Abstract:
:Regenerating islet (Reg) proteins are involved in the proliferation and differentiation of diverse cell types. However, whether embryonic stem cells (ESCs) express Reg genes and their corresponding proteins remains unknown. In this study, we probed the expression of Reg family members by mouse ESCs (mESCs). Mouse Reg1 and Reg3gamma were detected in undifferentiated stem cells. Furthermore, we tested if gastrin--an inducer of Reg1 expression in committed cells--up-regulates the Reg1 gene in mESCs. Gastrin did not affect the expression of Reg1 either in self-renewing mESCs or under conditions permitting their differentiation. Moreover, overexpression of Reg genes found in various forms of cancer has been linked to dysregulated activation of the canonical Wnt/beta-catenin cascade. Given the important roles of Wnt signaling in stem cells, we investigated if activation of Wnt alters the expression of Reg genes in mESCs. Wnt activation led to an increase in Reg1 gene expression with a concomitant increase in the amount of secreted Reg1 protein. Finally, the expression pattern of genes indicative of differentiation was examined in mESCs that were either exposed to soluble Reg1 or overexpressed the Reg1 gene. This is the first account of expression of Reg family members by ESCs. Our results show that the canonical Wnt cascade affects Reg expression and warrants further studies into the potential roles of Reg proteins in stem cell physiology.
journal_name
Stem Cells Devjournal_title
Stem cells and developmentauthors
Jing D,Kehoe DE,Tzanakakis ESdoi
10.1089/scd.2009.0398subject
Has Abstractpub_date
2010-09-01 00:00:00pages
1307-19issue
9eissn
1547-3287issn
1557-8534journal_volume
19pub_type
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journal_title:Stem cells and development
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journal_title:Stem cells and development
pub_type: 杂志文章,评审
doi:10.1089/scd.2016.0325
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journal_title:Stem cells and development
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journal_title:Stem cells and development
pub_type: 杂志文章
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journal_title:Stem cells and development
pub_type: 杂志文章,评审
doi:10.1089/scd.2013.0621
更新日期:2014-06-15 00:00:00
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journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2011.0280
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journal_title:Stem cells and development
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doi:10.1089/scd.2018.0239
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journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2020.0168
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journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2015.0317
更新日期:2016-03-01 00:00:00
abstract::The regeneration of lost periodontal ligament (PDL) and alveolar bone is the purpose of periodontal tissue engineering. The goal of the present study was to assess the suitability of 3 odontogenic progenitor populations from dental pulp, PDL, and dental follicle for periodontal regeneration when exposed to natural and...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2010.0431
更新日期:2011-10-01 00:00:00
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journal_title:Stem cells and development
pub_type: 杂志文章
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journal_title:Stem cells and development
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更新日期:2014-10-01 00:00:00
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journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2014.0029
更新日期:2014-07-15 00:00:00
abstract::Adipose-derived stem cells (ADSCs) are a type of multipotent mesenchymal stem cells with immunosuppressive capacities. However, the underlying mechanisms involved in the inhibitory effects of ADSCs on T cells are not completely elucidated. In this study, human peripheral blood mononuclear cells (PBMCs) stimulated with...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2018.0033
更新日期:2018-09-01 00:00:00
abstract::In the past few years research on stem cells has exploded as a tool to develop potential therapies to treat incurable neurodegenerative diseases. Stem cell transplantation has been effective in several animal models, but the underlying restorative mechanisms are still unknown. Several events such as cell fusion, neuro...
journal_title:Stem cells and development
pub_type: 杂志文章,评审
doi:10.1089/154732804773099326
更新日期:2004-02-01 00:00:00
abstract::Novel cell-based and gene therapies represent promising approaches for the treatment of incurable diseases, including cancer. Following the success of the hematopoietic stem cell-based transplantation, other populations of adult progenitor cells, including mesenchymal stem cells (MSCs), have been identified as powerfu...
journal_title:Stem cells and development
pub_type: 杂志文章,评审
doi:10.1089/scd.2010.0223
更新日期:2011-01-01 00:00:00
abstract::The development of methods to induce differentiation of human mesenchymal stem cells (hMSCs) has opened the possibility of using these cells in regenerative or reparative therapies. However, the low frequency of hMSCs in tissue means it is often necessary to expand these cells extensively in vitro. In this study, we e...
journal_title:Stem cells and development
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更新日期:2008-10-01 00:00:00
abstract::As granulocyte-colony-stimulating factor (G-CSF)-induced mobilization of hematopoietic stem/progenitor cells (HSPCs) increases human serum levels of hepatocyte growth factor (HGF), our aim was to investigate the role of HGF and its receptor, c-Met, in the mobilization of HSPC. CD34(+) cells and leukocytes were isolate...
journal_title:Stem cells and development
pub_type: 杂志文章
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更新日期:2010-08-01 00:00:00
abstract::Multipotent stem/progenitor cells from bone marrow stroma (mesenchymal stromal cells or MSCs) were previously shown to enhance proliferation and differentiation of neural stem cells (NSCs) in vivo, but the molecular basis of the effect was not defined. Here coculturing human MSCs (hMSCs) with rat NSCs (rNSCs) was foun...
journal_title:Stem cells and development
pub_type: 杂志文章
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更新日期:2011-02-01 00:00:00