Abstract：
:Inducing a stable and predictable program of neural cell fate in pluripotent cells in vitro is an important goal for utilizing these cells for modeling human disease mechanisms. However, the extent to which in vitro neural specification recapitulates in vivo neural specification remains to be fully established. We previously demonstrated that in the mouse embryo, activation of fibroblast growth factor (FGF) signalling promotes definitive neural stem cell (NSC) development through the upregulation of the transcription factor Zfhx1b. Here, we asked whether Zfhx1b is similarly required during neural lineage development of embryonic stem (ES) cells. Zfhx1b gene expression is rapidly upregulated in mouse ES cells cultured in a permissive neural-inducing environment, compared to ES cells in a standard pluripotency maintenance environment, and is potentiated by FGF signalling. However, overexpression of Zfhx1b in ES cells in maintenance conditions, containing serum and leukemia inhibitory factor (LIF), is sufficient to induce Sox1 expression, a marker found in neural precursors and to promote definitive NSC colony formation. Knockdown of Zfhx1b in ES cells using siRNA did not affect the initial transition of ES cells to a neural cell fate, but did diminish the ability of these neural cells to develop further into definitive NSCs. Thus, our findings using ES cells are congruent with evidence from mouse embryos and support a model, whereby intercellular FGF signaling induces Zfhx1b, which promotes the development of definitive NSCs subsequent to an initial neural specification event that is independent of this pathway.
journal_name
Stem Cells Devjournal_title
Stem cells and developmentauthors
Dang LT,Wong L,Tropepe Vdoi
10.1089/scd.2011.0593subject
Has Abstractpub_date
2012-10-10 00:00:00pages
2838-51issue
15eissn
1547-3287issn
1557-8534journal_volume
21pub_type
杂志文章abstract：:Neuroblastoma (NB) is the second most common solid cancer in childhood, accounting for 15% of cancer-related deaths in children. In high-risk NB patients, the majority suffers from metastasis. Despite intensive multimodal treatment, long-term survival remains <40%. The bone marrow (BM) is among the most common sites o...
journal_title：Stem cells and development
pub_type： 杂志文章
doi：10.1089/scd.2020.0142
更新日期：2021-01-15 00:00:00
abstract：:Previously, we have shown that increasing the intracellular cGMP concentration enhances the sonic hedgehog (Shh) response in neural plate cells. The use of two mouse embryonic stem (ES) cell lines allowed a highly sensitive and reproducible quantification of the Shh response in neuralized embryoid bodies. Here we demo...
journal_title：Stem cells and development
pub_type： 杂志文章
doi：10.1089/scd.2006.15.647
更新日期：2006-10-01 00:00:00
abstract：:The capability to form all cell types of the body is a unique feature of stem cells. However, many questions remain concerning the mechanisms regulating differentiation potential. The derivation of spermatogonial cell lines (SGs) from mouse and human, which can differentiate across germ-layer borders, suggested male g...
journal_title：Stem cells and development
pub_type： 杂志文章
doi：10.1089/scd.2010.0290
更新日期：2011-08-01 00:00:00
abstract：:Human embryonic stem (hES) cells represent a potentially unlimited source of transplantable beta-cells for the treatment of diabetes. Here we describe a differentiation strategy that reproducibly directs HES3, an National Institutes of Health (NIH)-registered hES cell line, into cells of the pancreatic endocrine linea...
journal_title：Stem cells and development
pub_type： 杂志文章
doi：10.1089/scd.2007.0029
更新日期：2007-08-01 00:00:00
abstract：:Human stem cells from various sources have potential therapeutic applications. The clinical implementation of these therapies introduces the need for methods of noninvasive tracking of cells. The purpose of this study was to evaluate a high resolution magnetic resonance imaging (MRI) technique for in vivo detection an...
journal_title：Stem cells and development
pub_type： 杂志文章
doi：10.1089/scd.2008.0028
更新日期：2008-12-01 00:00:00
abstract：:Administration of human bone marrow-derived mesenchymal stem cells (BM-MSCs) significantly alleviates allergic airway inflammation. There are no studies that refer to the role of microRNAs (miRNAs) after the BM-MSCs treatment in airway allergic inflammation. We induced a mouse model of asthma and performed the transpl...
journal_title：Stem cells and development
pub_type： 杂志文章
doi：10.1089/scd.2015.0339
更新日期：2016-06-15 00:00:00
abstract：:Traditionally, undifferentiated pluripotent human embryonic and induced pluripotent stem cells (hESCs and hiPSCs) have been expanded as monolayer colonies in adhesion culture, both in the presence or absence of feeder cells. However, the use of pluripotent stem cells poses the need to scale-up current culture methods....
journal_title：Stem cells and development
pub_type： 杂志文章
doi：10.1089/scd.2010.0517
更新日期：2011-11-01 00:00:00
abstract：:Previous studies have transplanted a variety of neural stem cells (NSCs) to the eye in hopes of developing a therapy to replace retinal neurons lost to disease. Successful integration, survival, and differentiation of the cell types has been variably successful. At the moment, little is known about the fundamental bio...
journal_title：Stem cells and development
pub_type： 杂志文章
doi：10.1089/scd.2007.0051
更新日期：2008-02-01 00:00:00
abstract：:Regenerating islet (Reg) proteins are involved in the proliferation and differentiation of diverse cell types. However, whether embryonic stem cells (ESCs) express Reg genes and their corresponding proteins remains unknown. In this study, we probed the expression of Reg family members by mouse ESCs (mESCs). Mouse Reg1...
journal_title：Stem cells and development
pub_type： 杂志文章
doi：10.1089/scd.2009.0398
更新日期：2010-09-01 00:00:00
abstract：:Bone marrow (BM)-derived mesenchymal stromal cells (MSCs) frequently display alterations in several hematologic disorders, such as acute lymphoid leukemia, acute myeloid leukemia (AML), and myelodysplastic syndromes. In acute leukemias, it is not clear whether MSC alterations contribute to the development of the malig...
journal_title：Stem cells and development
pub_type： 杂志文章
doi：10.1089/scd.2016.0295
更新日期：2017-05-15 00:00:00
abstract：:How hematopoietic stem cells (HSCs) maintain the balance of self-renewal and differentiation could be partially ascribed to asymmetric and symmetric division patterns. However, a simple and effective method to detect stem cell division patterns is lacking. In this study, we introduce a strategy to describe stem cells ...
journal_title：Stem cells and development
pub_type： 杂志文章
doi：10.1089/scd.2018.0239
更新日期：2019-07-15 00:00:00
abstract：:Improvement in angiogenesis using mesenchymal stem cells (MSCs) is evolving as an option in patients with vascular insufficiencies. The paracrine factors secreted by MSCs have been attributed to the angiogenic response. This study was conducted to identify the factors secreted by umbilical cord-derived MSCs (UCMSCs) t...
journal_title：Stem cells and development
pub_type： 杂志文章
doi：10.1089/scd.2014.0184
更新日期：2015-02-15 00:00:00
abstract：:The development of methods to induce differentiation of human mesenchymal stem cells (hMSCs) has opened the possibility of using these cells in regenerative or reparative therapies. However, the low frequency of hMSCs in tissue means it is often necessary to expand these cells extensively in vitro. In this study, we e...
journal_title：Stem cells and development
pub_type： 杂志文章
doi：10.1089/scd.2007.0185
更新日期：2008-10-01 00:00:00
abstract：:Müller glia are responsible for the retina regeneration observed in zebrafish. Although the human retina harbors Müller glia with stem cell characteristics, there is no evidence that they regenerate the retina after disease or injury. Transforming growth factor-β (TGFβ) and Wnt signaling regulate retinal neurogenesis ...
journal_title：Stem cells and development
pub_type： 杂志文章
doi：10.1089/scd.2015.0262
更新日期：2016-01-01 00:00:00
abstract：:To understand which growth factors/cytokines can affect migration of mesenchymal stem cells (MSCs) to injured tissues, we compared the effects of many (26) growth factors/cytokines on the migration activity of rabbit and human MSCs using a microchemotaxis chamber. Among them, platelet-derived growth factor (PDGF)-BB, ...
journal_title：Stem cells and development
pub_type： 杂志文章
doi：10.1089/scd.2006.0032
更新日期：2007-02-01 00:00:00
abstract：:Pluripotent stem cells (PSCs), including embryonic stem cells (ESCs), and induced PSCs (iPSCs) are able to self-renew and differentiate into a multitude of specialized cellular lineages. In these cells, the pluripotential identity is maintained by a group of transcription factors (TFs). Among these factors, SOX TFs pl...
journal_title：Stem cells and development
pub_type： 杂志文章,评审
doi：10.1089/scd.2014.0297
更新日期：2014-11-15 00:00:00
abstract：:Traumatic spinal cord injury (SCI) leads to a cascade of secondary chemical insults, including oxidative stress and glutamate excitotoxicity, which damage host neurons and glia. Transplantation of exogenous neural stem/progenitor cells (NSPCs) has shown promise in enhancing regeneration after SCI, although survival of...
journal_title：Stem cells and development
pub_type： 杂志文章
doi：10.1089/scd.2015.0389
更新日期：2016-08-15 00:00:00
abstract：:Tendons and ligaments (T/L) are dense connective tissues of mesodermal origin. During embryonic development, the tendon-specific cells descend from a sub-set of mesenchymal progenitors condensed in the syndetome, a dorsolateral domain of the sclerotome. These cells are defined by the expression of the transcription fa...
journal_title：Stem cells and development
pub_type： 杂志文章
doi：10.1089/scd.2011.0150
更新日期：2012-04-10 00:00:00
abstract：:Stromal cells have been used to induce dopaminergic differentiation of mouse, primate, and human embryonic stem cells (hESCs), but the mechanism that governs this induction is unknown. In this manuscript, we show that medium conditioned by the stromal cell line PA6 (PA6-CM) can induce dopaminergic differentiation in n...
journal_title：Stem cells and development
pub_type： 杂志文章
doi：10.1089/scd.2009.0107
更新日期：2010-01-01 00:00:00
abstract：:Myocardial infarction (MI) results in loss of myofibers in the ischemic zone of the heart, followed by scar formation. These factors increase barriers to mobilization of mesenchymal stem cells (MSC), thereby impeding their effectiveness in cardiac repair. This study examined MSC overexpressing CXCR4 (MSC(CX4)) to dete...
journal_title：Stem cells and development
pub_type： 杂志文章
doi：10.1089/scd.2011.0126
更新日期：2012-03-20 00:00:00
abstract：:Multipotent mesenchymal stromal cells (MSCs) are more and more frequently used to treat orthopedic injuries in horses. However, these cells are limited in their expandability and differentiation capacity. Recently, the first equine-induced pluripotent stem cell (iPSC) lines were reported by us [ 1 ]. In vitro differen...
journal_title：Stem cells and development
pub_type： 杂志文章
doi：10.1089/scd.2014.0409
更新日期：2016-01-01 00:00:00
abstract：:In a previous study we established colony assays suitable for studying murine adult (2-4 months) pancreatic progenitor cells plated in semisolid media containing methylcellulose and extracellular matrix proteins. Using these assays, we found robust in vitro progenitor cell activities (multilineage differentiation and ...
journal_title：Stem cells and development
pub_type： 杂志文章
doi：10.1089/scd.2013.0466
更新日期：2014-04-15 00:00:00
abstract：:We have previously isolated mesenchymal stromal cells (MSCs) from the tracheal aspirates of premature neonates with respiratory distress. Although isolation of MSCs correlates with the development of bronchopulmonary dysplasia, the physiologic role of these cells remains unclear. To address this, we further characteri...
journal_title：Stem cells and development
pub_type： 杂志文章
doi：10.1089/scd.2010.0494
更新日期：2011-11-01 00:00:00
abstract：:Osteophytes are a distinct feature of osteoarthritis (OA). Their formation may be related to pluripotential cells in the periosteum responding to stimulus during OA. This study aimed to isolate stem cells from osteophyte tissues and to characterize their phenotype, proliferation, and differentiation potential, as well...
journal_title：Stem cells and development
pub_type： 杂志文章
doi：10.1089/scd.2007.0146
更新日期：2008-04-01 00:00:00
abstract：:Allergic asthma is an inflammatory airway disease caused by T helper type 2 (Th2)-driven immune responses. Recent studies have demonstrated that adipose-derived stromal cells (ASC) have an immunosuppressive effect on T-cell activity. This study was performed to investigate whether ASC can inhibit Th2-dependent allergi...
journal_title：Stem cells and development
pub_type： 杂志文章
doi：10.1089/scd.2009.0513
更新日期：2010-11-01 00:00:00
abstract：:Postnatal pancreas is a potential source for progenitor cells to generate endocrine β-cells for treating type 1 diabetes. However, it remains unclear whether young (1-week-old) pancreas harbors multipotent progenitors capable of differentiating into duct, acinar, and endocrine cells. Laminin is an extracellular matrix...
journal_title：Stem cells and development
pub_type： 杂志文章
doi：10.1089/scd.2015.0007
更新日期：2015-09-01 00:00:00
abstract：:Hematopoietic stem cells (HSCs) possess a distinct ability to perpetuate through self-renewal and to generate progeny that differentiate into mature cells of myeloid and lymphoid lineages. A better understanding of the molecular mechanisms by which HSCs replicate and differentiate from the perspective of developing ne...
journal_title：Stem cells and development
pub_type： 杂志文章,评审
doi：10.1089/scd.2006.15.755
更新日期：2006-12-01 00:00:00
abstract：:Regenerative medicine, a paragon of future healthcare, holds unprecedented potential in extending the reach of treatment modalities for individuals across diseases and lifespan. Emerging regenerative technologies, focused on structural repair and functional restoration, signal a radical transformation in medical and s...
journal_title：Stem cells and development
pub_type： 杂志文章
doi：10.1089/scd.2013.0448
更新日期：2013-12-01 00:00:00
abstract：:Hepatic ischemia reperfusion injury (IRI) remains a major obstacle in liver resection and transplantation surgery, especially in diseased organs. Human mesenchymal stromal cells (MSCs) are reported to acutely alleviate hepatic IRI in mice by releasing bioactive membrane-enclosed extracellular vesicles (EVs), but the l...
journal_title：Stem cells and development
pub_type： 杂志文章
doi：10.1089/scd.2019.0085
更新日期：2019-11-01 00:00:00
abstract：:A common observation in metabolic disorders and aging is the elevation of free fatty acids, which can form ectopic fat deposition and result in lipotoxicity. Ectopic fat deposition of skeletal muscle has been recognized as an important component of aging, frailty and sarcopenia. Previous studies have suggested that li...
journal_title：Stem cells and development
pub_type： 杂志文章
doi：10.1089/scd.2020.0168
更新日期：2021-01-11 00:00:00
