Abstract:
:Chromatin posttranslational modifications (PTMs), including monoubiquitylation of histone H2B on lysine 120 (H2Bub1), play a major role in regulating genome functions. To elucidate the molecular mechanisms of H2Bub1 activity, a chromatin template uniformly containing H2Bub1 was used as an affinity matrix to identify preferentially interacting human proteins. Over 90 such factors were found, including proteins and protein complexes associated with transcription, RNA posttranscriptional modifications, and DNA replication and repair. Notably, we found that the SWI/SNF chromatin remodeling complex associates preferentially with H2Bub1-rich chromatin. Moreover, SWI/SNF is required for optimal transcription of a subset of genes that are selectively dependent on H2Bub1. Our findings substantially expand the known H2Bub1 interactome and provide insights into the functions of this PTM in mammalian gene regulation.
journal_name
Cell Repjournal_title
Cell reportsauthors
Shema-Yaacoby E,Nikolov M,Haj-Yahya M,Siman P,Allemand E,Yamaguchi Y,Muchardt C,Urlaub H,Brik A,Oren M,Fischle Wdoi
10.1016/j.celrep.2013.07.014subject
Has Abstractpub_date
2013-08-15 00:00:00pages
601-8issue
3issn
2211-1247pii
S2211-1247(13)00361-6journal_volume
4pub_type
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