当前位置： SCI文献检索 > PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA期刊下所有文献 > Isolation and characterization of a dual-substrate phosphodiesterase gene family: PDE10A.

Isolation and characterization of a dual-substrate phosphodiesterase gene family: PDE10A.

Abstract：

:We report here the cloning, expression, and characterization of a dual-substrate, cAMP and cGMP, cyclic nucleotide phosphodiesterase (PDE) from mouse. This PDE contains the consensus sequence for a PDE catalytic domain, but shares <50% sequence identity with the catalytic domains of all other known PDEs and, therefore, represents a new PDE gene family, designated PDE10A. The cDNA for PDE10A is 3, 370 nt in length. It includes a full ORF, contains three in-frame stop codons upstream of the first methionine, and is predicted to encode a 779-aa enzyme. At the N terminus PDE10A has two GAF domains homologous to many signaling molecules, including PDE2, PDE5, and PDE6, which likely constitute a low-affinity binding site for cGMP. PDE10A hydrolyzes cAMP with a Km of 0.05 microM and cGMP with a Km of 3 microM. Although PDE10A has a lower Km for cAMP, the Vmax ratio (cGMP/cAMP) is 4.7. RNA distribution studies indicate that PDE10A is expressed at highest levels in testis and brain.

journal_name

Proc Natl Acad Sci U S A

journal_title

Proceedings of the National Academy of Sciences of the United States of America

authors

Soderling SH,Bayuga SJ,Beavo JA

doi

10.1073/pnas.96.12.7071

keywords:

subject

Has Abstract

pub_date

1999-06-08 00:00:00

pages

7071-6

issue

12

eissn

0027-8424

issn

1091-6490

journal_volume

96

pub_type

杂志文章

相关文献

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA文献大全
  • Profilin1 regulates PI(3,4)P2 and lamellipodin accumulation at the leading edge thus influencing motility of MDA-MB-231 cells.

    abstract：:Profilin1, a ubiquitously expressed actin-binding protein, plays a critical role in cell migration through actin cytoskeletal regulation. Given the traditional view of profilin1 as a promigratory molecule, it is difficult to reconcile observations that profilin1 is down-regulated in various invasive adenocarcinomas an...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1002309107

    authors： Bae YH,Ding Z,Das T,Wells A,Gertler F,Roy P

    更新日期：2010-12-14 00:00:00

  • Deconstruction of biomass enabled by local demixing of cosolvents at cellulose and lignin surfaces.

    abstract：:A particularly promising approach to deconstructing and fractionating lignocellulosic biomass to produce green renewable fuels and high-value chemicals pretreats the biomass with organic solvents in aqueous solution. Here, neutron scattering and molecular-dynamics simulations reveal the temperature-dependent morpholog...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1922883117

    authors： Pingali SV,Smith MD,Liu SH,Rawal TB,Pu Y,Shah R,Evans BR,Urban VS,Davison BH,Cai CM,Ragauskas AJ,O'Neill HM,Smith JC,Petridis L

    更新日期：2020-07-21 00:00:00

  • Engineering human immunodeficiency virus 1 protease heterodimers as macromolecular inhibitors of viral maturation.

    abstract：:Dimerization of human immunodeficiency virus type 1 protease (HIV-1 PR) monomers is an essential prerequisite for viral proteolytic activity and the subsequent generation of infectious virus particles. Disruption of the dimer interface inhibits this activity as does formation of heterodimers between wild-type and defe...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.93.21.11477

    authors： McPhee F,Good AC,Kuntz ID,Craik CS

    更新日期：1996-10-15 00:00:00

  • Cancer vaccines: the interleukin 2 dosage effect.

    abstract：:Cancer vaccines genetically engineered to produce interleukin 2 have been investigated intensively in a series of animal models and are at the point of entering into clinical trials. In this study we demonstrate a strong correlation between the rate of interleukin 2 production and the protection efficiency of murine S...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.92.10.4711

    authors： Schmidt W,Schweighoffer T,Herbst E,Maass G,Berger M,Schilcher F,Schaffner G,Birnstiel ML

    更新日期：1995-05-09 00:00:00

  • Hydroxyl radical "footprinting" of RNA: application to pre-mRNA splicing complexes.

    abstract：:We present an adaptation of the hydroxyl radical DNA "footprinting" technique that permits high-resolution mapping of protected regions of RNA. Hydroxyl radical cleaves RNA independently of base sequence and secondary structure of the RNAs examined and allows resolution of protected regions at the single nucleotide le...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.86.20.7795

    authors： Wang XD,Padgett RA

    更新日期：1989-10-01 00:00:00

  • Effects of methylation on a synthetic polynucleotide: the B--Z transition in poly(dG-m5dC).poly(dG-m5dC).

    abstract：:We have compared the behavior in solution of the synthetic polynucleotide poly(dG-m5dC).poly(dG-m5dC) with that of the unmethylated polynucleotide poly(dG-dC).poly(dG-dC). In solutions containing high concentrations of salt, poly(dG-dC).poly(dG-dC) is known to exhibit altered circular dichroic and absorption spectra c...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.78.3.1619

    authors： Behe M,Felsenfeld G

    更新日期：1981-03-01 00:00:00

  • Development and stability of postional information in Xenopus retinal ganglion cells.

    abstract：:Neuronal specificity in retinal ganglion cells of Xenopus subserves the orderly connections of the optic nerve fibers in the tectum. This specificity derives from positional information acquired by the developing retina at embryonic stages 28-31. Here we report that ganglion cells of embryonic stage 28 eyes can acquir...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.69.4.780

    authors： Hunt RK,Jacobson M

    更新日期：1972-04-01 00:00:00

  • When defense backfires: detrimental effect of a plant's protective trichomes on an insect beneficial to the plant.

    abstract：:The plant Mentzelia pumila (family Loasaceae) has leaves and stems densely covered with tiny hooked trichomes. The structures entrap and kill insects and therefore are most probably protective. But they are also maladaptive in that they incapacitate a coccinellid beetle (Hippodamia convergens) that preys upon an aphid...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.95.8.4410

    authors： Eisner T,Eisner M,Hoebeke ER

    更新日期：1998-04-14 00:00:00

  • Exploring the origins of binding specificity through the computational redesign of calmodulin.

    abstract：:Calmodulin (CaM) is a second messenger protein that has evolved to bind tightly to a variety of targets and, as such, exhibits low binding specificity. We redesigned CaM by using a computational protein design algorithm to improve its binding specificity for one of its targets, smooth muscle myosin light chain kinase ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.2234277100

    authors： Shifman JM,Mayo SL

    更新日期：2003-11-11 00:00:00

  • NTRC-dependent redox balance of 2-Cys peroxiredoxins is needed for optimal function of the photosynthetic apparatus.

    abstract：:Thiol-dependent redox regulation allows the rapid adaptation of chloroplast function to unpredictable changes in light intensity. Traditionally, it has been considered that chloroplast redox regulation relies on photosynthetically reduced ferredoxin (Fd), thioredoxins (Trxs), and an Fd-dependent Trx reductase (FTR), t...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1706003114

    authors： Pérez-Ruiz JM,Naranjo B,Ojeda V,Guinea M,Cejudo FJ

    更新日期：2017-11-07 00:00:00

  • Sphingosine-1-phosphate receptor 1 activation in astrocytes contributes to neuropathic pain.

    abstract：:Neuropathic pain afflicts millions of individuals and represents a major health problem for which there is limited effective and safe therapy. Emerging literature links altered sphingolipid metabolism to nociceptive processing. However, the neuropharmacology of sphingolipid signaling in the central nervous system in t...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1820466116

    authors： Chen Z,Doyle TM,Luongo L,Largent-Milnes TM,Giancotti LA,Kolar G,Squillace S,Boccella S,Walker JK,Pendleton A,Spiegel S,Neumann WL,Vanderah TW,Salvemini D

    更新日期：2019-05-21 00:00:00

  • Evolutionary analyses of hedgehog and Hoxd-10 genes in fish species closely related to the zebrafish.

    abstract：:The study of development has relied primarily on the isolation of mutations in genes with specific functions in development and on the comparison of their expression patterns in normal and mutant phenotypes. Comparative evolutionary analyses can complement these approaches. Phylogenetic analyses of Sonic hedgehog (Shh...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.93.23.13036

    authors： Zardoya R,Abouheif E,Meyer A

    更新日期：1996-11-12 00:00:00

  • Endothelin 1, an endothelium-derived peptide, is expressed in neurons of the human spinal cord and dorsal root ganglia.

    abstract：:The localization of endothelin 1 mRNA and endothelin-like immunoreactivity was investigated in samples of neurologically normal nervous system tissue from 10 adults by using in situ hybridization and immunocytochemistry. Tissue sections of spinal cord and dorsal root ganglia were hybridized with an 35S-radiolabeled en...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.86.19.7634

    authors： Giaid A,Gibson SJ,Ibrahim BN,Legon S,Bloom SR,Yanagisawa M,Masaki T,Varndell IM,Polak JM

    更新日期：1989-10-01 00:00:00

  • Resolution of single and double Holliday junction recombination intermediates by GEN1.

    abstract：:Genetic recombination provides an important mechanism for the repair of DNA double-strand breaks. Homologous pairing and strand exchange lead to the formation of DNA intermediates, in which sister chromatids or homologous chromosomes are covalently linked by four-way Holliday junctions (HJs). Depending on the type of ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1619790114

    authors： Shah Punatar R,Martin MJ,Wyatt HD,Chan YW,West SC

    更新日期：2017-01-17 00:00:00

  • Tumor-initiating stem cells of squamous cell carcinomas and their control by TGF-β and integrin/focal adhesion kinase (FAK) signaling.

    abstract：:Cancer stem cells (CSCs) sustain tumor growth through their ability to self-renew and to generate differentiated progeny. These functions endow CSCs with the potential to initiate secondary tumors bearing characteristics similar to those of the parent. Recently the hair follicle stem cell marker CD34 was used to purif...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1107807108

    authors： Schober M,Fuchs E

    更新日期：2011-06-28 00:00:00

  • CXCR4-CCR5: a couple modulating T cell functions.

    abstract：:Chemokines and their receptors direct leukocyte migration among blood, lymph and tissues. Evidence has recently accumulated that, besides their chemotactic functions, chemokine receptors are highly versatile players that fine tune immune responses. During human T cell activation by antigen-presenting cells, the chemok...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0804286105

    authors： Contento RL,Molon B,Boularan C,Pozzan T,Manes S,Marullo S,Viola A

    更新日期：2008-07-22 00:00:00

  • STRUBBELIG defines a receptor kinase-mediated signaling pathway regulating organ development in Arabidopsis.

    abstract：:An open question remains as to what coordinates cell behavior during organogenesis, permitting organs to reach their appropriate size and shape. The Arabidopsis gene STRUBBELIG (SUB) defines a receptor-mediated signaling pathway in plants. SUB encodes a putative leucine-rich repeat transmembrane receptor-like kinase. ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0503526102

    authors： Chevalier D,Batoux M,Fulton L,Pfister K,Yadav RK,Schellenberg M,Schneitz K

    更新日期：2005-06-21 00:00:00

  • Personality predicts activity in reward and emotional regions associated with humor.

    abstract：:Previous research and theory suggest that two stable personality dimensions, extroversion and neuroticism, differentially influence emotional reactivity to a variety of pleasurable phenomena. Here, we use event-related functional MRI to address the putative neural and behavioral associations between humor appreciation...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0408457102

    authors： Mobbs D,Hagan CC,Azim E,Menon V,Reiss AL

    更新日期：2005-11-08 00:00:00

  • Fibroblast cell interactions with human melanoma cells affect tumor cell growth as a function of tumor progression.

    abstract：:It is known from a variety of experimental systems that the ability of tumor cells to grow locally and metastasize can be affected by the presence of adjacent normal tissues and cells, particularly mesenchymally derived stromal cells such as fibroblasts. However, the comparative influence of such normal cell-tumor cel...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.88.14.6028

    authors： Cornil I,Theodorescu D,Man S,Herlyn M,Jambrosic J,Kerbel RS

    更新日期：1991-07-15 00:00:00

  • Maladaptive DNA repair is the ultimate contributor to the death of trimethoprim-treated cells under aerobic and anaerobic conditions.

    abstract：:The bactericidal effects of antibiotics are undoubtedly triggered by target-specific interactions, but there is growing evidence that an important aspect of cytotoxicity results from treatment-induced metabolic perturbations. In this study, we characterized molecular mechanisms whereby trimethoprim treatment results i...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1706236114

    authors： Giroux X,Su WL,Bredeche MF,Matic I

    更新日期：2017-10-24 00:00:00

  • Rapid evolutionary radiation of marine zooplankton in peripheral environments.

    abstract：:Populations of jellyfish, Mastigias sp., landlocked in tropical marine lakes during the Holocene, show extreme genetic isolation (0.74 < or = phiST < or = 1.00), founder effects (genetic diversity: 0.000 < or = pi < or = 0.001), rapid morphological evolution, and behavioral adaptation. These results demonstrate incipi...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0503635102

    authors： Dawson MN,Hamner WM

    更新日期：2005-06-28 00:00:00

  • Pelizaeus-Merzbacher disease: a valine to phenylalanine point mutation in a putative extracellular loop of myelin proteolipid.

    abstract：:In the central nervous system, myelin proteolipid protein isoforms (PLP and DM20) play an essential structural role in myelination. It has been shown in several species that myelination is impaired by molecular defects resulting from single base mutations in the PLP gene. We have used DNA amplification by polymerase c...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.88.17.7562

    authors： Pham-Dinh D,Popot JL,Boespflug-Tanguy O,Landrieu P,Deleuze JF,Boué J,Jollès P,Dautigny A

    更新日期：1991-09-01 00:00:00

  • Subversion of B lymphocyte tolerance by hydralazine, a potential mechanism for drug-induced lupus.

    abstract：:Accumulating evidence indicates that epigenetic alterations contribute to exacerbated activation or deregulation of the mechanisms that maintain tolerance to self-antigens in patients with lupus, a systemic autoimmune disease that can be triggered by medications taken to treat a variety of conditions. Here, we tested ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0610434104

    authors： Mazari L,Ouarzane M,Zouali M

    更新日期：2007-04-10 00:00:00

  • A molecular profile of the mouse gastric parietal cell with and without exposure to Helicobacter pylori.

    abstract：:The parietal cell (PC) plays an important role in normal gastric physiology and in common diseases of the stomach. Although the genes involved in acid secretion are well known, there is limited molecular information about other aspects of PC function. We have generated a comprehensive database of genes expressed prefe...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.231332398

    authors： Mills JC,Syder AJ,Hong CV,Guruge JL,Raaii F,Gordon JI

    更新日期：2001-11-20 00:00:00

  • Contingent gene regulatory networks and B cell fate specification.

    abstract：:The B cell developmental pathway represents a leading system for the analysis of regulatory circuits that orchestrate cell fate specification and commitment. Considerable progress has been achieved within the past decade in the identification and genetic analysis of various regulatory components. These components incl...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章,评审

    doi：10.1073/pnas.0500480102

    authors： Singh H,Medina KL,Pongubala JM

    更新日期：2005-04-05 00:00:00

  • Circadian clock-controlled genes isolated from Neurospora crassa are late night- to early morning-specific.

    abstract：:An endogenous circadian biological clock controls the temporal aspects of life in most organisms, including rhythmic control of genes involved in clock output pathways. In the fungus Neurospora crassa, one pathway known to be under control of the clock is asexual spore (conidia) development. To understand more fully t...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.93.23.13096

    authors： Bell-Pedersen D,Shinohara ML,Loros JJ,Dunlap JC

    更新日期：1996-11-12 00:00:00

  • Self-amplifying autocrine actions of BDNF in axon development.

    abstract：:A critical step in neuronal development is the formation of axon/dendrite polarity, a process involving symmetry breaking in the newborn neuron. Local self-amplifying processes could enhance and stabilize the initial asymmetry in the distribution of axon/dendrite determinants, but the identity of these processes remai...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1115907108

    authors： Cheng PL,Song AH,Wong YH,Wang S,Zhang X,Poo MM

    更新日期：2011-11-08 00:00:00

  • Structure of the full-length Shaker potassium channel Kv1.2 by normal-mode-based X-ray crystallographic refinement.

    abstract：:Voltage-dependent potassium channels (Kv) are homotetramers composed of four voltage sensors and one pore domain. Because of high-level structural flexibility, the first mammalian Kv structure, Kv1.2 at 2.9 A, has about 37% molecular mass of the transmembrane portion not resolved. In this study, by applying a novel no...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1000142107

    authors： Chen X,Wang Q,Ni F,Ma J

    更新日期：2010-06-22 00:00:00

  • Identification of a receptor required for the anti-inflammatory activity of IVIG.

    abstract：:The anti-inflammatory activity of intravenous Ig (IVIG) results from a minor population of the pooled IgG molecules that contains terminal alpha2,6-sialic acid linkages on their Fc-linked glycans. These anti-inflammatory properties can be recapitulated with a fully recombinant preparation of appropriately sialylated I...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0810163105

    authors： Anthony RM,Wermeling F,Karlsson MC,Ravetch JV

    更新日期：2008-12-16 00:00:00

  • Understanding the mechanical response of double-stranded DNA and RNA under constant stretching forces using all-atom molecular dynamics.

    abstract：:Multiple biological processes involve the stretching of nucleic acids (NAs). Stretching forces induce local changes in the molecule structure, inhibiting or promoting the binding of proteins, which ultimately affects their functionality. Understanding how a force induces changes in the structure of NAs at the atomic l...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1705642114

    authors： Marin-Gonzalez A,Vilhena JG,Perez R,Moreno-Herrero F

    更新日期：2017-07-03 00:00:00