Abstract:
:The quality of hematopoietic stem cells (HSCs) is essentially defined by two characteristics, i.e., multilineage differentiation and self-renewal capacity. Thus, it is of high priority to clarify mechanisms that regulate these functions and to understand them at the molecular level. In the present study, we investigated the role of senescence evasion factor (synonymously hPrp19,hPSO4,hNMP200: SNEV), a multifunctional protein involved in pre-mRNA splicing, regulation of replicative life span, and DNA repair. Here we report that murine SNEV mRNA expression is high in lineage-depleted (Lin(-)) precursor cells of the bone marrow immediately after isolation as compared to fully differentiated peripheral blood lymphocytes (PBLs). Furthermore, the progenitor cell subset with highest colony-forming ability and self-renewal capacity (Lin(-), Sca-1(+)) showed also the highest SNEV expression. To test if the observed differences in SNEV mRNA levels cause stem cell defects, Lin(-) cells derived from heterozygous SNEV knockout mice were tested for primary as well as secondary colony-forming potential as a measure of self-renewal capacity. Interestingly, both, primary and secondary colonies were significantly less formed from SNEV(+/-) cells, a defect that was rescued by ectopic SNEV expression. Similarly, bone marrow cells derived from the short-lived Senescence-Accelerated-Mouse-Prone (SAMP8) model showed similar differences in comparison to the aging-resistant (SAMR1) control strain. These data suggest that the expression of SNEV is closely associated with the growth of murine HSCs and determines the proliferative and repopulating capacity of phenotypically defined HSC subsets.
journal_name
Stem Cells Devjournal_title
Stem cells and developmentauthors
Schraml E,Voglauer R,Fortschegger K,Sibilia M,Stelzer I,Grillari J,Schauenstein Kdoi
10.1089/scd.2007.0107subject
Has Abstractpub_date
2008-04-01 00:00:00pages
355-66issue
2eissn
1547-3287issn
1557-8534journal_volume
17pub_type
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pub_type: 杂志文章,评审
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journal_title:Stem cells and development
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journal_title:Stem cells and development
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journal_title:Stem cells and development
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journal_title:Stem cells and development
pub_type: 杂志文章
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journal_title:Stem cells and development
pub_type: 杂志文章
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journal_title:Stem cells and development
pub_type: 杂志文章,评审
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journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2012.0239
更新日期:2013-04-01 00:00:00
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journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2013.0396
更新日期:2014-04-01 00:00:00
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journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2013.0266
更新日期:2014-01-01 00:00:00
abstract::Human embryonic stem cells (hESCs) self-renew indefinitely as highly organized pluripotent colonies. Unlike mouse pluripotent stem cell colonies, human colonies form a uniform, flat, epithelium-like monolayer. Interestingly, it has been reported that colony morphology is closely correlated with the maintenance of plur...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2012.0288
更新日期:2013-01-15 00:00:00
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journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2007.0032
更新日期:2007-10-01 00:00:00
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journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2019.0145
更新日期:2020-06-01 00:00:00
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journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2007.0064
更新日期:2008-02-01 00:00:00
abstract::This study was undertaken to establish rat embryonic stem (ES) cells from parthenogenetically developing blastocysts. Ten blastocysts were prepared by treatment of ovulated rat oocytes with ionomycin and cycloheximide, and three alkaline phosphatase-positive ES cell lines were established using the N2B27 medium supple...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2013.0200
更新日期:2014-01-15 00:00:00
abstract::Bone marrow-derived mesenchymal stem cells (MSCs) possess multilineage differentiation potential and can be used for the treatment of diabetic heart failure. However, hyperglycemia can affect the function of MSCs adversely and merits the requirement for a strategy to correct this anomaly. MSCs were isolated from the t...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2009.0397
更新日期:2011-01-01 00:00:00
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journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2015.0339
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journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2009.0376
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journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2012.0067
更新日期:2012-09-20 00:00:00
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journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2017.0048
更新日期:2017-11-01 00:00:00
abstract::Red blood cell (RBC) differentiation from human induced pluripotent stem cells (hiPSCs) offers great potential for developmental studies and innovative therapies. However, ex vivo erythropoiesis from hiPSCs is currently limited by low efficiency and unphysiological conditions of common culture systems. Especially, the...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2019.0132
更新日期:2019-12-01 00:00:00
abstract::Accumulating evidence suggests that the delivery of human adipose tissue-derived stromal cells (hASCs) has great potential as regenerative therapy. This was performed to develop a method for expanding hASCs by reducing the amount of serum required. We demonstrate that hASCs were able to expand efficiently in media con...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2008.0358
更新日期:2009-05-01 00:00:00
abstract::The differentiation of human pluripotent stem cells to the B-cell lymphoid lineage has important clinical applications that include in vitro modeling of developmental lymphogenesis in health and disease. Here, we first demonstrate the capacity of human induced pluripotent stem cells (hiPSCs) to differentiate into CD14...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2014.0318
更新日期:2015-05-01 00:00:00
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journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2006.15.315
更新日期:2006-06-01 00:00:00
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journal_title:Stem cells and development
pub_type: 杂志文章
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journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2005.14.556
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