Abstract:
:To understand which growth factors/cytokines can affect migration of mesenchymal stem cells (MSCs) to injured tissues, we compared the effects of many (26) growth factors/cytokines on the migration activity of rabbit and human MSCs using a microchemotaxis chamber. Among them, platelet-derived growth factor (PDGF)-BB, PDGF-AB, epidermal growth factor (EGF), HB-EGF, transforming growth factor (TGF-alpha), insulin growth factor (IGF-I), hepatocyte growth factor (HGF), fibroblast growth factor (FGF-2), and thrombin consistently enhanced the migration of rabbit and human MSCs at appropriate concentrations. PDGF-BB showed the greatest effect on migration. Various combinations of these factors further enhanced the migration of MSCs, whereas combinations of factors that shared common cell-surface receptors did not induce the additive stimulation. On the other hand, some combinations, including that of FGF-2 or thrombin with PDGF-BB, suppressed the migration activity of MSCs. These findings suggest that combinations of growth factors are important to eliciting the maximal chemotactic effect. The factors that induced the migration of MSCs also enhanced their proliferation, suggesting that migration and proliferation can take place simultaneously. The above factors were also effective in stimulating the migration of fibroblasts, but thrombin alone selectively enhanced the migration of MSCs, suggesting that thrombin is useful to stimulate migration of MSCs without migration of fibroblasts.
journal_name
Stem Cells Devjournal_title
Stem cells and developmentauthors
Ozaki Y,Nishimura M,Sekiya K,Suehiro F,Kanawa M,Nikawa H,Hamada T,Kato Ydoi
10.1089/scd.2006.0032subject
Has Abstractpub_date
2007-02-01 00:00:00pages
119-29issue
1eissn
1547-3287issn
1557-8534journal_volume
16pub_type
杂志文章abstract::Mouse Embryonic Stem Cells (mESCs) are unique in their self-renewal and pluripotency. Hypothetically, mESCs model gestational stress effects or stresses of in vitro fertilization/assisted reproductive technologies or drug/environmental exposures that endanger embryos. Testing mESCs stress responses should diminish and...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2018.0157
更新日期:2019-01-15 00:00:00
abstract::Recent studies have revealed that mesenchymal stem cells (MSCs) have a great potential in therapeutic applications. The low efficiency of MSC recruitment and homing to sites of diseased organ tissue, however, remains a major hurdle in their application for treatment of diseases. Stress is commonly associated with vari...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2014.0243
更新日期:2015-03-01 00:00:00
abstract::Hematopoietic stem cells (HSCs) possess a distinct ability to perpetuate through self-renewal and to generate progeny that differentiate into mature cells of myeloid and lymphoid lineages. A better understanding of the molecular mechanisms by which HSCs replicate and differentiate from the perspective of developing ne...
journal_title:Stem cells and development
pub_type: 杂志文章,评审
doi:10.1089/scd.2006.15.755
更新日期:2006-12-01 00:00:00
abstract::Mechanical cues exert considerable influence on the fates of stem cells and terminally differentiated chondrocytes. The elucidation of the interactions between cell fate and mechanical cues in nuclear mechanotransduction will provide new clues to modulate tissue homeostasis and regeneration. In this study, we used an ...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2012.0685
更新日期:2013-07-15 00:00:00
abstract::Mesenchymal stem cells (MSCs) from bone marrow (BM) are widely used for bone and less for cartilage tissue regeneration due to their self-renewal and differentiating properties into osteogenic or chondrogenic lineages. This review considers the last decade of clinical trials involving a two-step procedure, by expandin...
journal_title:Stem cells and development
pub_type: 杂志文章,评审
doi:10.1089/scd.2012.0373
更新日期:2013-01-15 00:00:00
abstract::Human perivascular progenitor cells, including pericytes, are well-described multipotent mesenchymal cells giving rise to mesenchymal stem cells in culture. Despite the unique location of pericytes, specific antigens to distinguish human pericytes from other cell types are few. Here, we employed a human tissue microar...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2019.0106
更新日期:2019-09-15 00:00:00
abstract::Acute respiratory distress syndrome (ARDS)/acute lung injury (ALI) is a severe clinical respiratory failure disorder associated with chronic pathology and disability and has a mortality rate of 40%-60%. However, the pathogenesis of ARDS/ALI remains unclear, and existing therapeutic options are insufficient for address...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2020.0174
更新日期:2021-02-01 00:00:00
abstract::We recently reported the isolation and characterization of a population of pancreatic progenitor cells (PPCs) from early trimester human fetal pancreata. The PPCs, being the forerunners of adult pancreatic cell lineages, were amenable to growth and differentiation into insulin-secreting islet-like cell clusters (ICCs)...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2008.0325
更新日期:2009-09-01 00:00:00
abstract::Induced pluripotent stem cells (iPSCs) are somatic cells that have been reprogrammed to a pluripotent state via introduction of defined transcription factors. iPSCs are a valuable resource for regenerative medicine, but whether iPSCs are identical to embryonic stem cells (ESCs) remains unclear. In this study, we perfo...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2011.0243
更新日期:2012-05-20 00:00:00
abstract::Chronic idiopathic neutropenia (CIN) is a granulopoiesis disorder associated with an inhibitory bone marrow (BM) microenvironment consisting of activated T-lymphocytes and pro-inflammatory mediators. In this study, we investigated the possible involvement of BM mesenchymal stem cells (MSCs) in the pathophysiology of C...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2010.0425
更新日期:2011-08-01 00:00:00
abstract::The design of reliable biocompatible and biodegradable scaffolds remains one of the most important challenges for tissue engineering. In fact, properly designed scaffolds must display an adequate and interconnected porosity to facilitate cell spreading and colonization of the inner layers, and must release physical si...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2017.0051
更新日期:2017-10-01 00:00:00
abstract::Human embryonic stem cells (hESCs) have the potential to differentiate into many adult cell types, and they are being explored as a resource for cell replacement therapies for multiple diseases. In order to optimize in vitro differentiation protocols, it will be necessary to elucidate regulatory mechanisms that contri...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2009.0224
更新日期:2010-06-01 00:00:00
abstract::Human stem cells from various sources have potential therapeutic applications. The clinical implementation of these therapies introduces the need for methods of noninvasive tracking of cells. The purpose of this study was to evaluate a high resolution magnetic resonance imaging (MRI) technique for in vivo detection an...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2008.0028
更新日期:2008-12-01 00:00:00
abstract::Rare heterozygous deletions in the neurexin 1 (NRXN1) gene robustly increase an individual's risk of developing neurological and psychiatric disorders. However, the molecular bases by which different mutations result in different clinical presentations, with variable penetrance, are unknown. To better understand the m...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2020.0017
更新日期:2020-09-01 00:00:00
abstract::Myocardial infarction (MI) results in loss of myofibers in the ischemic zone of the heart, followed by scar formation. These factors increase barriers to mobilization of mesenchymal stem cells (MSC), thereby impeding their effectiveness in cardiac repair. This study examined MSC overexpressing CXCR4 (MSC(CX4)) to dete...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2011.0126
更新日期:2012-03-20 00:00:00
abstract::Allogeneic stem cell (SC)-based therapy is a promising tool for the treatment of a range of human degenerative and inflammatory diseases. Many reports highlighted the immune modulatory properties of some SC types, such as mesenchymal stromal cells (MSCs), but a comparative study with SCs of different origin, to assess...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2013.0204
更新日期:2013-11-15 00:00:00
abstract::Although umbilical cord blood (UCB) has been widely accepted as an alternative source of hematopoietic stem cells (HSC) for transplantation, its use in adults is restricted because of low absolute HSC numbers. To overcome this obstacle, expansion of HSC in coculture with feeder cells is a promising possibility. In thi...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2007.0273
更新日期:2009-01-01 00:00:00
abstract::Cell therapy with adult mesenchymal stem cells (MSCs) is a promising approach to regenerative medicine and autoimmune diseases. There are various approaches to improve the efficacy of MSC-based therapeutics, and MSC preparation as spheroidal aggregates, or MSC spheroids, is a novel preparatory and delivery method. Sph...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2015.0356
更新日期:2016-04-15 00:00:00
abstract::A murine stromal cell line (OP9-DL1) expressing a notch ligand, Delta-like-1, has been shown to be able to drive the differentiation of both murine and human hematopoietic progenitors into T cells in vitro. Further studies showed that hematopoietic progenitors transduced by a retroviral vector to express a human CD8 T...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2008.0021
更新日期:2009-03-01 00:00:00
abstract::Individuals with neurodegenerative disorders or brain injury have few treatment options and it has been proposed that endogenous adult neural stem cells can be harnessed to repopulate dysfunctional nonneurogenic regions of the brain. We have accomplished this through the development of rationally designed hydrogel imp...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2016.0069
更新日期:2016-08-01 00:00:00
abstract::Stem cell-based therapies have demonstrated great potential for the treatment of cardiac diseases, for example, myocardial infarction; however, low cell viability, low retention/engraftment, and uncontrollable in vivo differentiation after transplantation are still major limitations, which lead to low therapeutic effi...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2016.0351
更新日期:2017-06-15 00:00:00
abstract::The differentiation of human pluripotent stem cells to the B-cell lymphoid lineage has important clinical applications that include in vitro modeling of developmental lymphogenesis in health and disease. Here, we first demonstrate the capacity of human induced pluripotent stem cells (hiPSCs) to differentiate into CD14...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2014.0318
更新日期:2015-05-01 00:00:00
abstract::Collectively, lung diseases are one of the largest causes of premature death worldwide and represent a major focus in the field of regenerative medicine. Despite significant progress, only few stem cell platforms are currently available for cell-based therapy, disease modeling, and drug screening in the context of pul...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2014.0512
更新日期:2015-07-15 00:00:00
abstract::Cyclooxygenase-2 (COX-2) inhibitors suppress bone repair and bone formation by suppressing angiogenesis as well as potentially interfering with osteoblast and osteoclast functions. In spite of these reports, there is a controversy over the exact effects of COX-2 inhibitors on bone formation processes itself. This work...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2009.0393
更新日期:2010-10-01 00:00:00
abstract::Studies on rodent bone marrow stromal cells (MSCs) have revealed a capacity, for at least a portion of cells, to express neuron-like traits after differentiation in culture. Little, however, is known about the ability of human MSCs in this regard. We show here that incubation with certain differentiation cocktails, pa...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2004.13.625
更新日期:2004-12-01 00:00:00
abstract::Misexpression of stem cell-related genes may occur in some cancer cells, influencing patient's prognosis. This is the case of medulloblastoma, a common and clinically challenging malignant tumor of the central nervous system, where expression of the pluripotency factor, OCT4, is correlated with poor survival. A downst...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2015.0052
更新日期:2015-11-15 00:00:00
abstract::Regenerating islet (Reg) proteins are involved in the proliferation and differentiation of diverse cell types. However, whether embryonic stem cells (ESCs) express Reg genes and their corresponding proteins remains unknown. In this study, we probed the expression of Reg family members by mouse ESCs (mESCs). Mouse Reg1...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2009.0398
更新日期:2010-09-01 00:00:00
abstract::A common observation in metabolic disorders and aging is the elevation of free fatty acids, which can form ectopic fat deposition and result in lipotoxicity. Ectopic fat deposition of skeletal muscle has been recognized as an important component of aging, frailty and sarcopenia. Previous studies have suggested that li...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2020.0168
更新日期:2021-01-11 00:00:00
abstract::The mammalian inner ear is vulnerable to genetic disorders and aging, as well as to injuries caused by overstimulation, ototoxic drugs, and viral infections. Due to the poor regeneration of the sensory epithelium and the spiral ganglion neurons in the adult mammalian inner ear, cell replacement therapy strategies have...
journal_title:Stem cells and development
pub_type: 杂志文章,评审
doi:10.1089/scd.2006.15.449
更新日期:2006-06-01 00:00:00
abstract::Most disease-modifying therapeutic approaches in Alzheimer's disease (AD) aim to reduce the accumulation of neurotoxic amyloid-beta (Abeta) peptides as a hallmark of AD pathogenesis. Here we report the in vitro basis for a potential autologous stem cell-based strategy for widespread delivery of enzymatic activities ag...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2009.0045
更新日期:2010-05-01 00:00:00