Abstract:
:Many of the currently established human embryonic stem (hES) cell lines have been characterized extensively in terms of their gene expression profiles and genetic stability in culture. Recent studies have indicated that microRNAs (miRNAs), a class of noncoding small RNAs that participate in the regulation of gene expression, may play a key role in stem cell self-renewal and differentiation. Using both microarrays and quantitative PCR, we report here the differences in miRNA expression between undifferentiated hES cells and their corresponding differentiated cells that underwent differentiation in vitro over a period of 2 weeks. Our results confirm the identity of a signature miRNA profile in pluripotent cells, comprising a small subset of differentially expressed miRNAs in hES cells. Examining both mRNA and miRNA profiles under multiple conditions using cross-correlation, we find clusters of miRNAs grouped with specific, biologically interpretable mRNAs. We identify patterns of expression in the progression from hES cells to differentiated cells that suggest a role for selected miRNAs in maintenance of the undifferentiated, pluripotent state. Profiling of the hES cell "miRNA-ome" provides an insight into molecules that control cellular differentiation and maintenance of the pluripotent state, findings that have broad implications in development, homeostasis, and human disease states.
journal_name
Stem Cells Devjournal_title
Stem cells and developmentauthors
Lakshmipathy U,Love B,Goff LA,Jörnsten R,Graichen R,Hart RP,Chesnut JDdoi
10.1089/scd.2007.0026subject
Has Abstractpub_date
2007-12-01 00:00:00pages
1003-16issue
6eissn
1547-3287issn
1557-8534journal_volume
16pub_type
杂志文章abstract::A long-term goal of mesenchymal progenitor cell (MPC) research is to identify cell-surface markers to facilitate MPC isolation. One reported MPC feature in humans and other species is lack of CD14 (lipopolysaccharide receptor) expression. The aim of this study was to evaluate CD14 as an MPC sorting marker. Our hypothe...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2010.0175
更新日期:2011-04-01 00:00:00
abstract::To better understand the basis of variation in cellular reprogramming, we performed experiments with two primary objectives: first, to determine the degree of difference, if any, in reprogramming efficiency among cells lines of a similar type after accounting for technical variables, and second, to compare the efficie...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2013.0040
更新日期:2013-10-01 00:00:00
abstract::Accumulating evidence suggests that the delivery of human adipose tissue-derived stromal cells (hASCs) has great potential as regenerative therapy. This was performed to develop a method for expanding hASCs by reducing the amount of serum required. We demonstrate that hASCs were able to expand efficiently in media con...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2008.0358
更新日期:2009-05-01 00:00:00
abstract::Progenitor expansion during development is a highly regulated process dictating the final organ size, while expansion of specific progenitor populators can adjust the final cellular composition of the organ. Understanding factors involved in these pathways is required to develop cell-based therapies such as β-cell tra...
journal_title:Stem cells and development
pub_type: 杂志文章,评审
doi:10.1089/scd.2013.0106
更新日期:2013-11-15 00:00:00
abstract::The major reservoir of human multipotent mesenchymal stem/stromal cells (MSCs) is the bone marrow (BM) with the capability to control hematopoietic stem cell development. The regenerative potential of MSCs is associated with enhanced endogenous repair and healing mechanisms that modulate inflammatory responses. Our pr...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2014.0509
更新日期:2015-08-01 00:00:00
abstract::Ex vivo cultures of human bone marrow-derived mesenchymal stem cells (MSCs) contain subsets of progenitors exhibiting dissimilar properties. One of these subsets comprises uncommitted progenitors displaying distinctive features, such as morphology, a quiescent condition, growth factor production, and restricted tissue...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2005.14.408
更新日期:2005-08-01 00:00:00
abstract::Müller glia are responsible for the retina regeneration observed in zebrafish. Although the human retina harbors Müller glia with stem cell characteristics, there is no evidence that they regenerate the retina after disease or injury. Transforming growth factor-β (TGFβ) and Wnt signaling regulate retinal neurogenesis ...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2015.0262
更新日期:2016-01-01 00:00:00
abstract::Bone marrow (BM)-derived mesenchymal stromal cells (MSCs) frequently display alterations in several hematologic disorders, such as acute lymphoid leukemia, acute myeloid leukemia (AML), and myelodysplastic syndromes. In acute leukemias, it is not clear whether MSC alterations contribute to the development of the malig...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2016.0295
更新日期:2017-05-15 00:00:00
abstract::Primary periodontal ligament stem cells (PDLSCs) are known to possess multidifferentiation potential and exhibit an immunophenotype similar to that described for bone-marrow-derived mesenchymal stem cells. In the present study, bromo-deoxyuridine (BrdU)-labeled ovine PDLSCs implanted into immunodeficient mice survived...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2013.0490
更新日期:2014-05-01 00:00:00
abstract::Tendons and ligaments (T/L) are dense connective tissues of mesodermal origin. During embryonic development, the tendon-specific cells descend from a sub-set of mesenchymal progenitors condensed in the syndetome, a dorsolateral domain of the sclerotome. These cells are defined by the expression of the transcription fa...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2011.0150
更新日期:2012-04-10 00:00:00
abstract::Multipotent stem/progenitor cells from bone marrow stroma (mesenchymal stromal cells or MSCs) were previously shown to enhance proliferation and differentiation of neural stem cells (NSCs) in vivo, but the molecular basis of the effect was not defined. Here coculturing human MSCs (hMSCs) with rat NSCs (rNSCs) was foun...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2009.0444
更新日期:2011-02-01 00:00:00
abstract::Multipotent mesenchymal stem/stromal cells (MSCs) are strongly involved in tissue homeostasis mainly through paracrine regulation. In this study, we examined the influence of simulated microgravity on the angiogenic potential of adipose-derived MSCs (ASCs). The conditioned medium (CM) from random positioning machine (...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2017.0262
更新日期:2018-06-15 00:00:00
abstract::The clinical use of stem cells in cell-based therapeutics for degenerative diseases requires development of criteria for defining normal stem cells to ensure safe transplantation. Currently, identification of abnormal from normal stem cells is based on extensive ex vivo and in vivo testing. Raman microscopy is a label...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2011.0600
更新日期:2012-08-10 00:00:00
abstract::The quality of hematopoietic stem cells (HSCs) is essentially defined by two characteristics, i.e., multilineage differentiation and self-renewal capacity. Thus, it is of high priority to clarify mechanisms that regulate these functions and to understand them at the molecular level. In the present study, we investigat...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2007.0107
更新日期:2008-04-01 00:00:00
abstract::Small-molecule inhibitors of glycogen synthase kinase 3β (GSK3β) have demonstrated strong anti-leukemia effects in preclinical studies. Here, we investigated the effect of GSK3β inhibitor 6-Bromoindirubin-3-oxime (BIO) previously shown to inhibit leukemia cell growth in vitro and of animal models on hematopoietic rege...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2014.0230
更新日期:2015-03-15 00:00:00
abstract::Pluripotent human embryonic stem cells (hESCs) provide appropriate systems for developmental studies and prospective donor cell sources for regenerative medicine. Identification of surface markers specific to hESCs is a prerequisite for studying hESC biology and can be used to generate clinical-level donor cell prepar...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2010.0265
更新日期:2011-04-01 00:00:00
abstract::The field of regenerative medicine has increasingly recognized the importance to be inspired by developmental processes to identify signaling pathways crucial for 3D organogenesis and tissue regeneration. Here, we aimed at recapitulating the first events occurring during limb development (ie, cell condensation and exp...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2013.0216
更新日期:2013-11-01 00:00:00
abstract::Multipotent bone marrow-derived mesenchymal stem/stromal cells (BMSCs) exhibit a finite life span after ex vivo expansion leading to cellular senescence. Many factors can contribute to this. Recently, our group has identified for the first time expression of the chemokine-like factor superfamily 8 (CMTM8) gene in cult...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2020.0007
更新日期:2020-07-01 00:00:00
abstract::Osteogenesis imperfecta (OI) is a genetic bone pathology with prenatal onset, characterized by brittle bones in response to abnormal collagen composition. There is presently no cure for OI. We previously showed that human first trimester fetal blood mesenchymal stem cells (MSCs) transplanted into a murine OI model (oi...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2013.0132
更新日期:2014-02-01 00:00:00
abstract::In recent years it has been hypothesized that cancer stem cells (CSCs) are the actual driving force of tumor formation, highlighting the need to specifically target CSCs to successfully eradicate cancer growth and recurrence. Particularly, the deregulation of physiological signaling pathways controlling stem cell prol...
journal_title:Stem cells and development
pub_type: 杂志文章,评审
doi:10.1089/scd.2015.0081
更新日期:2015-10-15 00:00:00
abstract::Fertility clinics worldwide routinely produce a large volume of 'waste' follicular aspirate, which is potentially an abundant source of immature ovarian follicles. Current attempts to cultivate these further in vitro to yield viable mature oocytes for fertility treatment have not yet achieved much success. Instead, re...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2005.14.11
更新日期:2005-02-01 00:00:00
abstract::The peritoneum mesothelium lines body cavities and has the same origin as ovarian surface epithelium with probable existence of peritoneum mesenchymal stem cells (PMSCs). In the present research, PMSCs were isolated from peritoneum and differentiated into ovarian cell-like cells using human follicular fluid (HFF) and ...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2018.0149
更新日期:2019-04-15 00:00:00
abstract::A common observation in metabolic disorders and aging is the elevation of free fatty acids, which can form ectopic fat deposition and result in lipotoxicity. Ectopic fat deposition of skeletal muscle has been recognized as an important component of aging, frailty and sarcopenia. Previous studies have suggested that li...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2020.0168
更新日期:2021-01-11 00:00:00
abstract::Stromal cells have been used to induce dopaminergic differentiation of mouse, primate, and human embryonic stem cells (hESCs), but the mechanism that governs this induction is unknown. In this manuscript, we show that medium conditioned by the stromal cell line PA6 (PA6-CM) can induce dopaminergic differentiation in n...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2009.0107
更新日期:2010-01-01 00:00:00
abstract::Human embryonic stem (hES) cells represent a potentially unlimited source of transplantable beta-cells for the treatment of diabetes. Here we describe a differentiation strategy that reproducibly directs HES3, an National Institutes of Health (NIH)-registered hES cell line, into cells of the pancreatic endocrine linea...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2007.0029
更新日期:2007-08-01 00:00:00
abstract::Ossification of the posterior longitudinal ligament (OPLL) is characterized by ectopic OPLL. To date, the specific molecular pathogenesis of OPLL has not been clearly elucidated. In this study, bone marrow-derived mesenchymal stem cells obtained from healthy donors (HD-MSCs) and patients with OPLL (OPLL-MSCs) were cul...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2020.0117
更新日期:2020-12-01 00:00:00
abstract::Mesenchymal stromal cells (MSCs) are multipotent stem cells with immunosuppressive and trophic support functions. While MSCs from different sources frequently display a similar appearance in culture, they often show differences in their surface marker and gene expression profiles. Although bone marrow is considered th...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2017.0029
更新日期:2018-01-15 00:00:00
abstract::Oligodendrogenesis encompasses lineage specification of neural progenitor cells (NPCs) and differentiation into oligodendrocytes that ultimately culminates in the myelination of central nervous system axons. Each individual process must be tightly regulated by extracellular and cell-intrinsic mechanisms, whose identit...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2011.0137
更新日期:2012-07-20 00:00:00
abstract::Smooth muscle cell (SMC) differentiation and dedifferentiation play a critical role in the pathogenesis of cardiovascular diseases. The lack of a good and simple in vitro SMC differentiation system has hampered the progress of SMC field for years. The generation of such an in vitro system would be invaluable for explo...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2008.0179
更新日期:2009-06-01 00:00:00
abstract::Umbilical cord blood (CB) CD34(+) cells, on the basis of flow cytometry analysis, are comprised of multiple populations. In in vitro assays, only CD34(regular) FSC(high) cells are functional and low percentages of nonfunctional CD34(regular) FSC(low) cells were determined to be present in liquid-stored CB. Liquid-stor...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2006.15.124
更新日期:2006-02-01 00:00:00