A GABAergic projection from the central nucleus of the amygdala to the parabrachial nucleus: an ultrastructural study of anterograde tracing in combination with post-embedding immunocytochemistry in the rat.

Abstract:

:To determine whether axonal terminals emanating from the central nucleus of amygdala (Ce) to the parabrachial nucleus (PBN) contain gamma-aminobutyric acid (GABA) as their neurotransmitter, an electron microscopic study was performed employing the combined techniques of WGA-HRP anterograde tracing and post-embedding immunocytochemistry for GABA. Our analysis distinguished a large population of GABA immunopositive axonal terminals from the Ce that exhibited symmetrical synaptic contacts with neurons in the lateral parabrachial nucleus. Additionally, most retrogradely labeled dendrites and perikarya received synaptic contacts from GABA immunoreactive terminals, with some of them originating from the Ce. The present study provides the first direct ultrastructural evidence for a monosynaptic, GABAergic link between Ce axons and neurons of the parabrachial nucleus via classical symmetrical synapses.

journal_name

Neurosci Lett

journal_title

Neuroscience letters

authors

Jia HG,Zhang GY,Wan Q

doi

10.1016/j.neulet.2005.03.013

keywords:

subject

Has Abstract

pub_date

2005-07-01 00:00:00

pages

153-7

issue

1-2

eissn

0304-3940

issn

1872-7972

pii

S0304-3940(05)00304-6

journal_volume

382

pub_type

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