Abstract:
:We determined the effects of soluble or coated nanocrystalline hydroxyapatite paste (nano-HA) and enamel matrix derivative (EMD) on proliferation, adhesion, and migration of periodontal ligament fibroblasts (PDLs). Cultured PDLs were stimulated with nano-HA paste or EMD in a soluble form or were coated to the surface of cell culture dishes. Proliferation of PDLs on coated nano-HA and EMD was quantified by various methods including bromodeoxyuridine (BrdU) incorporation and Western blot. Cell migration was investigated in a modified Boyden chamber. The surface integrin profile of PDLs was determined using an integrin-specific ELISA, and integrin-specific signaling was measured by immunoblotting of phosphorylated focal adhesion kinase (FAK). Coated nano-HA stimulated PDL proliferation to a larger extent as compared with coated EMD. PDL migration towards a nano-HA or EMD gradient was more efficiently mediated by soluble EMD as compared with nano-HA but vice versa, adhesion of PDLs to compound-coated dishes was more effectively mediated by nano-HA as compared with EMD. Mechanistically, majorly integrin α5β1-mediated adhesion of PDL and both coated compounds mediated a significant increase in FAK activation though to a different extent. Current findings offer two different modes of action for EMD and nano-HA paste. EMD efficiently acts as a chemoattractant in its soluble form, while nano-HA paste effectively serves as a synthetic extracellular matrix component in its coated form. Our findings suggest that EMD and nano-HA paste display different molecular characteristics and apply alternative routes to mediate their beneficial effects on periodontal tissues.
journal_name
Clin Oral Investigjournal_title
Clinical oral investigationsauthors
Kasaj A,Willershausen B,Junker R,Stratul SI,Schmidt Mdoi
10.1007/s00784-011-0570-7subject
Has Abstractpub_date
2012-06-01 00:00:00pages
745-54issue
3eissn
1432-6981issn
1436-3771journal_volume
16pub_type
杂志文章abstract::At a dental office in Münster, Germany, the majority of gold inlays incorporated between July 1969 and August 1999 (n=2,071) in 531 patients were checked with reference to the dental records and assessed retrospectively to determine their longevity. One hundred seventy-five gold inlays were rated as failures (no longe...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s007840100119
更新日期:2001-09-01 00:00:00
abstract:OBJECTIVES:The purpose of this study was to analyze the prevalence of dental erosion among competitive swimmers of the local swimming club in Szczecin, Poland, who train in closely monitored gas-chlorinated swimming pool water. MATERIALS AND METHODS:The population for this survey consisted of a group of junior competi...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-012-0720-6
更新日期:2013-03-01 00:00:00
abstract:OBJECTIVES:This study aimed to conduct a methodological assessment of paper-based systematic reviews (SR) published in oral health using a validated checklist. A secondary objective was to explore temporal trends on methodological quality. MATERIAL AND METHODS:Two electronic databases (OVID Medline and OVID EMBASE) we...
journal_title:Clinical oral investigations
pub_type: 杂志文章,评审
doi:10.1007/s00784-015-1663-5
更新日期:2016-04-01 00:00:00
abstract:OBJECTIVES:Analgesics are one of the most frequently used medicines. Self-medication and misuse have been described in the literature. The purpose of this study was to document analgesic (mis)use in a population seeking emergency dental treatment. MATERIAL AND METHODS:Patients consulting a dental emergency service wer...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-017-2228-6
更新日期:2018-04-01 00:00:00
abstract:OBJECTIVE:Ex-vivo evaluation of the detectability of vertical root fractures (VRFs) using digital subtraction radiography (DSR) and conventional digital periapical radiography (CDPR); investigation of the effect of root canal filling, x-ray angulation, and thickness of the VRF in the diagnostic accuracy. MATERIALS AND...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-020-03245-0
更新日期:2020-10-01 00:00:00
abstract:OBJECTIVES:This study was designed to investigate the antimicrobial properties, compressive strength and fluoride release capacities of high-viscous glass ionomer cements (GICs) after incorporation of cinnamon and thyme essential oils. MATERIALS AND METHODS:Experimental-modified GICs were prepared by incorporation of ...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-020-03493-0
更新日期:2020-08-15 00:00:00
abstract:OBJECTIVE:Dental materials and oral hygiene products may be responsible for oral contact allergic reactions. We aimed to determine the occurrence of allergies in patients with symptomatic oral lichen planus (OLP), oral lichenoid lesions (OLLs) and stomatitis and investigate if patch testing could identify contact aller...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-017-2053-y
更新日期:2017-11-01 00:00:00
abstract::The aim of the study was the morphological analysis of coronal dentin caries and the modifications induced by different pretreatments with phosphoric acid or sodium hypochlorite. Carious dentin specimens were obtained from human molars affected by carious lesions. Specimens were divided in four groups and submitted to...
journal_title:Clinical oral investigations
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1007/s00784-003-0221-8
更新日期:2003-09-01 00:00:00
abstract:OBJECTIVES:Although dental trauma and its unfavorable sequelae are considered major public health problems worldwide, the published data on the prevalence of traumatic crown injuries (TCIs) in Germany are lacking. Therefore, the present study assessed the prevalence of TCIs among adolescents in Bavaria, Germany. MATER...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-019-02974-1
更新日期:2020-02-01 00:00:00
abstract:INTRODUCTION:Decay of teeth, erosion and disintegration of hard dental tissue are a clinically often seen side effect after radiotherapy for tumours in the head and neck region. To investigate if these side effects are due to structural changes in dental hard tissues, the current literature was searched. It can influen...
journal_title:Clinical oral investigations
pub_type: 杂志文章,评审
doi:10.1007/s00784-013-1034-z
更新日期:2014-01-01 00:00:00
abstract:OBJECTIVES:The aim of this retrospective cephalometric study was to compare treatment outcomes with "bite jumping appliance" (BJA) or Andresen-Häupl type activator. It especially focused on skeletal and dental structures in patients with class II malocclusion. The study hypothesis was that differences in treatment-rela...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-019-03115-4
更新日期:2020-07-01 00:00:00
abstract:OBJECTIVES:Geranylgeraniol (GGOH) has been reported as a potential treatment option for bisphosphonate-associated osteonecrosis of the jaws (BP-ONJ). The aim of this study was to analyze the effects of GGOH on endothelial progenitor cells (EPC) after bisphosphonate treatment in vitro. MATERIALS AND METHODS:EPC were in...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-014-1394-z
更新日期:2015-09-01 00:00:00
abstract:AIM:The primary objective of this study was (1) to evaluate the 5-year clinical outcome of regenerative periodontal therapy (RPT) using minimally invasive surgery and a collagen-enriched bovine-derived xenograft and (2) to identify predictors for clinical attachment level (CAL) gain and vertical radiographic bone (RB) ...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-017-2208-x
更新日期:2018-04-01 00:00:00
abstract:OBJECTIVES:The present case-control study aims to assess the occurrence of alexithymic traits in burning mouth syndrome (BMS) subjects and to correlate alexithymic traits to anxious and depressive traits in BMS subjects. MATERIALS AND METHODS:Prospectively enrolled BMS and control subjects were administered the 20-ite...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-015-1416-5
更新日期:2015-11-01 00:00:00
abstract:OBJECTIVES:The aim of this systematic review was to assess the efficacy of bioactive glass (BG) in bone regeneration for implant site development procedures. MATERIAL AND METHODS:The following specific question was formulated with reference to Population, Intervention, Control, Outcomes (PICO): "In persons that underg...
journal_title:Clinical oral investigations
pub_type: 杂志文章,评审
doi:10.1007/s00784-014-1376-1
更新日期:2015-03-01 00:00:00
abstract:OBJECTIVES:Evaluate the bone remodeling during orthodontic movement with corticotomy when submitted to low-intensity electrical stimulation application (microcurrent-MC) and low-level laser therapy (LLLT). MATERIAL AND METHODS:One hundred and fifty Wistar rats were divided into the following 5 groups: (C) submitted to...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-019-02845-9
更新日期:2019-11-01 00:00:00
abstract::This study deals with 4971 implants (Brånemark system) installed in 1315 patients, either fully or partially edentulous, and followed from implant installation up to the last control. A predominance of female patients (61%) and a nearly equal number of upper and lower jaws characterised the study group. Patients were ...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s007840050107
更新日期:2000-03-01 00:00:00
abstract:OBJECTIVES:To assess the potential influence of systemic antibiotic administration on the healing of periodontal intrabony defects treated with deproteinized bovine bone mineral (DBBM) and collagen membrane. MATERIALS AND METHODS:Forty-one intrabony defects were treated by means of DBBM and collagen membrane (GTR). Po...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-020-03616-7
更新日期:2020-10-13 00:00:00
abstract:OBJECTIVES:The aim of this study was to compare the effects of periodontal treatment on the inflammatory markers in gingival crevicular fluid and the concentration of salivary cortisol between non-pregnant and pregnant women with gingivitis. MATERIALS AND METHODS:This study included 30 non-pregnant women (mean age 27....
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-018-2569-9
更新日期:2018-07-31 00:00:00
abstract::The goal of this investigation was to evaluate the effect of postoperative exposure of two different bioresorbable membranes on the guided tissue regeneration (GTR) healing results compared to nonexposed sites. In each of 25 patients one pair of contralateral intrabony lesions was treated either with polylactic acid (...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s007840050021
更新日期:1997-09-01 00:00:00
abstract::This paper presents a short review of three groups of tools which can be or are used for the tissue engineering of mineralized oral structures: growth factor delivery systems (GFDS) and surface bioactivation with covalent bound peptides or with nanomechanically linked proteins. According to the reported personal exper...
journal_title:Clinical oral investigations
pub_type: 杂志文章,评审
doi:10.1007/s007840050128
更新日期:2000-06-01 00:00:00
abstract:OBJECTIVES:Intermittent parathyroid hormone (PTH) exerts anabolic effects on bone and has been approved for osteoporosis therapy. The dual actions of PTH are mediated primarily through the parathyroid hormone 1 receptor (PTH1R). Upon ligand binding, PTH1R activates diverse signaling pathways, including cAMP/protein kin...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-013-0985-4
更新日期:2014-01-01 00:00:00
abstract:OBJECTIVES:Recent scientific evidences suggest a relevant role of the oral cavity in the transmission and pathogenicity of SARS-CoV-2. METHODS:A literature search was performed in PubMed, up to April 30, 2020, focusing on SARS-CoV-2, COVID-19, oral cavity, and antimicrobial agents. RESULTS:Oral viral load of SARS-CoV...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-020-03413-2
更新日期:2020-08-01 00:00:00
abstract:OBJECTIVES:To evaluate the mRNA expression levels of cytokines interferon-γ, tumour necrosis factor-α, interleukin IL-1β, IL-10, and the chemokine CCL2/MCP-1, CCL4, and CXCR4 in the periapical interstitial fluid from root canal infections before and after bacterial load reduction in patients undergoing haematopoietic s...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-020-03448-5
更新日期:2020-07-14 00:00:00
abstract:OBJECTIVES:The aim of this study is to investigate the relationship between periodontal morphometric parameters and to develop a biotype classification based on the variables examined with the use of cone-beam computed tomography (CBCT). MATERIALS AND METHODS:Forty-two periodontally healthy subjects that underwent a C...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-015-1694-y
更新日期:2016-11-01 00:00:00
abstract:OBJECTIVES:Periodontitis is associated with systemic inflammation, elevated platelet activation and enhanced risk for cardiovascular diseases, while periodontal treatment reduces tissue inflammation and shows desirable effects on the oral biofilm and dental health. However, subgingival debridement during conservative t...
journal_title:Clinical oral investigations
pub_type: 临床试验,杂志文章
doi:10.1007/s00784-019-03049-x
更新日期:2020-05-01 00:00:00
abstract:OBJECTIVES:The objective of this study was to evaluate the efficacy of four different side-to-side toothbrushes and the impact of various brushing parameters on noncontact biofilm removal in an adjustable interdental space model. MATERIALS AND METHODS:A three-species biofilm, consisting of Porphyromonas gingivalis, Fu...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-016-1969-y
更新日期:2017-06-01 00:00:00
abstract:OBJECTIVES:The aim of the present study was the qualitative and quantitative evaluation of osseous graft consolidation using allogeneic bone blocks for vertical bone augmentation in an animal model. MATERIAL AND METHODS:Standardised allogeneic and autologous bone blocks were fixed on the frontal skull of 20 adult fema...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-019-03151-0
更新日期:2020-08-01 00:00:00
abstract::Resin-bonded fixed partial dentures (RBFPD) are used as a minimal invasive, tooth-preventing alternative for replacing anterior teeth. Zirconia cantilever restorations were supposed to show sufficient strength for a clinical application. The aim of this investigation was to determine the fracture characteristics of ca...
journal_title:Clinical oral investigations
pub_type: 杂志文章,随机对照试验
doi:10.1007/s00784-009-0254-8
更新日期:2009-12-01 00:00:00
abstract:OBJECTIVE:To describe and explore the association between proxy-reported sleep bruxism (SB) and quality of life (QoL) in a population of Colombian children belonging to different social layers. METHODS:The parents of 1556, 6-13-year-old school children, were administered the Pediatric Inventory of Quality of Life (Ped...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-016-1901-5
更新日期:2017-05-01 00:00:00