Exchange protein directly activated by cAMP plays a critical role in bacterial invasion during fatal rickettsioses.

abstract：:CD31 is an Ig-like molecule expressed by leukocytes and endothelial cells with an established role in the regulation of leukocyte trafficking. Despite genetic deletion of CD31 being associated with exacerbation of T cell-mediated autoimmunity, the contribution of this molecule to T-cell responses is largely unknown. H...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ma L,Mauro C,Cornish GH,Chai JG,Coe D,Fu H,Patton D,Okkenhaug K,Franzoso G,Dyson J,Nourshargh S,Marelli-Berg FM

更新日期：2010-11-09 00:00:00

abstract：:Explosions account for 79% of combat-related injuries, leading to multiorgan hemorrhage and uncontrolled bleeding. Uncontrolled bleeding is the leading cause of death in battlefield traumas as well as in civilian life. We need to stop the bleeding quickly to save lives, but, shockingly, there are no treatments to stop...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lashof-Sullivan MM,Shoffstall E,Atkins KT,Keane N,Bir C,VandeVord P,Lavik EB

更新日期：2014-07-15 00:00:00

abstract：:Spantide (D-Arg1-Pro2-Lys3-Pro4-Gln5-Gln6-D-Trp7-Phe8-D-Trp9-++ +Leu10-Leu11-NH2) was introduced as a tachykinin antagonist in 1984 and has served as a starting point in the design of new antagonists that have proven to be more effective and have exhibited no neurological side effects. The most remarkable and unpredic...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Folkers K,Feng DM,Asano N,Håkanson R,Weisenfeld-Hallin Z,Leander S

更新日期：1990-06-01 00:00:00

abstract：:4"'-Diazomalonyldigitoxin and its isomer, 3"'-diazomalonyldigitoxin, have been synthesized at high specific radioactivity and used as photolabels for the Na,K-ATPase (ATP phosphohydrolase, EC 3.6.1.3) purified from Electrophorus electricus. Photoaffinity labeling experiments using both type I and type II complexes of ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hall C,Ruoho A

更新日期：1980-08-01 00:00:00

abstract：:Biogeochemical reactions occur unevenly in space and time, but this heterogeneity is often simplified as a linear average due to sparse data, especially in subsurface environments where access is limited. For example, little is known about the spatial variability of groundwater denitrification, an important process in...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kolbe T,de Dreuzy JR,Abbott BW,Aquilina L,Babey T,Green CT,Fleckenstein JH,Labasque T,Laverman AM,Marçais J,Peiffer S,Thomas Z,Pinay G

更新日期：2019-02-12 00:00:00

abstract：:Targeted drug delivery critically depends on the binding selectivity of cargo-transporting colloidal particles. Extensive theoretical work has shown that two factors are necessary to achieve high selectivity for a threshold receptor density: multivalency and weak interactions. Here, we study a model system of DNA-coat...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Scheepers MRW,van IJzendoorn LJ,Prins MWJ

更新日期：2020-09-15 00:00:00

abstract：:p300 acetylates and activates the tumor suppressor p53 after DNA damage. Here, we show that MDM2, a negative-feedback regulator of p53, inhibited p300-mediated p53 acetylation by complexing with these two proteins. First, we purified a p300-MDM2-p53 protein complex from HeLa nuclear extracts, which was inactive in p53...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kobet E,Zeng X,Zhu Y,Keller D,Lu H

更新日期：2000-11-07 00:00:00

abstract：:We purified microtubules from a mammalian mitotic extract and obtained an amino acid sequence from each microtubule-associated protein by using mass spectrometry. Most of these proteins are known spindle-associated components with essential functional roles in spindle organization. We generated antibodies against a pr...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mack GJ,Compton DA

更新日期：2001-12-04 00:00:00

abstract：:When the content of cyclic AMP (cAMP) was compared in livers of a series of congenic mouse strains differing at the H-2 locus, significant variation in concentration of cAMP per unit wet weight was found among strains, and also for animals of a given strain with increasing age. For a given age, from 8 to 22 weeks, cAM...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Meruelo D,Edidin M

更新日期：1975-07-01 00:00:00

abstract：:Disordered systems like liquids, gels, glasses, or granular materials are not only ubiquitous in daily life and in industrial applications, but they are also crucial for the mechanical stability of cells or the transport of chemical and biological agents in living organisms. Despite the importance of these systems, th...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zhang Z,Kob W

更新日期：2020-06-23 00:00:00

abstract：:Plants in dense vegetation compete for resources, including light, and optimize their growth based on neighbor detection cues. The best studied of such behaviors is the shade-avoidance syndrome that positions leaves in optimally lit zones of a vegetation. Although proximate vegetation is known to be sensed through a r...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： de Wit M,Kegge W,Evers JB,Vergeer-van Eijk MH,Gankema P,Voesenek LA,Pierik R

更新日期：2012-09-04 00:00:00

abstract：:We studied the abundance and structure of globin mRNAs present in K562 cells both before and after induction of hemoglobin synthesis by hemin. In vitro translation of poly(A)+ RNA from K562 cells generated protein products corresponding to alpha, A gamma-, G gamma-, epsilon-, and zeta-globin mRNAs. Individual globin m...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Dean A,Ley TJ,Humphries RK,Fordis M,Schechter AN

更新日期：1983-09-01 00:00:00

abstract：:Selection is usually considered to be the dominant force controlling viral variation; the large population sizes suggest that deterministic population genetic models are appropriate. To investigate their validity for HIV, samples of env gene sequences were tested for departure from neutrality because of mutation-selec...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Brown AJ

更新日期：1997-03-04 00:00:00

abstract：:A rigorous numerical test of a hypothetical mechanism of a molecular motor should model explicitly the diffusive motion of the motor's degrees of freedom as well as the transition rates between the motor's chemical states. We present such a Brownian dynamics, mechanochemcial model of the coarse-grain structure of the ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Craig EM,Linke H

更新日期：2009-10-27 00:00:00

abstract：:Lipid-mediated signaling events regulate many cellular processes. Investigations of the complex underlying mechanisms are difficult because several different methods need to be used under varying conditions. Here we introduce multifunctional lipid derivatives to study lipid metabolism, lipid-protein interactions, and ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Höglinger D,Nadler A,Haberkant P,Kirkpatrick J,Schifferer M,Stein F,Hauke S,Porter FD,Schultz C

更新日期：2017-02-14 00:00:00

abstract：:The detrimental effects of elevated intensities of mid-UV radiation (UVB), a result of stratospheric ozone depletion during the austral spring, on the primary producers of the Antarctic marine ecosystem have been well documented. Here we report that natural populations of Antarctic zooplankton also sustain significant...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Malloy KD,Holman MA,Mitchell D,Detrich HW 3rd

更新日期：1997-02-18 00:00:00

abstract：:Brood parasitism as an alternative female breeding tactic is particularly common in ducks, where hosts often receive eggs laid by parasitic females of the same species and raise their offspring. Herein, we test several aspects of a kin selection explanation for this phenomenon in goldeneye ducks (Bucephala clangula) b...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Andersson M,Ahlund M

更新日期：2000-11-21 00:00:00

abstract：:Spleen cells derived from a rabbit hyperimmunized with a Type III pneumococcal vaccine were exposed to simian virus 40 in vitro. After 114 days, transformed cells growing on the surface of one culture dish were observed and a cell line was established. The transformed cells had a morphology characteristic of cells tra...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Collins JJ,Black PH,Strosberg AD,Haber E,Bloch KJ

更新日期：1974-02-01 00:00:00

abstract：:TAL1 is a critical transcription factor required for hematopoiesis. However, perturbation of its activity often leads to T cell leukemia. Whether and how its transcriptional activities are regulated during hematopoiesis remains to be addressed. Here, we show that TAL1 is associated with histone demethylase complexes c...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hu X,Li X,Valverde K,Fu X,Noguchi C,Qiu Y,Huang S

更新日期：2009-06-23 00:00:00

abstract：:The Max gene encodes a protein that interacts specifically with the Myc protein to form a heterodimer with high affinity for the specific cognate DNA binding site of Myc. Here we examine the expression of Max RNA in comparison to Myc RNA during cell growth and differentiation. Two species of RNA, a major 2.0- and a mi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wagner AJ,Le Beau MM,Diaz MO,Hay N

更新日期：1992-04-01 00:00:00

abstract：:In enteric bacteria, the cellular response to oxidative stress is activated by oxidation of the iron-sulfur clusters in SoxR, which then induces transcription of soxS, turning on a battery of defense genes. Here we demonstrate both in vitro and in cells that activation of SoxR can occur in a DNA-mediated reaction with...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lee PE,Demple B,Barton JK

更新日期：2009-08-11 00:00:00

abstract：:The extracellular hemoglobin of Ascaris has an extremely high oxygen affinity (P50 = 0.004 mmHg). It consists of eight identical subunits of molecular weight 40,600. Their sequence, determined by protein chemistry, shows two tandemly linked globin-like sequences and an 18-residue C-terminal extension. Two N-linked gly...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： De Baere I,Liu L,Moens L,Van Beeumen J,Gielens C,Richelle J,Trotman C,Finch J,Gerstein M,Perutz M

更新日期：1992-05-15 00:00:00

abstract：:The DnaE intein in Synechocystis sp. strain PCC6803 is the first and only naturally split intein that has been identified so far. It is capable of catalyzing a protein trans-splicing mechanism to assemble a mature protein from two separate precursors. Therefore, it is a powerful tool for protein modification and engin...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yang J,Fox GC Jr,Henry-Smith TV

更新日期：2003-03-18 00:00:00

abstract：:Variants of isovaleryl-CoA dehydrogenase (IVDHase, EC 1.3.99.10) in 15 isovaleric acidemia fibroblast lines were analyzed using [35S]methionine labeling, immunoprecipitation with anti-rat IVDHase antiserum, and NaDodSo4/polyacrylamide gel electrophoresis. Five distinct variants of IVDHase were detected. The molecular ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ikeda Y,Keese SM,Tanaka K

更新日期：1985-10-01 00:00:00

abstract：:The sources of human germline mutations are poorly understood. Part of the difficulty is that mutations occur very rarely, and so direct pedigree-based approaches remain limited in the numbers that they can examine. To address this problem, we consider the spectrum of low-frequency variants in a dataset (Genome Aggreg...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Agarwal I,Przeworski M

更新日期：2019-09-03 00:00:00

abstract：:Homeostatic mechanisms are required to control formation and maintenance of synaptic connections to maintain the general level of neural impulse activity within normal limits. How genes controlling these processes are co-coordinately regulated during homeostatic synaptic plasticity is unknown. MicroRNAs (miRNAs) exert...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Cohen JE,Lee PR,Chen S,Li W,Fields RD

更新日期：2011-07-12 00:00:00

abstract：:Banded iron formations (BIFs) record a time of extensive Fe deposition in the Precambrian oceans, but the sources and pathways for metals in BIFs remain controversial. Here, we present Fe- and Nd-isotope data that indicate two sources of Fe for the large BIF units deposited 2.5 billion y ago. High-εNd and -δ(56)Fe sig...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Li W,Beard BL,Johnson CM

更新日期：2015-07-07 00:00:00

abstract：:There are defined medullary, mesencephalic, hypothalamic, and thalamic functions in regulation of respiration, but knowledge of cortical control and the elements subserving the consciousness of breathlessness and air hunger is limited. In nine young adults, air hunger was produced acutely by CO(2) inhalation. Comparis...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Brannan S,Liotti M,Egan G,Shade R,Madden L,Robillard R,Abplanalp B,Stofer K,Denton D,Fox PT

更新日期：2001-02-13 00:00:00

abstract：:Organic hydroperoxide resistance (Ohr) enzymes are unique Cys-based, lipoyl-dependent peroxidases. Here, we investigated the involvement of Ohr in bacterial responses toward distinct hydroperoxides. In silico results indicated that fatty acid (but not cholesterol) hydroperoxides docked well into the active site of Ohr...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Alegria TG,Meireles DA,Cussiol JR,Hugo M,Trujillo M,de Oliveira MA,Miyamoto S,Queiroz RF,Valadares NF,Garratt RC,Radi R,Di Mascio P,Augusto O,Netto LE

更新日期：2017-01-10 00:00:00

abstract：:Rett syndrome (RTT) is a severe neurodevelopmental disorder that affects about 1 in 10,000 female live births. The underlying cause of RTT is mutations in the X-linked gene, methyl-CpG-binding protein 2 (MECP2); however, the molecular mechanism by which these mutations mediate the RTT neuropathology remains enigmatic....

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Osenberg S,Karten A,Sun J,Li J,Charkowick S,Felice CA,Kritzer M,Nguyen MVC,Yu P,Ballas N

更新日期：2018-06-05 00:00:00