Uptake of 1-methyl-4-phenylpyridinium (MPP+) by the JAR human placental choriocarcinoma cell line: comparison with 5-hydroxytryptamine.

Abstract:

:The aim of this work was to characterize the uptake of 1-methyl-4-phenylpyridinium (MPP(+)) in the JAR human choriocarcinoma cell line. As JAR cells, as well as the placenta, express the neuronal serotonin transporter (SERT), a comparison between the uptake of (3)H-MPP(+) and (3)H-serotonin ((3)H-5HT) was made. Specific uptake of (3)H-MPP(+) (0.2 microM ) was temperature-, Na(+)- and potential-dependent. 5HT and MPP(+) reduced (3)H-MPP(+) specific uptake (for 5HT, its IC(50) was found to be 4 microM ). The SERT inhibitors desipramine and fluoxetine also inhibited (3)H-MPP(+) specific uptake (with IC(50)s of 189 and 0.92 microM, respectively). The inhibitors of the extraneuronal monoamine transporter (EMT) and of the organic cation transporter type 2 (OCT2), corticosterone and decynium22, had no effect on (3)H-MPP(+) specific uptake, but cyanine863 concentration-dependently reduced it (with an IC(50) of 23 microM ). Specific uptake of (3)H-5HT (0.2 microM ) by JAR cells was temperature-, Na(+)- and potential-dependent. 5HT, MPP(+), desipramine and fluoxetine concentration-dependently inhibited (3)H-5HT specific uptake (with IC(50)s of 1.9 microM, 50 microM, 0.17 microM and 0.046 microM, respectively). Corticosterone showed no effect, but decynium22 and cyanine863 significantly reduced(3) H-5HT specific uptake. For cyanine863, its IC(50) was found to be 11 microM. In conclusion, the results suggest that: (1) uptake of (3)H-5HT by JAR cells occurs exclusively through SERT; (2) uptake of(3) H-MPP(+) by JAR cells involves SERT and also another transporter; (3) neither EMT nor OCT2 are functionally present in JAR cells.

journal_name

Placenta

journal_title

Placenta

authors

Martel F,Keating E

doi

10.1053/plac.2002.0917

keywords:

subject

Has Abstract

pub_date

2003-04-01 00:00:00

pages

361-9

issue

4

eissn

0143-4004

issn

1532-3102

pii

S0143400402909173

journal_volume

24

pub_type

杂志文章

相关文献

PLACENTA文献大全
  • The effect of labour and placental separation on the shedding of syncytiotrophoblast microparticles, cell-free DNA and mRNA in normal pregnancy and pre-eclampsia.

    abstract::The clinical features of the maternal syndrome of pre-eclampsia can be explained by generalised maternal endothelial cell dysfunction, which is a part of a more global maternal systemic inflammatory response. There is growing evidence that these effects are associated with the shedding of cellular debris, including sy...

    journal_title:Placenta

    pub_type: 杂志文章

    doi:10.1016/j.placenta.2008.08.018

    authors: Reddy A,Zhong XY,Rusterholz C,Hahn S,Holzgreve W,Redman CW,Sargent IL

    更新日期:2008-11-01 00:00:00

  • A limitation of the method for siRNA delivery into primary human cytotrophoblast cells.

    abstract::Transfection using cationic lipid reagents such as DharmaFECT2 is an efficient tool for introducing siRNA into primary human cytotrophoblast cells. However, we now report a limitation to the use of this method as the concentration of DharamFECT2 needed for effective siRNA delivery causes ligand-independent activation ...

    journal_title:Placenta

    pub_type: 杂志文章

    doi:10.1016/j.placenta.2010.11.013

    authors: Desforges M,Westwood M

    更新日期:2011-02-01 00:00:00

  • Oestrogen and progesterone receptors in human term placenta. Measurement by binding assays and immunological methods.

    abstract::Oestrogen and progesterone receptors have been implicated in human placental steroidogenesis, but studies developed to measure their concentration in this tissue at term yielded discrepant results. Potential inaccuracy of the binding assays, the technique used in previous studies, might influence the discrepancy. In t...

    journal_title:Placenta

    pub_type: 杂志文章

    doi:10.1016/0143-4004(89)90049-0

    authors: Rivera J,Cano A

    更新日期:1989-11-01 00:00:00

  • Angiotensin II and its different receptor subtypes in placenta and fetal membranes.

    abstract::The recent discovery of a local renin-angiotensin system in trophoblastic tissues has raised many questions regarding its role in the physiology of normal gestation and its implications in the pathophysiology of hypertension during pregnancy. In this article, the authors first review the most interesting aspects of th...

    journal_title:Placenta

    pub_type: 杂志文章,评审

    doi:10.1016/s0143-4004(96)80003-8

    authors: Kalenga MK,de Gasparo M,Thomas K,de Hertogh R

    更新日期:1996-03-01 00:00:00

  • DNA content and ploidy level of bovine placentomal trophoblast giant cells.

    abstract::Cytophotometric measurement of the DNA content of Feulgen-stained nuclei in touch preparations of bovine placentomes (n =5) revealed that 8C nuclei occurred in all, 16C nuclei in two, and 32C nuclei in one specimen. The determination of ploidy level by in situ hybridization with a Y-chromosome specific DNA probe showe...

    journal_title:Placenta

    pub_type: 杂志文章

    doi:10.1053/plac.1999.0402

    authors: Klisch K,Hecht W,Pfarrer C,Schuler G,Hoffmann B,Leiser R

    更新日期:1999-07-01 00:00:00

  • Multiple human papillomavirus types replicate in 3A trophoblasts.

    abstract::Human papillomavirus (HPV) are more prevalent in spontaneous abortions than elect abortions and preferentially infect the trophoblasts. Related to this, HPV type 16 has been shown to productively replicate in 3A trophoblasts in tissue culture. Extending these earlier studies, the described study addresses the issue wh...

    journal_title:Placenta

    pub_type: 杂志文章

    doi:10.1016/j.placenta.2007.08.005

    authors: You H,Liu Y,Agrawal N,Prasad CK,Edwards JL,Osborne AF,Korourian S,Lowery CL,Hermonat PL

    更新日期:2008-01-01 00:00:00

  • The human placenta is encircled by a ring of smooth muscle cells.

    abstract::The marginal zone of the human term placenta was studied by transmission electron microscopy and immunohistochemistry using antibodies against cytoskeletal filaments, extracellular matrix molecules and endothelial markers. The marginal sinus of the intervillous space is separated from the chorionic and basal plates by...

    journal_title:Placenta

    pub_type: 杂志文章

    doi:10.1053/plac.1999.0455

    authors: Nanaev AK,Kosanke G,Kemp B,Frank HG,Huppertz B,Kaufmann P

    更新日期:2000-01-01 00:00:00

  • Patterns of expression of Bax, Bcl-2 and Bcl-x(L) in the implantation site in rat during pregnancy.

    abstract::The uterine endometrium responds to blastocyst implantation with extensive proliferation and differentiation of stromal cells into decidual cells, forming the antimesometrial and mesometrial decidua. These undergo regression by apoptosis but as this process occurs at different time periods suggest that there is spatia...

    journal_title:Placenta

    pub_type: 杂志文章

    doi:10.1016/j.placenta.2004.09.008

    authors: Correia-da-Silva G,Bell SC,Pringle JH,Teixeira NA

    更新日期:2005-11-01 00:00:00

  • Progesterone stimulates the expression of aminopeptidase A/angiotensinase in human choriocarcinoma cells.

    abstract::In human placenta aminopeptidase A (APA), a principal enzyme that converts angiotensin II to angiotensin III, seems to be involved in angiotensin II metabolism during pregnancy. In this study, we investigated the possible effects of progesterone and estrogen on APA mRNA and protein levels in choriocarcinoma cells as a...

    journal_title:Placenta

    pub_type: 杂志文章

    doi:10.1053/plac.2001.0741

    authors: Katsumata Y,Nomura S,Ino K,Iwanaga K,Kurosawa N,Ito T,Okada M,Tsujimoto M,Kikkawa F,Mizutani S

    更新日期:2001-11-01 00:00:00

  • Human placental mitochondrial respiration and its regulation by adenine nucleotides.

    abstract::Respiratory parameters were studied in mitochondria from human placenta. Respiratory control and ADP/O ratios were low in this preparation. The adenine nucleotide content of placental mitochondria was found to be only one quarter of that found for adult uterine muscle tissue mitochondria prepared in the same way. Load...

    journal_title:Placenta

    pub_type: 杂志文章

    doi:10.1016/s0143-4004(85)80049-7

    authors: Illsley NP,Coade SB,Harkness RA

    更新日期:1985-05-01 00:00:00

  • Expression of pregnancy-associated plasma protein-A (PAPP-A) during human villous trophoblast differentiation in vitro.

    abstract::Pregnancy-associated placental protein-A (PAPP-A), first isolated from maternal serum, has been identified as a metalloprotease cleaving insulin-like growth factor binding protein-4 (IGFBP-4). The source of PAPP-A during pregnancy is unclear. We therefore investigated PAPP-A expression during in vitro human villous cy...

    journal_title:Placenta

    pub_type: 杂志文章

    doi:10.1053/plac.2002.0944

    authors: Guibourdenche J,Frendo JL,Pidoux G,Bertin G,Luton D,Muller F,Porquet D,Evain-Brion D

    更新日期:2003-05-01 00:00:00

  • Changes in endometrial ezrin and cytokeratin 18 expression during bovine implantation and in caruncular endometrial spheroids in vitro.

    abstract:INTRODUCTION:The feto-maternal interface during bovine implantation was studied in vivo and using three-dimensional bovine endometrial (BCECph) and trophoblast spheroids (CCS), each with underlying fibroblasts. METHODS:The expression of ezrin and cytokeratin 18 (CK18) was analyzed via immunohistochemistry (IHC), RT-PC...

    journal_title:Placenta

    pub_type: 杂志文章

    doi:10.1016/j.placenta.2015.06.001

    authors: Haeger JD,Hambruch N,Dantzer V,Hoelker M,Schellander K,Klisch K,Pfarrer C

    更新日期:2015-08-01 00:00:00

  • Nitric oxide (NO) reversed TNF-α inhibition of trophoblast interaction with endothelial cellular networks.

    abstract:INTRODUCTION:The interaction between trophoblast cells and maternal uterine endothelium is important for placental vascular modelling. Nitric oxide (NO) is a potent vasorelaxant that regulates systemic blood pressure and is reduced in preeclampsia. NO may affect placental cell interaction. OBJECTIVES:This study was to...

    journal_title:Placenta

    pub_type: 杂志文章

    doi:10.1016/j.placenta.2014.03.009

    authors: Xu B,Charlton F,Makris A,Hennessy A

    更新日期:2014-06-01 00:00:00

  • Placental P-glycoprotein and breast cancer resistance protein: influence of polymorphisms on fetal drug exposure and physiology.

    abstract::Recent studies have illustrated the importance of placental drug transport proteins, such as P-glycoprotein (Pgp) and breast cancer resistance protein (BCRP) in limiting fetal exposure to drugs and toxins. Moreover, increasing evidence supports a role for Pgp and BCRP in the normal development and physiological functi...

    journal_title:Placenta

    pub_type: 杂志文章,评审

    doi:10.1016/j.placenta.2010.02.010

    authors: Hutson JR,Koren G,Matthews SG

    更新日期:2010-05-01 00:00:00

  • Placental histological lesions in fetal and neonatal alloimmune thrombocytopenia: A retrospective cohort study of 21 cases.

    abstract:BACKGROUND:Alloimmunization against human platelet antigens (HPAs) can occur prenatally and induce fetal/neonatal alloimmune thrombocytopenia (FNAIT). The aim of this study was to identify placental histological features associated with platelet alloimmunization and their clinical significance. METHODS:This study exam...

    journal_title:Placenta

    pub_type: 杂志文章

    doi:10.1016/j.placenta.2016.10.009

    authors: Dubruc E,Lebreton F,Giannoli C,Rabilloud M,Huissoud C,Devouassoux-Shisheboran M,Allias F

    更新日期:2016-12-01 00:00:00

  • Localization of three subtypes of Fc gamma receptors in human placenta by immunohistochemical analysis.

    abstract::We investigated the localization of the three subtypes of (Fc gamma R) in the normal human placenta using immunohistochemical and immunocytochemical techniques with specific monoclonal antibodies. The analysis revealed that Fc gamma RI was expressed on Hofbauer cells, that Fc gamma RII was expressed on Hofbauer cells ...

    journal_title:Placenta

    pub_type: 杂志文章

    doi:10.1016/0143-4004(91)90506-b

    authors: Kameda T,Koyama M,Matsuzaki N,Taniguchi T,Saji F,Tanizawa O

    更新日期:1991-01-01 00:00:00

  • Pregnancies complicated by retained placenta: sex ratio and relation to pre-eclampsia.

    abstract::Pre-eclampsia and placenta accreta have opposite histological features of placentation. This study set out to test the hypotheses that the sex ratios in these two pregnancy complications are opposite and that these conditions are mutually exclusive. A population-based database covering all deliveries in South Australi...

    journal_title:Placenta

    pub_type: 杂志文章

    doi:10.1016/s0143-4004(98)90017-0

    authors: Khong TY,Staples A,Chan AS,Keane RJ,Wilkinson CS

    更新日期:1998-11-01 00:00:00

  • Lysophosphatidic acid increases the production of pivotal mediators of decidualization and vascularization in the rat uterus.

    abstract:INTRODUCTION:The decidual reaction and the formation of new vessels in the uterus are two crucial processes during embryo implantation. Previously, we observed that lysophosphatidic acid (LPA) increases cyclooxygenase-2 derived - prostaglandin E2 production during implantation in the rat uterus and that it augments the...

    journal_title:Placenta

    pub_type: 杂志文章

    doi:10.1016/j.placenta.2013.06.001

    authors: Beltrame JS,Sordelli MS,Cella M,Perez Martinez S,Franchi AM,Ribeiro ML

    更新日期:2013-09-01 00:00:00

  • Characterization of receptors for murine pregnancy specific glycoproteins 17 and 23.

    abstract::In primates and rodents, trophoblast cells synthesize and secrete into the maternal circulation a family of proteins known as pregnancy specific glycoproteins (PSG). The current study was undertaken to characterize the receptor for two members of the murine PSG family, PSG17 and PSG23. Binding of recombinant PSG17 and...

    journal_title:Placenta

    pub_type: 杂志文章

    doi:10.1016/j.placenta.2011.05.008

    authors: Sulkowski GN,Warren J,Ha CT,Dveksler GS

    更新日期:2011-08-01 00:00:00

  • Non-selective cationic channels (NSCC) in smooth muscle cells from human umbilical arteries.

    abstract::The aim of our work was to investigate the presence of non-selective cation channels (NSCC) in freshly isolated smooth muscle cells from the human umbilical artery (HUA), one of the vessels involved in fetal-placental circulation. We studied the electrophysiological properties of NSCC using the patch-clamp technique i...

    journal_title:Placenta

    pub_type: 杂志文章

    doi:10.1016/j.placenta.2004.01.028

    authors: Raingo J,Rebolledo A,Iveli F,Grassi de Gende AO,Milesi V

    更新日期:2004-09-01 00:00:00

  • Membrane potential and input resistance in syncytiotrophoblast of human term placenta in vitro.

    abstract::Direct measurements of electrical potential and input resistance were performed on the syncytial membrane facing maternal blood. The potentials varied according to the external potassium ion levels and, to a lesser extent, with sodium ion concentrations. Ouabain provoked a rapid and consistent depolarization which ind...

    journal_title:Placenta

    pub_type: 杂志文章

    doi:10.1016/0143-4004(88)90012-4

    authors: Bara M,Challier JC,Guiet-Bara A

    更新日期:1988-03-01 00:00:00

  • Altered gene expression of H19 and IGF2 in placentas from ART pregnancies.

    abstract:INTRODUCTION:The aim of this study is to determine whether the gene expression and associated DNA methylation regulation of H19 and IGF2 are altered in placentas conceived by assisted reproductive technologies (ART) compared to natural conceptions. METHODS:113 pregnancies were recruited resulting in 119 placentas (83 ...

    journal_title:Placenta

    pub_type: 杂志文章

    doi:10.1016/j.placenta.2015.08.008

    authors: Sakian S,Louie K,Wong EC,Havelock J,Kashyap S,Rowe T,Taylor B,Ma S

    更新日期:2015-10-01 00:00:00

  • Human placenta: a human organ for developmental toxicology research and biomonitoring.

    abstract::Pregnant mothers are exposed to a wide variety of foreign chemicals. This exposure is most commonly due to maternal medication, lifestyle factors, such as smoking, drug abuse, and alcohol consumption, or occupational and environmental sources. Foreign compounds may interfere with placental functions at many levels e.g...

    journal_title:Placenta

    pub_type: 杂志文章,评审

    doi:10.1016/j.placenta.2004.09.006

    authors: Myllynen P,Pasanen M,Pelkonen O

    更新日期:2005-05-01 00:00:00

  • Placental function and structure at term is altered in broodmares fed with cereals from mid-gestation.

    abstract:INTRODUCTION:Feeding pregnant broodmares with cereal concentrates has been shown to increase maternal insulin resistance and affect foal metabolism in the short and long-term. These effects are likely to be mediated by the placenta. Here, we investigated feto-placental biometry and placental structure and function at t...

    journal_title:Placenta

    pub_type: 杂志文章

    doi:10.1016/j.placenta.2018.02.003

    authors: Robles M,Peugnet P,Dubois C,Piumi F,Jouneau L,Bouchez O,Aubrière MC,Dahirel M,Aioun J,Wimel L,Couturier-Tarrade A,Chavatte-Palmer P

    更新日期:2018-04-01 00:00:00

  • Downregulation of HLA class I gene transcription in choriocarcinoma cells is controlled by the proximal promoter element and can be reversed by CIITA.

    abstract::The expression pattern of MHC class I genes in trophoblast cells at the feto-maternal interface is thought to be the basis of the maintenance of pregnancy by protecting the fetus from maternal immune rejection. Transcription of classical HLA class I genes is low or undetectable in most trophoblast cells as well as in ...

    journal_title:Placenta

    pub_type: 杂志文章

    doi:10.1053/plac.1998.0380

    authors: Lefebvre S,Moreau P,Dausset J,Carosella ED,Paul P

    更新日期:1999-05-01 00:00:00

  • Differential host response to LPS variants in amniochorion and the TLR4/MD-2 system in Macaca nemestrina.

    abstract:OBJECTIVES:Microbial-specific factors are likely critical in determining whether bacteria trigger preterm labor. Structural variations in lipopolysaccharide (LPS), a component of gram-negative bacteria, can determine whether LPS has an inflammatory (agonist) or anti-inflammatory (antagonist) effect through Toll-like re...

    journal_title:Placenta

    pub_type: 杂志文章

    doi:10.1016/j.placenta.2010.06.010

    authors: Chang J,Jain S,Carl DJ,Paolella L,Darveau RP,Gravett MG,Adams Waldorf KM

    更新日期:2010-09-01 00:00:00

  • Maternal high-fat diet sex-specifically alters placental morphology and transcriptome in rats: Assessment by next-generation sequencing.

    abstract:INTRODUCTION:Maternal nutrition is an extremely important health issue. We evaluated the impact of maternal high fat diet (HFD) on pregnancy outcomes, elucidated how the rat placenta and fetus respond to diet manipulation based on fetal sex, and identified candidate genes and pathways. METHODS:Rats were fed a normal o...

    journal_title:Placenta

    pub_type: 杂志文章

    doi:10.1016/j.placenta.2019.03.004

    authors: Lin YJ,Huang LT,Tsai CC,Sheen JM,Tiao MM,Yu HR,Lin IC,Tain YL

    更新日期:2019-03-01 00:00:00

  • Classics revisited: Dietrich Starck on comparative embryology and placentation.

    abstract:BACKGROUND:Dietrich Starck (1908-2001) was a German embryologist who wrote extensive reviews on comparative placentation. FINDINGS:Starck's embryology textbook and his comprehensive review of comparative embryology and placentation give excellent insights into the foundational literature and are extensively referenced...

    journal_title:Placenta

    pub_type: 传,历史文章,杂志文章

    doi:10.1016/j.placenta.2017.08.073

    authors: Carter AM

    更新日期:2017-10-01 00:00:00

  • Trophoblast deportation part II: a review of the maternal consequences of trophoblast deportation.

    abstract::The deportation of trophoblast debris from the placenta was first documented over 100 years ago, and today we know that the deported material ranges from multinucleated syncytial knots/sprouts to trophoblast-derived nanoparticles. However little is known about the effect of trophoblast debris on maternal physiology si...

    journal_title:Placenta

    pub_type: 杂志文章,评审

    doi:10.1016/j.placenta.2011.06.019

    authors: Pantham P,Askelund KJ,Chamley LW

    更新日期:2011-10-01 00:00:00

  • Endoplasmic reticulum stress is induced in the human placenta during labour.

    abstract::Placental endoplasmic reticulum (ER) stress has been postulated in the pathophysiology of pre-eclampsia (PE) and intrauterine growth restriction (IUGR), but its activation remains elusive. Oxidative stress induced by ischaemia/hypoxia-reoxygenation activates ER stress in vitro. Here, we explored whether exposure to la...

    journal_title:Placenta

    pub_type: 杂志文章

    doi:10.1016/j.placenta.2014.11.005

    authors: Veerbeek JH,Tissot Van Patot MC,Burton GJ,Yung HW

    更新日期:2015-01-01 00:00:00