Abstract:
:Serum exerts several effects in lymphocyte cultures, one of them being manifested very early. The presence of fetal calf serum (FCS) results in a markedly higher thymidine uptake within a few minutes, as compared with serum-free cultures. On the other hand, the uptake of uridine and other purine bases seem to be little influences by serum. Experiments comparing the uptake of thymidine into the cytoplasm or into trichloroacetic acid-precipitable material suggested that the intracellular thymidine pool increases in size when FCS is added. Using a Lineweaver--Burk plot for thymidine and uridine uptake over a 4-h period, no changes in uridine uptake were observed in the presence of FCS; on the contrary, serum induced an increased Vmax for thymidine, whereas Km remained constant. Cytofluorographic quantitation of G0 and G1 cells indicated that cells disappear more rapidly from the G1 phase in the presence of FCS. The addition of hydroxyurea to the cultures prevented this disappearance. The results taken together strongly suggest that serum contains a factor promoting the shift of G1 cells into the S phase. When a 4-h culture period was used, all sera from different species tested at low concentrations appeared to contain this activity.
journal_name
Scand J Immunoljournal_title
Scandinavian journal of immunologyauthors
Kristensen F,Joncourt F,de Weck ALdoi
10.1111/j.1365-3083.1981.tb00191.xsubject
Has Abstractpub_date
1981-08-01 00:00:00pages
121-30issue
2eissn
0300-9475issn
1365-3083journal_volume
14pub_type
杂志文章abstract::Co-infection with virus and bacteria happens frequently and often results in an exacerbated clinical course of the disease, possibly due to mechanisms including altered cytokine production. In the present study, the authors investigated the combined effects of avirulent Semliki Forest virus (SFV-A7) and bacterial lipo...
journal_title:Scandinavian journal of immunology
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更新日期:1997-04-01 00:00:00
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journal_title:Scandinavian journal of immunology
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doi:10.1111/j.1365-3083.1994.tb03375.x
更新日期:1994-03-01 00:00:00
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journal_title:Scandinavian journal of immunology
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更新日期:1993-01-01 00:00:00
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journal_title:Scandinavian journal of immunology
pub_type: 杂志文章,评审
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更新日期:2017-07-01 00:00:00
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journal_title:Scandinavian journal of immunology
pub_type: 杂志文章
doi:10.1046/j.1365-3083.2000.00820.x
更新日期:2000-12-01 00:00:00
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journal_title:Scandinavian journal of immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3083.1978.tb00509.x
更新日期:1978-01-01 00:00:00
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doi:10.1111/j.1365-3083.1979.tb01384.x
更新日期:1979-01-01 00:00:00
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journal_title:Scandinavian journal of immunology
pub_type: 杂志文章,评审
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更新日期:1999-01-01 00:00:00
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doi:10.1111/j.1365-3083.1977.tb02118.x
更新日期:1977-01-01 00:00:00
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journal_title:Scandinavian journal of immunology
pub_type: 杂志文章
doi:
更新日期:1985-06-01 00:00:00
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journal_title:Scandinavian journal of immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3083.1986.tb03073.x
更新日期:1986-04-01 00:00:00
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更新日期:1999-01-01 00:00:00
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journal_title:Scandinavian journal of immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3083.1976.tb00298.x
更新日期:1976-01-01 00:00:00
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journal_title:Scandinavian journal of immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3083.1981.tb00168.x
更新日期:1981-01-01 00:00:00
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journal_title:Scandinavian journal of immunology
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journal_title:Scandinavian journal of immunology
pub_type: 杂志文章
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journal_title:Scandinavian journal of immunology
pub_type: 杂志文章
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更新日期:1980-01-01 00:00:00
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journal_title:Scandinavian journal of immunology
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更新日期:2010-10-01 00:00:00
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pub_type: 杂志文章
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更新日期:1984-09-01 00:00:00
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更新日期:1995-07-01 00:00:00
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journal_title:Scandinavian journal of immunology
pub_type: 杂志文章
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更新日期:2004-02-01 00:00:00
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pub_type: 杂志文章
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更新日期:2000-07-01 00:00:00
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journal_title:Scandinavian journal of immunology
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更新日期:2002-11-01 00:00:00
abstract::Rat popliteal and the iliac lymph nodes were examined after foot-pad injections of colloidal carbon and ferritin-tetramethylrhodamine isothiocyanate. Carbon rapidly entered medullary sinuses, but the entry of carbon into the interstitium was prohibited by avid phagocytosis, by sinus macrophages and by the lymphoendoth...
journal_title:Scandinavian journal of immunology
pub_type: 杂志文章
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更新日期:1980-01-01 00:00:00