Epigenetic analysis and suitability of amniotic fluid stem cells for research and therapeutic purposes.

abstract：:To understand which growth factors/cytokines can affect migration of mesenchymal stem cells (MSCs) to injured tissues, we compared the effects of many (26) growth factors/cytokines on the migration activity of rabbit and human MSCs using a microchemotaxis chamber. Among them, platelet-derived growth factor (PDGF)-BB, ...

journal_title：Stem cells and development

authors： Ozaki Y,Nishimura M,Sekiya K,Suehiro F,Kanawa M,Nikawa H,Hamada T,Kato Y

更新日期：2007-02-01 00:00:00

abstract：:The AMP-activated protein kinase (AMPK) mediates rapid, stress-induced loss of the inhibitor of differentiation (Id)2 in blastocysts and trophoblast stem cells (TSC), and a lasting differentiation in TSC. However, it is not known if AMPK regulates other potency factors or regulates them before the blastocyst stage. Th...

journal_title：Stem cells and development

authors： Xie Y,Awonuga A,Liu J,Rings E,Puscheck EE,Rappolee DA

更新日期：2013-05-15 00:00:00

abstract：:A murine stromal cell line (OP9-DL1) expressing a notch ligand, Delta-like-1, has been shown to be able to drive the differentiation of both murine and human hematopoietic progenitors into T cells in vitro. Further studies showed that hematopoietic progenitors transduced by a retroviral vector to express a human CD8 T...

journal_title：Stem cells and development

authors： Dai B,Wang P

更新日期：2009-03-01 00:00:00

abstract：:The CD34 surface antigen has been recognized as a marker of hematopoietic stem cells (HSCs) and is widely used for HSC selection as well as for quality control in HSC transplantation. CD34 has been implicated in cytoadhesion signaling, and its expression has been suggested to reflect the activation state of hematopoie...

journal_title：Stem cells and development

authors： Lange C,Li Z,Fang L,Baum C,Fehse B

更新日期：2007-04-01 00:00:00

abstract：:In this retrospective study, 209 patients with malignant disease were analyzed for levels of T-cell receptor excision circles (TRECs) for the first 24 months after allogeneic stem cell transplantation. CD3(+) cells were separated by direct antibody-coupled magnetic beads, followed by DNA extraction according to a stan...

journal_title：Stem cells and development

authors： Uzunel M,Sairafi D,Remberger M,Mattsson J,Uhlin M

更新日期：2014-07-15 00:00:00

abstract：:Special features of mesenchymal stem cells (MSCs) have made them a popular tool in cell therapy and tissue engineering. Although mouse animal models and murine MSCs are common tools in this field, our understanding of the effect of in vitro expansion on the behavior of these cells is poor and controversial. In additio...

journal_title：Stem cells and development

authors： Ahmadbeigi N,Soleimani M,Gheisari Y,Vasei M,Amanpour S,Bagherizadeh I,Shariati SA,Azadmanesh K,Amini S,Shafiee A,Arabkari V,Nardi NB

更新日期：2011-08-01 00:00:00

abstract：:Regenerative therapy of the salivary gland (SG) is a promising therapeutic approach for irreversible hyposalivation in patients with head and neck cancer treated by radiotherapy. However, little is known about the molecular regulators of stem/progenitor cell activity and regenerative processes in the SG. Wnt/β-catenin...

journal_title：Stem cells and development

authors： Hai B,Yang Z,Millar SE,Choi YS,Taketo MM,Nagy A,Liu F

更新日期：2010-11-01 00:00:00

abstract：:In this study we examine whether a somatic cell, once returned to a pluripotent state, gains the ability to reprogram other somatic cells. We reprogrammed mouse embryonic fibroblasts by viral induction of oct4, sox2, c-myc, and klf-4 genes. Upon fusion of the resulting iPS cells with somatic cells harboring an Oct4-GF...

journal_title：Stem cells and development

authors： Sumer H,Jones KL,Liu J,Heffernan C,Tat PA,Upton KR,Verma PJ

更新日期：2010-02-01 00:00:00

abstract：:We describe a method of generating an enriched population of NCAM-positive cells from a human teratocarcinoma cell line (NTera2/D1) and their differentiation into midbrain dopaminergic neurons in the absence of the caudalizing factor retinoic acid (RA). NTera2 cells were induced to form embryoid bodies and then to gen...

journal_title：Stem cells and development

authors： Ravindran G,Rao HS

更新日期：2006-08-01 00:00:00

abstract：:The aim of this study was to clarify the mechanisms that regulate hematopoietic cell expansion in vitro by identifying defined culture conditions. We report the results of experiments with CD34(+) cells from cord blood (CB, n = 13), bone marrow (BM, n = 4), and mobilized peripheral blood stem cells (PBSC, n = 5) using...

journal_title：Stem cells and development

authors： Rossi B,Zanolin E,Vincenzi C,Diani F,Pizzolo G,de Wynter E,Nadali G

更新日期：2004-08-01 00:00:00

abstract：:The chemokine stromal cell-derived factor (SDF)-1α/CXCL12 and its receptor CXC chemokine receptor 4 (CXCR4) play a crucial role in the homing/engraftment and retention of hematopoietic stem/progenitor cells (HSPCs) in the bone marrow. It has been shown using the viral gene transfer technique that CXCR4 overexpression ...

journal_title：Stem cells and development

authors： Gul-Uludag H,Xu P,Marquez-Curtis LA,Xing J,Janowska-Wieczorek A,Chen J

更新日期：2012-07-01 00:00:00

abstract：:Diseases of the digestive tract are complex and encompass a broad spectrum of different pathogeneses (inflammatory, ischemic, neoplastic, and functional deficit). The digestive tract is not a sterile environment, and its organs are composed of tissues with different embryologic origin and different morphologic and fun...

journal_title：Stem cells and development

authors： Trebol Lopez J,Georgiev Hristov T,García-Arranz M,García-Olmo D

更新日期：2011-07-01 00:00:00

abstract：:Mouse Embryonic Stem Cells (mESCs) are unique in their self-renewal and pluripotency. Hypothetically, mESCs model gestational stress effects or stresses of in vitro fertilization/assisted reproductive technologies or drug/environmental exposures that endanger embryos. Testing mESCs stress responses should diminish and...

journal_title：Stem cells and development

authors： Li Q,Louden E,Zhou J,Drewlo S,Dai J,Puscheck EE,Chen K,Rappolee DA

更新日期：2019-01-15 00:00:00

abstract：:Recent studies have focused on transcriptional regulation and gene expression profiling of human embryonic stem cells (hESCs). However, little information is available regarding the relationship between RNA expression and transcriptional regulation, which is critical in the complete understanding of pluripotency and d...

journal_title：Stem cells and development

authors： Player A,Wang Y,Bhattacharya B,Rao M,Puri RK,Kawasaki ES

更新日期：2006-06-01 00:00:00

abstract：:Here, we describe a focused microarray for screening rat embryonic stem cells (ESCs) and provide validation data that this array can distinguish undifferentiated rat ESCs from rat trophoblast stem (TS) cells, rat extraembryonic endoderm cells, mouse embryonic fibroblast feeder cells, and differentiated rat ESCs. Using...

journal_title：Stem cells and development

authors： Hong J,He H,Bui P,Ryba-White B,Rumi MA,Soares MJ,Dutta D,Paul S,Kawamata M,Ochiya T,Ying QL,Rajanahalli P,Weiss ML

更新日期：2013-02-01 00:00:00

abstract：:The aim of stem cell therapy after cardiac injury is to replace damaged cardiac tissue. Human cardiac progenitor cells (CPCs) represent an interesting cell population for clinical strategies to treat cardiac disease and human CPC-specific antibodies would aid in the clinical implementation of cardiac progenitor-based ...

journal_title：Stem cells and development

authors： Moerkamp AT,Leung HW,Bax NAM,Holst S,Lodder K,Berends T,Dingenouts CKE,Choo A,Smits AM,Goumans MJ

更新日期：2017-11-01 00:00:00

abstract：:In this work, we evaluated the expansion of human hematopoietic stem cells from umbilical cord blood in roller bottles. The Iscove's modified Dulbecco's medium, the Stem Pro 34-SFM medium, and the L-15 Leibovitz's medium for cultures in CO(2)-free atmosphere were assessed. At day 5 of culture, total colony forming uni...

journal_title：Stem cells and development

authors： Andrade-Zaldívar H,Kalixto-Sánchez MA,de la Rosa AP,De León-Rodríguez A

更新日期：2011-04-01 00:00:00

abstract：:Intermediate progenitor populations play a crucial role in the regional specification and differentiation of the cranial neural crest. On the basis of global gene expression profiles, gene cohort expression levels, and epigenetic modifications, we have defined key factors involved in the differentiation of dental foll...

journal_title：Stem cells and development

authors： Dangaria SJ,Ito Y,Luan X,Diekwisch TG

更新日期：2011-01-01 00:00:00

abstract：:Subarachnoid hemorrhage (SAH) is a type of hemorrhagic stroke with significant morbidity and mortality, and it often leads to poor clinical outcome. Although great efforts have been made toward animal and clinical studies, optimal therapy of SAH remains a challenge for scientists and clinicians. Increasing evidence su...

journal_title：Stem cells and development

authors： Song Z,Zhang JH

更新日期：2020-02-15 00:00:00

abstract：:Phenotypic guidance of embryonic stem (ES) cell fate is paramount if these cells are to be used for tissue repair and regeneration. Our objective was to compare two different cell culture feeders and their effect on proliferation, apoptosis, and differentiation of human (h) ES cells. HSF-6 hES cells were grown in Knoc...

journal_title：Stem cells and development

authors： Ozolek JA,Jane EP,Krowsoski L,Sammak PJ

更新日期：2007-06-01 00:00:00

abstract：:The enhanced proliferation of mesenchymal stem cells (MSCs) can be helpful for the clinical translation of cell therapy. Low-level laser irradiation (LLLI) has been demonstrated as regulating MSC proliferation. MicroRNAs (miRNAs) are involved in various pathophysiologic processes in stem cells, but the role of miRNAs ...

journal_title：Stem cells and development

authors： Wang J,Huang W,Wu Y,Hou J,Nie Y,Gu H,Li J,Hu S,Zhang H

更新日期：2012-09-01 00:00:00

abstract：:To better understand the basis of variation in cellular reprogramming, we performed experiments with two primary objectives: first, to determine the degree of difference, if any, in reprogramming efficiency among cells lines of a similar type after accounting for technical variables, and second, to compare the efficie...

journal_title：Stem cells and development

authors： Alicea B,Murthy S,Keaton SA,Cobbett P,Cibelli JB,Suhr ST

更新日期：2013-10-01 00:00:00

abstract：:Geminin is a multifunctional protein previously suggested to both maintain the bone morphogenetic protein inhibition required for neural induction and to control cell-cycle progression and cell fate in the early embryo. Since Geminin is required in the blastocyst on E3.5, we employed shRNA to examine its role during p...

journal_title：Stem cells and development

authors： Emmett LS,O'Shea KS

更新日期：2012-09-01 00:00:00

abstract：:In spina bifida, the neural tube fails to close during the embryonic period. Exposure of the neural tube to the amniotic fluid during pregnancy causes additional neural damage. Intrauterine tissue engineering using a biomaterial seeded with stem cells might prevent this additional damage. For this purpose, autologous ...

journal_title：Stem cells and development

authors： Hosper NA,Bank RA,van den Berg PP

更新日期：2014-03-01 00:00:00

abstract：:The generation of Schwann cells from precursors within adult skin and bone marrow is of significant clinical interest because of the opportunities for disease modelling and strategies for remyelination. Recent evidence has suggested that glial cells can be generated from (i) mesenchymal stem cells (MSCs) within adult ...

journal_title：Stem cells and development

authors： Hunt DP,Sajic M,Phillips H,Henderson D,Compston A,Smith K,Chandran S

更新日期：2010-07-01 00:00:00

abstract：:Neural stem cells (NSCs) have become promising tools for basic research and regenerative medicine. Intracerebral transplantation studies have suggested that these cells may be able to adopt neuronal phenotypes typical of their engraftment site and to establish appropriate connections in the recipient circuitries. Here...

journal_title：Stem cells and development

authors： Brilli E,Reitano E,Conti L,Conforti P,Gulino R,Consalez GG,Cesana E,Smith A,Rossi F,Cattaneo E

更新日期：2013-02-15 00:00:00

abstract：:Death ligands and their tumor necrosis factor receptor (TNFR) family receptors are the best-characterized and most efficient inducers of apoptotic signaling in somatic cells. In this study, we analyzed whether these prototypic activators of apoptosis are also expressed and able to be activated in human pluripotent ste...

journal_title：Stem cells and development

authors： Vinarsky V,Krivanek J,Rankel L,Nahacka Z,Barta T,Jaros J,Andera L,Hampl A

更新日期：2013-11-15 00:00:00

abstract：:Mesenchymal stromal cells (MSCs) hold great promise in the field of regenerative medicine due to their ability to create a variable localized anti-inflammatory effect in injuries such as Crohn's disease and osteoarthritis or by incorporation in tissue engineered constructs. Currently, the MSC literature uses rodents f...

journal_title：Stem cells and development

authors： Wright A,Snyder L,Knights K,He H,Springer NL,Lillich J,Weiss ML

更新日期：2020-06-01 00:00:00

abstract：:Postnatal pancreas is a potential source for progenitor cells to generate endocrine β-cells for treating type 1 diabetes. However, it remains unclear whether young (1-week-old) pancreas harbors multipotent progenitors capable of differentiating into duct, acinar, and endocrine cells. Laminin is an extracellular matrix...

journal_title：Stem cells and development

authors： Ghazalli N,Mahdavi A,Feng T,Jin L,Kozlowski MT,Hsu J,Riggs AD,Tirrell DA,Ku HT

更新日期：2015-09-01 00:00:00

abstract：:Multipotent skin-derived progenitors (SKPs) can be traced back to embryonic neural crest cells and are able to differentiate into both neural and mesodermal progeny in vitro. Neural stem cells (NSCs) are capable of self-renewing and can contribute to neuron and glia in the nervous system. Recently, we derived porcine ...

journal_title：Stem cells and development

authors： Zhao MT,Yang X,Lee K,Mao J,Teson JM,Whitworth KM,Samuel MS,Spate LD,Murphy CN,Prather RS

更新日期：2012-09-20 00:00:00