Abstract:
OBJECTIVES:Root canal treatment failures often correlate with persistent biomaterial-associated endodontic infections. The aim of the present study was to assess the impact of endodontic obturation material sampling from root canals with posttreatment apical periodontitis on improving standard study protocols. MATERIALS AND METHODS:Samples from previously filled root canals and their corresponding endodontic filling materials were obtained from five root-filled teeth with posttreatment periradicular lesions. After cultivation, the isolated microorganisms were quantified and biochemically identified. Moreover, clone libraries were constructed after the amplification of bacterial 16S ribosomal DNA (rDNA) from the same samples. DNA from selected clones was sequenced to identify microbial species. Transmission electron microscopy (TEM) aided visualization of the detected bacteria. RESULTS:Overall, 22 taxa of the phyla Firmicutes, Actinobacteria, and Bacteroidetes were detected in both obturation and root canal samples by culture-dependent and culture-independent methods. Root canal fillings sheltered 17 species (3.30-7.50 × 10(3) CFU/ml). Of these, nine were detected solely in the retrieved obturation materials. The reinfected root canals harbored 13 taxa (3.48-7.36 × 10(3) CFU/ml). Obligate and facultative anaerobic bacteria prevailed. The number of different species ranged from 1 to 5 within a single sample. Fungi were not detected. CONCLUSIONS:Bacteria can colonize both root canals and endodontic fillings in vivo. CLINICAL RELEVANCE:Integrating the sampling of obturation materials with standard root canal sample collection offers a clearer insight into the actual microbial flora of reinfected root canals and improves the study protocols of secondary/persistent endodontic infections.
journal_name
Clin Oral Investigjournal_title
Clinical oral investigationsauthors
Karygianni L,Anderson AC,Tennert C,Kollmar K,Altenburger MJ,Hellwig E,Al-Ahmad Adoi
10.1007/s00784-014-1231-4subject
Has Abstractpub_date
2015-03-01 00:00:00pages
319-27issue
2eissn
1432-6981issn
1436-3771journal_volume
19pub_type
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journal_title:Clinical oral investigations
pub_type: 杂志文章
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更新日期:2020-09-01 00:00:00
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journal_title:Clinical oral investigations
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journal_title:Clinical oral investigations
pub_type: 杂志文章,随机对照试验
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更新日期:2019-09-01 00:00:00
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pub_type: 杂志文章
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更新日期:2014-07-01 00:00:00
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journal_title:Clinical oral investigations
pub_type: 杂志文章
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更新日期:2020-08-22 00:00:00
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pub_type: 杂志文章
doi:10.1007/s00784-019-02820-4
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pub_type: 杂志文章
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更新日期:2012-02-01 00:00:00
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pub_type: 杂志文章
doi:10.1007/s00784-013-0985-4
更新日期:2014-01-01 00:00:00
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pub_type: 杂志文章
doi:10.1007/s00784-015-1453-0
更新日期:2015-12-01 00:00:00
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pub_type: 杂志文章
doi:10.1007/s00784-011-0605-0
更新日期:2012-08-01 00:00:00
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pub_type: 杂志文章
doi:10.1007/s00784-013-1114-0
更新日期:2014-01-01 00:00:00
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pub_type: 杂志文章
doi:10.1007/s00784-014-1314-2
更新日期:2015-06-01 00:00:00
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pub_type: 杂志文章
doi:10.1007/s00784-020-03616-7
更新日期:2020-10-13 00:00:00
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pub_type: 杂志文章
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更新日期:2016-09-01 00:00:00
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pub_type: 杂志文章
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pub_type: 临床试验,杂志文章,随机对照试验
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更新日期:1998-06-01 00:00:00
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更新日期:2019-02-01 00:00:00
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journal_title:Clinical oral investigations
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更新日期:2012-06-01 00:00:00
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journal_title:Clinical oral investigations
pub_type: 杂志文章
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更新日期:2018-06-01 00:00:00
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journal_title:Clinical oral investigations
pub_type: 杂志文章,随机对照试验
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更新日期:2009-03-01 00:00:00
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journal_title:Clinical oral investigations
pub_type: 临床试验,杂志文章,随机对照试验
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更新日期:2001-12-01 00:00:00
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pub_type: 杂志文章
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更新日期:2016-09-01 00:00:00
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pub_type: 杂志文章
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更新日期:2017-12-01 00:00:00
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journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-015-1514-4
更新日期:2016-03-01 00:00:00
abstract:OBJECTIVES:To explore the pathophysiology of proliferative verrucous leukoplakia, a rare oral disorder that exhibits high rates of recurrence and malignant transformation, through a RNAseq case-control study. MATERIAL AND METHODS:We obtained oral biopsies from 10 patients with verrucous leukoplakia lesions and from th...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-020-03575-z
更新日期:2020-09-11 00:00:00
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pub_type: 杂志文章
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更新日期:2016-06-01 00:00:00