.1111/j.1365-3083.1986.tb01963.x
更新日期：1986-02-01 00:00:00
abstract：:Adaptive immunity is a complex system that appeared twice in vertebrates (in gnathostomes and in jawless fish) although it is not required for invertebrate defence. The adaptive immune system is tightly associated with self-non-self discrimination, and it is now clear that this interplay is not limited to the preventi...
journal_title：Scandinavian journal of immunology
pub_type： 杂志文章,评审
doi：10.1111/sji.12348
更新日期：2015-11-01 00:00:00
abstract：:We evaluated the B-cell response in cerebrospinal fluid (CSF) and blood by enumerating cells secreting antibodies to myelin-associated glycoprotein (MAG) and, for reference, to myelin basic protein (MBP), two myelin components which may constitute targets for autoimmune attack in multiple sclerosis (MS). Among 25 untr...
journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：10.1111/j.1365-3083.1991.tb02493.x
更新日期：1991-01-01 00:00:00
abstract：:Sea star factor (SSF) harvested from the echinoderm Asterias forbesi celomocytes is a potent inhibitor of the primary immune response to T-dependent antigens and also suppresses concanavalin-A-induced mitogenesis. We have isolated and purified a basic protein from this source which has a molecular weight of approximat...
journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：10.1111/j.1365-3083.1976.tb03037.x
更新日期：1976-01-01 00:00:00
abstract：:This study (1) analysed the percentage of γδ T cells, γδ T cell subsets, Th17 cells and regulatory T cells (Treg cells) and (2) determined the role of IL-23 in primary nephrotic syndrome (PNS) patients with active disease and in remission. Eighty-four patients with PNS and 51 healthy age-matched controls were included...
journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：10.1111/sji.12629
更新日期：2018-01-01 00:00:00
abstract：:Active chloride (Cl-) secretion by intestinal crypt enterocytes is the central pathophysiological disturbance in most cases of acute diarrhoea. We examined monolayers of the human intestinal cell line T84 mounted in Ussing chambers to see whether the T-cell lymphokine gamma interferon (IFN-gamma) might affect the Cl- ...
journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：10.1111/j.1365-3083.1989.tb02456.x
更新日期：1989-10-01 00:00:00
abstract：:Allogeneic chimaeras that utilize C.B-17 SCID mice (H-2d) as recipients of MHC mismatched bone marrow from C57B1/6 (H-2b) or SJL/J (H-2s) mice have been used to provide experimental evidence demonstrating the necessity for a direct interaction between an effector T helper and the cytotoxic T-cell precursor in order to...
journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：10.1111/j.1365-3083.1992.tb03262.x
更新日期：1992-05-01 00:00:00
abstract：:Paclitaxel (PTX) is one of the most widely used clinical antitumour drugs in chemotherapy nowadays. Its effect on immune system has become a hot spot of research in recent years. Here, we demonstrated that PTX not only decreased the percentage of CD4+Foxp3+ regulatory T (Treg) cells both in vitro and in vivo but also ...
journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：10.1111/j.1365-3083.2011.02514.x
更新日期：2011-04-01 00:00:00
abstract：:Pemphigus vulgaris is a cutaneous autoimmune disease in which the occurrence of autoantibodies directed against desmoglein-3 and other self-antigens has been well established in patient sera. However, V-region interactions (connectivity) of serum IgG and IgM have not been analysed to date. In this report, it has been ...
journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：10.1046/j.1365-3083.1999.00507.x
更新日期：1999-04-01 00:00:00
abstract：:Induced expression of the intercellular adhesion molecule 1 (ICAM-1) and of the major histocompatibility complex (MHC) class II antigens has been simultaneously observed on keratinocytes and epithelial cells in the thyroid and kidney, suggesting that ICAM-1 and HLA-DR expression might be under common regulation. We ha...
journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：10.1111/j.1365-3083.1991.tb03755.x
更新日期：1991-02-01 00:00:00
abstract：:T cells from immune guinea-pigs produce clusters in vitro with macrophages pulsed with soluble protein antigens. The formation of clusters is antigen-specific. Time-lapse cinephotomicrography was used to study the sequence of events leading to cluster formation and the kinetics of already formed clusters. The central ...
journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：10.1111/j.1365-3083.1979.tb01380.x
更新日期：1979-01-01 00:00:00
abstract：:Antigen-binding peripheral blood leukocytes (PBL) from normal and bursectomized agammaglobulinemic chickens were labeled by incubation in vitro with radioiodinated antigen at 4 degrees C in the presence of sodium azide. [125I]TGAL-binding cells could be detected by autoradiography of PBL from normal, unimmunized chick...
journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：10.1111/j.1365-3083.1975.tb02612.x
更新日期：1975-01-01 00:00:00
abstract：:Foot-and-mouth disease (FMD) caused by FMD virus (FMDV) is a major health and economic problem in the farming industry. Vaccination of livestock against this highly infectious viral disease is crucial, and inactivated FMD vaccine has been effective at controlling infection. However, accumulated data show that the inac...
journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：10.1111/sji.12349
更新日期：2015-11-01 00:00:00
abstract：:Peripheral blood mononuclear cells (PBM) from four patients with IgG myeloma and four patients with benign monoclonal gammopathy (BMG) were stimulated with pokeweed mitogen (PWM), and the in vitro immunoglobulin production over 7 days was measured with an enzyme-linked immunosorbent assay. All myeloma patients were su...
journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：10.1111/j.1365-3083.1984.tb00911.x
更新日期：1984-02-01 00:00:00
abstract：:Natural killer (NK) cell responsiveness in the mouse is determined in an education process guided by inhibitory Ly49 and NKG2A receptors binding to MHC class I molecules. It has been proposed that inhibitory signalling in human NK cells involves Abl-1 (c-Abl)-mediated phosphorylation of Crk, lowering NK cell function ...
journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：10.1111/sji.12574
更新日期：2017-09-01 00:00:00
abstract：:Twenty-eight children infected with Schistosoma haematobium and S. mansoni were tested for natural killer (NK) cell activity in vitro using the myeloid/erythroid cell line K562 as target. In addition, the frequency of large granular lymphocytes (LGL) and the number of HNK-1+ lymphocytes were examined in peripheral blo...
journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：10.1111/j.1365-3083.1986.tb01973.x
更新日期：1986-03-01 00:00:00
