当前位置： SCI文献检索 > PLACENTA期刊下所有文献 > Differential apoptosis in BeWo cells after infection with highly (RH) or moderately (ME49) virulent strains of Toxoplasma gondii is related to the cytokine profile secreted, the death receptor Fas expression and phosphorylated ERK1/2 expression.

Differential apoptosis in BeWo cells after infection with highly (RH) or moderately (ME49) virulent strains of Toxoplasma gondii is related to the cytokine profile secreted, the death receptor Fas expression and phosphorylated ERK1/2 expression.

Abstract：

INTRODUCTION:Alterations of apoptosis are commonly associated with pregnancy complications and abortion. Modulation of apoptosis is a relevant feature of Toxoplasma gondii infection and it is related to parasite strain types. The aim of the present study was to evaluate the possible factors that are involved in the differential apoptosis of BeWo cells infected with distinct T. gondii strain types. METHODS:Human trophoblastic cells (BeWo cell line) were infected with RH or ME49 strains, the cytokine production was measured and the phosphorylation of anti-apoptotic ERK1/2 protein was analyzed. Also, cells were treated with different cytokines, infected with RH or ME49 strain, and analyzed for apoptosis index and Fas/CD95 death receptor expression. RESULTS:ME49-infected BeWo cells exhibited a predominantly pro-inflammatory cytokine profile, whereas cells infected with RH strain had a higher production of anti-inflammatory cytokines. Also, the incidence of apoptosis was higher in ME49-infected cells, which have been treated with pro-inflammatory cytokines compared to cells infected with RH and treated with anti-inflammatory cytokines. Moreover, Fas/CD95 expression was higher in cells infected with either ME49 or RH strain and treated with pro-inflammatory cytokines compared to anti-inflammatory cytokine treatment. The phosphorylation of ERK1/2 protein increased after 24 h of infection only with the RH strain. CONCLUSION:These results suggest that opposing mechanisms of interference in apoptosis of BeWo cells after infection with RH or ME49 strains of T. gondii can be associated with the differential cytokine profile secreted, the Fas/CD95 expression and the phosphorylated ERK1/2 expression.

journal_name

Placenta

journal_title

Placenta

authors

Angeloni MB,Guirelli PM,Franco PS,Barbosa BF,Gomes AO,Castro AS,Silva NM,Martins-Filho OA,Mineo TW,Silva DA,Mineo JR,Ferro EA

doi

10.1016/j.placenta.2013.09.005

subject

Has Abstract

pub_date

2013-11-01 00:00:00

pages

973-82

issue

11

eissn

0143-4004

issn

1532-3102

pii

S0143-4004(13)00731-5

journal_volume

34

pub_type

杂志文章

相关文献

PLACENTA文献大全
  • Invasion of trophoblast cell lines is inhibited by miR-93 via MMP-2.

    abstract：:Preeclampsia (PE) is a serious pregnancy-related syndrome, which is characterized by gestational hypertension and proteinuria. The microRNA-93 (miR-93) is upregulated in the maternal plasma of patients with PE. However, the functional role of miR-93 in PE remains unknown. Here, we identified that miR-93 inhibits troph...

    journal_title：Placenta

    pub_type： 杂志文章,收录出版

    doi：10.1016/j.placenta.2017.03.008

    authors： Pan Q,Niu H,Cheng L,Li X,Zhang Q,Ning Y

    更新日期：2017-05-01 00:00:00

  • Uptake of 1-methyl-4-phenylpyridinium (MPP+) by the JAR human placental choriocarcinoma cell line: comparison with 5-hydroxytryptamine.

    abstract：:The aim of this work was to characterize the uptake of 1-methyl-4-phenylpyridinium (MPP(+)) in the JAR human choriocarcinoma cell line. As JAR cells, as well as the placenta, express the neuronal serotonin transporter (SERT), a comparison between the uptake of (3)H-MPP(+) and (3)H-serotonin ((3)H-5HT) was made. Specif...

    journal_title：Placenta

    pub_type： 杂志文章

    doi：10.1053/plac.2002.0917

    authors： Martel F,Keating E

    更新日期：2003-04-01 00:00:00

  • A novel hysteroscopic technique for the accurate biopsy of decidua parietalis and basalis.

    abstract：BACKGROUND:Discrepancies in the results from studies of early events in human trophoblast invasion of decidua have been acknowledged and are attributed largely to deficiencies in the accuracy of sampling of the decidual tissue used in the research. We describe a novel technique of biopsy of decidua parietalis and basal...

    journal_title：Placenta

    pub_type： 杂志文章

    doi：10.1016/j.placenta.2012.02.018

    authors： Talaulikar VS,Bax BE,Page NM,Manyonda I

    更新日期：2012-06-01 00:00:00

  • Downregulation of protein kinase C by phorbol ester increases expression of epidermal growth factor receptors in transformed trophoblasts and amplifies human chorionic gonadotropin production.

    abstract：:Epidermal growth factor (EGF) and its homologue, transforming growth factor-alpha (TGF-alpha), regulate human chorionic gonadotropin (hCG) synthesis in the human placenta. The current study was designed to investigate the involvement of the protein kinase C pathway in EGF-mediated hCG-beta production by JAr choriocarc...

    journal_title：Placenta

    pub_type： 杂志文章

    doi：10.1016/s0143-4004(98)91040-2

    authors： Baker VL,Murai JT,Taylor RN

    更新日期：1998-09-01 00:00:00

  • Studies on the localization of 5-hydroxytryptamine and its receptors in human placenta.

    abstract：:The localization of 5-hydroxytryptamine (5-HT) and its receptors in human placenta was studied under light and electron microscopy using immunohistochemistry and in situ hybridization. The syncytiotrophoblast, cytotrophoblast, stromal cells and decidual cells in human placentae all appeared to be 5-HT immunoreactive. ...

    journal_title：Placenta

    pub_type： 杂志文章

    doi：10.1016/s0143-4004(98)90027-3

    authors： Huang WQ,Zhang CL,Di XY,Zhang RQ

    更新日期：1998-11-01 00:00:00

  • Corticotrophin-releasing factor and urocortin inhibit system A activity in term human placental villous explants.

    abstract：:Plasma corticotrophin-releasing factor (CRF) and urocortin are elevated in preterm labour and/or fetal growth restriction (FGR). FGR is associated with reduced placental system A amino acid transporter activity and in vitro data suggest altered endocrine status could be responsible. Here we test the hypothesis that CR...

    journal_title：Placenta

    pub_type： 杂志文章

    doi：10.1016/j.placenta.2010.10.015

    authors： Giovannelli A,Greenwood SL,Desforges M,Sibley CP,Petraglia F

    更新日期：2011-01-01 00:00:00

  • Expression and localisation of FoxO3 and FoxO4 in human placenta and fetal membranes.

    abstract：:Forkhead box O (FoxO) proteins regulate inflammation, extracellular matrix (ECM) remodelling and apoptosis. We have previously identified FoxO1 proteins in human gestational tissues, and demonstrated a link between FoxO1 and rupture of fetal membranes. There is, however, no data available on the expression and localis...

    journal_title：Placenta

    pub_type： 杂志文章

    doi：10.1016/j.placenta.2010.09.009

    authors： Lappas M,Lim R,Riley C,Menon R,Permezel M

    更新日期：2010-12-01 00:00:00

  • Transplacental lactate dehydrogenase-elevating virus (LDV) transmission: immune inhibition of umbilical cord infection, and correlation of fetal virus susceptibility with development of F4/80 antigen expression.

    abstract：:Maternal-to-fetal transmission of the murine lactate dehydrogenase-elevating virus (LDV) has been previously shown to be regulated by maternal immunity as well as gestational age. For the present study, the role of maternal immunity in placental and umbilical cord virus protection was studied, and virus targeting of u...

    journal_title：Placenta

    pub_type： 杂志文章

    doi：10.1053/plac.2002.0829

    authors： Zitterkopf NL,Haven TR,Huela M,Bradley DS,Cafruny WA

    更新日期：2002-05-01 00:00:00

  • SNAT expression in rat placenta.

    abstract：:Amino acid transport System A (SysA) activity is present within the rodent and human placentas. Inhibition of this transport system is associated with fetal growth retardation. Several cDNAs encoding SysA transport proteins have been discovered, and their presence documented within the human placenta. We have demonstr...

    journal_title：Placenta

    pub_type： 杂志文章

    doi：10.1016/j.placenta.2005.05.002

    authors： Novak D,Lehman M,Bernstein H,Beveridge M,Cramer S

    更新日期：2006-04-01 00:00:00

  • Altered maternal and placental lipid metabolism and fetal fat development in obesity: Current knowledge and advances in non-invasive assessment.

    abstract：:Abnormal maternal lipid profiles, a hallmark of increased maternal adiposity, are associated with pregnancy complications such as preeclampsia and gestational diabetes, and offspring long-term metabolic health is impacted as the consequence of altered fetal growth, physiology and often iatrogenic prematurity. The meta...

    journal_title：Placenta

    pub_type： 杂志文章,评审

    doi：10.1016/j.placenta.2018.05.011

    authors： Delhaes F,Giza SA,Koreman T,Eastabrook G,McKenzie CA,Bedell S,Regnault TRH,de Vrijer B

    更新日期：2018-09-01 00:00:00

  • Role of low placental share in twin-twin transfusion syndrome complicated by intrauterine growth restriction.

    abstract：OBJECTIVES:Prior studies have demonstrated that donor twin survival following treatment of twin-twin transfusion syndrome (TTTS) was highly associated with donor intrauterine growth restriction (IUGR). Here, we hypothesized that donor IUGR may be attributed in part to low placental share. STUDY DESIGN:The study popula...

    journal_title：Placenta

    pub_type： 杂志文章

    doi：10.1016/j.placenta.2011.05.009

    authors： Grubbs BH,Benirschke K,Korst LM,Llanes A,Yedigarova L,Chmait RH

    更新日期：2011-08-01 00:00:00

  • Human placental multipotent mesenchymal stromal cells modulate trophoblast migration via Rap1 activation.

    abstract：INTRODUCTION:Little is known about the interaction between human placental multipotent mesenchymal stromal cell (hPMSC) and trophoblast. We hypothesize that hPMSCs produce hepatocyte growth factor (HGF) which may interact with trophoblasts and regulate their migration during placentation. METHODS:hPMSCs were isolated ...

    journal_title：Placenta

    pub_type： 杂志文章

    doi：10.1016/j.placenta.2013.06.311

    authors： Chen CP,Huang JP,Chu TY,Aplin JD,Chen CY,Wu YH

    更新日期：2013-10-01 00:00:00

  • Villous trophoblast apoptosis is elevated and restricted to cytotrophoblasts in pregnancies complicated by preeclampsia, IUGR, or preeclampsia with IUGR.

    abstract：:Human placental villi are surfaced by an outer multinucleated syncytiotrophoblast and underlying mononucleated cytotrophoblasts. Conflicting data have attributed one, or the other, of these villous trophoblast phenotypes to undergo enhanced apoptosis in complicated pregnancies, compared to term, normotensive pregnanci...

    journal_title：Placenta

    pub_type： 杂志文章

    doi：10.1016/j.placenta.2012.01.017

    authors： Longtine MS,Chen B,Odibo AO,Zhong Y,Nelson DM

    更新日期：2012-05-01 00:00:00

  • Ly6e expression is restricted to syncytiotrophoblast cells of the mouse placenta.

    abstract：:In the present study, we characterized the expression of lymphocyte antigen 6, locus E (Ly6e) in mouse placental trophoblast. We identified Ly6e mRNA expression in trophoblast stem (TS) cells by a gene expression screen. In vivo, Ly6e was first detectable by mRNA in situ hybridization in the chorion beginning at E8.5 ...

    journal_title：Placenta

    pub_type： 杂志文章

    doi：10.1016/j.placenta.2013.05.011

    authors： Hughes M,Natale BV,Simmons DG,Natale DR

    更新日期：2013-09-01 00:00:00

  • Lipid deposition and metabolism in rat placenta during gestation.

    abstract：:Changes in placental lipid content in rats at the 15th and 20th days of pregnancy were investigates. It was found that progress of gestation is accompanied by a significant increase in triglycerides per mg placental DNA. Maternal starvation caused additional increase in the placental triglyceride content. By incubatio...

    journal_title：Placenta

    pub_type： 杂志文章

    doi：10.1016/s0143-4004(80)80033-6

    authors： Diamant YZ,Diamant S,Freinkel N

    更新日期：1980-10-01 00:00:00

  • A mathematical model of intervillous blood flow in the human placentone.

    abstract：:We present a mathematical model for maternal blood flow in a placental circulatory unit (a placentone), describing flow of maternal blood via Darcy's law and steady advective transport of a dissolved nutrient. The method of images and computational integration along streamlines are employed to find flow and solute con...

    journal_title：Placenta

    pub_type： 杂志文章

    doi：10.1016/j.placenta.2009.11.003

    authors： Chernyavsky IL,Jensen OE,Leach L

    更新日期：2010-01-01 00:00:00

  • Macroscopic and microscopic aspects of collared peccary and white-lipped peccary placenta.

    abstract：:This study examines middle and late gestational placentae from 13 Tayassu tajacu (collared peccary) and 3 Tayassu pecari (white-lipped peccary), which are Artiodactyla belonging to the Family Tayassuidae. The chorionic sac of Tayassu species is diffuse and chorioallantoic. These epitheliochorial placentae show no trop...

    journal_title：Placenta

    pub_type： 杂志文章

    doi：10.1016/j.placenta.2005.02.017

    authors： Santos TC,Dantzer V,Jones CJ,Oliveira MF,Miglino MA

    更新日期：2006-02-01 00:00:00

  • Placental function and structure at term is altered in broodmares fed with cereals from mid-gestation.

    abstract：INTRODUCTION:Feeding pregnant broodmares with cereal concentrates has been shown to increase maternal insulin resistance and affect foal metabolism in the short and long-term. These effects are likely to be mediated by the placenta. Here, we investigated feto-placental biometry and placental structure and function at t...

    journal_title：Placenta

    pub_type： 杂志文章

    doi：10.1016/j.placenta.2018.02.003

    authors： Robles M,Peugnet P,Dubois C,Piumi F,Jouneau L,Bouchez O,Aubrière MC,Dahirel M,Aioun J,Wimel L,Couturier-Tarrade A,Chavatte-Palmer P

    更新日期：2018-04-01 00:00:00

  • Review: Differential placental macrovascular and microvascular endothelial dysfunction in gestational diabetes.

    abstract：:Human endothelial dysfunction is a common feature in many diseases of pregnancy, such as gestational diabetes (GD). Metabolic changes include abnormal synthesis of nitric oxide (NO) and abnormal membrane transport of l-arginine and adenosine in primary cultures of human umbilical vein (HUVEC, macrovascular) and placen...

    journal_title：Placenta

    pub_type： 杂志文章,评审

    doi：10.1016/j.placenta.2010.12.011

    authors： Sobrevia L,Abarzúa F,Nien JK,Salomón C,Westermeier F,Puebla C,Cifuentes F,Guzmán-Gutiérrez E,Leiva A,Casanello P

    更新日期：2011-03-01 00:00:00

  • Endothelium-derived relaxing factor and cyclic GMP-dependent vasorelaxation in human chorionic plate arteries.

    abstract：:Endothelium derived relaxing factor (EDRF), now widely believed to be nitric oxide (NO), may play an important part in the control of fetoplacental vascular tone. To further explore this role we have determined the relaxation responses to exogenous NO and examined the temporal relationship between intracellular concen...

    journal_title：Placenta

    pub_type： 杂志文章

    doi：10.1016/0143-4004(94)90004-3

    authors： Hull AD,White CR,Pearce WJ

    更新日期：1994-06-01 00:00:00

  • Discordance of umbilical coiling index between recipients and donors in twin-twin transfusion syndrome.

    abstract：INTRODUCTION:To compare the intertwin umbilical cord coiling in twin-twin transfusion syndrome (TTTS) before fetoscopic laser treatment and to correlate these with Doppler findings in both twins. METHODS:We performed a prospective study using three-dimensional (3D) ultrasound with color Doppler imaging of the umbilica...

    journal_title：Placenta

    pub_type： 杂志文章

    doi：10.1016/j.placenta.2019.01.013

    authors： Bamberg C,Diemert A,Glosemeyer P,Tavares de Sousa M,Hecher K

    更新日期：2019-01-15 00:00:00

  • 2005 Trophoblast Research Award Lecture: Defects in the keratin cytoskeleton disrupt normal murine placental development and trophoblast cell function.

    abstract：:The keratin cytoskeleton is present in all trophoblast cell subtypes of the mouse and human placenta and is required to maintain the structural integrity of these cells. Recently, various genetic mouse models have shown that a normal keratin network is necessary for placental development. Keratin-deficiency leads to t...

    journal_title：Placenta

    pub_type：

    doi：10.1016/j.placenta.2007.01.006

    authors： Watson ED

    更新日期：2007-04-01 00:00:00

  • Corticosteroid metabolism in vitro by human placenta, fetal membranes and decidua in early and late gestation.

    abstract：:Corticosteroid conversion by early (7 to 10 weeks) and late (36 to 42 weeks) human placenta, fetal membranes and decidua was studied. Tritiated cortisol, cortisone, corticosterone, 11-dehydrocorticosterone (DHC) and 11-deoxycorticosterone (DOC) were incubated in vitro with homogenates of these tissues and extracts chr...

    journal_title：Placenta

    pub_type： 杂志文章

    doi：10.1016/s0143-4004(81)80025-2

    authors： López Bernal A,Craft IL

    更新日期：1981-10-01 00:00:00

  • The effect of labour and placental separation on the shedding of syncytiotrophoblast microparticles, cell-free DNA and mRNA in normal pregnancy and pre-eclampsia.

    abstract：:The clinical features of the maternal syndrome of pre-eclampsia can be explained by generalised maternal endothelial cell dysfunction, which is a part of a more global maternal systemic inflammatory response. There is growing evidence that these effects are associated with the shedding of cellular debris, including sy...

    journal_title：Placenta

    pub_type： 杂志文章

    doi：10.1016/j.placenta.2008.08.018

    authors： Reddy A,Zhong XY,Rusterholz C,Hahn S,Holzgreve W,Redman CW,Sargent IL

    更新日期：2008-11-01 00:00:00

  • Free amino acid concentrations in human first and third trimester placental villi.

    abstract：:Homogenates of first (10.1 +/- 1.0 weeks) and third trimester placental villi were analysed for free amino acid concentrations. As has been previously reported, several amino acids showed increased concentrations during early pregnancy when compared to term. In addition, marked differences were seen in the levels of e...

    journal_title：Placenta

    pub_type： 杂志文章

    doi：10.1016/0143-4004(94)90037-x

    authors： Sooranna SR,Burston D,Ramsay B,Steer PJ

    更新日期：1994-10-01 00:00:00

  • What can comparative studies of placental structure tell us?--A review.

    abstract：:The diversity of placental structures in Eutherian mammals is such that drawing generalizations from the definitive forms is problematic. There are always areas of reduced interhaemal distance whether the placenta is epitheliochorial, synepitheliochorial, endotheliochorial or haemochorial. However, the thinning may be...

    journal_title：Placenta

    pub_type： 杂志文章,评审

    doi：10.1016/j.placenta.2004.01.011

    authors： Enders AC,Carter AM

    更新日期：2004-04-01 00:00:00

  • Chronic reduction in fetal blood flow is associated with placental infarction.

    abstract：:The placenta receives two arterial blood supplies, i.e. one maternal and one fetal. It has been suggested that placental infarction should occur only if both blood supplies are compromised (Wigglesworth, 1984). This hypothesis has not been tested. Haemosiderosis of the trophoblast basement membrane (TBMH) has recently...

    journal_title：Placenta

    pub_type： 临床试验,杂志文章,随机对照试验

    doi：10.1016/0143-4004(95)90005-5

    authors： McDermott M,Gillan JE

    更新日期：1995-03-01 00:00:00

  • Investigations into the molecular heterogeneity of pregnancy-specific beta 1-glycoprotein (SP1).

    abstract：:The molecular heterogeneity of pregnancy-specific beta 1-glycoprotein (SP1) was examined by analytical immunoelectrophoresis and a radioimmunostaining technique of immunoelectrophoretic plates. Analytical crossed immunoelectrophoretic analysis and radioimmunostaining of these plates demonstrated the presence of normal...

    journal_title：Placenta

    pub_type： 杂志文章

    doi：10.1016/s0143-4004(81)80006-9

    authors： Hindersson P,Teisner B,Folkersen J,Grudzinskas JG,Westergaard JG

    更新日期：1981-07-01 00:00:00

  • Relaxant effects of hexosamines on isolated small human placental arteries.

    abstract：:Wharton's jelly contains large amounts of hyaluronic acid, and glucosamine is an important constituent of this macromolecule. In order to evaluate the placental vascular effects of this aminosugar, small chorionic and stem villous arteries were dissected from placental specimens obtained at normal term vaginal deliver...

    journal_title：Placenta

    pub_type： 杂志文章

    doi：10.1016/0143-4004(87)90069-5

    authors： Uldbjerg N,Allen J,Maigaard S,Forman A

    更新日期：1987-07-01 00:00:00

  • Nitrotyrosine immunostaining correlates with increased extracellular matrix: evidence of postplacental hypoxia.

    abstract：:Nitrotyrosine residues (NT), an index of oxidative stress arising from peroxynitrite formation and action, are found in placental vasculature of pregnancies complicated by pre-eclampsia (PE) or pregestational insulin-dependent diabetes mellitus (IDDM). This study correlates conventional placental pathology with NT imm...

    journal_title：Placenta

    pub_type： 杂志文章

    doi：10.1053/plac.2001.0634

    authors： Stanek J,Eis AL,Myatt L

    更新日期：2001-04-01 00:00:00