Translating stem cell research from the bench to the clinic: a need for better quality data.

abstract：:Ossification of the posterior longitudinal ligament (OPLL) is characterized by ectopic OPLL. To date, the specific molecular pathogenesis of OPLL has not been clearly elucidated. In this study, bone marrow-derived mesenchymal stem cells obtained from healthy donors (HD-MSCs) and patients with OPLL (OPLL-MSCs) were cul...

journal_title：Stem cells and development

authors： Cai Z,Wu B,Ye G,Liu W,Chen K,Wang P,Xie Z,Li J,Zheng G,Yu W,Su Z,Lin J,Wu Y,Shen H

更新日期：2020-12-01 00:00:00

abstract：:Expression of the transcription factor Snail is required for normal vasculogenesis in the developing mouse embryo. In addition, tumors expressing Snail have been associated with a more malignant phenotype, with both increased invasive properties and angiogenesis. Although the relationship between Snail and vasculogene...

journal_title：Stem cells and development

authors： Gill JG,Langer EM,Lindsley RC,Cai M,Murphy TL,Murphy KM

更新日期：2012-01-20 00:00:00

abstract：:Body axes and germ layers evolve at gastrulation, and in mammals are driven by many genes; however, what orchestrates the genetic pathways during gastrulation remains elusive. Previously, we presented evidence that microRNA-17 (miRNA-17) family members, miR-17-5p, miR-20a, miR-93, and miR-106a were differentially expr...

journal_title：Stem cells and development

authors： Larabee SM,Coia H,Jones S,Cheung E,Gallicano GI

更新日期：2015-02-01 00:00:00

abstract：:We describe a rapid and efficient 5-step program of defined factors for the genesis of brain myelin-forming oligodendrocytes (OLs) from embryonic stem cells (ESCs). The OLs emerge on the same time frame in vitro as seen in vivo. Factors promoting neural induction (retinoids, noggin) are required, while exogenous Sonic...

journal_title：Stem cells and development

authors： Sadowski D,Kiel ME,Apicella M,Arriola AG,Chen CP,McKinnon RD

更新日期：2010-09-01 00:00:00

abstract：:We have isolated a novel progenitor cell population from adult rat pancreatic ducts, termed pancreatic-derived progenitor cells (PDPCs). Here, we report the in vitro culture, selection, and characterization of Thy1.1-positive and Thy1.1-negative PDPC subpopulations. These cells exhibit bipotentiality for differentiati...

journal_title：Stem cells and development

authors： Stevenson KS,McGlynn L,Hodge M,McLinden H,George WD,Davies RW,Shiels PG

更新日期：2009-12-01 00:00:00

abstract：:Regenerative medicine, a paragon of future healthcare, holds unprecedented potential in extending the reach of treatment modalities for individuals across diseases and lifespan. Emerging regenerative technologies, focused on structural repair and functional restoration, signal a radical transformation in medical and s...

journal_title：Stem cells and development

authors： Terzic A,Harper CM Jr,Gores GJ,Pfenning MA

更新日期：2013-12-01 00:00:00

abstract：:Adipose-derived stem cells (ADSCs) are a type of multipotent mesenchymal stem cells with immunosuppressive capacities. However, the underlying mechanisms involved in the inhibitory effects of ADSCs on T cells are not completely elucidated. In this study, human peripheral blood mononuclear cells (PBMCs) stimulated with...

journal_title：Stem cells and development

authors： Zhou K,Guo S,Tong S,Sun Q,Li F,Zhang X,Qiao Y,Liang G

更新日期：2018-09-01 00:00:00

abstract：:Mesenchymal stem cells (MSC) possess a range of immunomodulatory properties which they exert through soluble mediators and through direct cell-cell contact. Due to these immune regulatory properties, the safety and clinical efficacy of MSC treatment has been tested in a number of autoimmune disorders. In this review w...

journal_title：Stem cells and development

authors： Munir H,McGettrick HM

更新日期：2015-09-15 00:00:00

abstract：:Multipotent bone marrow-derived mesenchymal stem/stromal cells (BMSCs) exhibit a finite life span after ex vivo expansion leading to cellular senescence. Many factors can contribute to this. Recently, our group has identified for the first time expression of the chemokine-like factor superfamily 8 (CMTM8) gene in cult...

journal_title：Stem cells and development

authors： H'ng CH,Camp E,Anderson PJ,Zannettino ACW,Gronthos S

更新日期：2020-07-01 00:00:00

abstract：:Citrin deficiency (CD) is a recessive genetic disorder caused by mutations in the citrin gene SLC25A13. CD causes various symptoms related to nutrient metabolism such as urea cycle failure, abnormal amino acid levels, and fatty liver. To understand the pathophysiology of CD, the molecular phenotypes were investigated ...

journal_title：Stem cells and development

authors： Kim Y,Choi JY,Lee SH,Lee BH,Yoo HW,Han YM

更新日期：2016-04-15 00:00:00

abstract：:Induced pluripotent stem cell (iPSC) reprogramming requires sustained expression of multiple reprogramming factors for a limited period of time (10-30 days). Conventional iPSC reprogramming was achieved using lentiviral or simple retroviral vectors. Retroviral reprogramming has flaws of insertional mutagenesis, uncont...

journal_title：Stem cells and development

authors： Hu K

更新日期：2014-06-15 00:00:00

abstract：:Mesenchymal stem cells (MSCs) can be mobilized from the bone marrow and enter the circulation. Conversely, MSCs can be recruited from the circulation and into the bone marrow. For these migratory pathways, MSCs have to traverse the bone marrow endothelium, in a basal-to-apical and apical-to-basal direction, respective...

journal_title：Stem cells and development

authors： Smith H,Whittall C,Weksler B,Middleton J

更新日期：2012-02-10 00:00:00

abstract：:Stem cell-based therapies have demonstrated great potential for the treatment of cardiac diseases, for example, myocardial infarction; however, low cell viability, low retention/engraftment, and uncontrollable in vivo differentiation after transplantation are still major limitations, which lead to low therapeutic effi...

journal_title：Stem cells and development

authors： Ledford BT,Simmons J,Chen M,Fan H,Barron C,Liu Z,Van Dyke M,He JQ

更新日期：2017-06-15 00:00:00

abstract：:With the recent advances in regenerative medicine, nanotechnology has created a niche for itself as a promising avenue in this field. Innumerable studies have been carried out by researchers using virus-based methodologies for the purpose of epigenetic reprogramming. Although this method is ostensibly safe, nonetheles...

journal_title：Stem cells and development

authors： Kadengodlu PA,Rai VR

更新日期：2015-03-01 00:00:00

abstract：:Members of the homeobox (Hox) gene family are known to mediate expansion of hematopoietic stem cells (HSCs) and progenitors. The absence of oncogenic properties promoted HOXB4 as prime candidate in the quest to expand HSCs for clinical purposes. Despite its potential to expand HSCs, studies with mutant mice showed tha...

journal_title：Stem cells and development

authors： Fournier M,Lebert-Ghali CÉ,Krosl G,Bijl JJ

更新日期：2012-01-01 00:00:00

abstract：:While transplantation of human induced pluripotent stem cell-derived neural stem cells (hiPSC-NSCs) shows therapeutic potential in animal stroke models, major concerns for translating hiPSC therapy to the clinic are efficacy and safety. Therefore, there is a demand to develop an optimal strategy to enhance the engraft...

journal_title：Stem cells and development

authors： Ould-Brahim F,Sarma SN,Syal C,Lu KJ,Seegobin M,Carter A,Jeffers MS,Doré C,Stanford WL,Corbett D,Wang J

更新日期：2018-08-15 00:00:00

abstract：:Traditionally, undifferentiated pluripotent human embryonic and induced pluripotent stem cells (hESCs and hiPSCs) have been expanded as monolayer colonies in adhesion culture, both in the presence or absence of feeder cells. However, the use of pluripotent stem cells poses the need to scale-up current culture methods....

journal_title：Stem cells and development

authors： Larijani MR,Seifinejad A,Pournasr B,Hajihoseini V,Hassani SN,Totonchi M,Yousefi M,Shamsi F,Salekdeh GH,Baharvand H

更新日期：2011-11-01 00:00:00

abstract：:There is surprising confusion surrounding the concept of biological totipotency, both within the scientific community and in society at large. Increasingly, ethical objections to scientific research have both practical and political implications. Ethical controversy surrounding an area of research can have a chilling ...

journal_title：Stem cells and development

authors： Condic ML

更新日期：2014-04-15 00:00:00

abstract：:A long-term goal of mesenchymal progenitor cell (MPC) research is to identify cell-surface markers to facilitate MPC isolation. One reported MPC feature in humans and other species is lack of CD14 (lipopolysaccharide receptor) expression. The aim of this study was to evaluate CD14 as an MPC sorting marker. Our hypothe...

journal_title：Stem cells and development

authors： Hackett CH,Flaminio MJ,Fortier LA

更新日期：2011-04-01 00:00:00

abstract：:Human umbilical cord blood (CB) has been used successfully in stem cell transplantation. A subpopulation of CD34(+) cells expresses chemokine receptor CXCR4 which is critical for bone marrow engraftment in human hematopoietic stem cells. Here, we demonstrate the effect of short-term culture on CXCR4 expression on umbi...

journal_title：Stem cells and development

authors： Ohno N,Kajiume T,Sera Y,Sato T,Kobayashi M

更新日期：2009-10-01 00:00:00

abstract：:Stromal cells have been used to induce dopaminergic differentiation of mouse, primate, and human embryonic stem cells (hESCs), but the mechanism that governs this induction is unknown. In this manuscript, we show that medium conditioned by the stromal cell line PA6 (PA6-CM) can induce dopaminergic differentiation in n...

journal_title：Stem cells and development

authors： Swistowska AM,da Cruz AB,Han Y,Swistowski A,Liu Y,Shin S,Zhan M,Rao MS,Zeng X

更新日期：2010-01-01 00:00:00

abstract：:Improvement in angiogenesis using mesenchymal stem cells (MSCs) is evolving as an option in patients with vascular insufficiencies. The paracrine factors secreted by MSCs have been attributed to the angiogenic response. This study was conducted to identify the factors secreted by umbilical cord-derived MSCs (UCMSCs) t...

journal_title：Stem cells and development

authors： Kuchroo P,Dave V,Vijayan A,Viswanathan C,Ghosh D

更新日期：2015-02-15 00:00:00

abstract：:Extraembryonic endoderm (XEN) cells are stem cell lines derived from primitive endoderm cells of inner cell mass in blastocysts. These cells have self-renewal properties and differentiate into visceral endoderm (VE) and parietal endoderm (PE) of the yolk sac. Recently, it has been reported that XEN cells can contribut...

journal_title：Stem cells and development

authors： Nishimura T,Unezaki N,Kanegi R,Wijesekera DPH,Hatoya S,Sugiura K,Kawate N,Tamada H,Imai H,Inaba T

更新日期：2017-08-01 00:00:00

abstract：:Small-molecule inhibitors of glycogen synthase kinase 3β (GSK3β) have demonstrated strong anti-leukemia effects in preclinical studies. Here, we investigated the effect of GSK3β inhibitor 6-Bromoindirubin-3-oxime (BIO) previously shown to inhibit leukemia cell growth in vitro and of animal models on hematopoietic rege...

journal_title：Stem cells and development

authors： Shen S,Xu N,Klamer G,Ko KH,Khoo M,Ma D,Moore J,O'Brien TA,Dolnikov A

更新日期：2015-03-15 00:00:00

abstract：:Periodontitis is an infectious disease characterized by chronic inflammation and progressive destruction of periodontal tissues. Chronic inflammatory environment may affect immunomodulatory function of periodontal ligament stem cells (PDLSCs) and promote shift toward proinflammatory phenotype contributing to propagati...

journal_title：Stem cells and development

authors： Čebatariūnienė A,Kriaučiūnaitė K,Prunskaitė J,Tunaitis V,Pivoriūnas A

更新日期：2019-08-01 00:00:00

abstract：:In the past few years research on stem cells has exploded as a tool to develop potential therapies to treat incurable neurodegenerative diseases. Stem cell transplantation has been effective in several animal models, but the underlying restorative mechanisms are still unknown. Several events such as cell fusion, neuro...

journal_title：Stem cells and development

authors： Cova L,Ratti A,Volta M,Fogh I,Cardin V,Corbo M,Silani V

更新日期：2004-02-01 00:00:00

abstract：:Human embryonic stem (hES) cells represent a potentially unlimited source of transplantable beta-cells for the treatment of diabetes. Here we describe a differentiation strategy that reproducibly directs HES3, an National Institutes of Health (NIH)-registered hES cell line, into cells of the pancreatic endocrine linea...

journal_title：Stem cells and development

authors： Phillips BW,Hentze H,Rust WL,Chen QP,Chipperfield H,Tan EK,Abraham S,Sadasivam A,Soong PL,Wang ST,Lim R,Sun W,Colman A,Dunn NR

更新日期：2007-08-01 00:00:00

abstract：:Here, we describe a focused microarray for screening rat embryonic stem cells (ESCs) and provide validation data that this array can distinguish undifferentiated rat ESCs from rat trophoblast stem (TS) cells, rat extraembryonic endoderm cells, mouse embryonic fibroblast feeder cells, and differentiated rat ESCs. Using...

journal_title：Stem cells and development

authors： Hong J,He H,Bui P,Ryba-White B,Rumi MA,Soares MJ,Dutta D,Paul S,Kawamata M,Ochiya T,Ying QL,Rajanahalli P,Weiss ML

更新日期：2013-02-01 00:00:00

abstract：:Studies on rodent bone marrow stromal cells (MSCs) have revealed a capacity, for at least a portion of cells, to express neuron-like traits after differentiation in culture. Little, however, is known about the ability of human MSCs in this regard. We show here that incubation with certain differentiation cocktails, pa...

journal_title：Stem cells and development

authors： Suon S,Jin H,Donaldson AE,Caterson EJ,Tuan RS,Deschennes G,Marshall C,Iacovitti L

更新日期：2004-12-01 00:00:00

abstract：:The differentiation of human pluripotent stem cells to the B-cell lymphoid lineage has important clinical applications that include in vitro modeling of developmental lymphogenesis in health and disease. Here, we first demonstrate the capacity of human induced pluripotent stem cells (hiPSCs) to differentiate into CD14...

journal_title：Stem cells and development

authors： French A,Yang CT,Taylor S,Watt SM,Carpenter L

更新日期：2015-05-01 00:00:00