Phosphorylation of actin Tyr-53 inhibits filament nucleation and elongation and destabilizes filaments.

abstract：:Amyotrophic lateral sclerosis is a neurodegenerative syndrome associated with 114 mutations in the gene encoding the cytosolic homodimeric enzyme Cu/Zn superoxide dismutase (SOD). In this article, we report that amyotrophic lateral sclerosis-associated SOD mutations with distinctly different disease progression can be...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lindberg MJ,Byström R,Boknäs N,Andersen PM,Oliveberg M

更新日期：2005-07-12 00:00:00

abstract：:The mammalian protein ZnT3 resides on synaptic vesicle membranes of zinc-containing neurons, suggesting its possible role in vesicular zinc transport. We show here that histochemically reactive zinc, corresponding to the zinc found within synaptic vesicles, was undetectable in the brains of mice with targeted disrupti...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Cole TB,Wenzel HJ,Kafer KE,Schwartzkroin PA,Palmiter RD

更新日期：1999-02-16 00:00:00

abstract：:sorLA (Sorting protein-related receptor) is a type-1 membrane protein of unknown function that is expressed in neurons. Its homology to sorting receptors that shuttle between the plasma membrane, endosomes, and the Golgi suggests a related function in neuronal trafficking processes. Because expression of sorLA is redu...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Andersen OM,Reiche J,Schmidt V,Gotthardt M,Spoelgen R,Behlke J,von Arnim CA,Breiderhoff T,Jansen P,Wu X,Bales KR,Cappai R,Masters CL,Gliemann J,Mufson EJ,Hyman BT,Paul SM,Nykjaer A,Willnow TE

更新日期：2005-09-20 00:00:00

abstract：:By adapting to sustained stimuli, hair cells in the internal ear retain their sensitivity to minute transient displacements. Because one model for adaptation asserts that this process is mediated by a myosin isozyme, we reasoned that we should be able to arrest adaptation by interfering with myosin's ATPase cycle thou...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gillespie PG,Hudspeth AJ

更新日期：1993-04-01 00:00:00

abstract：:The acid-sensing ion channel 1a (ASIC1a) is abundantly expressed in the amygdala complex and other brain regions associated with fear. Studies of mice with a disrupted ASIC1 gene suggested that ASIC1a may contribute to learned fear. To test this hypothesis, we generated mice overexpressing human ASIC1a by using the pa...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wemmie JA,Coryell MW,Askwith CC,Lamani E,Leonard AS,Sigmund CD,Welsh MJ

更新日期：2004-03-09 00:00:00

abstract：:Members of the ras gene family are activated as oncogenes in many different human cancers. To systematically determine the frequency at which such genes might be involved in the neoplastic process affecting a specific target tissue, urothelial cells, we surveyed a large series of urinary tract tumors for ras oncogenes...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Fujita J,Srivastava SK,Kraus MH,Rhim JS,Tronick SR,Aaronson SA

更新日期：1985-06-01 00:00:00

abstract：:As chips further shrink toward smaller scales, fabrication processes based on the self-assembly of individual particles into patterns or structures are often sought. One of the most popular techniques for two-dimensional assembly (self-assembled monolayers) is based on capillary forces acting on particles placed at a ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Aubry N,Singh P,Janjua M,Nudurupati S

更新日期：2008-03-11 00:00:00

abstract：:There is currently no consensus on the importance of climate change in Mesoamerican prehistory. Some invoke drought as a causal factor in major cultural transitions, including the abandonment of many sites at 900 CE, while others conclude that cultural factors were more important. This lack of agreement reflects the f...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bhattacharya T,Byrne R,Böhnel H,Wogau K,Kienel U,Ingram BL,Zimmerman S

更新日期：2015-02-10 00:00:00

abstract：:When long polymers such as DNA are in a highly concentrated state they may become entangled, leading to restricted self-diffusion. Here, we investigate the effect of molecular topology on diffusion in concentrated DNA solutions and find surprisingly large effects, even with molecules of modest length and concentration...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Robertson RM,Smith DE

更新日期：2007-03-20 00:00:00

abstract：:HOX11, a divergent homeodomain-containing transcription factor, was isolated from the breakpoint of the nonrandom t(10;14)(q24;q11) chromosome translocation found in human T cell acute lymphoblastic leukemias. The translocation places the HOX11 coding sequence under the transcriptional control of TCR alpha/delta regul...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hough MR,Reis MD,Singaraja R,Bryce DM,Kamel-Reid S,Dardick I,Breitman ML,Dubé ID

更新日期：1998-11-10 00:00:00

abstract：:The core protein of a small chondroitin/dermatan sulfate proteoglycan expressed by human fibroblasts and present in extracellular matrices in association with collagen has been cloned from a lambda gt11 fibroblast cDNA library. cDNA clones were isolated by use of antibodies specific for the intact proteoglycan and ant...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Krusius T,Ruoslahti E

更新日期：1986-10-01 00:00:00

abstract：:Myofibril formation was visualized in cultured live cardiomyocytes that were transfected with plasmids expressing green fluorescent protein (GFP) linked to the Z-band protein, alpha-actinin. The expression of this fluorescent protein provided an in vivo label for structures containing alpha-actinin. The GFP-alpha-acti...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Dabiri GA,Turnacioglu KK,Sanger JM,Sanger JW

更新日期：1997-08-19 00:00:00

abstract：:The intermediates and the rate-limiting step in the crystallization of deoxygenated sickle hemoglobin have been determined by a kinetic study with the use of electron microscopy. In slowly stirred solutions of deoxygenated hemoglobin S [Pumphrey, J. & Steinhardt, J. (1977) J. Mol. Biol. 112, 359--375], the sequential ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wilson SM,Makinen MW

更新日期：1980-02-01 00:00:00

abstract：:Methyl groups placed on varphiXsB1 replicative form DNA by the Escherichia coli B modification enzyme are located in the overlap between fragments Mbo II-3 and Alu I-2, a 61-base-pair DNA segment. Mutations that led to loss of susceptibility to restriction by E. coli B occurred within this segment at three positions s...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lautenberger JA,Kan NC,Lackey D,Linn S,Edgell MH,Hutchison CA 3rd

更新日期：1978-05-01 00:00:00

abstract：:The recent emergence and reemergence of viruses in the human population has highlighted the need to develop broader panels of therapeutic molecules. High-throughput screening assays opening access to untargeted steps of the viral replication cycle will provide powerful leverage to identify innovative antiviral molecul...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Fernandez J,Hassen-Khodja C,Georget V,Rose T,Jacob Y,Janin YL,Nisole S,Vidalain PO,Arhel NJ

更新日期：2021-01-12 00:00:00

abstract：:Infant acute lymphoblastic leukemia (ALL) with MLL gene rearrangements is characterized by early pre-B phenotype (CD10(-)/CD19(+)) and poor treatment outcome. The t(4;11), creating MLL-AF4 chimeric transcripts, is the predominant 11q23 chromosome translocation in infant ALL and is associated with extremely poor progno...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Taki T,Kano H,Taniwaki M,Sako M,Yanagisawa M,Hayashi Y

更新日期：1999-12-07 00:00:00

abstract：:The 82-residue amino-terminal sequences of simian virus 40 large tumor antigen (TAg) and small tumor antigen (tAg) are identical. Genetic analysis of TAg lacking amino acids 1-82 revealed that it was transformation-defective, as revealed by the agar growth assay, except when introduced in the presence of tAg. Since th...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Montano X,Millikan R,Milhaven JM,Newsom DA,Ludlow JW,Arthur AK,Fanning E,Bikel I,Livingston DM

更新日期：1990-10-01 00:00:00

abstract：:During exercise, increased energy demands are met by increased glucose production that occurs simultaneously with the increased glucose uptake. We had previously observed that, during exercise, metabolic clearance rate of glucose (MCR) increases markedly in normal, but only marginally in poorly controlled diabetic dog...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Miles PD,Yamatani K,Lickley HL,Vranic M

更新日期：1991-02-15 00:00:00

abstract：:In yeasts and mammals, PEX10 encodes an integral membrane protein with a C3HC4 RING finger motif in its C-terminal domain and is required for peroxisome biogenesis and matrix protein import. In humans, its dysfunction in peroxisome biogenesis leads to severe Zellweger Syndrome and infantile Refsum disease. Here we sho...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Schumann U,Wanner G,Veenhuis M,Schmid M,Gietl C

更新日期：2003-08-05 00:00:00

abstract：:4"'-Diazomalonyldigitoxin and its isomer, 3"'-diazomalonyldigitoxin, have been synthesized at high specific radioactivity and used as photolabels for the Na,K-ATPase (ATP phosphohydrolase, EC 3.6.1.3) purified from Electrophorus electricus. Photoaffinity labeling experiments using both type I and type II complexes of ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hall C,Ruoho A

更新日期：1980-08-01 00:00:00

abstract：:Methicillin-resistant Staphylococcus aureus (MRSA) is an emerging cause of catheter-associated urinary tract infection (CAUTI), which frequently progresses to more serious invasive infections. We adapted a mouse model of CAUTI to investigate how catheterization increases an individual's susceptibility to MRSA UTI. Thi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Walker JN,Flores-Mireles AL,Pinkner CL,Schreiber HL 4th,Joens MS,Park AM,Potretzke AM,Bauman TM,Pinkner JS,Fitzpatrick JAJ,Desai A,Caparon MG,Hultgren SJ

更新日期：2017-10-10 00:00:00

abstract：:The sequence of oligonucleotides produced by the action of bleomycin and ferrous ion on double- and single-stranded DNA has been determined. In the presence of ferrous ion, bleomycin promotes cleavage at G-T and G-C sequences, while high concentrations of ferrous ion alone result in strand scission that is not base sp...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Takeshita M,Grollman AP,Ohtsubo E,Ohtsubo H

更新日期：1978-12-01 00:00:00

abstract：:Identification of genomic regions that control tissue-specific gene expression is currently problematic. ChIP and high-throughput sequencing (ChIP-seq) of enhancer-associated proteins such as p300 identifies some but not all enhancers active in a tissue. Here we show that co-occupancy of a chromatin region by multiple...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： He A,Kong SW,Ma Q,Pu WT

更新日期：2011-04-05 00:00:00

abstract：:We solve a discrete version of the classic Plateau problem, which asks for a minimal surface spanning a given curve. Our algorithm is based on a network-flow formulation that finds minimal slabs, intuitively corresponding to minimal "surfaces" of prescribed thickness. We let the slab thickness approach zero in order t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hu TC,Kahng AB,Robins G

更新日期：1992-10-01 00:00:00

abstract：:The bright ultrafast pulses of X-ray Free-Electron Lasers allow investigation into the structure of matter under extreme conditions. We have used single pulses to ionize and probe water as it undergoes a phase transition from liquid to plasma. We report changes in the structure of liquid water on a femtosecond time sc...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Beyerlein KR,Jönsson HO,Alonso-Mori R,Aquila A,Bajt S,Barty A,Bean R,Koglin JE,Messerschmidt M,Ragazzon D,Sokaras D,Williams GJ,Hau-Riege S,Boutet S,Chapman HN,Tîmneanu N,Caleman C

更新日期：2018-05-29 00:00:00

abstract：:We have investigated whether the large (5- to 15-fold) stimulation of synthesis of growth hormone (somatotropin) observed upon exposure of a strain of rat pituitary (GH3) cells to dexamethasone (a synthetic glucocorticoid) arises from a stimulation of pregrowth hormone mRNA. A good agreement was observed between the s...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tushinski RJ,Sussman PM,Yu LY,Bancroft FC

更新日期：1977-06-01 00:00:00

abstract：:125I-Labeled L-fucose-albumin complex and rat preputial beta-glucuronidase are rapidly cleared from plasma after intravenous infusion. L-Fucose-albumin retards the plasma clearance of beta-glucuronidase whereas D-fucose-albumin is inactive. In vitro, 125I-labeled L-fucose-albumin is taken up into rat or rabbit alveola...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Shepherd VL,Lee YC,Schlesinger PH,Stahl PD

更新日期：1981-02-01 00:00:00

abstract：:Protein phosphorylation is a ubiquitous and one of the most effective means of regulating protein activity. Receptor phosphorylation is a key event in signal transduction. The question, therefore, that arises is whether this modulatory mechanism might produce functional changes in a membrane receptor in the absence of...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ferrer-Montiel AV,Montal MS,Díaz-Muñoz M,Montal M

更新日期：1991-11-15 00:00:00

abstract：:Let X = Omega/Gamma be a compact quotient of an irreducible bounded symmetric domain Omega of rank >/=2 by a discrete group omega of automorphisms without fixed points. It is well known that the Kähler-Einstein metric g on X carries seminegative curvature (in the sense of Griffiths). I show that any Hermitian metric h...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mok N

更新日期：1986-04-01 00:00:00

abstract：:We report the synthesis of a probe that permits the visualization by electron microscopy of acidic organelles in intact cells. This probe, 3-(2,4-dinitroanilino)-3'-amino-N-methyldipropylamine (DAMP), is a basic congener of dinitrophenol that readily diffuses into intact cells. Its primary and tertiary amino groups (a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Anderson RG,Falck JR,Goldstein JL,Brown MS

更新日期：1984-08-01 00:00:00