FRET imaging in living maize cells reveals that plasma membrane aquaporins interact to regulate their subcellular localization.

abstract：:Like all aliphatic amines, the polyamines spermine and spermidine are physical quenchers of singlet molecular oxygen (1O2*). The rate constants of these processes were determined in vitro with photochemically generated 1O2* and the hydrocarbon rubrene as substrate, in pyridine. At millimolar concentration, spermine an...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Khan AU,Mei YH,Wilson T

更新日期：1992-12-01 00:00:00

abstract：:Physiological and evolutionary adaptations operate at very different time scales. Nevertheless, there are reasons to believe there should be a strong relationship between the two, as together they modify the phenotype. Physiological adaptations change phenotype by altering certain microscopic parameters; evolutionary ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Milo R,Hou JH,Springer M,Brenner MP,Kirschner MW

更新日期：2007-10-23 00:00:00

abstract：:Factor VII is a precursor to a serine protease that is present in mammalian plasma. In its activated form, it participates in blood coagulation by activating factor X and/or factor IX in the presence of tissue factor and calcium. Clones coding for factor VII were obtained from two cDNA libraries prepared from poly(A) ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hagen FS,Gray CL,O'Hara P,Grant FJ,Saari GC,Woodbury RG,Hart CE,Insley M,Kisiel W,Kurachi K

更新日期：1986-04-01 00:00:00

abstract：:Rapid and efficient protocols to generate oligodendrocytes (OL) from human induced pluripotent stem cells (iPSC) are currently lacking, but may be a key technology to understand the biology of myelin diseases and to develop treatments for such disorders. Here, we demonstrate that the induction of three transcription f...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ehrlich M,Mozafari S,Glatza M,Starost L,Velychko S,Hallmann AL,Cui QL,Schambach A,Kim KP,Bachelin C,Marteyn A,Hargus G,Johnson RM,Antel J,Sterneckert J,Zaehres H,Schöler HR,Baron-Van Evercooren A,Kuhlmann T

更新日期：2017-03-14 00:00:00

abstract：:The phytochrome family of informational photoreceptors has a central role in regulating light-responsive gene expression, but the mechanism of intracellular signal transduction has remained elusive. In a genetic screen for T DNA-tagged Arabidopsis mutants affected in early signaling intermediates, we identified poc1 (...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Halliday KJ,Hudson M,Ni M,Qin M,Quail PH

更新日期：1999-05-11 00:00:00

abstract：:The apoprotein corresponding to the mammalian photoreceptor rhodopsin has been expressed by using suspension cultures of HEK293S cells in defined media that contained 6-15N-lysine and 2-13C-glycine. Typical yields were 1.5-1.8 mg/liter. Incorporation of 6-15N-lysine was quantitative, whereas that of 2-13C-glycine was ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Eilers M,Reeves PJ,Ying W,Khorana HG,Smith SO

更新日期：1999-01-19 00:00:00

abstract：:Differentiation-inducing factor 1 [DIF-1; 1-(3,5-dichloro-2,6-dihydroxy-4-methoxyphenyl)-hexan-1-one] induces stalk cell differentiation during Dictyostelium development. It is present as a gradient in the multicellular slug, its lowest concentration being in the anterior. Here we demonstrate the existence of a locali...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kay RR,Large S,Traynor D,Nayler O

更新日期：1993-01-15 00:00:00

abstract：:Viral shells are self-assembled protein nanocontainers with remarkable material properties. They combine simplicity of construction with toughness and complex functionality. These properties make them interesting for bionanotechnology. To date we know little about how virus structure determines assembly pathways and s...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ivanovska IL,Miranda R,Carrascosa JL,Wuite GJ,Schmidt CF

更新日期：2011-08-02 00:00:00

abstract：:Sensitive optical signal detection can often be confounded by the presence of a significant background, and, as such, predetection background suppression is substantively important for weak signal detection. In this paper, we present a novel optical structure design, termed surface-wave-enabled darkfield aperture (SWE...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zheng G,Cui X,Yang C

更新日期：2010-05-18 00:00:00

abstract：:Transcription initiation factor TFIID is a multimeric protein complex that plays a central role in transcriptional regulation by facilitating promoter responses to various activators. cDNAs encoding the 110-kDa subunit of Drosophila TFIID (p110) were isolated with a degenerate oligodeoxynucleotide probe based on an am...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kokubo T,Gong DW,Roeder RG,Horikoshi M,Nakatani Y

更新日期：1993-07-01 00:00:00

abstract：:Green fluorescent protein (GFP) is widely used as a reporter gene in both prokaryotes and eukaryotes. However, the fluorescence levels of wild-type GFP (wtGFP) are not bright enough for fluorescence-activated cell sorting or flow cytometry. Several GFP variants were generated that are brighter or have altered excitati...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Anderson MT,Tjioe IM,Lorincz MC,Parks DR,Herzenberg LA,Nolan GP,Herzenberg LA

更新日期：1996-08-06 00:00:00

abstract：:The nuclear factor-κB (NFκB) family of transcription factors has been implicated in inflammatory disorders, viral infections, and cancer. Most of the drugs that inhibit NFκB show significant side effects, possibly due to sustained NFκB suppression. Drugs affecting induced, but not basal, NFκB activity may have the pot...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chen M,Liang J,Ji H,Yang Z,Altilia S,Hu B,Schronce A,McDermott MSJ,Schools GP,Lim CU,Oliver D,Shtutman MS,Lu T,Stark GR,Porter DC,Broude EV,Roninson IB

更新日期：2017-09-19 00:00:00

abstract：:Although ghrelin has been demonstrated to stimulate energy intake and storage through a central mechanism, its effect on hepatic lipid metabolism remains largely uncharacterized. Ghrelin receptor antagonism or gene deletion significantly decreased obesity-associated hepatic steatosis by suppression of de novo lipogene...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Li Z,Xu G,Qin Y,Zhang C,Tang H,Yin Y,Xiang X,Li Y,Zhao J,Mulholland M,Zhang W

更新日期：2014-09-09 00:00:00

abstract：:Targeting and innervation of the cerebral cortex by thalamic afferents is a key event in the specification of cortical areas. The molecular targets of thalamic regulation, however, have remained elusive. We now demonstrate that thalamic afferents regulate the expression of gamma-aminobutyric acid type A (GABAA) recept...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Paysan J,Kossel A,Bolz J,Fritschy JM

更新日期：1997-06-24 00:00:00

abstract：:Autism spectrum disorder (ASD) is characterized by impaired social interactions and communication. The pathogenesis of ASD is not known, but it involves activation of microglia. We had shown that the peptide neurotensin (NT) is increased in the serum of children with ASD and stimulates cultured adult human microglia t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tsilioni I,Pantazopoulos H,Conti P,Leeman SE,Theoharides TC

更新日期：2020-07-14 00:00:00

abstract：:The structural gene for plasmid-mediated ampicillin resistance resides upon a 3.2 X 10(6) dalton transposable sequence (TnA) flanked by short inverted repeated sequences that accompany its insertion. TnA was transposed to pMB8, a 1.8 X 10(6) dalton derivative of the colicingenic plasmid ColE1. Random deletions were in...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Heffron F,Bedinger P,Champoux JJ,Falkow S

更新日期：1977-02-01 00:00:00

abstract：:Thioredoxins are small multifunctional redox active proteins widely if not universally distributed among living organisms. In chloroplasts, two types of thioredoxins (f and m) coexist and play central roles in regulating enzyme activity. Reduction of thioredoxins in chloroplasts is catalyzed by an iron-sulfur disulfid...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Balmer Y,Koller A,del Val G,Manieri W,Schürmann P,Buchanan BB

更新日期：2003-01-07 00:00:00

abstract：:The proliferating cell nuclear antigen (PCNA) acts as a processivity factor for replicative DNA polymerases and is essential for DNA replication. In vitro studies have suggested a role for PCNA-in the repair synthesis step of nucleotide excision repair, and PCNA interacts with the cyclin-dependent kinase inhibitor p21...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Torres-Ramos CA,Yoder BL,Burgers PM,Prakash S,Prakash L

更新日期：1996-09-03 00:00:00

abstract：:RNA interference (RNAi) is being used to develop methods to control pests and disease vectors. RNAi is robust and systemic in coleopteran insects but is quite variable in other insects. The determinants of efficient RNAi in coleopterans, as well as its potential mechanisms of resistance, are not known. RNAi screen ide...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yoon JS,Mogilicherla K,Gurusamy D,Chen X,Chereddy SCRR,Palli SR

更新日期：2018-08-14 00:00:00

abstract：:Splenic lymphocytes from a Lewis rat, immunized with purified estradiol-receptor complex of calf uterine nuclei, were fused with cells of three different mouse myeloma lines (P3-X63-Ag8, P3-NSI/I-Ag4-1, and Sp2/0-Ag14) to yield hybridoma cultures, 9% of which produced antibodies to the receptor protein (estrophilin). ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Greene GL,Fitch FW,Jensen EV

更新日期：1980-01-01 00:00:00

abstract：:The present studies were conducted to determine whether prostaglandin F2 alpha (PGF2 alpha) stimulates the production of "second messengers" derived from inositol phospholipid hydrolysis and increases intracellular free Ca2+ ([Ca2+]i) in isolated bovine luteal cells. PGF2 alpha provoked rapid (10 sec) and sustained (u...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Davis JS,Weakland LL,Weiland DA,Farese RV,West LA

更新日期：1987-06-01 00:00:00

abstract：:Splice variants (SVs) of receptors for growth hormone-releasing hormone (GHRH) have been found in primary human prostate cancers and diverse human cancer cell lines. GHRH antagonists inhibit growth of various experimental human cancers, including pancreatic and colorectal, xenografted into nude mice or cultured in vit...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Busto R,Schally AV,Varga JL,Garcia-Fernandez MO,Groot K,Armatis P,Szepeshazi K

更新日期：2002-09-03 00:00:00

abstract：:Chromatin becomes reorganized during mitosis each cell cycle. To identify genes potentially involved in these supramolecular events, we have used a colony-color assay to screen temperature-sensitive mutants of Saccharomyces cerevisiae. When a sequence that mediates attachment to the nuclear matrix in vitro was inserte...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Fishel BR,Sperry AO,Garrard WT

更新日期：1993-06-15 00:00:00

abstract：:cDNA coding for protein C has been cloned from a bovine liver library in plasmid vector pBR322 and its sequence has been determined. Two overlapping clones code for the entire light and heavy chains of the mature protein, as well as a previously unreported connecting dipeptide (Lys-Arg) and a 39-amino acid leader pept...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Long GL,Belagaje RM,MacGillivray RT

更新日期：1984-09-01 00:00:00

abstract：:Lizards and snakes putatively arose between the early Jurassic and late Triassic; they diversified worldwide and now occupy many different ecological niches, making them ideal for testing theories on the origin of ecological traits. We propose and test the "deep history hypothesis," which claims that differences in ec...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Vitt LJ,Pianka ER

更新日期：2005-05-31 00:00:00

abstract：:Litter size and mammary number in the mammalian order Rodentia show a significant positive correlation. Mean litter size is typically one-half the number of available mammaries, while maximum litter size approximates mammary number. Similar relationships are found in the families Muridae, Cricetidae, and Sciuridae. Th...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gilbert AN

更新日期：1986-07-01 00:00:00

abstract：:Kaposi's sarcoma-associated herpesvirus (KSHV), etiologically associated with Kaposi's sarcoma, uses integrins (α3β1, αVβ3, and αVβ5) and associated signaling to enter human dermal microvascular endothelial cells (HMVEC-d), an in vivo target of infection. KSHV infection activated c-Cbl, which induced the selective tra...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chakraborty S,Veettil MV,Bottero V,Chandran B

更新日期：2012-05-08 00:00:00

abstract：:The NIFA protein of Klebsiella pneumoniae is required for transcription of all nif (nitrogen fixation) operons except the regulatory nifLA operon itself. NIFA activates transcription of nif operons by the alternative holoenzyme form of RNA polymerase, sigma 54-holoenzyme, in a nucleoside triphosphate (NTP)-dependent m...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Berger DK,Narberhaus F,Kustu S

更新日期：1994-01-04 00:00:00

abstract：:Serotonin (5-hydroxytryptamine; 0.5 microM and above) stimulated the synthesis of prostacyclin (as measured by radioimmunoassay of 6-ketoprostaglandin F1 alpha) by bovine aortic smooth muscle cells in culture. This effect was structurally specific; a similar response was not elicited by the other indoles (tryptophan, ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Coughlin SR,Moskowitz MA,Antoniades HN,Levine L

更新日期：1981-11-01 00:00:00

abstract：:Population outbreaks in tundra rodents have intrigued scientists for a century as a result of their spectacular appearances and their general lessons in ecology. One outstanding question that has led to competing hypotheses is why sympatric lemmings and voles differ in regularity and shape of their outbreaks. Lemming ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ims RA,Yoccoz NG,Killengreen ST

更新日期：2011-02-01 00:00:00