Abstract：
:Mechanosensitivity is a crucial but poorly understood property of the sensory nervous system. Transient receptor potential (TRP) channels, which have been found to be responsible for the detection of other sensory stimuli such as temperature and pungent chemicals, have been suggested to also recognize stretch or pressure to cell membranes. TRPC1 is one candidate from studies in oocytes but evidence in native sensory neurons has been lacking. Therefore, we have measured an increase in intracellular Ca(2+) levels upon mechanical activation of native mouse dorsal root ganglion (DRG) neurons in culture using hypoosmolar buffer. Our results show that down regulation of TRPC1 with short hairpin RNA results in a 65% reduction of neurons with stretch activated responses. These results implicate a direct or indirect involvement of TRPC1 in the mechanosensitivity of DRG neurons.
journal_name
Neurosci Lettjournal_title
Neuroscience lettersauthors
Staaf S,Maxvall I,Lind U,Husmark J,Mattsson JP,Ernfors P,Pierrou Sdoi
10.1016/j.neulet.2009.03.082subject
Has Abstractpub_date
2009-06-19 00:00:00pages
3-7issue
1eissn
0304-3940issn
1872-7972pii
S0304-3940(09)00397-8journal_volume
457pub_type
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