Abstract：
:Bacterial DNA differs from mammalian DNA by the presence of unmethylated cytosine-phosphate-guanosine (CpG) motifs. The immunostimulatory properties of a DNA vaccine have been suspected to be associated with these motifs. The aim of this study was to assess the inactivation of the immunostimulatory potential of a plasmid after methylation of its CpG motifs. We constructed two identical non-coding plasmids, and one of these was de novo methylated on its CG sequences. A single administration of recombinant antigen with methylated or unmethylated CpG-containing plasmid was performed in mice. As expected, only unmethylated CpG-containing plasmid enhanced the specific immune response. However, a study of in vivo activation of Langerhans' cells and analysis of mRNA synthesis indicated that both the plasmids promoted cell emigration and cytokine induction. These data highlight that a methylated CpG-containing plasmid is not inert and carries immunomodulatory properties. The results further emphasize the necessity to definitively identify the mode of action of plasmids used for DNA vaccination.
journal_name
Scand J Immunoljournal_title
Scandinavian journal of immunologyauthors
Cornélie S,Poulain-Godefroy O,Lund C,Vendeville C,Ban E,Capron M,Riveau Gdoi
10.1111/j.0300-9475.2004.01373.xkeywords:
subject
Has Abstractpub_date
2004-02-01 00:00:00pages
143-51issue
2eissn
0300-9475issn
1365-3083pii
1373journal_volume
59pub_type
杂志文章abstract：:Administration of cyclophosphamide (Cy: 150 mg/kg i.p.) to rats 48 h before immunization with a T-dependent antigen (ovalbumin) resulted in a striking absolute eosinophilia in blood, bone marrow, and secondary lymphoid organs after 10 to 14 days. This eosinophilia was preceded by a significant increase in the W3/25+/O...
journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：10.1111/j.1365-3083.1987.tb02274.x
更新日期：1987-10-01 00:00:00
abstract：:The number of CR1 on erythrocytes, as measured by monoclonal antibodies, remains undefined because of the repetitive structure of CR1 and the presence of different types of structural CR1 alleles. We studied the number of CR1 per erythrocyte using two monoclonal antibodies, E11 and 3D9, which recognize different sites...
journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：10.1111/j.1365-3083.1992.tb02948.x
更新日期：1992-07-01 00:00:00
abstract：:The stem cells for the definitive haematopoiesis are derived from intra-embryonic sources originally described in an avian model and later also in mammals. However, the molecular make-up of the early embryonic haematopoietic progenitors is not yet clearly defined. We have recently characterized the phenotype of prethy...
journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：10.1046/j.1365-3083.1998.00361.x
更新日期：1998-07-01 00:00:00
abstract：:This study investigates local alterations in T-cell and macrophage subsets that occur in cervical epithelial neoplasia (CIN), in the presence and absence of human immunodeficiency virus (HIV) infection. Ectocervical biopsies from 10 women with CIN who were infected with HIV, and 10 women with CIN but no HIV infection ...
journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：10.1046/j.1365-3083.2002.01118.x
更新日期：2002-08-01 00:00:00
abstract：:Rat popliteal and the iliac lymph nodes were examined after foot-pad injections of colloidal carbon and ferritin-tetramethylrhodamine isothiocyanate. Carbon rapidly entered medullary sinuses, but the entry of carbon into the interstitium was prohibited by avid phagocytosis, by sinus macrophages and by the lymphoendoth...
journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：10.1111/j.1365-3083.1980.tb00087.x
更新日期：1980-01-01 00:00:00
abstract：:Using rocket immunoelectrophoresis and indirect haemagglutination tests, antibodies to nicotinic acetylcholine receptor (n-AChR) from Torpedo marmorata were detected in sera from rabbits with experimental myasthenia but not in sera from patients with myasthenia gravis or in rabbit antisera to a partly purified skeleta...
journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：10.1111/j.1365-3083.1975.tb03727.x
更新日期：1975-01-01 00:00:00
abstract：:Gαq, the alpha subunit of Gq, a member of the Gq/11 sub-family, was reported to inhibit phosphatidylinositol-3-Kinase (PI3K) activation and prevent the activation of Akt. Previous studies demonstrated that mice losing Gαq in their immune system could spontaneously develop inflammatory arthritis. In this study, we show...
journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：10.1111/j.1365-3083.2011.02635.x
更新日期：2012-02-01 00:00:00
abstract：:Subclasses of lymphocytes can be separated on gradients of non-toxic polyvinylpyrrolidone-coated colloidal silica (Percoll) by virtue of differential densities. Such gradients can yield functionally active lymphocyte populations after brief centrifugation. Gradients can be generated in a discontinuous step fashion and...
journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：10.1111/j.1365-3083.1979.tb01391.x
更新日期：1979-01-01 00:00:00
abstract：:Alterations in duodenal Ig-producing cells induced by two oral cholera vaccinations were studied by two-colour immunofluorescence in mucosal tissue sections from adults with selective IgA deficiency (IgAD), either with (n = 7) or without (n = 9) frequent infections, infection-prone patients with combined IgAD and IgG ...
journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：10.1111/j.1365-3083.1993.tb01714.x
更新日期：1993-08-01 00:00:00
abstract：:The enhancement of the lytic capacity of mouse splenic 'natural killer' (NK) cells by interferon has been studied in vitro as a model for NK cell differentiation from inactive immediate precursors. We show that the increased cytotoxicity is a function of interferon concentration, and that two stages in the NK cell dif...
journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：10.1111/j.1365-3083.1980.tb00040.x
更新日期：1980-01-01 00:00:00
abstract：:We have recently established chronic relapsing experimental autoimmune encephalomyelitis (CR-EAE) in SJL mice with a modified protocol. In this model, splenectomy aborts the relapsing-remitting course of the disease, and adoptive transfer of lymphocytes of the local draining lymph nodes (LNs) to naive recipients exace...
journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：10.1046/j.1365-3083.2002.01132.x
更新日期：2002-09-01 00:00:00
abstract：:The J-chain content of 3 IgM and 24 IgA preparations was quantitated by an immunochemical technique after reduction with 20mM dithiothreitol. The amounts released ranged from undetectable (less than 0.1 mg) to 7.3 mg per 100 mg of Ig. Most of the J-chain-deficient proteins were monomeric, but four polymeric IgA prepar...
journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：10.1111/j.1365-3083.1976.tb00295.x
更新日期：1976-01-01 00:00:00
abstract：:Chromosomally marked cells from the 7-day intraembryonic mesenchyme were transplanted into 14-day-old irradiated chick embryos. At the age of 6 weeks donor-derived T and B lymphocytes were shown to be present in the thymus, spleen and bone marrow, indicating that cells in the 7-day intraembryonic mesenchyme are capabl...
journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：10.1111/j.1365-3083.1980.tb00011.x
更新日期：1980-01-01 00:00:00
abstract：:Phagocytosis of Staphylococcus aureus by human polymorphonuclear leucocytes (PMN) on the surface of endothelial cells is accompanied by adherence of the PMN to the endothelial surface and detachment of the endothelial cells from the culture monolayer. We studied the role of the leucocyte adherence-related glycoprotein...
journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：10.1111/j.1365-3083.1989.tb01192.x
更新日期：1989-07-01 00:00:00
abstract：:Primary immunodeficiency diseases represent many natural mutant models for the analysis of many aspects of immunology. Examples dealing with T- and B-cell receptors are herein discussed. They also represent privileged models in medicine, because well characterized monogenic inherited diseases can in some instances be ...
journal_title：Scandinavian journal of immunology
pub_type： 杂志文章,评审
doi：10.1046/j.1365-3083.2002.01056.x
更新日期：2002-03-01 00:00:00
abstract：:The effect of low-dose methotrexate (MTX) treatment on the CD26 density on circulating monocytes and CD4(+) T lymphocytes or levels of soluble CD26 (sCD26) has not yet been described in rheumatoid arthritis (RA). While CD26 in T lymphocytes is involved in the activation and proliferation of T lymphocytes, little is kn...
journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：10.1111/j.1365-3083.2007.01966.x
更新日期：2007-10-01 00:00:00
abstract：:We have previously shown that various benign and malignant natural killer (NK)-resistant monolayer cells inhibit endogenous human NK activity, probably by reducing the secretion of cytotoxic factors from the effector cells. The nature of the molecules responsible for the inhibition has been unclear. In this study we s...
journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：10.1111/j.1365-3083.1988.tb02411.x
更新日期：1988-07-01 00:00:00
abstract：:Human lymphocytes were cultured under different conditions to determine the effects of technical variations on the response to pokeweed mitogen (PWM) and Staphylococcus aureus as measured by (a) quantitation of immunoglobulins (Ig) in the culture supernatants and (b) enumeration of Ig-secreting cells (ISC) by a revers...
journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：10.1111/j.1365-3083.1980.tb00076.x
更新日期：1980-01-01 00:00:00
abstract：:Intradermal inoculation of mice with naked plasmid DNA encoding the regulatory HIV-1 Nef protein was shown to induce Nef-specific T and B cell responses. Co-inoculation with an expression vector encoding murine granulocyte-macrophage colony-stimulating factor (GM-CSF), a cytokine known to facilitate the induction of p...
journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：10.1046/j.1365-3083.1997.d01-130.x
更新日期：1997-09-01 00:00:00
abstract：:The main object of this study was to see whether or not chromatin constituents are present in cell membranes. The binding of antinuclear antibodies (ANA) to plasma membranes of leucocytes was studied by using autoantibodies and induced antibodies to different histones and histone peptides, and autoantibodies to double...
journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：10.1111/j.1365-3083.1989.tb01093.x
更新日期：1989-01-01 00:00:00
abstract：:Rabbit Ig against human beta2-microglobulin was found to be mitogenic for human peripheral lymphocytes, tonsil lymphocytes, and appendix and spleen cells. Anti-beta2-m gave increased DNA synthesis, with peak responses on day 3 or 4 and showed a dose-response effect when diluted. The effect was seen in both serum-free ...
journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：10.1111/j.1365-3083.1975.tb02614.x
更新日期：1975-01-01 00:00:00
abstract：:Basophilic granulocytes and mast cells of different species have been reported to originate from thymocytes and other lymphocytes. These observations were recently confirmed when evidence was given that thymic antigen is present on rabbit basophilic granulocytes. In the study reported here, human leukocytes were teste...
journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：
更新日期：1977-01-01 00:00:00
abstract：:Passive antibody can both suppress and augment immune responses. Until recently, there was virtual unanimity on the importance of the interaction of the Fc portion of modulating antibody with Fc-receptors (Fc-signalling), especially in experiments involving the suppression by antibody. Experiments reported in the last...
journal_title：Scandinavian journal of immunology
pub_type： 历史文章,杂志文章,评审
doi：10.1046/j.1365-3083.2001.00889.x
更新日期：2001-04-01 00:00:00
abstract：:Conditions for the cloning of alloactivated human lymphocytes by limiting dilution in the presence of interleukin 2 (IL 2) and filler cells have been investigated. Cloning efficiencies were extremely high (at least 40%) when IL 2 derived from pooled peripheral blood mononuclear cells (PBM), stimulated with phytohaemag...
journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：
更新日期：1981-11-01 00:00:00
abstract：:Systemic sclerosis (SSc) is a heterogeneous autoimmune disease associated with several antinuclear autoantibodies useful to diagnosis and prognosis. The aim of the present multicentric study was to determine the clinical relevance of antifibrillarin autoantibodies (AFA) in patients with SSc. The clinical features of 3...
journal_title：Scandinavian journal of immunology
pub_type： 杂志文章,多中心研究
doi：10.1111/sji.12510
更新日期：2017-01-01 00:00:00
abstract：:The advent of advanced molecular biological techniques in the last two decades has allowed the study of genetic factors in inflammatory bowel disease (IBD). A variety of techniques have been employed to elucidate the effects of genes, starting with the clinical observations that IBD is more common in the relatives of ...
journal_title：Scandinavian journal of immunology
pub_type： 杂志文章,评审
doi：10.1046/j.1365-3083.2000.00656.x
更新日期：2000-01-01 00:00:00
abstract：:Rat monoclonal antibodies directed against the BALB/c myeloma protein M315 (alpha,lambda 2) are described. 9A8 (IgG1) binds the V domain of lambda 2 and cross-reacts with lambda 1 and lambda 3 chains. 2B6 (IgG2a) is directed to the C domain of lambda 2 and cross-reacts with C lambda 3. The antibodies bind isolated cha...
journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：10.1111/j.1365-3083.1989.tb01125.x
更新日期：1989-03-01 00:00:00
abstract：:The aim of the present study was to present further evidence of the specific reactivity of an anti-C3 receptor serum (AC3RS), to demonstrate membrane-bound C3 receptors by using this AC3RS in different serological an immunohistological methods, and to investigate the relationship between membrane-bound C3 receptors an...
journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：10.1111/j.1365-3083.1981.tb00112.x
更新日期：1981-01-01 00:00:00
abstract：:Histoplasma capsulatum is a fungus found intracellularly in neutrophils and peripheral blood mononuclear cells (PBMCs), suggesting that it is capable of evading damage and survives inside these cells. In this study, we report that neutrophils from H. capsulatum-infected mice, and human neutrophils and mononuclear cell...
journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：10.1046/j.1365-3083.2002.01142.x
更新日期：2002-10-01 00:00:00
abstract：:The epidermis has been identified as an important site for the initiation of immunological events. In addition to the macrophage-like Langerhans cells, keratinocytes within the epidermal cells have been shown to act as immunoregulatory cells through the secretion of cytokines such as epidermal cell-derived thymocyte-a...
journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：10.1111/j.1365-3083.1985.tb01832.x
更新日期：1985-05-01 00:00:00
