Inactivation of the mouse sperm receptor, mZP3, by site-directed mutagenesis of individual serine residues located at the combining site for sperm.

abstract：:Lakes are considered the second largest natural source of atmospheric methane (CH4). However, current estimates are still uncertain and do not account for diel variability of CH4 emissions. In this study, we performed high-resolution measurements of CH4 flux from several lakes, using an automated and sensor-based flux...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sieczko AK,Duc NT,Schenk J,Pajala G,Rudberg D,Sawakuchi HO,Bastviken D

更新日期：2020-09-01 00:00:00

abstract：:Antigen stimulation of mast cells via FcepsilonRI, the high-affinity receptor for IgE, triggers a signaling cascade that requires Ca(2+) mobilization for exocytosis of secretory granules during an allergic response. This study investigates critical signaling components by using mutant RBL mast cells that are defective...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hong-Geller E,Holowka D,Siraganian RP,Baird B,Cerione RA

更新日期：2001-01-30 00:00:00

abstract：:Using data from the Fragile Families and Child Wellbeing Study, this study examined the effects of the Great Recession on maternal harsh parenting. We found that changes in macroeconomic conditions, rather than current conditions, affected harsh parenting, that declines in macroeconomic conditions had a stronger impac...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lee D,Brooks-Gunn J,McLanahan SS,Notterman D,Garfinkel I

更新日期：2013-08-20 00:00:00

abstract：:Vesicles were constituted with glycophorin, the Sendai virus receptor of human erythrocytes, and loaded with calcein, a polar derivative of fluorescein, at self-quenching concentrations. On exposure to Sendai virus and mild hypo-osmotic stress, vesicles of the appropriate composition released a significant portion of ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kundrot CE,Spangler EA,Kendall DA,MacDonald RC,MacDonald RI

更新日期：1983-03-01 00:00:00

abstract：:The concept of contagion has steadily expanded from its original grounding in epidemic disease to describe a vast array of processes that spread across networks, notably social phenomena such as fads, political opinions, the adoption of new technologies, and financial decisions. Traditional models of social contagion ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ugander J,Backstrom L,Marlow C,Kleinberg J

更新日期：2012-04-17 00:00:00

abstract：:Dictyostelium actin was shown to become phosphorylated on Tyr-53 late in the developmental cycle and when cells in the amoeboid stage are subjected to stress but the phosphorylated actin had not been purified and characterized. We have separated phosphorylated and unphosphorylated actin and shown that Tyr-53 phosphory...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Liu X,Shu S,Hong MS,Levine RL,Korn ED

更新日期：2006-09-12 00:00:00

abstract：:Tadpole collagenase (EC 3.4.24.3) cleaved chick cranial bone procollagen into two triple-stranded fragments, PCA and PCB. Only PCB, with an estimated molecular weight of about 60,000 for each component chain after reduction, was found to contain interchain disulfide bonds. The analogous cleavage of collagen is known t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Byers PH,Click EM,Harper E,Bornstein P

更新日期：1975-08-01 00:00:00

abstract：:Calmodulin, a wide-spread eukaryotic Ca2+-binding protein, was phosphorylated at its tyrosine residues in Rous sarcoma virus (RSV)-transformed chicken and rat cells but not in normal chicken embryo fibroblasts. In contrast, serine and threonine phosphorylation of calmodulin was found to occur in both normal and virus-...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Fukami Y,Nakamura T,Nakayama A,Kanehisa T

更新日期：1986-06-01 00:00:00

abstract：:Sera from 11 individuals which were classified as Lp(a-) by direct gel diffusion and by absorption tests showed the presence of Lp(a) lipoprotein when the sera were concentrated 120-fold. This Lp(a) antigen was demonstrated by gel diffusion to be indistinguishable from Lp-lipoprotein isolated from known Lp(a+) sera. T...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Harvie NR,Schultz JS

更新日期：1970-05-01 00:00:00

abstract：:Many pathological conditions of the central nervous system involve damage to and removal of myelin membrane. Very little is known about initiation of this membrane damage and the mechanisms of disposal of the damaged tissue. We are interested in the interaction between complement (the components of complement are desi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Vanguri P,Koski CL,Silverman B,Shin ML

更新日期：1982-05-01 00:00:00

abstract：:Ice-core records show that climate changes in the past have been large, rapid, and synchronous over broad areas extending into low latitudes, with less variability over historical times. These ice-core records come from high mountain glaciers and the polar regions, including small ice caps and the large ice sheets of ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Alley RB

更新日期：2000-02-15 00:00:00

abstract：:The type I collagenopathies are a group of heterogeneous connective tissue disorders, that are caused by mutations in the genes encoding type I collagen and include specific forms of osteogenesis imperfecta (OI) and the Ehlers-Danlos syndrome (EDS). These disorders present with a broad disease spectrum and large clini...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gistelinck C,Kwon RY,Malfait F,Symoens S,Harris MP,Henke K,Hawkins MB,Fisher S,Sips P,Guillemyn B,Bek JW,Vermassen P,De Saffel H,Witten PE,Weis M,De Paepe A,Eyre DR,Willaert A,Coucke PJ

更新日期：2018-08-21 00:00:00

abstract：:Purified fractions of human apotransferrin, monoferric transferrins with iron on the acid-labile binding site and on the acid-stable binding site, and diferric transferrin have been prepared. The iron loading and unloading behavior of these preparations has been examined by isoelectric focusing. Iron release from the ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Huebers H,Josephson B,Huebers E,Csiba E,Finch C

更新日期：1981-04-01 00:00:00

abstract：:Two-dimensional 1H NMR investigations were used to locate elements of regular secondary structure in the human complement protein C3a (the des-Arg77 derivative) in solution. The results were compared to a refined crystal structure based on the 3.2-A resolution structure of des-Arg77-C3a [Huber, R., Scholze, H., Paques...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Nettesheim DG,Edalji RP,Mollison KW,Greer J,Zuiderweg ER

更新日期：1988-07-01 00:00:00

abstract：:The binned distribution densities of magnitudes in both the complete and the declustered catalogs of earthquakes in the Southern California region have two significantly different branches with crossover magnitude near M = 4.8. In the case of declustered earthquakes, the b-values on the two branches differ significant...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Knopoff L

更新日期：2000-10-24 00:00:00

abstract：:The attachment of human polymorphonuclear leukocytes (PMN) to bovine endothelial monolayers is increased by pretreatment of the endothelial cells with leukotriene B4 (LTB4). The response is dose dependent with a maximum at 10(-6) M, requires no more than 1 min of preincubation for the maximal effect, and decays linear...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hoover RL,Karnovsky MJ,Austen KF,Corey EJ,Lewis RA

更新日期：1984-04-01 00:00:00

abstract：:Signaling through Janus kinases (JAKs) and signal transducers and activators of transcription (STATs) is central to the responses to the majority of cytokines and some growth factors, including the interferons (IFNs) and the IL-6 family of cytokines. The biological responses to stimulation through the widely distribut...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Costa-Pereira AP,Tininini S,Strobl B,Alonzi T,Schlaak JF,Is'harc H,Gesualdo I,Newman SJ,Kerr IM,Poli V

更新日期：2002-06-11 00:00:00

abstract：:Gonadotropin-releasing hormone (GnRH) is important in reproduction, although the mechanism of central hypogonadism in humans remains unclear. Because the GnRH neuron originates from the olfactory placode and migrates to the hypothalamus during development, central hypogonadism in humans could be caused by failure in n...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Radovick S,Wray S,Lee E,Nicols DK,Nakayama Y,Weintraub BD,Westphal H,Cutler GB Jr,Wondisford FE

更新日期：1991-04-15 00:00:00

abstract：:We investigated patterns of genetic diversity of Plasmodium falciparum associated with its two main African vectors: Anopheles gambiae and Anopheles funestus. We dissected 10,296 wild-caught mosquitoes from three tropical sites, two in Cameroon (Simbock and Tibati, separated by 320 km) and one in Kenya (Rota, >2,000 k...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Annan Z,Durand P,Ayala FJ,Arnathau C,Awono-Ambene P,Simard F,Razakandrainibe FG,Koella JC,Fontenille D,Renaud F

更新日期：2007-05-08 00:00:00

abstract：:Antibodies can be developed to directly inhibit almost any protein, but their inability to enter the cytosol limits inhibitory antibodies to membrane-associated or extracellular targets. Developing a cytosolic antibody delivery system would offer unique opportunities to directly inhibit and study intracellular protein...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wang HH,Tsourkas A

更新日期：2019-10-29 00:00:00

abstract：:The effect of Zn2+ on a voltage-dependent, transient potassium current (IA) in acutely dissociated neurons from the suprachiasmatic nucleus was studied with the whole-cell patch-clamp technique. At micromolar concentrations, Zn2+ markedly potentiated IA activated from a holding potential of -60 mV, which is the restin...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Huang RC,Peng YW,Yau KW

更新日期：1993-12-15 00:00:00

abstract：:A genetic complementation system was developed in which tobacco etch virus (TEV) polymerase (NIb)-expressing transgenic plants or protoplasts were inoculated with NIb-defective TEV mutants. A beta-glucuronidase (GUS) reporter gene integrated into the genomes of parental and four mutant viruses was used to assay RNA am...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Li XH,Carrington JC

更新日期：1995-01-17 00:00:00

abstract：:The production of the staphylococcal exotoxin toxic shock syndrome toxin-1 (TSST-1) by Staphylococcus aureus has been associated with essentially all cases of menstruation-associated toxic shock syndrome (TSS). In this work, we show that the human vaginal isolate Lactobacillus reuteri RC-14 produces small signaling mo...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Li J,Wang W,Xu SX,Magarvey NA,McCormick JK

更新日期：2011-02-22 00:00:00

abstract：:The affinity and stoichiometry of DNA binding by Escherichia coli trp repressor were studied by electrophoresis in nondenaturing gels. The ability of trp repressor to retard the electrophoretic mobility of an operator DNA fragment depends on the pH of the gel system. Above the pI of the protein, little retardation of ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Carey J

更新日期：1988-02-01 00:00:00

abstract：:Remarkable progress has come from whole-brain models linking anatomy and function. Paradoxically, it is not clear how a neuronal dynamical system running in the fixed human anatomical connectome can give rise to the rich changes in the functional repertoire associated with human brain function, which is impossible to ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kringelbach ML,Cruzat J,Cabral J,Knudsen GM,Carhart-Harris R,Whybrow PC,Logothetis NK,Deco G

更新日期：2020-04-28 00:00:00

abstract：:When two phases of water are at equilibrium, the ratio of hydrogen isotopes in each is slightly altered because of their different phase affinities. This isotopic fractionation process can be utilized to analyze water's movement in the world's climate. Here we show that equilibrium fractionation ratios, an entirely qu...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Markland TE,Berne BJ

更新日期：2012-05-22 00:00:00

abstract：:The conformation of L-3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35) has been derived from electron-density maps calculated at 2.8-A resolution with phases obtained from two heavy-atom derivatives and the bound coenzyme, NAD. Like other dehydrogenases, 3-hydroxyacyl-CoA dehydrogenase is a double-domain structure, but t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Birktoft JJ,Holden HM,Hamlin R,Xuong NH,Banaszak LJ

更新日期：1987-12-01 00:00:00

abstract：:Due to intrinsic aggressiveness and lack of effective therapies, prognosis of pancreatic cancer remains dismal. Because the only molecular targeted drug approved for pancreatic ductal adenocarcinoma is a kinase inhibitor specific to the epidermal growth factor receptor (EGFR), and this receptor collaborates with anoth...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Maron R,Schechter B,Mancini M,Mahlknecht G,Yarden Y,Sela M

更新日期：2013-09-17 00:00:00

abstract：:Major histocompatibility complex (MHC) class II molecules are heterodimeric glycoproteins with one alpha and one beta polypeptide chain of similar molecular size. In this report, we describe the binding of an acetylated N-terminal peptide of myelin basic protein, [Ala4]MBP-(1-14), to purified individual alpha and beta...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Nag B,Wada HG,Deshpande SV,Passmore D,Kendrick T,Sharma SD,Clark BR,McConnell HM

更新日期：1993-02-15 00:00:00

abstract：:Entorhinal grid cells integrate sensory and self-motion inputs to provide a spatial metric of a characteristic scale. One function of this metric may be to help localize the firing fields of hippocampal place cells during formation and use of the hippocampal spatial representation ("cognitive map"). Of theoretical imp...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Casali G,Bush D,Jeffery K

更新日期：2019-03-05 00:00:00