Abstract:
AIM:The purpose of this study was to establish the influence of centrifugation and protease activity on the cerebrospinal fluid (CSF) concentrations of DJ-1 and hemoglobin. MATERIALS & METHODS:The concentrations of DJ-1 and hemoglobin were determined in 12 (DJ-1) and six (hemoglobin) pairs of CSF samples, with one sample being stored without centrifugation and the other being centrifuged at 2000 × g before storage. The DJ-1 concentration was also determined in centrifuged and uncentrifuged CSF containing protease inhibitors and compared with values determined in centrifuged and uncentrifuged CSF samples without protease inhibitors. Furthermore, specific protein concentrations were determined in CSF from two groups, each comprising 23 patients with Parkinson's disease. In one group the CSF was centrifuged at 1300-1800 × g, 4°C, 10 min, and in the other at 2000 × g, 4°C, 10 min. RESULTS:Centrifugation at 2000 × g resulted in significantly lower CSF DJ-1 concentrations compared with no centrifugation and centrifugation at a lower g-force. There was a significant difference in the hemoglobin concentration between centrifuged and uncentrifuged CSF. In all centrifuged samples the hemoglobin concentration was <200 ng/ml including blood contaminated samples centrifuged at 2000 × g. When a protease inhibitor cocktail was added to the CSF prior to centrifugation, the DJ-1 concentration was significantly higher. CONCLUSION:Preanalytical factors such as centrifugation and protease inhibition must be carefully controlled when handling CSF for analysis of DJ-1 and other biomarkers, as DJ-1 was influenced by blood contamination, centrifugation and protease activity.
journal_name
Biomark Medjournal_title
Biomarkers in medicineauthors
Salvesen L,Tanassi JT,Bech S,Pålhagen S,Svenningsson P,Heegaard NH,Winge Kdoi
10.2217/bmm.13.125subject
Has Abstractpub_date
2014-01-01 00:00:00pages
387-94issue
3eissn
1752-0363issn
1752-0371journal_volume
8pub_type
杂志文章abstract::Increased neurohormonal activation is a key feature of heart failure (HF). Copeptin is a surrogate marker for proarginine vasopressin and the prognostic value of copeptin has been reported for multiple disease states of both nonvascular and cardiovascular etiology. Elevated plasma copeptin in HF has been associated wi...
journal_title:Biomarkers in medicine
pub_type: 杂志文章,评审
doi:10.2217/bmm.14.50
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journal_title:Biomarkers in medicine
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journal_title:Biomarkers in medicine
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abstract::Letter in regards to: Akboga MK, Balci KG, Maden O et al. Usefulness of monocyte to HDL-cholesterol ratio to predict high SYNTAX score in patients with stable coronary artery disease. Biomark. Med. 10(4), 375-383 (2016). ...
journal_title:Biomarkers in medicine
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abstract::This article focuses on biochemical markers that may be used in the diagnostics of Parkinson's disease and associated disorders, and to identify early cases and stratify patients into subgroups. We present an updated account of some currently available candidate fluid biomarkers, and discuss their diagnostic performan...
journal_title:Biomarkers in medicine
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journal_title:Biomarkers in medicine
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journal_title:Biomarkers in medicine
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journal_title:Biomarkers in medicine
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journal_title:Biomarkers in medicine
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abstract::Acute kidney injury is a common and significant complication among pediatric patients with congenital heart disease, occurring most commonly after cardiopulmonary bypass. Current laboratory methods of diagnosis are not timely enough to guide management decisions, thus spurring interest in discovering new biomarkers of...
journal_title:Biomarkers in medicine
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journal_title:Biomarkers in medicine
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journal_title:Biomarkers in medicine
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journal_title:Biomarkers in medicine
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journal_title:Biomarkers in medicine
pub_type: 杂志文章
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更新日期:2010-08-01 00:00:00
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journal_title:Biomarkers in medicine
pub_type: 杂志文章
doi:10.2217/bmm-2019-0391
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journal_title:Biomarkers in medicine
pub_type: 杂志文章
doi:10.2217/bmm-2017-0102
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journal_title:Biomarkers in medicine
pub_type: 杂志文章
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journal_title:Biomarkers in medicine
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journal_title:Biomarkers in medicine
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journal_title:Biomarkers in medicine
pub_type: 杂志文章,评审
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更新日期:2011-12-01 00:00:00
abstract::Coronary heart disease (CHD) is the main cause of death in post-menopausal women (PMW). Beyond the 'traditional' cardiovascular risk factors of CHD, newer biomarkers, reflecting inflammation, endothelial function and oxidative stress, have received growing consideration. We systematically reviewed the literature on th...
journal_title:Biomarkers in medicine
pub_type: 杂志文章,meta分析,评审
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更新日期:2011-08-01 00:00:00
abstract::In general, a biomarker has multiple uses such as a diagnostic tool and a method to monitor therapy. The quality of a biomarker depends on how big the difference is between, for example, patients and healthy controls, but also on the capacity of the method used to measure it (the uncertainty in the method should be mu...
journal_title:Biomarkers in medicine
pub_type: 杂志文章,评审
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更新日期:2012-08-01 00:00:00
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journal_title:Biomarkers in medicine
pub_type: 杂志文章
doi:10.2217/bmm-2017-0187
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abstract:AIM:To investigate the clinical significance of detecting several biomarkers collectively in the diagnosis of rheumatoid arthritis (RA). METHODS:128 RA patients, 174 non-RA patients and 80 healthy controls were enrolled. HLA-DR4 and HLA-DR53 were detected by the PCR-SSP method, 14-3-3η protein, anti-CCP and anti-Sa we...
journal_title:Biomarkers in medicine
pub_type: 杂志文章
doi:10.2217/bmm-2017-0371
更新日期:2018-07-01 00:00:00
abstract::Combination antiretroviral therapy (cART) has significantly reduced morbidity and mortality of HIV-infected patients, yet their life expectancy remains reduced compared with the general population. Most HIV-infected patients receiving cART have some persistent immune dysfunction characterized by chronic immune activat...
journal_title:Biomarkers in medicine
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journal_title:Biomarkers in medicine
pub_type: 杂志文章
doi:10.2217/bmm.15.95
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journal_title:Biomarkers in medicine
pub_type: 杂志文章
doi:10.2217/bmm-2016-0238
更新日期:2017-03-01 00:00:00