Abstract:
:Over the past 20 months, reports claiming the generation of induced pluripotent stem (iPS) cells with characteristics identical to those of embryonic stem (ES) cells from nonembryonic tissue have captured great attention in both the scientific community and the general public. In the light of the continuing controversy over the use of ES cells, these reports have profound ramifications. This review calls into question the validity of many claims made in these reports--claims that have led to the rapid and premature acceptance of using iPS cells as a viable alternative to using normal stem cells for regenerative therapy. How convincing is the evidence supporting the various claims made for the iPS cells? Are there other more plausible explanations for the same observations? What are these iPS cells? Are they really safe for therapeutic use? Should the iPS technique be considered, in the absence of any direct evidence for induction and reprogramming, as a realistic alternative for somatic cell nuclear transfer (SCNT) to generate ES-like cells? This review attempts to encourage reflections on and offer alternative views for key aspects of iPS cells and studies.
journal_name
Stem Cells Devjournal_title
Stem cells and developmentauthors
Liu SVdoi
10.1089/scd.2008.0062subject
Has Abstractpub_date
2008-06-01 00:00:00pages
391-7issue
3eissn
1547-3287issn
1557-8534journal_volume
17pub_type
杂志文章,评审abstract::A long-term goal of mesenchymal progenitor cell (MPC) research is to identify cell-surface markers to facilitate MPC isolation. One reported MPC feature in humans and other species is lack of CD14 (lipopolysaccharide receptor) expression. The aim of this study was to evaluate CD14 as an MPC sorting marker. Our hypothe...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2010.0175
更新日期:2011-04-01 00:00:00
abstract::To develop biological approaches to restore vision, we developed a method of transplanting stem cell-derived retinal tissue into the subretinal space of a large-eye animal model (cat). Human embryonic stem cells (hESC) were differentiated to retinal organoids in a dish. hESC-derived retinal tissue was introduced into ...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2019.0090
更新日期:2019-09-01 00:00:00
abstract::Myocardial infarction is a major public health problem that causes significant mortality despite recent advances in its prevention and treatment. Therefore, approaches based on adult stem cells represent a promising alternative to conventional therapies for this life-threatening condition. Mesenchymal stem cells (MSCs...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2008.0292
更新日期:2009-07-01 00:00:00
abstract::How hematopoietic stem cells (HSCs) maintain the balance of self-renewal and differentiation could be partially ascribed to asymmetric and symmetric division patterns. However, a simple and effective method to detect stem cell division patterns is lacking. In this study, we introduce a strategy to describe stem cells ...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2018.0239
更新日期:2019-07-15 00:00:00
abstract::In a previous study we established colony assays suitable for studying murine adult (2-4 months) pancreatic progenitor cells plated in semisolid media containing methylcellulose and extracellular matrix proteins. Using these assays, we found robust in vitro progenitor cell activities (multilineage differentiation and ...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2013.0466
更新日期:2014-04-15 00:00:00
abstract::Periodontium is consisted of root cementum, bone lining the tooth socket, gingiva facing the tooth, and periodontal ligament (PDL). Its primary functions are support of the tooth and protection of tooth, nerve, and blood vessels from injury by mechanical loading. Severe periodontitis induces the destruction of periodo...
journal_title:Stem cells and development
pub_type: 杂志文章,评审
doi:10.1089/scd.2019.0031
更新日期:2019-08-01 00:00:00
abstract::Periodontitis is an infectious disease characterized by chronic inflammation and progressive destruction of periodontal tissues. Chronic inflammatory environment may affect immunomodulatory function of periodontal ligament stem cells (PDLSCs) and promote shift toward proinflammatory phenotype contributing to propagati...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2019.0021
更新日期:2019-08-01 00:00:00
abstract::Human embryonic stem (hES) cells represent a potentially unlimited source of transplantable beta-cells for the treatment of diabetes. Here we describe a differentiation strategy that reproducibly directs HES3, an National Institutes of Health (NIH)-registered hES cell line, into cells of the pancreatic endocrine linea...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2007.0029
更新日期:2007-08-01 00:00:00
abstract::Mesenchymal stem cells (MSCs) from bone marrow (BM) are widely used for bone and less for cartilage tissue regeneration due to their self-renewal and differentiating properties into osteogenic or chondrogenic lineages. This review considers the last decade of clinical trials involving a two-step procedure, by expandin...
journal_title:Stem cells and development
pub_type: 杂志文章,评审
doi:10.1089/scd.2012.0373
更新日期:2013-01-15 00:00:00
abstract::Dental follicle stem cells (DFSCs), which are the stem cells present in the dental follicle tissue of the tooth germ and are derived from the neural crest, are direct precursor cells of periodontal tissues and can form the periodontal ligament, cementum, and alveolar bone proper in the late stage of tooth development....
journal_title:Stem cells and development
pub_type: 杂志文章,评审
doi:10.1089/scd.2019.0012
更新日期:2019-08-01 00:00:00
abstract::Mesenchymal stem cells (MSCs) can be mobilized from the bone marrow and enter the circulation. Conversely, MSCs can be recruited from the circulation and into the bone marrow. For these migratory pathways, MSCs have to traverse the bone marrow endothelium, in a basal-to-apical and apical-to-basal direction, respective...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2011.0025
更新日期:2012-02-10 00:00:00
abstract::Primordial germ cells (PGCs), the precursors of gametes, have regulatory mechanisms involving transcription factors and epigenetic modifications. The transcription factor NANOG is a key regulator of germ cell and embryonic stem cell characteristics. However, the epigenetic regulation of NANOG with a histone deacetylas...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2018.0059
更新日期:2018-09-01 00:00:00
abstract::Acute respiratory distress syndrome (ARDS)/acute lung injury (ALI) is a severe clinical respiratory failure disorder associated with chronic pathology and disability and has a mortality rate of 40%-60%. However, the pathogenesis of ARDS/ALI remains unclear, and existing therapeutic options are insufficient for address...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2020.0174
更新日期:2021-02-01 00:00:00
abstract::Allergic asthma is an inflammatory airway disease caused by T helper type 2 (Th2)-driven immune responses. Recent studies have demonstrated that adipose-derived stromal cells (ASC) have an immunosuppressive effect on T-cell activity. This study was performed to investigate whether ASC can inhibit Th2-dependent allergi...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2009.0513
更新日期:2010-11-01 00:00:00
abstract::Cardiac stem/progenitors are being used in the clinic to treat patients with a range of cardiac pathologies. However, improvements in heart function following treatment have been reported to be variable, with some showing no response. This discrepancy in response remains unresolved. Mesenchymal stem cells (MSCs) have ...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2018.0170
更新日期:2019-05-01 00:00:00
abstract::Somatic cells can be reprogrammed to induced pluripotent stem cells (iPSCs) by defined factors. However, substantial cell numbers subjected to iPSC induction stray from the main reprogramming route and are immortalized as partial iPSCs. These partial iPSCs can become genuine iPSCs by exposure to the ground state condi...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2013.0326
更新日期:2014-09-15 00:00:00
abstract::Red blood cell (RBC) differentiation from human induced pluripotent stem cells (hiPSCs) offers great potential for developmental studies and innovative therapies. However, ex vivo erythropoiesis from hiPSCs is currently limited by low efficiency and unphysiological conditions of common culture systems. Especially, the...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2019.0132
更新日期:2019-12-01 00:00:00
abstract::Human perivascular progenitor cells, including pericytes, are well-described multipotent mesenchymal cells giving rise to mesenchymal stem cells in culture. Despite the unique location of pericytes, specific antigens to distinguish human pericytes from other cell types are few. Here, we employed a human tissue microar...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2019.0106
更新日期:2019-09-15 00:00:00
abstract::To be clinically relevant as a therapy for heart failure, endogenous progenitor cells must be isolated and expanded from aged and/or diseased tissue. Here, we investigated the effect of age and cardiac impairment resulting from lack of dystrophin on murine cardiosphere-derived cells (CDCs). CDCs were isolated and expa...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2013.0388
更新日期:2014-05-01 00:00:00
abstract::Human umbilical cord blood (CB) has been used successfully in stem cell transplantation. A subpopulation of CD34(+) cells expresses chemokine receptor CXCR4 which is critical for bone marrow engraftment in human hematopoietic stem cells. Here, we demonstrate the effect of short-term culture on CXCR4 expression on umbi...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2008.0298
更新日期:2009-10-01 00:00:00
abstract::Bone marrow-mesenchymal stem cells (BM-MSC) ameliorate renal dysfunction and repair tubular damage of acute kidney injury by locally releasing growth factors, including the insulin-like growth factor-1 (IGF-1). The restricted homing of BM-MSC at the site of injury led us to investigate a possible gene-based communicat...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2012.0266
更新日期:2013-03-01 00:00:00
abstract::CD41 (αIIb integrin), a specific marker for megakaryocytes and platelets, was recently shown to mark the initiation of definitive hematopoiesis in mouse embryos. However, whether embryonic CD41(+) populations have a nonhematopoietic potential remains elusive. Here, we report that the CD41(+) cells from the mouse E11.0...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2011.0572
更新日期:2012-09-20 00:00:00
abstract::Although the pluripotent and proliferative capacity of embryonic germ (EG) cells is thought to be equivalent to that of embryonic stem (ES) cells, there has been far less attention focused on the potential use of EG cells for applications in developing novel strategies of tissue transplantation in the treatment of deg...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2008.0383
更新日期:2010-02-01 00:00:00
abstract::The optimization of defined growth conditions is necessary for the development of clinical application of human embryonic stem cells (hESCs). Current research has focused on developing defined media formulations for long-term culture of hESCs with little attention on the establishment of defined substrates for hESC pr...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2008.0410
更新日期:2009-10-01 00:00:00
abstract::Adipose tissue (AT) is composed of mature adipocytes and stromal vascular fraction (SVF) cells, including adipose stem/stromal cells (ASCs). We characterized hematopoietic cells residing in human nonobese AT by analyzing the SVF isolated from human lipoaspirates and peripheral blood (PB). Flow cytometry revealed that ...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2012.0442
更新日期:2013-03-15 00:00:00
abstract::The secretome of human amniotic fluid stem cells (AFSCs) has great potential as a therapeutic agent in regenerative medicine. However, it must be produced in a clinically compliant manner before it can be used in humans. In this study, we developed a means of producing a biologically active secretome from AFSCs that i...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2017.0089
更新日期:2017-09-15 00:00:00
abstract::Special features of mesenchymal stem cells (MSCs) have made them a popular tool in cell therapy and tissue engineering. Although mouse animal models and murine MSCs are common tools in this field, our understanding of the effect of in vitro expansion on the behavior of these cells is poor and controversial. In additio...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2010.0266
更新日期:2011-08-01 00:00:00
abstract::Mechanical cues exert considerable influence on the fates of stem cells and terminally differentiated chondrocytes. The elucidation of the interactions between cell fate and mechanical cues in nuclear mechanotransduction will provide new clues to modulate tissue homeostasis and regeneration. In this study, we used an ...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2012.0685
更新日期:2013-07-15 00:00:00
abstract::Many of the currently established human embryonic stem (hES) cell lines have been characterized extensively in terms of their gene expression profiles and genetic stability in culture. Recent studies have indicated that microRNAs (miRNAs), a class of noncoding small RNAs that participate in the regulation of gene expr...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2007.0026
更新日期:2007-12-01 00:00:00
abstract::The regenerative capacity of the endometrium has been attributed to resident stem/progenitor cells. A number of stem/progenitor markers have been reported for human endometrial stem/progenitor cells; however, the lack of convenient markers in the mouse has made experimental investigation into endometrial regeneration ...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2018.0133
更新日期:2019-01-01 00:00:00