Abstract：
:Nogo-A has been identified as an inhibitory molecule to neurite outgrowth after injury in adult mammalian central nervous system (CNS). The C-terminal fragment of Nogo-A, Nogo-66, inhibits axonal regrowth through NgR1 signaling. Residues 1-32 of Nogo-66 cover two regions that contribute most affinity of Nogo-66 to NgR1. It is unclear whether blocking the two regions with specific small ligands could neutralize the inhibition of Nogo-66. Therefore in this study we explored two phage display peptide libraries to screen small peptides that might bind Nogo-66. NEP1-35 containing 1-33 residues of Nogo-66 was taken as the target for panning. We found that phage-borne peptides with stronger affinity to NEP1-35 contained a relatively conserved motif, RRXXXXXXXRRX. Afterwards one identified peptide, NH(2)-RRQTLSHQMRRP-COOH was synthesized and tested in neurite outgrowth assay, in which this small molecule showed moderate ability to neutralize CNS myelin inhibition in vitro. Our results demonstrated that short peptides could act as adaptors to Nogo-66 and neutralize CNS myelin inhibition in vitro. Additionally, the results also suggested that phage display could help to discover novel small molecules with high affinity to CNS regrowth inhibitors, which might be able to promote CNS regeneration with fewer side effects since they could block only the corresponding regions of inhibitors.
journal_name
Neurosci Lettjournal_title
Neuroscience lettersauthors
Deng Q,Cai W,Li S,Su Bdoi
10.1016/j.neulet.2013.01.009subject
Has Abstractpub_date
2013-03-01 00:00:00pages
80-4eissn
0304-3940issn
1872-7972pii
S0304-3940(13)00029-3journal_volume
536pub_type
杂志文章abstract：:The role of intracellular amyloid beta (iAbeta) in Alzheimer's disease (AD) initiation and progression attracts more and more attention in recent years. To address whether iAbeta induces early alterations of electrophysiological properties in cultured human primary neurons, we delivered iAbeta with adeno-virus and mea...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2009.07.031
更新日期：2009-10-25 00:00:00
abstract：:Mechanosensitivity is a crucial but poorly understood property of the sensory nervous system. Transient receptor potential (TRP) channels, which have been found to be responsible for the detection of other sensory stimuli such as temperature and pungent chemicals, have been suggested to also recognize stretch or press...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2009.03.082
更新日期：2009-06-19 00:00:00
abstract：:The specific phosphatase inhibitor, Calyculin-A (CL-A), decreases high-K stimulated catecholamine secretion in bovine chromaffin cells. This effect can be split into two components: one needs long exposures to the drug to be elicited, and is sensitive to the protein kinase-inhibitor K252a; the other is observed after ...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/0304-3940(94)90288-7
更新日期：1994-08-29 00:00:00
abstract：:By recording the locomotor activity rhythm of hagfish, Eptatretus burgeri, in which surgical lesions of the pretectal areas were made, we searched for the route of light information from the eyes to the circadian pacemaker, which is considered to be located in the preoptic nucleus. The entrainment of circadian activit...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/0304-3940(95)11645-d
更新日期：1995-06-16 00:00:00
abstract：:By studying the binding of [3H]glycine and [3H]glutamate to rat synaptic membranes in the presence of 2-amino-5-phosphonovalerate (APV) and kynurenate (KYN) we have demonstrated that KYN is more potent than APV in displacing [3H]glycine, while an opposite order of potency was seen in displacing [3H]glutamate. Moreover...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/0304-3940(89)90402-3
更新日期：1989-01-30 00:00:00
abstract：:Recently, there has been a lot of interest in the neuroimaging community in exploring fMRI time-series measures of local neuronal activity and excitation/inhibition (E/I) balance in the brain. In this preliminary study we probed the sensitivity of widely used sample entropy (SE) measure at multiple scales to controlle...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2020.134984
更新日期：2020-05-29 00:00:00
abstract：:The effect of lesions on nerve growth factor receptor (NGFr) immunoreactivity (IR) in motoneurons within the mature feline trigeminal motor nucleus was investigated. Ten days following complete unilateral retrogasserian trigeminal rhizotomy including transection of the trigeminal motor root, motoneurons within the ips...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/0304-3940(91)90540-a
更新日期：1991-05-27 00:00:00
abstract：:Previously, we have demonstrated that pressure-ejected application of angiotensin II and losartan, an angiotensin AT1 receptor antagonist, onto some neurons in the anterior hypothalamic area (AHA) of the rat increases and decreases, respectively, the basal firing rate of the neurons. To investigate possible participat...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2007.01.059
更新日期：2007-04-12 00:00:00
abstract：:A 12h sleep deprivation enhances the expression of 5-hydroxytryptamine (5-HT) 1A receptors in rat hippocampus that recedes with 48h sleep recovery. The depressant effect of applied 5-HT on the field excitatory postsynaptic potentials recorded in the CA1 area, is also enhanced in hippocampi of SD rats. Following a 24 o...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2017.06.053
更新日期：2017-08-10 00:00:00
abstract：:Using a training protocol that effectively induces procedural memory consolidation (PMC) in young adults, we show that older adults are good learners, robustly improving their motor performance during training. However, performance declined over the day, and overnight 'offline' consolidation phase performance gains we...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2015.08.051
更新日期：2015-10-08 00:00:00
abstract：:Intolerance of uncertainty (IU), or the increased affective response to situations with uncertain outcomes, is an important component process of anxiety disorders. Increased IU is observed in panic disorder (PD), obsessive compulsive disorder (OCD) and generalized anxiety disorder (GAD), and is thought to relate to dy...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2007.10.030
更新日期：2008-01-10 00:00:00
abstract：:Neuroglobin (Ngb) is a novel vertebrate globin expressed principally in neurons. Ngb expression is induced by hypoxia and ischemia, and Ngb protects neurons against these insults. The mechanism of Ngb's protective action is unknown, but its ability to bind NO suggests that NO scavenging might be involved. To test this...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2007.10.031
更新日期：2008-01-10 00:00:00
abstract：:The homozygous Brattleboro rat (di/di) synthesizes a vasopressin (VP) precursor with an aberrant C-terminus, which causes a hypothalamic form of diabetes insipidus. The neuroendocrine polypeptide 7B2 is present in VP and oxytocin (OT) neurons of the supraoptic and paraventricular nucleus of the hypothalamus in wild ty...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/s0304-3940(97)00883-5
更新日期：1997-12-12 00:00:00
abstract：OBJECT:Much evidence has demonstrated that stress and tumor interact, but the mechanisms are poorly understood. The purpose of this study is to discuss the effect of unpredictable chronic mild stress (CMS) upon the behavior of Walker 256 tumor-bearing rats and its mechanism. METHODS:Observe the effects of CMS on the s...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2009.06.031
更新日期：2010-03-31 00:00:00
abstract：:Rat neocortical slices maintained in Mg2(+)-free Krebs medium developed spontaneous paroxysmal discharges which were attenuated or suppressed by the gamma-aminobutyric acid-B (GABAB) receptor agonist baclofen, occasionally accompanied by a slight hyperpolarisation, and antagonised by the specific GABAB-receptor antago...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/0304-3940(90)90557-p
更新日期：1990-02-05 00:00:00
abstract：:Following unilateral transection of the medial forebrain bundle (MFB) within the anterior hypothalamic-preoptic region of cats, the biting attack upon a rat elicited by ipsilateral posterior hypothalamic or ventral midbrain stimulation is eliminated, although the cat continues to approach from 2.8 metres away to withi...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/0304-3940(81)90387-6
更新日期：1981-01-20 00:00:00
abstract：:Normal-learning children (NL) and children with learning disabilities (LD) reported their perceptions of unisensory (auditory or visual), concordant audiovisual (e.g. visual /apa/ and auditory /apa/) and conflicting (e.g. visual /aka/ and auditory /apa/) speech stimuli in quiet and noise (0 dB and -12 dB signal-to-noi...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/s0304-3940(03)00971-6
更新日期：2003-11-06 00:00:00
abstract：:To determine whether axonal terminals emanating from the central nucleus of amygdala (Ce) to the parabrachial nucleus (PBN) contain gamma-aminobutyric acid (GABA) as their neurotransmitter, an electron microscopic study was performed employing the combined techniques of WGA-HRP anterograde tracing and post-embedding i...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2005.03.013
更新日期：2005-07-01 00:00:00
abstract：:Acetylcholine, synthesized in the cytoplasm of cholinergic neurons by choline acetyltransferase (ChAT), is packaged in synaptic vesicles by vesicular acetylcholine transporter (VAChT). The entire VAChT gene has been reported to be located within the first intron of the ChAT gene. In order to examine whether or not ChA...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/s0304-3940(97)00763-5
更新日期：1997-10-31 00:00:00
abstract：:We have previously reported that there is an up-regulation of the NR2B gene expression in the adult cortex and cultured fetal cortical neurons of mice following chronic ethanol treatment due to demethylation of cytosine residues in the NR2B gene CpG island. In the present study, we investigated the methylation pattern...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2005.01.042
更新日期：2005-06-03 00:00:00
abstract：:Expression levels of amyloid beta (Abeta)-degrading enzymes, insulin degrading enzyme (IDE) and neprilysin (NEP), were examined in transgenic mice with Alzheimer's disease-like neuropathology. After the development of first Abeta plaques in transgenic mice brain, cortical mRNA and protein levels of IDE were significan...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2008.04.025
更新日期：2008-06-20 00:00:00
abstract：:Spontaneous gasps were recorded in pentobarbitone-anesthetized adult cats. Mean interval between gasps in 11 cats with their buffer nerves intact was of 65 sec; it was significantly prolonged to 174 sec after unilateral carotid neurotomy and to 403 sec after bilateral carotid neurotomy. Additional sectioning of both a...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/0304-3940(80)90110-x
更新日期：1980-01-01 00:00:00
abstract：:Previously we have shown that the 6-sulphatoxymelatonin rhythm of oil rig workers on a 2-week night shift (1800-0600 h) adapts to the shift via a phase delay. We now report the findings of a study on two offshore drill crews working a 1 week day (1200-0000 h), 1 week night (0000-1200 h) swing shift. Urine samples were...
journal_title：Neuroscience letters
pub_type： 临床试验,杂志文章
doi：10.1016/s0304-3940(98)00585-0
更新日期：1998-08-21 00:00:00
abstract：:The retinohypothalamic tract (RHT) in the Djungarian hamster Phodopus sungorus was studied using anterograde neuronal transport of cholera toxin subunit B (CTB) following unilateral intraocular injection. A major projection terminates bilaterally in the hypothalamic suprachiasmatic nuclei (SCN). In the anterior ventra...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/0304-3940(94)90116-3
更新日期：1994-06-06 00:00:00
abstract：:Morphine blocked decremental bar pressing of rats to escape electrical stimulation of aversive midbrain sites. These sites included the dorsal central gray area (DCG), the ventral reticular formation (VRF) and the lateral tegmentum (LT). Naloxone, which had no effect alone, blocked the morphine effects of all three av...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/0304-3940(78)90035-6
更新日期：1978-11-01 00:00:00
abstract：:Effects of anxiety on perceptual-motor organisation of rhythmical forearm movements were examined using an interrupted time series design with staggered baselines. Participants were exposed to repeated baseline sessions interrupted with two anxiety-inducing sessions. Results showed that under moderate levels of anxiet...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2005.04.036
更新日期：2005-08-12 00:00:00
abstract：:Tumor necrosis factor-alpha (TNF-alpha) is a proinflammatory cytokine involved in the development and maintenance of inflammatory and neuropathic pain conditions. The mechanisms by which TNF-alpha elicits pain behavior are still incompletely understood. Numerous studies suggest that TNF-alpha sensitizes primary affere...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2008.01.070
更新日期：2008-04-04 00:00:00
abstract：:Brain-derived neurotrophic factor (BDNF) has been proposed as a key regulator and mediator of long-term synaptic modifications related to learning and memory maintenance. Our previous studies show that application of high-frequency stimulation (HFS) sufficient to elicit LTP at the dentate gyrus (DG)-CA3 pathway produc...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2008.08.069
更新日期：2008-11-07 00:00:00
abstract：:The results of several studies have suggested that local circuit neurons, or interneurons, of area CA1 of hippocampus use gamma-aminobutyric acid (GABA) as their neurotransmitter. However, when these cells were labelled by intracellular dye injection, and examined immunocytochemically with antisera raised against GABA...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/0304-3940(89)90237-1
更新日期：1989-01-02 00:00:00
abstract：:Preterm infants undergo several painful procedures during their stay in neonatal intensive care units. Previous studies suggest that early painful experiences may have an impact on brain development. Here, we used an animal model to investigate the effect of neonatal pain on the generation of new neurons in the dentat...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2011.06.047
更新日期：2011-08-26 00:00:00
