Abstract:
:Recent studies have revealed that mesenchymal stem cells (MSCs) have a great potential in therapeutic applications. The low efficiency of MSC recruitment and homing to sites of diseased organ tissue, however, remains a major hurdle in their application for treatment of diseases. Stress is commonly associated with various diseases. At the present time, little information is available about the effect of stress on MSC function. Here, we employed a carbon tetrachloride (CCl4)-induced mouse liver fibrosis model to investigate whether constraint stress affects the migration of MSCs to fibrotic liver. MSC homing to the fibrotic liver was significantly inhibited in mice with restraint stress. Restraint stress induced an elevation of corticosterone level in the serum. Blocking glucocorticoid signaling with either corticosterone-synthesis inhibitor metyrapone (MET) or glucocorticoid receptor antagonist RU486 attenuated restraint stress-induced inhibition of MSCs migration. The serum concentration of stromal cell-derived factor-1 (SDF-1) increased in mice treated with CCl4. Restraint stress had no influence on expression of SDF-1 and hepatocyte growth factor (HGF) in the fibrotic liver. Culture with the serum of CCl4-treated mice or SDF-1 promoted MSC migration, which was suppressed by corticosterone. Exposure of MSCs to corticosterone decreased their expression of C-X-C chemokine receptor type 4 (CXCR4) and C-X-C chemokine receptor type 7 (CXCR7). These results demonstrate that the inhibitory effect of corticosterone on MSC migration might be mediated via decreasing the expression of CXCR4 and CXCR7 in MSCs. Interventions targeting the interaction between corticosterone and its receptor improve migration and homing of MSCs in hosts receiving transplantation of these cells.
journal_name
Stem Cells Devjournal_title
Stem cells and developmentauthors
Zhang S,Lv C,Yang X,Han Z,Zhang S,Zhang J,Zong C,Gao L,Li L,Zhao Q,Li R,Yang Y,Yu F,Li X,Zhang P,Wei Ldoi
10.1089/scd.2014.0243subject
Has Abstractpub_date
2015-03-01 00:00:00pages
587-96issue
5eissn
1547-3287issn
1557-8534journal_volume
24pub_type
杂志文章abstract::The quality of hematopoietic stem cells (HSCs) is essentially defined by two characteristics, i.e., multilineage differentiation and self-renewal capacity. Thus, it is of high priority to clarify mechanisms that regulate these functions and to understand them at the molecular level. In the present study, we investigat...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2007.0107
更新日期:2008-04-01 00:00:00
abstract::Mesenchymal stem cells (MSCs) have a superior immunomodulatory capacity compared to other cells of the immune system, and they hold great promise for treating various immune disorders. However, their regulatory effects on the maturation of immature dendritic cells (imDCs) are not fully understood. In this study, we sh...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2017.0235
更新日期:2018-04-15 00:00:00
abstract::Ex vivo cultures of human bone marrow-derived mesenchymal stem cells (MSCs) contain subsets of progenitors exhibiting dissimilar properties. One of these subsets comprises uncommitted progenitors displaying distinctive features, such as morphology, a quiescent condition, growth factor production, and restricted tissue...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2005.14.408
更新日期:2005-08-01 00:00:00
abstract::Cell-based gene therapy holds a great promise for the treatment of human malignancy. Among different cells, mesenchymal stem cells (MSCs) are emerging as valuable anti-cancer agents that have the potential to be used to treat a number of different cancer types. They have inherent migratory properties, which allow them...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2016.0120
更新日期:2016-10-01 00:00:00
abstract::Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) has been used extensively in cancer therapy. However, more than half of glioblastoma multiforme are insensitive to the apoptotic effect of TRAIL. Improvement in therapeutic modalities that enhances the efficacy of TRAIL in glioma is much sought after. In ...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2012.0529
更新日期:2013-07-01 00:00:00
abstract::Reduction of estradiol production and high serum concentrations of follicular stimulating hormone (FSH) are endocrine disorders associated with premature ovarian failure. Here, we report that transplantation of ovarian-like cells differentiated from stem cells restored endogenous serum estradiol levels. Stem cells wer...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2014.0029
更新日期:2014-07-15 00:00:00
abstract::Electrical stimulation (ES) of cells has been shown to induce a variety of responses, such as cytoskeleton rearrangements, migration, proliferation, and differentiation. In this study, we have investigated whether monophasic and biphasic pulsed ES could exert any effect on the proliferation and differentiation of huma...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2013.0375
更新日期:2014-04-15 00:00:00
abstract::Neuroblastoma (NB) is the second most common solid cancer in childhood, accounting for 15% of cancer-related deaths in children. In high-risk NB patients, the majority suffers from metastasis. Despite intensive multimodal treatment, long-term survival remains <40%. The bone marrow (BM) is among the most common sites o...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2020.0142
更新日期:2021-01-15 00:00:00
abstract::A common observation in metabolic disorders and aging is the elevation of free fatty acids, which can form ectopic fat deposition and result in lipotoxicity. Ectopic fat deposition of skeletal muscle has been recognized as an important component of aging, frailty and sarcopenia. Previous studies have suggested that li...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2020.0168
更新日期:2021-01-11 00:00:00
abstract::Erythropoietin (Epo) may be considered as an endogenous stimulator of vessel growth during tumor progression through an autocrine and/or paracrine loop. The vascular effects of Epo would be relevant in tumor angiogenesis and the negative effect of Epo on tumor growth may be aggravated by its angiogenic activity. The m...
journal_title:Stem cells and development
pub_type: 杂志文章,评审
doi:10.1089/scd.2009.0402
更新日期:2010-01-01 00:00:00
abstract::Human embryonic stem cells (hESCs) exhibiting skewed X chromosome inactivation (XCI) have been reported. The copy number variations (CNVs), loss of heterozygosity (LOH), or single-nucleotide variant (SNV) events in those epigenetically distinct cells remain unknown, and whether such genetic abnormalities will influenc...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2014.0463
更新日期:2015-08-01 00:00:00
abstract::N-methyl-d-aspartate receptors (NMDARs) are expressed abundantly in the brain and play an important role in the regulation of neuronal development, learning, memory, neurodegenerative diseases, and neurogenesis, all of which are extensively discussed in reviews published elsewhere. While the number of research article...
journal_title:Stem cells and development
pub_type: 杂志文章,评审
doi:10.1089/scd.2016.0325
更新日期:2017-06-01 00:00:00
abstract::Stromal cells have been used to induce dopaminergic differentiation of mouse, primate, and human embryonic stem cells (hESCs), but the mechanism that governs this induction is unknown. In this manuscript, we show that medium conditioned by the stromal cell line PA6 (PA6-CM) can induce dopaminergic differentiation in n...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2009.0107
更新日期:2010-01-01 00:00:00
abstract::Although umbilical cord blood (UCB) has been widely accepted as an alternative source of hematopoietic stem cells (HSC) for transplantation, its use in adults is restricted because of low absolute HSC numbers. To overcome this obstacle, expansion of HSC in coculture with feeder cells is a promising possibility. In thi...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2007.0273
更新日期:2009-01-01 00:00:00
abstract::Postnatal pancreas is a potential source for progenitor cells to generate endocrine β-cells for treating type 1 diabetes. However, it remains unclear whether young (1-week-old) pancreas harbors multipotent progenitors capable of differentiating into duct, acinar, and endocrine cells. Laminin is an extracellular matrix...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2015.0007
更新日期:2015-09-01 00:00:00
abstract::Multipotent mesenchymal stromal cells (MSCs) are more and more frequently used to treat orthopedic injuries in horses. However, these cells are limited in their expandability and differentiation capacity. Recently, the first equine-induced pluripotent stem cell (iPSC) lines were reported by us [ 1 ]. In vitro differen...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2014.0409
更新日期:2016-01-01 00:00:00
abstract::The increasing use of mesenchymal stromal cells (MSC) in clinical cellular therapy requires a safe and controlled production process compliant with Good Manufacturing Practice guidelines. Pooled blood group AB human serum (HS) has been used to replace fetal bovine serum (FBS), critically rated by the regulatory agenci...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2012.0051
更新日期:2013-03-01 00:00:00
abstract::While transplantation of human induced pluripotent stem cell-derived neural stem cells (hiPSC-NSCs) shows therapeutic potential in animal stroke models, major concerns for translating hiPSC therapy to the clinic are efficacy and safety. Therefore, there is a demand to develop an optimal strategy to enhance the engraft...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2018.0055
更新日期:2018-08-15 00:00:00
abstract::Conditioned media (CM) of transformed cells, such as the human lung-derived A549 cells, is a useful tool for directing differentiation of embryonic stem cells (ESCs). Previous work indicates that A549-CM induced pulmonary differentiation of mouse ESCs (mESCs). In this study, we compared the effects of A549-CM treatmen...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2014.0042
更新日期:2014-08-15 00:00:00
abstract::Cell replacement therapy holds great promise for treating a wide range of human disorders. However, ensuring the predictable differentiation of transplanted stem cells, eliminating their risk of tumor formation, and generating fully functional cells after transplantation remain major challenges in regenerative medicin...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2010.0555
更新日期:2011-11-01 00:00:00
abstract::Induced pluripotent stem cell (iPSC) reprogramming requires sustained expression of multiple reprogramming factors for a limited period of time (10-30 days). Conventional iPSC reprogramming was achieved using lentiviral or simple retroviral vectors. Retroviral reprogramming has flaws of insertional mutagenesis, uncont...
journal_title:Stem cells and development
pub_type: 杂志文章,评审
doi:10.1089/scd.2013.0621
更新日期:2014-06-15 00:00:00
abstract::Embryonic stem cells (ESCs) have the capacity to form all the tissues in the body and hence, directed differentiation of ESCs along specific lineages represents a means to generate therapeutically useful cell types. It has been postulated that, during in vitro differentiation, ES cells sequentially pass through simila...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2005.14.656
更新日期:2005-12-01 00:00:00
abstract::Adipose tissue (AT) is composed of mature adipocytes and stromal vascular fraction (SVF) cells, including adipose stem/stromal cells (ASCs). We characterized hematopoietic cells residing in human nonobese AT by analyzing the SVF isolated from human lipoaspirates and peripheral blood (PB). Flow cytometry revealed that ...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2012.0442
更新日期:2013-03-15 00:00:00
abstract::Red blood cell (RBC) differentiation from human induced pluripotent stem cells (hiPSCs) offers great potential for developmental studies and innovative therapies. However, ex vivo erythropoiesis from hiPSCs is currently limited by low efficiency and unphysiological conditions of common culture systems. Especially, the...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2019.0132
更新日期:2019-12-01 00:00:00
abstract::To better understand the basis of variation in cellular reprogramming, we performed experiments with two primary objectives: first, to determine the degree of difference, if any, in reprogramming efficiency among cells lines of a similar type after accounting for technical variables, and second, to compare the efficie...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2013.0040
更新日期:2013-10-01 00:00:00
abstract::The generation of Schwann cells from precursors within adult skin and bone marrow is of significant clinical interest because of the opportunities for disease modelling and strategies for remyelination. Recent evidence has suggested that glial cells can be generated from (i) mesenchymal stem cells (MSCs) within adult ...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2009.0371
更新日期:2010-07-01 00:00:00
abstract::Myocardial infarction is a major public health problem that causes significant mortality despite recent advances in its prevention and treatment. Therefore, approaches based on adult stem cells represent a promising alternative to conventional therapies for this life-threatening condition. Mesenchymal stem cells (MSCs...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2008.0292
更新日期:2009-07-01 00:00:00
abstract::The projected annual demand for stem cell-based dental treatments in the United States can range from over 290 million tooth restorative treatments to 30,000 patients requiring tissue regeneration following craniofacial cancer surgery. Professional dental treatments cost Americans over $60 billion per year. Scientific...
journal_title:Stem cells and development
pub_type: 杂志文章,评审
doi:10.1089/scd.2006.15.881
更新日期:2006-12-01 00:00:00
abstract::The CD34 surface antigen has been recognized as a marker of hematopoietic stem cells (HSCs) and is widely used for HSC selection as well as for quality control in HSC transplantation. CD34 has been implicated in cytoadhesion signaling, and its expression has been suggested to reflect the activation state of hematopoie...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2006.0056
更新日期:2007-04-01 00:00:00
abstract::Over the past 20 months, reports claiming the generation of induced pluripotent stem (iPS) cells with characteristics identical to those of embryonic stem (ES) cells from nonembryonic tissue have captured great attention in both the scientific community and the general public. In the light of the continuing controvers...
journal_title:Stem cells and development
pub_type: 杂志文章,评审
doi:10.1089/scd.2008.0062
更新日期:2008-06-01 00:00:00