Abstract：
:Mesenchymal stromal cells (MSCs) hold great promise in the field of regenerative medicine due to their ability to create a variable localized anti-inflammatory effect in injuries such as Crohn's disease and osteoarthritis or by incorporation in tissue engineered constructs. Currently, the MSC literature uses rodents for preclinical disease models. There is growing interest in using naturally occurring disease in large animals for modeling human disease. By review of the canine MSCs literature, it appears that canine MSCs can be difficult to maintain in culture for extended passages and this greatly varies between tissue sources, compared with human and rodent MSCs, and limited lifespan is an obstacle for preclinical investigation and therapeutic use. Research using canine MSCs has been focused on cells derived from bone marrow or adipose tissue, and the differences in manufacturing MSCs between laboratories are problematic due to lack of standardization. To address these issues, here, a stepwise process was used to optimize canine MSCs isolation, expansion, and cryopreservation utilizing canine umbilical cord-derived MSCs. The culture protocol utilizes coating of tissue culture surfaces that increases cellular adherence, increases colony-forming units-fibroblast efficiency, and decreases population doubling times. Canine MSCs isolated with our protocol could be maintained longer than published canine MSCs methods before senescing. Our improved cryopreservation protocols produce on average >90% viable MSCs at thaw. These methods enable master-bank and working-bank scenarios for allogeneic MSC testing in naturally occurring disease in dogs.
journal_name
Stem Cells Devjournal_title
Stem cells and developmentauthors
Wright A,Snyder L,Knights K,He H,Springer NL,Lillich J,Weiss MLdoi
10.1089/scd.2019.0145subject
Has Abstractpub_date
2020-06-01 00:00:00pages
695-713issue
11eissn
1547-3287issn
1557-8534journal_volume
29pub_type
杂志文章abstract：:The capability to form all cell types of the body is a unique feature of stem cells. However, many questions remain concerning the mechanisms regulating differentiation potential. The derivation of spermatogonial cell lines (SGs) from mouse and human, which can differentiate across germ-layer borders, suggested male g...
journal_title：Stem cells and development
pub_type： 杂志文章
doi：10.1089/scd.2010.0290
更新日期：2011-08-01 00:00:00
abstract：:Herein we review recent data that support host tolerance of allogeneic adult mesenchymal stem cells (MSC). Evidence is emerging that donor MSC deploy a very powerful array of mechanisms that allow escape from host allogeneic responses. These mechanisms include limited expression of alloantigen by the stem cell and cel...
journal_title：Stem cells and development
pub_type： 杂志文章,评审
doi：10.1089/scd.2005.14.252
更新日期：2005-06-01 00:00:00
abstract：:Primordial germ cells (PGCs), the precursors of gametes, have regulatory mechanisms involving transcription factors and epigenetic modifications. The transcription factor NANOG is a key regulator of germ cell and embryonic stem cell characteristics. However, the epigenetic regulation of NANOG with a histone deacetylas...
journal_title：Stem cells and development
pub_type： 杂志文章
doi：10.1089/scd.2018.0059
更新日期：2018-09-01 00:00:00
abstract：:Vascular smooth muscle cells (SMCs), which arise from multiple embryonic progenitors, have unique lineage-specific properties and this diversity may contribute to spatial patterns of vascular diseases. We developed in vitro methods to generate distinct vascular SMC subtypes from human pluripotent stem cells, allowing ...
journal_title：Stem cells and development
pub_type： 杂志文章
doi：10.1089/scd.2014.0520
更新日期：2015-04-01 00:00:00
abstract：:The major reservoir of human multipotent mesenchymal stem/stromal cells (MSCs) is the bone marrow (BM) with the capability to control hematopoietic stem cell development. The regenerative potential of MSCs is associated with enhanced endogenous repair and healing mechanisms that modulate inflammatory responses. Our pr...
journal_title：Stem cells and development
pub_type： 杂志文章
doi：10.1089/scd.2014.0509
更新日期：2015-08-01 00:00:00
abstract：:Over the past 20 months, reports claiming the generation of induced pluripotent stem (iPS) cells with characteristics identical to those of embryonic stem (ES) cells from nonembryonic tissue have captured great attention in both the scientific community and the general public. In the light of the continuing controvers...
journal_title：Stem cells and development
pub_type： 杂志文章,评审
doi：10.1089/scd.2008.0062
更新日期：2008-06-01 00:00:00
abstract：:Cell therapy with adult mesenchymal stem cells (MSCs) is a promising approach to regenerative medicine and autoimmune diseases. There are various approaches to improve the efficacy of MSC-based therapeutics, and MSC preparation as spheroidal aggregates, or MSC spheroids, is a novel preparatory and delivery method. Sph...
journal_title：Stem cells and development
pub_type： 杂志文章
doi：10.1089/scd.2015.0356
更新日期：2016-04-15 00:00:00
abstract：:The aim of stem cell therapy after cardiac injury is to replace damaged cardiac tissue. Human cardiac progenitor cells (CPCs) represent an interesting cell population for clinical strategies to treat cardiac disease and human CPC-specific antibodies would aid in the clinical implementation of cardiac progenitor-based ...
journal_title：Stem cells and development
pub_type： 杂志文章
doi：10.1089/scd.2017.0048
更新日期：2017-11-01 00:00:00
abstract：:Adipose tissue (AT) is composed of mature adipocytes and stromal vascular fraction (SVF) cells, including adipose stem/stromal cells (ASCs). We characterized hematopoietic cells residing in human nonobese AT by analyzing the SVF isolated from human lipoaspirates and peripheral blood (PB). Flow cytometry revealed that ...
journal_title：Stem cells and development
pub_type： 杂志文章
doi：10.1089/scd.2012.0442
更新日期：2013-03-15 00:00:00
abstract：:Transcription factor FoxA1 plays a critical role during embryonic development and is activated during retinoic acid (RA)-induced neural differentiation of pluripotent P19 embryonal carcinoma cells at the early stage, which is marked by decreased expression of Nanog and increased expression of neural stem cell marker N...
journal_title：Stem cells and development
pub_type： 杂志文章
doi：10.1089/scd.2009.0386
更新日期：2010-09-01 00:00:00
abstract：:Balanced and precisely controlled processes between self-renewal and differentiation of hematopoietic stem cells (HSCs) into all blood lineages are critical for vertebrate definitive hematopoiesis. However, the molecular mechanisms underlying the maintenance and differentiation of HSCs have not been fully elucidated. ...
journal_title：Stem cells and development
pub_type： 杂志文章
doi：10.1089/scd.2012.0623
更新日期：2013-09-15 00:00:00
abstract：:Since human mesenchymal stem cells (MSCs) are therapeutically attractive for tissue regeneration and repair, we examined the physiological responses of human umbilical cord blood-derived MSCs (hUCB-MSCs) to genotoxic stress. We found that that sublethal doses of reactive oxygen species (ROS) and ionizing radiation cau...
journal_title：Stem cells and development
pub_type： 杂志文章
doi：10.1089/scd.2011.0284
更新日期：2012-07-20 00:00:00
abstract：:Extracellular stresses influence transcription factor (TF) expression and therefore lineage identity in the peri-implantation mouse embryo and its stem cells. This potentially affects pregnancy outcome. To understand the effects of stress signaling during this critical period of pregnancy, we exposed cultured murine e...
journal_title：Stem cells and development
pub_type： 杂志文章
doi：10.1089/scd.2014.0157
更新日期：2014-12-15 00:00:00
abstract：:In recent years it has been hypothesized that cancer stem cells (CSCs) are the actual driving force of tumor formation, highlighting the need to specifically target CSCs to successfully eradicate cancer growth and recurrence. Particularly, the deregulation of physiological signaling pathways controlling stem cell prol...
journal_title：Stem cells and development
pub_type： 杂志文章,评审
doi：10.1089/scd.2015.0081
更新日期：2015-10-15 00:00:00
abstract：:Resistance to imatinib can occur in patients with chronic myelogenous leukemia (CML). In this study, we report mechanisms of action of histone deacetylase (HDAC) inhibitor, depsipeptide (FK228) in BCR/ABL-expressing cell lines and its effectiveness in imatinib-resistant cells from patients with blast crisis of CML. FK...
journal_title：Stem cells and development
pub_type： 杂志文章
doi：10.1089/scd.2007.9994
更新日期：2007-06-01 00:00:00
abstract：:Embryonic stem-like (ES-like) cells have now been derived from the inner cell mass (ICM) of horse embryos at the blastocyst stage. Because they have been shown to express cell-surface antigens found in both human and mouse ES cells, the present study investigated gene expression patterns in day-7 horse blastocysts fro...
journal_title：Stem cells and development
pub_type： 杂志文章
doi：10.1089/scd.2007.0032
更新日期：2007-10-01 00:00:00
abstract：:Many of the currently established human embryonic stem (hES) cell lines have been characterized extensively in terms of their gene expression profiles and genetic stability in culture. Recent studies have indicated that microRNAs (miRNAs), a class of noncoding small RNAs that participate in the regulation of gene expr...
journal_title：Stem cells and development
pub_type： 杂志文章
doi：10.1089/scd.2007.0026
更新日期：2007-12-01 00:00:00
abstract：:Hematopoietic stem cells (HSCs) possess a distinct ability to perpetuate through self-renewal and to generate progeny that differentiate into mature cells of myeloid and lymphoid lineages. A better understanding of the molecular mechanisms by which HSCs replicate and differentiate from the perspective of developing ne...
journal_title：Stem cells and development
pub_type： 杂志文章,评审
doi：10.1089/scd.2006.15.755
更新日期：2006-12-01 00:00:00
abstract：:Induced pluripotent stem cells (iPSCs) are somatic cells that have been reprogrammed to a pluripotent state via introduction of defined transcription factors. iPSCs are a valuable resource for regenerative medicine, but whether iPSCs are identical to embryonic stem cells (ESCs) remains unclear. In this study, we perfo...
journal_title：Stem cells and development
pub_type： 杂志文章
doi：10.1089/scd.2011.0243
更新日期：2012-05-20 00:00:00
abstract：:In the central nervous system (CNS), generation of phenotypic diversity within the neuronal lineage is precisely regulated in a spatial and temporal fashion. Neural basic helix-loop-helix (bHLH) transcription factors are cell intrinsic factors that control commitment to neuronal lineage and play an important role in n...
journal_title：Stem cells and development
pub_type： 杂志文章
doi：10.1089/scd.2006.0115
更新日期：2007-08-01 00:00:00
abstract：:Retinoic acid (RA) is present at sites of neurogenesis in both the embryonic and adult brain. While it is widely accepted that RA signaling is involved in the regulation of neural stem cell differentiation, little is known about vitamin A utilization and biosynthesis of active retinoids in the neurogenic niches, or ab...
journal_title：Stem cells and development
pub_type： 杂志文章
doi：10.1089/scd.2012.0422
更新日期：2013-10-15 00:00:00
abstract：:Fertility clinics worldwide routinely produce a large volume of 'waste' follicular aspirate, which is potentially an abundant source of immature ovarian follicles. Current attempts to cultivate these further in vitro to yield viable mature oocytes for fertility treatment have not yet achieved much success. Instead, re...
journal_title：Stem cells and development
pub_type： 杂志文章
doi：10.1089/scd.2005.14.11
更新日期：2005-02-01 00:00:00
abstract：:Placental abnormalities can cause Pregnancy-Associated Disorders, including preeclampsia, intrauterine growth restriction, and placental insufficiency, resulting in complications for both the mother and fetus. Trophoblast cells within the labyrinthine layer of the placenta facilitate the exchange of nutrients, gases, ...
journal_title：Stem cells and development
pub_type： 杂志文章
doi：10.1089/scd.2016.0252
更新日期：2017-06-01 00:00:00
abstract：:Osteophytes are a distinct feature of osteoarthritis (OA). Their formation may be related to pluripotential cells in the periosteum responding to stimulus during OA. This study aimed to isolate stem cells from osteophyte tissues and to characterize their phenotype, proliferation, and differentiation potential, as well...
journal_title：Stem cells and development
pub_type： 杂志文章
doi：10.1089/scd.2007.0146
更新日期：2008-04-01 00:00:00
abstract：:We have previously isolated mesenchymal stromal cells (MSCs) from the tracheal aspirates of premature neonates with respiratory distress. Although isolation of MSCs correlates with the development of bronchopulmonary dysplasia, the physiologic role of these cells remains unclear. To address this, we further characteri...
journal_title：Stem cells and development
pub_type： 杂志文章
doi：10.1089/scd.2010.0494
更新日期：2011-11-01 00:00:00
abstract：:The AMP-activated protein kinase (AMPK) mediates rapid, stress-induced loss of the inhibitor of differentiation (Id)2 in blastocysts and trophoblast stem cells (TSC), and a lasting differentiation in TSC. However, it is not known if AMPK regulates other potency factors or regulates them before the blastocyst stage. Th...
journal_title：Stem cells and development
pub_type： 杂志文章
doi：10.1089/scd.2012.0352
更新日期：2013-05-15 00:00:00
abstract：:Human embryonic stem (hES) cells, like other stem cells, have the capacity to self-renew without differentiation. Although hES cells can be differentiated to many different tissue types in vitro, clinical uses have not yet been realized from the study of hES cells. Anticipation that these cells would be immediately us...
journal_title：Stem cells and development
pub_type： 杂志文章,评审
doi：10.1089/154732804323099172
更新日期：2004-06-01 00:00:00
abstract：:In the past few years research on stem cells has exploded as a tool to develop potential therapies to treat incurable neurodegenerative diseases. Stem cell transplantation has been effective in several animal models, but the underlying restorative mechanisms are still unknown. Several events such as cell fusion, neuro...
journal_title：Stem cells and development
pub_type： 杂志文章,评审
doi：10.1089/154732804773099326
更新日期：2004-02-01 00:00:00
abstract：:Heterotopic ossification (HO) is a common and debilitating complication of burns, traumatic brain injuries, and musculoskeletal trauma and surgery. Although the exact mechanism of ectopic bone formation is unknown, mesenchymal stem cells (MSCs) capable of osteogenic differentiation are known to play an essential role....
journal_title：Stem cells and development
pub_type： 杂志文章
doi：10.1089/scd.2014.0291
更新日期：2015-01-15 00:00:00
abstract：:Here, we describe a focused microarray for screening rat embryonic stem cells (ESCs) and provide validation data that this array can distinguish undifferentiated rat ESCs from rat trophoblast stem (TS) cells, rat extraembryonic endoderm cells, mouse embryonic fibroblast feeder cells, and differentiated rat ESCs. Using...
journal_title：Stem cells and development
pub_type： 杂志文章
doi：10.1089/scd.2012.0279
更新日期：2013-02-01 00:00:00
