Abstract:
:Multipotent mesenchymal stem/stromal cells (MSCs) are strongly involved in tissue homeostasis mainly through paracrine regulation. In this study, we examined the influence of simulated microgravity on the angiogenic potential of adipose-derived MSCs (ASCs). The conditioned medium (CM) from random positioning machine (RPM)-exposed ASCs stimulated the formation of vessel network in ovo, endothelial cell (EC) capillary-like network, and nondirected EC migration in vitro. These effects were driven by alteration of both angiogenesis-related gene and protein expression. The elevation of angiogenic regulators Serpin E1, Serpin F1, IGFBP, VEGF, and IL-8 was detected in ASC-CM after 3D-clinorotation. In addition, transcription of genes encoding growth factors with proangiogenic activity were upregulated including VEGF-c and VEGF-a. These data evidenced that besides direct effect on ECs, microgravity could provoke MSC-mediating specific microenvironment for ECs supporting their functions, that is, proliferation and migration via increased production of IL-8 and VEGF as well as other paracrine factors involved in angiogenesis regulation.
journal_name
Stem Cells Devjournal_title
Stem cells and developmentauthors
Ratushnyy A,Ezdakova M,Yakubets D,Buravkova Ldoi
10.1089/scd.2017.0262subject
Has Abstractpub_date
2018-06-15 00:00:00pages
831-837issue
12eissn
1547-3287issn
1557-8534journal_volume
27pub_type
杂志文章abstract::Human umbilical cord blood (CB) has been used successfully in stem cell transplantation. A subpopulation of CD34(+) cells expresses chemokine receptor CXCR4 which is critical for bone marrow engraftment in human hematopoietic stem cells. Here, we demonstrate the effect of short-term culture on CXCR4 expression on umbi...
journal_title:Stem cells and development
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journal_title:Stem cells and development
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journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2011.0695
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journal_title:Stem cells and development
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journal_title:Stem cells and development
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journal_title:Stem cells and development
pub_type: 杂志文章
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journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2015.0345
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journal_title:Stem cells and development
pub_type: 杂志文章,评审
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journal_title:Stem cells and development
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journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2005.14.231
更新日期:2005-04-01 00:00:00
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journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2010.0494
更新日期:2011-11-01 00:00:00
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journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2012.0352
更新日期:2013-05-15 00:00:00
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journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2011.0150
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journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2018.0059
更新日期:2018-09-01 00:00:00
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journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2011.0243
更新日期:2012-05-20 00:00:00
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journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2017.0156
更新日期:2017-12-01 00:00:00
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journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2009.0093
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journal_title:Stem cells and development
pub_type: 杂志文章,评审
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journal_title:Stem cells and development
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journal_title:Stem cells and development
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journal_title:Stem cells and development
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journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2016.0054
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journal_title:Stem cells and development
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doi:10.1089/scd.2008.0383
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journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2014.0374
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journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2018.0239
更新日期:2019-07-15 00:00:00
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journal_title:Stem cells and development
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journal_title:Stem cells and development
pub_type: 杂志文章
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journal_title:Stem cells and development
pub_type: 杂志文章,评审
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更新日期:2008-06-01 00:00:00
abstract::Mouse Embryonic Stem Cells (mESCs) are unique in their self-renewal and pluripotency. Hypothetically, mESCs model gestational stress effects or stresses of in vitro fertilization/assisted reproductive technologies or drug/environmental exposures that endanger embryos. Testing mESCs stress responses should diminish and...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2018.0157
更新日期:2019-01-15 00:00:00