Neurospheres derived from human embryoid bodies treated with retinoic Acid show an increase in nestin and ngn2 expression that correlates with the proportion of tyrosine hydroxylase-positive cells.

abstract：:We have isolated a novel progenitor cell population from adult rat pancreatic ducts, termed pancreatic-derived progenitor cells (PDPCs). Here, we report the in vitro culture, selection, and characterization of Thy1.1-positive and Thy1.1-negative PDPC subpopulations. These cells exhibit bipotentiality for differentiati...

journal_title：Stem cells and development

authors： Stevenson KS,McGlynn L,Hodge M,McLinden H,George WD,Davies RW,Shiels PG

更新日期：2009-12-01 00:00:00

abstract：:Improvement in angiogenesis using mesenchymal stem cells (MSCs) is evolving as an option in patients with vascular insufficiencies. The paracrine factors secreted by MSCs have been attributed to the angiogenic response. This study was conducted to identify the factors secreted by umbilical cord-derived MSCs (UCMSCs) t...

journal_title：Stem cells and development

authors： Kuchroo P,Dave V,Vijayan A,Viswanathan C,Ghosh D

更新日期：2015-02-15 00:00:00

abstract：:Stem cell therapies show great medical promise, but few new products have made it into the marketplace. The translation of stem and other cell therapies faces not only challenges associated with research and development, but also the challenges of investment funding and regulatory approval. Regulators and investors al...

journal_title：Stem cells and development

authors： Plant AL,Parker GC

更新日期：2013-09-15 00:00:00

abstract：:Mesenchymal stem cells (MSCs) have received great attention due to their remarkable regenerative, angiogenic, antiapoptotic, and immunosuppressive properties. Although conventionally isolated from the bone marrow, they are known to exist in all tissues and organs, raising the question on whether they are identical cel...

journal_title：Stem cells and development

authors： Nora CC,Camassola M,Bellagamba B,Ikuta N,Christoff AP,Meirelles Lda S,Ayres R,Margis R,Nardi NB

更新日期：2012-07-01 00:00:00

abstract：:Mesenchymal stem cells (MSCs) have a superior immunomodulatory capacity compared to other cells of the immune system, and they hold great promise for treating various immune disorders. However, their regulatory effects on the maturation of immature dendritic cells (imDCs) are not fully understood. In this study, we sh...

journal_title：Stem cells and development

authors： Liu X,Ren S,Ge C,Cheng K,Li X,Zhao RC

更新日期：2018-04-15 00:00:00

abstract：:Chronic idiopathic neutropenia (CIN) is a granulopoiesis disorder associated with an inhibitory bone marrow (BM) microenvironment consisting of activated T-lymphocytes and pro-inflammatory mediators. In this study, we investigated the possible involvement of BM mesenchymal stem cells (MSCs) in the pathophysiology of C...

journal_title：Stem cells and development

authors： Stavroulaki E,Kastrinaki MC,Pontikoglou C,Eliopoulos D,Damianaki A,Mavroudi I,Pyrovolaki K,Katonis P,Papadaki HA

更新日期：2011-08-01 00:00:00

abstract：:Acute respiratory distress syndrome (ARDS)/acute lung injury (ALI) is a severe clinical respiratory failure disorder associated with chronic pathology and disability and has a mortality rate of 40%-60%. However, the pathogenesis of ARDS/ALI remains unclear, and existing therapeutic options are insufficient for address...

journal_title：Stem cells and development

authors： Wang Y,Li H,Li X,Su X,Xiao H,Yang J

更新日期：2021-02-01 00:00:00

abstract：:The activation and transcriptional activity of signal transducer and activator of transcription 3 (STAT3) is essential for maintaining mouse embryonic stem (ES) cell cultures in an undifferentiated state. However, reports from human and monkey ES-cell culture suggest that STAT3 is dispensable for pluripotency in these...

journal_title：Stem cells and development

authors： Wagner TU,Kraeussling M,Fedorov LM,Reiss C,Kneitz B,Schartl M

更新日期：2009-01-01 00:00:00

abstract：:Recent studies have revealed that mesenchymal stem cells (MSCs) have a great potential in therapeutic applications. The low efficiency of MSC recruitment and homing to sites of diseased organ tissue, however, remains a major hurdle in their application for treatment of diseases. Stress is commonly associated with vari...

journal_title：Stem cells and development

authors： Zhang S,Lv C,Yang X,Han Z,Zhang S,Zhang J,Zong C,Gao L,Li L,Zhao Q,Li R,Yang Y,Yu F,Li X,Zhang P,Wei L

更新日期：2015-03-01 00:00:00

abstract：:Phenotypic guidance of embryonic stem (ES) cell fate is paramount if these cells are to be used for tissue repair and regeneration. Our objective was to compare two different cell culture feeders and their effect on proliferation, apoptosis, and differentiation of human (h) ES cells. HSF-6 hES cells were grown in Knoc...

journal_title：Stem cells and development

authors： Ozolek JA,Jane EP,Krowsoski L,Sammak PJ

更新日期：2007-06-01 00:00:00

abstract：:Expression of the transcription factor Snail is required for normal vasculogenesis in the developing mouse embryo. In addition, tumors expressing Snail have been associated with a more malignant phenotype, with both increased invasive properties and angiogenesis. Although the relationship between Snail and vasculogene...

journal_title：Stem cells and development

authors： Gill JG,Langer EM,Lindsley RC,Cai M,Murphy TL,Murphy KM

更新日期：2012-01-20 00:00:00

abstract：:Embryonic stem cells (ESCs) posses the ability to self-renew and differentiate into a multitude of lineages, including the osteogenic lineage in vitro. Currently, most approaches have focused on embryonic body (EB)-mediated osteogenic differentiation, which relies on formation of all three germ layers resulting in lim...

journal_title：Stem cells and development

authors： Hwang YS,Polak JM,Mantalaris A

更新日期：2008-10-01 00:00:00

abstract：:Pluripotent stem cell-derived endothelial cells (ECs) have great potential to be used in vascular therapy or tissue engineering. It is also much desired to obtain arterial or venous ECs for specific applications. Factors that are critical for the proper arterial or venous differentiation from pluripotent stem cells st...

journal_title：Stem cells and development

authors： Kim D,Lee V,Dorsey TB,Niklason LE,Gui L,Dai G

更新日期：2018-04-01 00:00:00

abstract：:Cell therapy with adult mesenchymal stem cells (MSCs) is a promising approach to regenerative medicine and autoimmune diseases. There are various approaches to improve the efficacy of MSC-based therapeutics, and MSC preparation as spheroidal aggregates, or MSC spheroids, is a novel preparatory and delivery method. Sph...

journal_title：Stem cells and development

authors： Cesarz Z,Funnell JL,Guan J,Tamama K

更新日期：2016-04-15 00:00:00

abstract：:Small molecules (SM) can greatly enhance the efficiency of induced pluripotent stem (iPS) cell generation, but the mechanisms by which they act have not been fully explored. We show here that an SM cocktail (NaB, PD03259, and SB431542) significantly promotes iPS cell generation from human fibroblasts, and NaB is more ...

journal_title：Stem cells and development

authors： Zhang Z,Wu WS

更新日期：2013-08-15 00:00:00

abstract：:Embryonic stem-like (ES-like) cells have now been derived from the inner cell mass (ICM) of horse embryos at the blastocyst stage. Because they have been shown to express cell-surface antigens found in both human and mouse ES cells, the present study investigated gene expression patterns in day-7 horse blastocysts fro...

journal_title：Stem cells and development

authors： Guest DJ,Allen WR

更新日期：2007-10-01 00:00:00

abstract：:We determined whether cartilage could be engineered from mesenchymal progenitor cells (MPCs) normally found in amniotic fluid. Mesenchymal amniocytes were isolated from ovine amniotic fluid samples (n = 5) and had their identity confirmed by immunocytochemistry. Cells were expanded and then cultured as micromass pelle...

journal_title：Stem cells and development

authors： Kunisaki SM,Jennings RW,Fauza DO

更新日期：2006-04-01 00:00:00

abstract：:We describe a method of generating an enriched population of NCAM-positive cells from a human teratocarcinoma cell line (NTera2/D1) and their differentiation into midbrain dopaminergic neurons in the absence of the caudalizing factor retinoic acid (RA). NTera2 cells were induced to form embryoid bodies and then to gen...

journal_title：Stem cells and development

authors： Ravindran G,Rao HS

更新日期：2006-08-01 00:00:00

abstract：:The central role of calcium influx and electrical activity in embryonic development raises important questions about the role and regulation of voltage-dependent calcium influx. Using cultured neural progenitor cell (NPC) preparations, we recorded barium currents through voltage-activated channels using the whole-cell...

journal_title：Stem cells and development

authors： Louhivuori LM,Louhivuori V,Wigren HK,Hakala E,Jansson LC,Nordström T,Castrén ML,Akerman KE

更新日期：2013-04-15 00:00:00

abstract：:Tissue engineering and cell implantation therapies are gaining popularity because of their potential to repair and regenerate tissues and organs. To investigate the role of inflammatory cytokines in new tissue development in engineered tissues, we have characterized the nature and timing of cell populations forming ne...

journal_title：Stem cells and development

authors： Lilja HE,Morrison WA,Han XL,Palmer J,Taylor C,Tee R,Möller A,Thompson EW,Abberton KM

更新日期：2013-05-15 00:00:00

abstract：:Occupational asthma (OA) is characterized by allergic airway inflammation and hyperresponsiveness, leading to progressive airway remodeling and a concomitant decline in lung function. The management of OA remains suboptimal in clinical practice. Thus, establishing effective therapies might overcome the natural history...

journal_title：Stem cells and development

authors： Martínez-González I,Cruz MJ,Moreno R,Morell F,Muñoz X,Aran JM

更新日期：2014-10-01 00:00:00

abstract：:The aim of this study was to clarify the mechanisms that regulate hematopoietic cell expansion in vitro by identifying defined culture conditions. We report the results of experiments with CD34(+) cells from cord blood (CB, n = 13), bone marrow (BM, n = 4), and mobilized peripheral blood stem cells (PBSC, n = 5) using...

journal_title：Stem cells and development

authors： Rossi B,Zanolin E,Vincenzi C,Diani F,Pizzolo G,de Wynter E,Nadali G

更新日期：2004-08-01 00:00:00

abstract：:Human perivascular progenitor cells, including pericytes, are well-described multipotent mesenchymal cells giving rise to mesenchymal stem cells in culture. Despite the unique location of pericytes, specific antigens to distinguish human pericytes from other cell types are few. Here, we employed a human tissue microar...

journal_title：Stem cells and development

authors： Hsu CY,Salazar MG,Miller S,Meyers C,Ding C,Hardy W,Péault B,James AW

更新日期：2019-09-15 00:00:00

abstract：:How hematopoietic stem cells (HSCs) maintain the balance of self-renewal and differentiation could be partially ascribed to asymmetric and symmetric division patterns. However, a simple and effective method to detect stem cell division patterns is lacking. In this study, we introduce a strategy to describe stem cells ...

journal_title：Stem cells and development

authors： Yang WZ,Yu WY,Chen T,Wang XF,Dong F,Xie ME,Gong YM,Liang HY,Fu WC

更新日期：2019-07-15 00:00:00

abstract：:Embryonic stem cells (ESCs) have the capacity to form all the tissues in the body and hence, directed differentiation of ESCs along specific lineages represents a means to generate therapeutically useful cell types. It has been postulated that, during in vitro differentiation, ES cells sequentially pass through simila...

journal_title：Stem cells and development

authors： Mossman AK,Sourris K,Ng E,Stanley EG,Elefanty AG

更新日期：2005-12-01 00:00:00

abstract：:Embryonic stem (ES) cells are recognized as an excellent cell culture model for studying developmental mechanisms and their therapeutic modulations. The aim of this work was to define whether using magnetofection was an efficient way to manipulate stem cells genetically without adversely affecting their proliferation ...

journal_title：Stem cells and development

authors： Lee CH,Kim EY,Jeon K,Tae JC,Lee KS,Kim YO,Jeong MY,Yun CW,Jeong DK,Cho SK,Kim JH,Lee HY,Riu KZ,Cho SG,Park SP

更新日期：2008-02-01 00:00:00

abstract：:Extracellular stresses influence transcription factor (TF) expression and therefore lineage identity in the peri-implantation mouse embryo and its stem cells. This potentially affects pregnancy outcome. To understand the effects of stress signaling during this critical period of pregnancy, we exposed cultured murine e...

journal_title：Stem cells and development

authors： Slater JA,Zhou S,Puscheck EE,Rappolee DA

更新日期：2014-12-15 00:00:00

abstract：:Human umbilical cord blood (CB) has been used successfully in stem cell transplantation. A subpopulation of CD34(+) cells expresses chemokine receptor CXCR4 which is critical for bone marrow engraftment in human hematopoietic stem cells. Here, we demonstrate the effect of short-term culture on CXCR4 expression on umbi...

journal_title：Stem cells and development

authors： Ohno N,Kajiume T,Sera Y,Sato T,Kobayashi M

更新日期：2009-10-01 00:00:00

abstract：:Human embryonic stem cells (hESCs) are derived from the inner cell mass (ICM) of the blastocyst and can differentiate into any cell type in the human body. These cells hold a great potential for regenerative medicine, but to obtain enough cells needed for medical treatment, culture is required on a large scale. In the...

journal_title：Stem cells and development

authors： Jensen PL,Beck HC,Petersen J,Hreinsson J,Wånggren K,Laursen SB,Sørensen PD,Christensen ST,Andersen CY

更新日期：2013-04-01 00:00:00

abstract：:To develop biological approaches to restore vision, we developed a method of transplanting stem cell-derived retinal tissue into the subretinal space of a large-eye animal model (cat). Human embryonic stem cells (hESC) were differentiated to retinal organoids in a dish. hESC-derived retinal tissue was introduced into ...

journal_title：Stem cells and development

authors： Singh RK,Occelli LM,Binette F,Petersen-Jones SM,Nasonkin IO

更新日期：2019-09-01 00:00:00