Abstract:
:A common observation in metabolic disorders and aging is the elevation of free fatty acids, which can form ectopic fat deposition and result in lipotoxicity. Ectopic fat deposition of skeletal muscle has been recognized as an important component of aging, frailty and sarcopenia. Previous studies have suggested that lipotoxicity caused by free fatty acids mainly stemmed from saturated fatty acids and decreased unsaturated/ saturated fatty acid ratio in serum are also observed among metabolic disorder patients. However, the different effects of saturated fatty acids and unsaturated fatty acids on skeletal muscle are not fully differentiative capacity of C2C12 myoblasts by affecting Pax7, MyoD and MyoG, which are master regulators of lineage specification and the myogenic program. Then, oleate (OA), a monounsaturated fatty acid, were added to culture medium together with PA. Results showed that OA could ameliorate the impairment of differentiative capacity in C2C12 myoblast cells. In addition, we found PI3K/Akt signaling pathway played an important role during the process by RNA-sequencing and bioinformatics analysis. The positive effect of OA on myoblast differentiative capacity disappeared if PI3K inhibitor LY294002 was added. In conclusion, our study showed that PA could destroy differentiative capacity of C2C12 myoblasts by affecting the expression of Pax7, MyoD and MyoG, and OA could improve this impairment through PI3K /Akt signaling pathway.
journal_name
Stem Cells Devjournal_title
Stem cells and developmentauthors
Xue M,Zhang F,Ji X,Yu H,Jiang X,Qiu Y,Yu J,Chen J,Yang F,Bao Zdoi
10.1089/scd.2020.0168subject
Has Abstractpub_date
2021-01-11 00:00:00eissn
1547-3287issn
1557-8534pub_type
杂志文章abstract::Acute respiratory distress syndrome (ARDS)/acute lung injury (ALI) is a severe clinical respiratory failure disorder associated with chronic pathology and disability and has a mortality rate of 40%-60%. However, the pathogenesis of ARDS/ALI remains unclear, and existing therapeutic options are insufficient for address...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2020.0174
更新日期:2021-02-01 00:00:00
abstract::Diseases of the digestive tract are complex and encompass a broad spectrum of different pathogeneses (inflammatory, ischemic, neoplastic, and functional deficit). The digestive tract is not a sterile environment, and its organs are composed of tissues with different embryologic origin and different morphologic and fun...
journal_title:Stem cells and development
pub_type: 杂志文章,评审
doi:10.1089/scd.2010.0277
更新日期:2011-07-01 00:00:00
abstract::The development of the heart is essential for embryogenesis and precedes development of other organs. However, the mechanisms involved in embryonic cardiac development are ill-defined. Recent evidence suggests that Smad and Wnt signaling pathways are important in stem cell fate determination and their commitment to ca...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2006.15.29
更新日期:2006-02-01 00:00:00
abstract::Epoxyeicosatrienoic acid (EET) contributes to browning of white adipose stem cells to ameliorate obesity/diabetes and insulin resistance. In the current study, we show that EET altered preadipocyte function, enhanced peroxisome proliferation-activated receptor γ coactivator α (PGC-1α) expression, and increased mitocho...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2016.0072
更新日期:2016-07-15 00:00:00
abstract::We have isolated a novel progenitor cell population from adult rat pancreatic ducts, termed pancreatic-derived progenitor cells (PDPCs). Here, we report the in vitro culture, selection, and characterization of Thy1.1-positive and Thy1.1-negative PDPC subpopulations. These cells exhibit bipotentiality for differentiati...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2008.0301
更新日期:2009-12-01 00:00:00
abstract::Small molecules (SM) can greatly enhance the efficiency of induced pluripotent stem (iPS) cell generation, but the mechanisms by which they act have not been fully explored. We show here that an SM cocktail (NaB, PD03259, and SB431542) significantly promotes iPS cell generation from human fibroblasts, and NaB is more ...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2012.0650
更新日期:2013-08-15 00:00:00
abstract::Improvement in angiogenesis using mesenchymal stem cells (MSCs) is evolving as an option in patients with vascular insufficiencies. The paracrine factors secreted by MSCs have been attributed to the angiogenic response. This study was conducted to identify the factors secreted by umbilical cord-derived MSCs (UCMSCs) t...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2014.0184
更新日期:2015-02-15 00:00:00
abstract::The generation of Schwann cells from precursors within adult skin and bone marrow is of significant clinical interest because of the opportunities for disease modelling and strategies for remyelination. Recent evidence has suggested that glial cells can be generated from (i) mesenchymal stem cells (MSCs) within adult ...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2009.0371
更新日期:2010-07-01 00:00:00
abstract::Humans and nonhuman primates (NHPs) are similar in size, behavior, physiology, biochemistry, structure and function of organs, and complexity of the immune system. Research on NHPs generates complementary data that bridge translational research from small animal models to humans. NHP models of human disease offer uniq...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2014.0374
更新日期:2014-12-01 00:00:00
abstract::Resistance to imatinib can occur in patients with chronic myelogenous leukemia (CML). In this study, we report mechanisms of action of histone deacetylase (HDAC) inhibitor, depsipeptide (FK228) in BCR/ABL-expressing cell lines and its effectiveness in imatinib-resistant cells from patients with blast crisis of CML. FK...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2007.9994
更新日期:2007-06-01 00:00:00
abstract::Multipotent skin-derived progenitors (SKPs) can be traced back to embryonic neural crest cells and are able to differentiate into both neural and mesodermal progeny in vitro. Neural stem cells (NSCs) are capable of self-renewing and can contribute to neuron and glia in the nervous system. Recently, we derived porcine ...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2012.0067
更新日期:2012-09-20 00:00:00
abstract::The capability to form all cell types of the body is a unique feature of stem cells. However, many questions remain concerning the mechanisms regulating differentiation potential. The derivation of spermatogonial cell lines (SGs) from mouse and human, which can differentiate across germ-layer borders, suggested male g...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2010.0290
更新日期:2011-08-01 00:00:00
abstract::Embryonic stem-like (ES-like) cells have now been derived from the inner cell mass (ICM) of horse embryos at the blastocyst stage. Because they have been shown to express cell-surface antigens found in both human and mouse ES cells, the present study investigated gene expression patterns in day-7 horse blastocysts fro...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2007.0032
更新日期:2007-10-01 00:00:00
abstract::After intra-articular injection, synovium-derived mesenchymal stem cells (SMSCs) can adhere to damaged cartilage (a process called homing) and then repair the cartilage defect. Nonetheless, the main obstacle of the current method is the insufficient homing ratio of SMSCs, which fails to meet the requirements for carti...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2019.0137
更新日期:2020-04-01 00:00:00
abstract::Vascular smooth muscle cells (SMCs), which arise from multiple embryonic progenitors, have unique lineage-specific properties and this diversity may contribute to spatial patterns of vascular diseases. We developed in vitro methods to generate distinct vascular SMC subtypes from human pluripotent stem cells, allowing ...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2014.0520
更新日期:2015-04-01 00:00:00
abstract::In this study, we isolated and characterized spontaneously differentiated human embryonic stem cells (SD-hESCs) found in hESC colonies in comparison to the morphologically premature ESCs in the colonies to investigate the potential role of SD-hESCs in embryogenesis. SD-hESCs were distinguished from undifferentiated hE...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2013.0083
更新日期:2013-10-15 00:00:00
abstract::The aryl hydrocarbon receptor (AhR) belongs to the basic helix-loop-helix family of DNA-binding proteins that play a role in the toxicity and carcinogenicity of certain chemicals. The most potent ligand of the AhR known is 2,3,7,8-tetracholorodibenzo-p-dioxin. We previously reported tetrachlorodibenzo-p-dioxin-induced...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2010.0333
更新日期:2011-05-01 00:00:00
abstract::Human umbilical cord blood (CB) has been used successfully in stem cell transplantation. A subpopulation of CD34(+) cells expresses chemokine receptor CXCR4 which is critical for bone marrow engraftment in human hematopoietic stem cells. Here, we demonstrate the effect of short-term culture on CXCR4 expression on umbi...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2008.0298
更新日期:2009-10-01 00:00:00
abstract::Bone marrow (BM)-derived mesenchymal stromal cells (MSCs) frequently display alterations in several hematologic disorders, such as acute lymphoid leukemia, acute myeloid leukemia (AML), and myelodysplastic syndromes. In acute leukemias, it is not clear whether MSC alterations contribute to the development of the malig...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2016.0295
更新日期:2017-05-15 00:00:00
abstract::Cyclooxygenase-2 (COX-2) inhibitors suppress bone repair and bone formation by suppressing angiogenesis as well as potentially interfering with osteoblast and osteoclast functions. In spite of these reports, there is a controversy over the exact effects of COX-2 inhibitors on bone formation processes itself. This work...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2009.0393
更新日期:2010-10-01 00:00:00
abstract::Studies on rodent bone marrow stromal cells (MSCs) have revealed a capacity, for at least a portion of cells, to express neuron-like traits after differentiation in culture. Little, however, is known about the ability of human MSCs in this regard. We show here that incubation with certain differentiation cocktails, pa...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2004.13.625
更新日期:2004-12-01 00:00:00
abstract::Bone marrow-mesenchymal stem cells (BM-MSC) ameliorate renal dysfunction and repair tubular damage of acute kidney injury by locally releasing growth factors, including the insulin-like growth factor-1 (IGF-1). The restricted homing of BM-MSC at the site of injury led us to investigate a possible gene-based communicat...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2012.0266
更新日期:2013-03-01 00:00:00
abstract::Aneuploidy is commonly seen in human preimplantation embryos, most particularly at the cleavage stage because of genome activation by third cell division. Aneuploid embryos have been used for the derivation of normal embryonic stem cell (ESC) lines and developmental modeling. This review addresses aneuploidies in huma...
journal_title:Stem cells and development
pub_type: 杂志文章,评审
doi:10.1089/scd.2013.0053
更新日期:2013-09-01 00:00:00
abstract::Mesenchymal stem cells (MSCs) have attracted much attention as potential platforms for transgene delivery and cell-based therapy for human disease. MSCs have the capability to self-renew and retain multipotency after extensive expansion in vitro, making them attractive targets for ex vivo modification and autologous t...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2007.0194
更新日期:2008-06-01 00:00:00
abstract::Small-molecule inhibitors of glycogen synthase kinase 3β (GSK3β) have demonstrated strong anti-leukemia effects in preclinical studies. Here, we investigated the effect of GSK3β inhibitor 6-Bromoindirubin-3-oxime (BIO) previously shown to inhibit leukemia cell growth in vitro and of animal models on hematopoietic rege...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2014.0230
更新日期:2015-03-15 00:00:00
abstract::Multipotent mesenchymal stem/stromal cells (MSCs) are strongly involved in tissue homeostasis mainly through paracrine regulation. In this study, we examined the influence of simulated microgravity on the angiogenic potential of adipose-derived MSCs (ASCs). The conditioned medium (CM) from random positioning machine (...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2017.0262
更新日期:2018-06-15 00:00:00
abstract::Ex vivo cultures of human bone marrow-derived mesenchymal stem cells (MSCs) contain subsets of progenitors exhibiting dissimilar properties. One of these subsets comprises uncommitted progenitors displaying distinctive features, such as morphology, a quiescent condition, growth factor production, and restricted tissue...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2005.14.408
更新日期:2005-08-01 00:00:00
abstract::Regenerative medicine, a paragon of future healthcare, holds unprecedented potential in extending the reach of treatment modalities for individuals across diseases and lifespan. Emerging regenerative technologies, focused on structural repair and functional restoration, signal a radical transformation in medical and s...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2013.0448
更新日期:2013-12-01 00:00:00
abstract::Human perivascular progenitor cells, including pericytes, are well-described multipotent mesenchymal cells giving rise to mesenchymal stem cells in culture. Despite the unique location of pericytes, specific antigens to distinguish human pericytes from other cell types are few. Here, we employed a human tissue microar...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2019.0106
更新日期:2019-09-15 00:00:00
abstract::Stem cell therapy is a promising therapeutic strategy for treating myocardial infarction (MI). However, it is necessary to identify ideal adult stem cells for transplantation and explore mechanisms of the transplanted cells in improving cardiac functions after MI. In this study, a population of embryonic-like stem cel...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2011.0280
更新日期:2012-05-20 00:00:00