Abstract:
:The mental retardation-associated protein, srGAP3 is highly expressed in neurogenic sites. It is thought to regulate the key aspects of neuronal development and functions. Little is known about the interaction between srGAP3 and immature neural stem cells/neural progenitor cells (NSCs/NPCs). In the current study, the expression of srGAP3 in NSCs/NPCs was detected. Then, survival, proliferation, differentiation, and morphological alteration of NSCs/NPCs were assessed after a lentivirus-mediated knockdown of srGAP3. The results showed that srGAP3 is highly expressed in NSCs/NPCs both in vitro and in vivo. After knockdown of srGAP3 (LV3-srGAP3 infection), viability and proliferation of NSCs/NPCs dramatically decreased, approximately 85% displayed a similar morphology with type I cells that have no or only few indistinguishable processes. After 7 days culture in a differentiation medium, 62.5%±8.3% of cells in the srGAP3 knockdown group were nestin-positive and 24.8%±5.8% of them were β-tubulin III-positive, which are significantly higher (30.2%±9.9% and 14.6%±2.7%) than in the control group (LV3-NC infection). In addition, cells in the knockdown group had significantly fewer, but longer processes. Our results demonstrate that srGAP3 knockdown negatively regulates NSCs/NPCs survival, proliferation, differentiation, and morphological alteration, particularly, process formation. Taken together, our results provide strong evidence that srGAP3 is involved in the regulation of biological behavior and the morphological features in rat NSCs/NPCs in vitro.
journal_name
Stem Cells Devjournal_title
Stem cells and developmentauthors
Lu H,Jiao Q,Wang Y,Yang Z,Feng M,Wang L,Chen X,Jin W,Liu Ydoi
10.1089/scd.2012.0455subject
Has Abstractpub_date
2013-06-01 00:00:00pages
1709-16issue
11eissn
1547-3287issn
1557-8534journal_volume
22pub_type
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journal_title:Stem cells and development
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journal_title:Stem cells and development
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journal_title:Stem cells and development
pub_type: 杂志文章,评审
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journal_title:Stem cells and development
pub_type: 杂志文章
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journal_title:Stem cells and development
pub_type: 杂志文章,评审
doi:10.1089/scd.2015.0008
更新日期:2015-09-15 00:00:00
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journal_title:Stem cells and development
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doi:10.1089/scd.2011.0194
更新日期:2012-01-20 00:00:00
abstract::Over the past 20 months, reports claiming the generation of induced pluripotent stem (iPS) cells with characteristics identical to those of embryonic stem (ES) cells from nonembryonic tissue have captured great attention in both the scientific community and the general public. In the light of the continuing controvers...
journal_title:Stem cells and development
pub_type: 杂志文章,评审
doi:10.1089/scd.2008.0062
更新日期:2008-06-01 00:00:00
abstract::Hepatic ischemia reperfusion injury (IRI) remains a major obstacle in liver resection and transplantation surgery, especially in diseased organs. Human mesenchymal stromal cells (MSCs) are reported to acutely alleviate hepatic IRI in mice by releasing bioactive membrane-enclosed extracellular vesicles (EVs), but the l...
journal_title:Stem cells and development
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doi:10.1089/scd.2019.0085
更新日期:2019-11-01 00:00:00
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journal_title:Stem cells and development
pub_type: 杂志文章,评审
doi:10.1089/scd.2012.0373
更新日期:2013-01-15 00:00:00
abstract::Betacellulin (BTC), a ligand of the epidermal growth factor receptor, has been shown to promote growth and differentiation of pancreatic β-cells and to improve glucose metabolism in experimental diabetic rodent models. Mesenchymal stem cells (MSCs) have been already proved to be multipotent. Recent work has attributed...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2009.0490
更新日期:2011-02-01 00:00:00
abstract::Ossification of the posterior longitudinal ligament (OPLL) is characterized by ectopic OPLL. To date, the specific molecular pathogenesis of OPLL has not been clearly elucidated. In this study, bone marrow-derived mesenchymal stem cells obtained from healthy donors (HD-MSCs) and patients with OPLL (OPLL-MSCs) were cul...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2020.0117
更新日期:2020-12-01 00:00:00
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journal_title:Stem cells and development
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doi:10.1089/scd.2016.0061
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abstract::Embryonic stem (ES) cells give rise to mesodermal progenitors that differentiate to hematopoietic and cardiovascular cells. The wnt signaling pathway plays multiple roles in cardiovascular development through a network of intracellular effectors. To monitor global changes in wnt signaling during ES cell differentiatio...
journal_title:Stem cells and development
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journal_title:Stem cells and development
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doi:10.1089/scd.2009.0020
更新日期:2009-11-01 00:00:00
abstract::Human embryonic stem cells (hESCs) are derived from the inner cell mass (ICM) of the blastocyst and can differentiate into any cell type in the human body. These cells hold a great potential for regenerative medicine, but to obtain enough cells needed for medical treatment, culture is required on a large scale. In the...
journal_title:Stem cells and development
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abstract::One of the challenges in stem cell research is to avoid transformation during cultivation. We studied high passage subventricular zone derived neural stem cells (NSCs) cultures of adult rats in the absence of growth factors epidermal growth factor (EGF) and basic fibroblast growth factor (bFGF). We termed this culture...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2009.0416
更新日期:2010-07-01 00:00:00
abstract::Multipotent mesenchymal stem/stromal cells (MSCs) are strongly involved in tissue homeostasis mainly through paracrine regulation. In this study, we examined the influence of simulated microgravity on the angiogenic potential of adipose-derived MSCs (ASCs). The conditioned medium (CM) from random positioning machine (...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2017.0262
更新日期:2018-06-15 00:00:00
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journal_title:Stem cells and development
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abstract::In this study we examine whether a somatic cell, once returned to a pluripotent state, gains the ability to reprogram other somatic cells. We reprogrammed mouse embryonic fibroblasts by viral induction of oct4, sox2, c-myc, and klf-4 genes. Upon fusion of the resulting iPS cells with somatic cells harboring an Oct4-GF...
journal_title:Stem cells and development
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abstract::Bone marrow-mesenchymal stem cells (BM-MSC) ameliorate renal dysfunction and repair tubular damage of acute kidney injury by locally releasing growth factors, including the insulin-like growth factor-1 (IGF-1). The restricted homing of BM-MSC at the site of injury led us to investigate a possible gene-based communicat...
journal_title:Stem cells and development
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abstract::Previous studies have transplanted a variety of neural stem cells (NSCs) to the eye in hopes of developing a therapy to replace retinal neurons lost to disease. Successful integration, survival, and differentiation of the cell types has been variably successful. At the moment, little is known about the fundamental bio...
journal_title:Stem cells and development
pub_type: 杂志文章
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abstract::Retinoic acid (RA) is present at sites of neurogenesis in both the embryonic and adult brain. While it is widely accepted that RA signaling is involved in the regulation of neural stem cell differentiation, little is known about vitamin A utilization and biosynthesis of active retinoids in the neurogenic niches, or ab...
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