Abstract:
:Long interspersed nuclear elements 1 (L1) are active retrotransposons that reside in many species, including humans and rodents. L1 elements produce an RNA intermediate that is reverse transcribed to DNA and inserted in a new genomic location. We have tagged an active human L1 element (L1(RP)) with a gene encoding enhanced GFP (EGFP). Expression of GFP occurs only if L1-EGFP has undergone a cycle of transcription, reverse transcription, and integration into a transcriptionally permissive genomic region. We show here that L1-EGFP can undergo retrotransposition in vivo and produce fluorescence in mouse testis. The retrotransposition event characterized here has occurred at a very early stage in the development of an L1-EGFP transgenic founder mouse.
journal_name
Proc Natl Acad Sci U S Aauthors
Prak ET,Dodson AW,Farkash EA,Kazazian HH Jrdoi
10.1073/pnas.0337627100keywords:
subject
Has Abstractpub_date
2003-02-18 00:00:00pages
1832-7issue
4eissn
0027-8424issn
1091-6490pii
0337627100journal_volume
100pub_type
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