Abstract：
:It is currently assumed that 3D chromosomal organization plays a central role in transcriptional control. However, depletion of cohesin and CTCF affects the steady-state levels of only a minority of transcripts. Here, we use high-resolution Capture Hi-C to interrogate the dynamics of chromosomal contacts of all annotated human gene promoters upon degradation of cohesin and CTCF. We show that a majority of promoter-anchored contacts are lost in these conditions, but many contacts with distinct properties are maintained, and some new ones are gained. The rewiring of contacts between promoters and active enhancers upon cohesin degradation associates with rapid changes in target gene transcription as detected by SLAM sequencing (SLAM-seq). These results provide a mechanistic explanation for the limited, but consistent, effects of cohesin and CTCF depletion on steady-state transcription and suggest the existence of both cohesin-dependent and -independent mechanisms of enhancer-promoter pairing.
journal_name
Cell Repjournal_title
Cell reportsauthors
Thiecke MJ,Wutz G,Muhar M,Tang W,Bevan S,Malysheva V,Stocsits R,Neumann T,Zuber J,Fraser P,Schoenfelder S,Peters JM,Spivakov Mdoi
10.1016/j.celrep.2020.107929subject
Has Abstractpub_date
2020-07-21 00:00:00pages
107929issue
3issn
2211-1247pii
S2211-1247(20)30910-4journal_volume
32pub_type
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