Trifunctional lipid probes for comprehensive studies of single lipid species in living cells.

abstract：:The genomic concentrations of certain middle repetitive DNA sequences vary considerably among closely related species of Drosophila. In fact, the chromosomes of D. melanogaster appear to carry approximately 3 times as much middle repetitive DNA as those of the sibling species D. simulans. Although most of the middle r...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Dowsett AP,Young MW

更新日期：1982-08-01 00:00:00

abstract：:The protein-protein interaction (PPI) network is crucial for cellular information processing and decision-making. With suitable inputs, PPI networks drive the cells to diverse functional outcomes such as cell proliferation or cell death. Here, we characterize the structural controllability of a large directed human PP...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Vinayagam A,Gibson TE,Lee HJ,Yilmazel B,Roesel C,Hu Y,Kwon Y,Sharma A,Liu YY,Perrimon N,Barabási AL

更新日期：2016-05-03 00:00:00

abstract：:We have postulated that the toxic neuropathies associated with neurofilament-filled axonal swellings have a common pathogenesis, the covalent crosslinking of neurofilaments during anterograde transport. The newly described gamma-diketone, 3,4-dimethyl-2,5-hexanedione (DMHD), is a more potent analogue of the toxic meta...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Graham DG,Szakál-Quin G,Priest JW,Anthony DC

更新日期：1984-08-01 00:00:00

abstract：:Animal models of human cytomegalovirus (CMV) infections have not been available to study pathogenesis or to evaluate antiviral drugs. Severe combined immunodeficient mice implanted with human fetal tissues (SCID-hu) were found to support CMV replication and may provide a model for this species-specific virus. When con...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mocarski ES,Bonyhadi M,Salimi S,McCune JM,Kaneshima H

更新日期：1993-01-01 00:00:00

abstract：:Cognitive flexibility depends on a fast neural learning mechanism for enhancing momentary relevant over irrelevant information. A possible neural mechanism realizing this enhancement uses fast spiking interneurons (FSIs) in the striatum to train striatal projection neurons to gate relevant and suppress distracting cor...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Banaie Boroujeni K,Oemisch M,Hassani SA,Womelsdorf T

更新日期：2020-07-28 00:00:00

abstract：:The Sleeping Beauty (SB) transposon system can integrate foreign sequences of DNA in the genome of mouse somatic cells eliciting long-term expression in vivo. This technology holds great promise for human gene therapy as a nonviral technology to deliver therapeutic genes. SB also provides a means to study the effects ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Carlson CM,Frandsen JL,Kirchhof N,McIvor RS,Largaespada DA

更新日期：2005-11-22 00:00:00

abstract：:Some strains of Bradyrhizobium japonicum produce rhizobitoxine, a phytotoxin that causes foliar chlorosis on susceptible host plants. We have previously obtained Tn5-induced rhizobitoxine null mutants of B. japonicum. DNA sequence analysis of the region surrounding two Tn5 insertions identifies two overlapping open re...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ruan X,Zhang C,Peters NK

更新日期：1993-04-01 00:00:00

abstract：:Appropriate expression of IL-2 plays a central role during the priming and differentiation of T cells. A tight balance between IL-2 and the effector cytokine IL-17A is essential for immune homeostasis. Epigenetic mechanisms have been documented as a key component of cytokine regulation during lineage commitment. The m...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hedrich CM,Crispin JC,Rauen T,Ioannidis C,Apostolidis SA,Lo MS,Kyttaris VC,Tsokos GC

更新日期：2012-10-09 00:00:00

abstract：:The rat mammary cell line Rama 25 [Bennett, D.C., Peachey, L.A., Durbin, H. & Rudland, P.S. (1978) Cell 15, 283--298] differentiates morphologically in vitro from a cuboidal form to a fusiform cell resembling myoepithelial cells. This differentiation occurs in all clonal isolates of the line. By using three different ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lennon VA,Unger M,Dulbecco R

更新日期：1978-12-01 00:00:00

abstract：:The human rhodopsin gene is the locus for numerous alleles linked to the neurodegenerative disease retinitis pigmentosa. To facilitate the study of retinal degeneration and to test reagents designed to alter the structure and function of this gene, we have developed strains of mice whose native rhodopsin gene has been...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chan F,Bradley A,Wensel TG,Wilson JH

更新日期：2004-06-15 00:00:00

abstract：:Huntington disease (HD) is caused by an expansion mutation of the N-terminal polyglutamine of huntingtin (mHTT). mHTT is ubiquitously present, but it induces noticeable damage to the brain's striatum, thereby affecting motor, psychiatric, and cognitive functions. The striatal damage and progression of HD are associate...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sharma M,Rajendrarao S,Shahani N,Ramírez-Jarquín UN,Subramaniam S

更新日期：2020-07-07 00:00:00

abstract：:Professional antigen-presenting dendritic cells (DCs) are critical in regulating T cell immune responses at both systemic and mucosal sites. Many Lactobacillus species are normal members of the human gut microflora and most are regarded as safe when administered as probiotics. Because DCs can naturally or therapeutica...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mohamadzadeh M,Olson S,Kalina WV,Ruthel G,Demmin GL,Warfield KL,Bavari S,Klaenhammer TR

更新日期：2005-02-22 00:00:00

abstract：:Recent genome-wide association studies have revealed some genetic loci associated with serum uric acid levels and susceptibility to gout/hyperuricemia which contain potential candidates of physiologically important urate transporters. One of these novel loci is located upstream of SGK1 and SLC2A12, suggesting that var...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Toyoda Y,Takada T,Miyata H,Matsuo H,Kassai H,Nakao K,Nakatochi M,Kawamura Y,Shimizu S,Shinomiya N,Ichida K,Hosoyamada M,Aiba A,Suzuki H

更新日期：2020-08-04 00:00:00

abstract：:To investigate the role of an anchoring pocket in allele-specific peptide presentation by a major histocompatibility complex class I molecule, we "transplanted" a B pocket from HLA-A*0201 into HLA-B*2705 by site-directed mutagenesis. The resulting protein, designated B27.A2B, binds a different set of endogenous peptid...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Colbert RA,Rowland-Jones SL,McMichael AJ,Frelinger JA

更新日期：1993-07-15 00:00:00

abstract：:Two independently arising alpha heavy chain mutants have been found to synthesize heavy chains with CH1 deletions of approximately equal extent. Both were isolated from heavy chain-producing variants of the mouse myeloma W3129 and demonstrate that it is possible to arrive at the heavy chain disease phenotype by the pa...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Dackowski W,Morrison SL

更新日期：1981-11-01 00:00:00

abstract：:Although the transport sector is responsible for a large and growing share of global emissions affecting climate, its overall contribution has not been quantified. We provide a comprehensive analysis of radiative forcing from the road transport, shipping, aviation, and rail subsectors, using both past- and forward-loo...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Fuglestvedt J,Berntsen T,Myhre G,Rypdal K,Skeie RB

更新日期：2008-01-15 00:00:00

abstract：:Hox genes are conserved transcription factor-encoding genes that specify the identity of body regions in bilaterally symmetrical animals. In the cricket Gryllus bimaculatus, a member of the hemimetabolous insect group Orthoptera, the induction of a subset of mesodermal cells to form the primordial germ cells (PGCs) is...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Barnett AA,Nakamura T,Extavour CG

更新日期：2019-08-13 00:00:00

abstract：:Nuclear bodies are RNA and protein-rich, membraneless organelles that play important roles in gene regulation. The largest and most well-known nuclear body is the nucleolus, an organelle whose primary function in ribosome biogenesis makes it key for cell growth and size homeostasis. The nucleolus and other nuclear bod...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Berry J,Weber SC,Vaidya N,Haataja M,Brangwynne CP

更新日期：2015-09-22 00:00:00

abstract：:The search for ever deeper relationships among the World's languages is bedeviled by the fact that most words evolve too rapidly to preserve evidence of their ancestry beyond 5,000 to 9,000 y. On the other hand, quantitative modeling indicates that some "ultraconserved" words exist that might be used to find evidence ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Pagel M,Atkinson QD,S Calude A,Meade A

更新日期：2013-05-21 00:00:00

abstract：:In contrast to the Hox genes in arthropods and vertebrates, those in molluscs show diverse expression patterns with differences reported among lineages. Here, we investigate 2 phylogenetically distant molluscs, a gastropod and a polyplacophoran, and show that the Hox expression in both species can be divided into 2 ca...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Huan P,Wang Q,Tan S,Liu B

更新日期：2020-01-07 00:00:00

abstract：:A mutation partially suppressing the UV sensitivity caused by recF143 in a uvrA6 background was located at codon 37 of recA where GTG (valine) became ATG (methionine). This mutation, originally named srf-803, was renamed recA803. Little if any suppression of the recF143 defect in UV induction of a lexA regulon promote...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Madiraju MV,Templin A,Clark AJ

更新日期：1988-09-01 00:00:00

abstract：:The heavy chains of both botulinum neurotoxin type B and tetanus toxin form channels in planar bilayer membranes. These channels have pH-dependent and voltage-dependent properties that are remarkably similar to those previously described for diphtheria toxin. Selectivity experiments with anions and cations show that t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hoch DH,Romero-Mira M,Ehrlich BE,Finkelstein A,DasGupta BR,Simpson LL

更新日期：1985-03-01 00:00:00

abstract：:Native cytochrome c (cyt c) has a compact tertiary structure with a hexacoordinated heme iron and functions in electron transport in mitochondria and apoptosis in the cytoplasm. However, the possibility that protein modifications confer additional functions to cyt c has not been explored. Disruption of methionine 80 (...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Godoy LC,Muñoz-Pinedo C,Castro L,Cardaci S,Schonhoff CM,King M,Tórtora V,Marín M,Miao Q,Jiang JF,Kapralov A,Jemmerson R,Silkstone GG,Patel JN,Evans JE,Wilson MT,Green DR,Kagan VE,Radi R,Mannick JB

更新日期：2009-02-24 00:00:00

abstract：:CD83 is commonly known as a specific marker for mature dendritic cells. It has been shown to be important for CD4(+) T-cell development in the thymus. However, its function in the peripheral immune system remains enigmatic. Here, we show that CD83 inhibits proliferation and production of IL-2 and IFN-γ by T cells, and...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chen L,Zhu Y,Zhang G,Gao C,Zhong W,Zhang X

更新日期：2011-11-15 00:00:00

abstract：:Fragile X syndrome (FXS) results in intellectual disability (ID) most often caused by silencing of the fragile X mental retardation 1 (FMR1) gene. The resulting absence of fragile X mental retardation protein 1 (FMRP) leads to both pre- and postsynaptic defects, yet whether the pre- and postsynaptic functions of FMRP ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Myrick LK,Deng PY,Hashimoto H,Oh YM,Cho Y,Poidevin MJ,Suhl JA,Visootsak J,Cavalli V,Jin P,Cheng X,Warren ST,Klyachko VA

更新日期：2015-01-27 00:00:00

abstract：:Osteoclasts, bone-resorptive multinucleated cells derived from hematopoietic stem cells, are associated with many bone-related diseases, such as osteoporosis. Osteoclast-targeting small-molecule inhibitors are valuable tools for studying osteoclast biology and for developing antiresorptive agents. Here, we have discov...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kawatani M,Okumura H,Honda K,Kanoh N,Muroi M,Dohmae N,Takami M,Kitagawa M,Futamura Y,Imoto M,Osada H

更新日期：2008-08-19 00:00:00

abstract：:Parathyroid hormone (PTH), the major calcium-regulating hormone, and norepinephrine (NE), the principal neurotransmitter of sympathetic nerves, regulate bone remodeling by activating distinct cell-surface G protein-coupled receptors in osteoblasts: the parathyroid hormone type 1 receptor (PTHR) and the β(2)-adrenergic...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hanyu R,Wehbi VL,Hayata T,Moriya S,Feinstein TN,Ezura Y,Nagao M,Saita Y,Hemmi H,Notomi T,Nakamoto T,Schipani E,Takeda S,Kaneko K,Kurosawa H,Karsenty G,Kronenberg HM,Vilardaga JP,Noda M

更新日期：2012-05-08 00:00:00

abstract：:Mixing genomes of different species by hybridization can disrupt species-specific genetic interactions that were adapted and fixed within each species population. Such disruption can predispose the hybrids to abnormalities and disease that decrease the overall fitness of the hybrids and is therefore named as hybrid in...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lu Y,Sandoval A,Voss S,Lai Z,Kneitz S,Boswell W,Boswell M,Savage M,Walter C,Warren W,Schartl M,Walter R

更新日期：2020-11-24 00:00:00

abstract：:Long interspersed nuclear elements 1 (L1) are active retrotransposons that reside in many species, including humans and rodents. L1 elements produce an RNA intermediate that is reverse transcribed to DNA and inserted in a new genomic location. We have tagged an active human L1 element (L1(RP)) with a gene encoding enh...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Prak ET,Dodson AW,Farkash EA,Kazazian HH Jr

更新日期：2003-02-18 00:00:00

abstract：:Rotaviruses are the single most common cause of fatal and severe childhood diarrheal illness worldwide (>125 million cases annually). Rotavirus shares structural and functional features with many viruses, such as the presence of segmented double-stranded RNA genomes selectively and tightly packed with a conserved numb...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Periz J,Celma C,Jing B,Pinkney JN,Roy P,Kapanidis AN

更新日期：2013-07-16 00:00:00