Automated prediction of protein function and detection of functional sites from structure.

abstract：:Microinjection of progesterone dissolved in paraffin oil induces the reinitiation of meiotic maturation in the Xenopus oocyte; 50% maturation is obtained when 50 nl of a 50 microM solution is microinjected into the oocyte. The kinetics of the response to microinjected progesterone are similar to the kinetics of respon...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tso J,Thibier C,Mulner O,Ozon R

更新日期：1982-09-01 00:00:00

abstract：:The development of the luminescence system in Beneckea harveyi is controlled by cyclic nucleotides at the level of transcription. In the wild type, it is repressed by exogenously added guanosine 3':5'-cyclic monophosphate and this repression is overcome by the addition of adenosine 3':5'-cyclic monophosphate. These ob...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ulitzur S,Yashphe J,Hastings JW

更新日期：1976-12-01 00:00:00

abstract：:Previously metal-ion sites have been used as structural and functional probes in seven transmembrane receptors (7TM), but as yet all the engineered sites have been inactivating. Based on presumed agonist interaction points in transmembrane III (TM-III) and -VII of the beta(2)-adrenergic receptor, in this paper we cons...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Elling CE,Thirstrup K,Holst B,Schwartz TW

更新日期：1999-10-26 00:00:00

abstract：:Experiments were performed to ascertain the degree to which the amount of amino acids might be one of the regulatory factors that control the activity of the nucleolar RNA polymerase. Assays of the enzymatic activity were done with isolated nuclei from cells incubated with low and high concentrations of amino acids. S...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Franze-Fernández MT,Pogo AO

更新日期：1971-12-01 00:00:00

abstract：:Because neurogenesis persists in the adult mammalian brain and can be regulated by physiological and pathological events, we investigated its possible involvement in the brain's response to focal cerebral ischemia. Ischemia was induced by occlusion of the middle cerebral artery in the rat for 90 min, and proliferating...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Jin K,Minami M,Lan JQ,Mao XO,Batteur S,Simon RP,Greenberg DA

更新日期：2001-04-10 00:00:00

abstract：:The three-dimensional structure of O,O',N-trimethyl-d-tubocurarine, a neuromuscular blocking agent, has been determined by x-ray crystallography. This may help to provide insight into its pharmacologic action. ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sobell HM,Sakore TD,Tavale SS,Canepa FG,Pauling P,Petcher TJ

更新日期：1972-08-01 00:00:00

abstract：:Zea mays plasma membrane intrinsic proteins (ZmPIPs) fall into two groups, ZmPIP1s and ZmPIP2s, that exhibit different water channel activities when expressed in Xenopus oocytes. ZmPIP1s are inactive, whereas ZmPIP2s induce a marked increase in the membrane osmotic water permeability coefficient, P(f). We previously s...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zelazny E,Borst JW,Muylaert M,Batoko H,Hemminga MA,Chaumont F

更新日期：2007-07-24 00:00:00

abstract：:Recent evidence indicates there is a role for small membrane vesicles, including exosomes, as vehicles for intercellular communication. Exosomes secreted by most cell types can mediate transfer of proteins, mRNAs, and microRNAs, but their role in the transmission of infectious agents is less established. Recent studie...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ramakrishnaiah V,Thumann C,Fofana I,Habersetzer F,Pan Q,de Ruiter PE,Willemsen R,Demmers JA,Stalin Raj V,Jenster G,Kwekkeboom J,Tilanus HW,Haagmans BL,Baumert TF,van der Laan LJ

更新日期：2013-08-06 00:00:00

abstract：:Adult cardiomyocytes are irreversibly postmitotic but respond to a variety of stimuli by hypertrophic growth, which is associated with an increase in cell size and protein content, organization of sarcomeres, and activation of a fetal gene program. Recently, we described a novel cardiac helicase activated by MEF2 prot...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Liu ZP,Olson EN

更新日期：2002-02-19 00:00:00

abstract：:We have found that 1 of 20 human tumors transplanted and passaged in nude mice was associated with a massive induction of endogenous murine leukemia virus (MuLV). Separation and growth of these viruses on various substrates indicated that both ecotropic and xenotropic MuLV were present in the induced mixture. Tryptic ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gautsch JW,Knowles AF,Jensen FC,Kaplan NO

更新日期：1980-04-01 00:00:00

abstract：:The sequence of the transforming region of simian sarcoma virus (SSV) has been determined by using molecularly cloned viral DNA. This region encompassed the 1.0-kilobase pair woolly monkey cell-derived insertion sequence, v-sis, and flanking simian sarcoma-associated viral (SSAV) sequences. A 675-nucleotide-long open ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Devare SG,Reddy EP,Robbins KC,Andersen PR,Tronick SR,Aaronson SA

更新日期：1982-05-01 00:00:00

abstract：:Nuclear factor of activated T cells (NFAT) proteins are Ca(2+)-regulated transcription factors that control gene expression in many cell types. NFAT proteins are heavily phosphorylated and reside in the cytoplasm of resting cells; when cells are stimulated by a rise in intracellular Ca(2+), NFAT proteins are dephospho...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sharma S,Findlay GM,Bandukwala HS,Oberdoerffer S,Baust B,Li Z,Schmidt V,Hogan PG,Sacks DB,Rao A

更新日期：2011-07-12 00:00:00

abstract：:T cells are essential for immune defenses against pathogens, such that viability of naïve T cells before antigen encounter is critical to preserve a polyclonal repertoire and prevent immunodeficiencies. The viability of naïve T cells before antigen recognition is ensured by IL-7, which drives expression of the prosurv...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Miller ML,Mashayekhi M,Chen L,Zhou P,Liu X,Michelotti M,Tramontini Gunn N,Powers S,Zhu X,Evaristo C,Alegre ML,Molinero LL

更新日期：2014-05-20 00:00:00

abstract：:Cancer patients and tumor-bearing mice possess serum antibodies that recognize antigens expressed by cancer cells at the time of diagnosis. After diagnosis, cancers progress to more aggressive stages, most often by acquiring new genetic changes that can give rise to new proteins, some of which are antigenic. However, ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Spiotto MT,Reth MA,Schreiber H

更新日期：2003-04-29 00:00:00

abstract：:A mathematical method is developed which enables us to treat exactly the process of coincidental evolution under mutation, unequal intrachromosomal crossing-over as well as ordinary crossing-over between homologous chromosomes in a finite population of the effective size N. It makes use of finite difference equations ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kimura M,Ohta T

更新日期：1979-08-01 00:00:00

abstract：:Infants' sensitivity to contingent reactivity as an indicator of intentional agency has been demonstrated by numerous referential gaze-following studies. Here we propose that variability of signal sequences in a turn-taking exchange provides an informative cue for infants to recognize interactions that may involve com...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tauzin T,Gergely G

更新日期：2019-07-30 00:00:00

abstract：:Antibody modulation of T-cell coinhibitory (e.g., CTLA-4) or costimulatory (e.g., 4-1BB) receptors promotes clinical responses to a variety of cancers. Therapeutic cancer vaccination, in contrast, has produced limited clinical benefit and no curative therapies. The E6 and E7 oncoproteins of human papilloma virus (HPV)...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bartkowiak T,Singh S,Yang G,Galvan G,Haria D,Ai M,Allison JP,Sastry KJ,Curran MA

更新日期：2015-09-22 00:00:00

abstract：:After endocytosis, transmembrane cargo reaches endosomes, where it encounters complexes dedicated to opposing functions: recycling and degradation. Microdomains containing endosomal sorting complexes required for transport (ESCRT)-0 component Hrs [hepatocyte growth factor-regulated tyrosine kinase substrate (HGRS-1) i...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Norris A,Tammineni P,Wang S,Gerdes J,Murr A,Kwan KY,Cai Q,Grant BD

更新日期：2017-01-17 00:00:00

abstract：:The Per-Arnt-Sim (PAS) domain is a ubiquitous protein module with a common three-dimensional fold involved in a wide range of regulatory and sensory functions in all domains of life. The activation of these functions is thought to involve partial unfolding of N- or C-terminal helices attached to the PAS domain. Here w...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zhao JM,Lee H,Nome RA,Majid S,Scherer NF,Hoff WD

更新日期：2006-08-01 00:00:00

abstract：:The ability of the cornea to transmit light while being mechanically resilient is directly attributable to the formation of an extracellular matrix containing orthogonal sheets of collagen fibrils. The detailed structure of the fibrils and how this structure underpins the mechanical properties and organization of the ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Holmes DF,Gilpin CJ,Baldock C,Ziese U,Koster AJ,Kadler KE

更新日期：2001-06-19 00:00:00

abstract：:Conformationally compromised oncogenic mutants of the tumor suppressor protein p53 can, in principle, be rescued by small molecules that bind the native, but not the denatured state. We describe a strategy for the rational search for such molecules. A nine-residue peptide, CDB3, which was derived from a p53 binding pr...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Friedler A,Hansson LO,Veprintsev DB,Freund SM,Rippin TM,Nikolova PV,Proctor MR,Rüdiger S,Fersht AR

更新日期：2002-01-22 00:00:00

abstract：:Gradients of extracellular signaling molecules and transcription factors are used in a variety of developmental processes, including the patterning of the Drosophila embryo, the establishment of diverse neuronal cell types in the vertebrate neural tube, and the anterior-posterior patterning of vertebrate limbs. Here, ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hong JW,Hendrix DA,Papatsenko D,Levine MS

更新日期：2008-12-23 00:00:00

abstract：:It is shown that polyphosphates are not generated in significant amounts in the phosphoglycerate kinase reaction; polyphosphate is more effective than ATP in the formation of glucose 6-P by glucokinase, but the rate with ATP may be adequate to meet the requirements of glucose metabolism; PPi is far more effective than...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wood HG,Goss NH

更新日期：1985-01-01 00:00:00

abstract：:Annual reproductive success and senescence patterns vary substantially among individuals in the wild. However, it is still seldom considered that senescence may not only affect an individual but also affect age-specific reproductive success in its offspring, generating transgenerational reproductive senescence. We use...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kroeger SB,Blumstein DT,Armitage KB,Reid JM,Martin JGA

更新日期：2020-03-03 00:00:00

abstract：:We report here the cloning, expression, and characterization of a dual-substrate, cAMP and cGMP, cyclic nucleotide phosphodiesterase (PDE) from mouse. This PDE contains the consensus sequence for a PDE catalytic domain, but shares <50% sequence identity with the catalytic domains of all other known PDEs and, therefore...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Soderling SH,Bayuga SJ,Beavo JA

更新日期：1999-06-08 00:00:00

abstract：:Visual development is thought to be completed at an early age. We suggest that the maturation of the visual brain is not homogeneous: functions with greater need for early availability, such as visuomotor control, mature earlier, and the development of other visual functions may extend well into childhood. We found si...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kovács I,Kozma P,Fehér A,Benedek G

更新日期：1999-10-12 00:00:00

abstract：:The synthesis of the covalently-closed, circular DNA form of colicinogenic factor E(1) (ColE(1)) continues in Escherichia coli cells after the addition of chloramphenicol. A large portion of the purified supercoiled ColE(1) DNA molecules made in the presence of chloramphenicol are converted to the open circular DNA fo...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Blair DG,Sherratt DJ,Clewell DB,Helinski DR

更新日期：1972-09-01 00:00:00

abstract：:Tunas are migratory fishes in offshore habitats and top predators with unique features. Despite their ecological importance and high market values, the open-ocean lifestyle of tuna, in which effective sensing systems such as color vision are required for capture of prey, has been poorly understood. To elucidate the ge...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Nakamura Y,Mori K,Saitoh K,Oshima K,Mekuchi M,Sugaya T,Shigenobu Y,Ojima N,Muta S,Fujiwara A,Yasuike M,Oohara I,Hirakawa H,Chowdhury VS,Kobayashi T,Nakajima K,Sano M,Wada T,Tashiro K,Ikeo K,Hattori M,Kuhara S,

更新日期：2013-07-02 00:00:00

abstract：:Saccharopolyspora erythraea makes erythromycin, an antibiotic commonly used in human medicine. Unusually, the erythromycin biosynthetic (ery) cluster lacks a pathway-specific regulatory gene. We isolated a transcriptional regulator of the ery biosynthetic genes from S. erythraea and found that this protein appears to ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chng C,Lum AM,Vroom JA,Kao CM

更新日期：2008-08-12 00:00:00

abstract：:Membrane-associated and periplasmic proteins of Agrobacterium tumefaciens C-58 were compared with those from avirulent (nontumrigenic) derivative strains by slab and two-dimensional gel electrophoresis. Two proteins (Per-I and Per-2), with a molecular weight of 37,500 and 37,300, respectively, were detected in the sup...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sonoki S,Kado CI

更新日期：1978-08-01 00:00:00