Abstract:
:We describe a rapid and efficient 5-step program of defined factors for the genesis of brain myelin-forming oligodendrocytes (OLs) from embryonic stem cells (ESCs). The OLs emerge on the same time frame in vitro as seen in vivo. Factors promoting neural induction (retinoids, noggin) are required, while exogenous Sonic hedgehog is not. In contrast we were unable to generate OLs by trans-differentiation of ethically neutral mesenchymal stem cells, indicating a requirement for cis-differentiation via neural ectoderm for OL genesis. In the ESC-derived cultures, our optimized protocol generated a mixed population with 49% O4(+), Olig2(+) OL lineage cells. These cultures also retained pluripotential markers including Oct4, and an analysis of embryoid body formation in vitro, and allogeneic grafts in vivo, revealed that the ESC-derived cultures also retained teratogenic cells. The frequency of embryoid body formation from terminal differentiated OL cultures was 0.001%, 100-fold lower than that from ESCs. Our results provide the first quantitative measurement of teratogenicity in ESC-derived, exhaustively differentiated allogeneic grafts, and demonstrate the unequivocal need to purify ESC-derived cells in order to generate a safe population for regenerative therapy.
journal_name
Stem Cells Devjournal_title
Stem cells and developmentauthors
Sadowski D,Kiel ME,Apicella M,Arriola AG,Chen CP,McKinnon RDdoi
10.1089/scd.2009.0520subject
Has Abstractpub_date
2010-09-01 00:00:00pages
1343-53issue
9eissn
1547-3287issn
1557-8534journal_volume
19pub_type
杂志文章abstract::Uterine remodeling during pregnancy is a fundamental, dynamic process required for successful propagation of eutherian species. The uterus can increase in size up to 40-fold during pregnancy, which is largely attributed to expansion of the myometrium by hyperplasia and hypertrophy. After pregnancy, the uterus repairs ...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2018.0146
更新日期:2018-12-15 00:00:00
abstract::The secretome of human amniotic fluid stem cells (AFSCs) has great potential as a therapeutic agent in regenerative medicine. However, it must be produced in a clinically compliant manner before it can be used in humans. In this study, we developed a means of producing a biologically active secretome from AFSCs that i...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2017.0089
更新日期:2017-09-15 00:00:00
abstract::Rare heterozygous deletions in the neurexin 1 (NRXN1) gene robustly increase an individual's risk of developing neurological and psychiatric disorders. However, the molecular bases by which different mutations result in different clinical presentations, with variable penetrance, are unknown. To better understand the m...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2020.0017
更新日期:2020-09-01 00:00:00
abstract::Human perivascular progenitor cells, including pericytes, are well-described multipotent mesenchymal cells giving rise to mesenchymal stem cells in culture. Despite the unique location of pericytes, specific antigens to distinguish human pericytes from other cell types are few. Here, we employed a human tissue microar...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2019.0106
更新日期:2019-09-15 00:00:00
abstract::Studies on rodent bone marrow stromal cells (MSCs) have revealed a capacity, for at least a portion of cells, to express neuron-like traits after differentiation in culture. Little, however, is known about the ability of human MSCs in this regard. We show here that incubation with certain differentiation cocktails, pa...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2004.13.625
更新日期:2004-12-01 00:00:00
abstract::Myocardial infarction is a major public health problem that causes significant mortality despite recent advances in its prevention and treatment. Therefore, approaches based on adult stem cells represent a promising alternative to conventional therapies for this life-threatening condition. Mesenchymal stem cells (MSCs...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2008.0292
更新日期:2009-07-01 00:00:00
abstract::Bone morphogenetic protein-2 (BMP-2) is a novel differentiation factor that is capable of inducing osteoblast differentiation and bone formation, making it an attractive option in treatment of bone defects, fractures, and spine fusions. Inflammation, which was a common situation during bone healing, is recognized to i...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2013.0345
更新日期:2014-02-01 00:00:00
abstract::Dental follicle stem cells (DFSCs), which are the stem cells present in the dental follicle tissue of the tooth germ and are derived from the neural crest, are direct precursor cells of periodontal tissues and can form the periodontal ligament, cementum, and alveolar bone proper in the late stage of tooth development....
journal_title:Stem cells and development
pub_type: 杂志文章,评审
doi:10.1089/scd.2019.0012
更新日期:2019-08-01 00:00:00
abstract::To be clinically relevant as a therapy for heart failure, endogenous progenitor cells must be isolated and expanded from aged and/or diseased tissue. Here, we investigated the effect of age and cardiac impairment resulting from lack of dystrophin on murine cardiosphere-derived cells (CDCs). CDCs were isolated and expa...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2013.0388
更新日期:2014-05-01 00:00:00
abstract::Electrical stimulation (ES) of cells has been shown to induce a variety of responses, such as cytoskeleton rearrangements, migration, proliferation, and differentiation. In this study, we have investigated whether monophasic and biphasic pulsed ES could exert any effect on the proliferation and differentiation of huma...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2013.0375
更新日期:2014-04-15 00:00:00
abstract::Ex vivo cultures of human bone marrow-derived mesenchymal stem cells (MSCs) contain subsets of progenitors exhibiting dissimilar properties. One of these subsets comprises uncommitted progenitors displaying distinctive features, such as morphology, a quiescent condition, growth factor production, and restricted tissue...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2005.14.408
更新日期:2005-08-01 00:00:00
abstract::Primary periodontal ligament stem cells (PDLSCs) are known to possess multidifferentiation potential and exhibit an immunophenotype similar to that described for bone-marrow-derived mesenchymal stem cells. In the present study, bromo-deoxyuridine (BrdU)-labeled ovine PDLSCs implanted into immunodeficient mice survived...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2013.0490
更新日期:2014-05-01 00:00:00
abstract::In the mammalian olfactory epithelium (OE), olfactory receptor neurons (ORNs) are continuously regenerated throughout the animal's lifetime. Horizontal basal cells (HBCs) in the OE express the epithelial marker keratin 5 (K5) and the stem cell marker Pax6 and are considered relatively quiescent tissue stem cells in th...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2015.0011
更新日期:2015-08-15 00:00:00
abstract::Mesenchymal stem cells (MSC) possess a range of immunomodulatory properties which they exert through soluble mediators and through direct cell-cell contact. Due to these immune regulatory properties, the safety and clinical efficacy of MSC treatment has been tested in a number of autoimmune disorders. In this review w...
journal_title:Stem cells and development
pub_type: 杂志文章,评审
doi:10.1089/scd.2015.0008
更新日期:2015-09-15 00:00:00
abstract::Little is known about the effects of ionizing radiation on the earliest stages of embryonic development although it is well recognized that ionizing radiation is a natural part of our environment and further exposure may occur due to medical applications. The current study addresses this issue using D3 mouse embryonic...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2015.0260
更新日期:2016-01-15 00:00:00
abstract::The central role of calcium influx and electrical activity in embryonic development raises important questions about the role and regulation of voltage-dependent calcium influx. Using cultured neural progenitor cell (NPC) preparations, we recorded barium currents through voltage-activated channels using the whole-cell...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2012.0234
更新日期:2013-04-15 00:00:00
abstract::A long-term goal of mesenchymal progenitor cell (MPC) research is to identify cell-surface markers to facilitate MPC isolation. One reported MPC feature in humans and other species is lack of CD14 (lipopolysaccharide receptor) expression. The aim of this study was to evaluate CD14 as an MPC sorting marker. Our hypothe...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2010.0175
更新日期:2011-04-01 00:00:00
abstract::The peritoneum mesothelium lines body cavities and has the same origin as ovarian surface epithelium with probable existence of peritoneum mesenchymal stem cells (PMSCs). In the present research, PMSCs were isolated from peritoneum and differentiated into ovarian cell-like cells using human follicular fluid (HFF) and ...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2018.0149
更新日期:2019-04-15 00:00:00
abstract::Citrin deficiency (CD) is a recessive genetic disorder caused by mutations in the citrin gene SLC25A13. CD causes various symptoms related to nutrient metabolism such as urea cycle failure, abnormal amino acid levels, and fatty liver. To understand the pathophysiology of CD, the molecular phenotypes were investigated ...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2015.0342
更新日期:2016-04-15 00:00:00
abstract::After intra-articular injection, synovium-derived mesenchymal stem cells (SMSCs) can adhere to damaged cartilage (a process called homing) and then repair the cartilage defect. Nonetheless, the main obstacle of the current method is the insufficient homing ratio of SMSCs, which fails to meet the requirements for carti...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2019.0137
更新日期:2020-04-01 00:00:00
abstract::The activation and transcriptional activity of signal transducer and activator of transcription 3 (STAT3) is essential for maintaining mouse embryonic stem (ES) cell cultures in an undifferentiated state. However, reports from human and monkey ES-cell culture suggest that STAT3 is dispensable for pluripotency in these...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2007.0262
更新日期:2009-01-01 00:00:00
abstract::Primordial germ cells (PGCs), the precursors of gametes, have regulatory mechanisms involving transcription factors and epigenetic modifications. The transcription factor NANOG is a key regulator of germ cell and embryonic stem cell characteristics. However, the epigenetic regulation of NANOG with a histone deacetylas...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2018.0059
更新日期:2018-09-01 00:00:00
abstract::My concept of cell differentiation involves genetic information from DNA being transcribed into mRNA proteins-morphogenes (mRNAs+ homeodomain proteins)-and stored in the ovoplasm as maternal inheritance, or cytoplasmic genetic memory. Feedback mechanism(s) allow these morphogenes to selectively unlock new genes, regul...
journal_title:Stem cells and development
pub_type: 社论
doi:10.1089/scd.2007.0139
更新日期:2008-10-01 00:00:00
abstract::Progenitor expansion during development is a highly regulated process dictating the final organ size, while expansion of specific progenitor populators can adjust the final cellular composition of the organ. Understanding factors involved in these pathways is required to develop cell-based therapies such as β-cell tra...
journal_title:Stem cells and development
pub_type: 杂志文章,评审
doi:10.1089/scd.2013.0106
更新日期:2013-11-15 00:00:00
abstract::The design of reliable biocompatible and biodegradable scaffolds remains one of the most important challenges for tissue engineering. In fact, properly designed scaffolds must display an adequate and interconnected porosity to facilitate cell spreading and colonization of the inner layers, and must release physical si...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2017.0051
更新日期:2017-10-01 00:00:00
abstract::Multipotent mesenchymal stromal cells (MSCs) are more and more frequently used to treat orthopedic injuries in horses. However, these cells are limited in their expandability and differentiation capacity. Recently, the first equine-induced pluripotent stem cell (iPSC) lines were reported by us [ 1 ]. In vitro differen...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2014.0409
更新日期:2016-01-01 00:00:00
abstract::To develop biological approaches to restore vision, we developed a method of transplanting stem cell-derived retinal tissue into the subretinal space of a large-eye animal model (cat). Human embryonic stem cells (hESC) were differentiated to retinal organoids in a dish. hESC-derived retinal tissue was introduced into ...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2019.0090
更新日期:2019-09-01 00:00:00
abstract::Bone marrow-derived mesenchymal stem cells (MSCs) possess multilineage differentiation potential and can be used for the treatment of diabetic heart failure. However, hyperglycemia can affect the function of MSCs adversely and merits the requirement for a strategy to correct this anomaly. MSCs were isolated from the t...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2009.0397
更新日期:2011-01-01 00:00:00
abstract::Although amniotic fluid cells can differentiate into several mesenchymal lineages and have been proposed as a valuable therapeutic cell source, their ability to undergo terminal neuronal differentiation remains a cause of controversy. The aim of this study was to investigate the neuronal differentiation ability of the...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2011.0281
更新日期:2012-03-20 00:00:00
abstract::Tissue engineering and cell implantation therapies are gaining popularity because of their potential to repair and regenerate tissues and organs. To investigate the role of inflammatory cytokines in new tissue development in engineered tissues, we have characterized the nature and timing of cell populations forming ne...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2012.0451
更新日期:2013-05-15 00:00:00