CMTM8 Is a Suppressor of Human Mesenchymal Stem Cell Osteogenic Differentiation and Promoter of Proliferation Via EGFR Signaling.

Abstract:

:Multipotent bone marrow-derived mesenchymal stem/stromal cells (BMSCs) exhibit a finite life span after ex vivo expansion leading to cellular senescence. Many factors can contribute to this. Recently, our group has identified for the first time expression of the chemokine-like factor superfamily 8 (CMTM8) gene in cultured human BMSCs. In this study, we examine the role of CMTM8 in BMSC proliferation, migration, and differentiation. Functional studies using siRNA-mediated knockdown of CMTM8 in human BMSCs resulted in decreased capacity to undergo proliferation and migration and an increased capacity for osteogenic differentiation in vitro. Furthermore, reduced CMTM8 levels led to a decrease in the epidermal growth factor receptor (EGFR) signaling pathway during BMSC proliferation and migration, respectively. Supportive studies using retroviral mediated enforced expression of CMTM8 in BMSC resulted in an increased capacity for proliferation and migration but a decreased osteogenic differentiation potential. Collectively, these data suggest that CMTM8 promotes BMSC proliferation and BMSC migration through the EGFR/ERK1/2 pathway. This study provides insight into novel regulatory mechanisms of human BMSC growth and cell fate determination.

journal_name

Stem Cells Dev

authors

H'ng CH,Camp E,Anderson PJ,Zannettino ACW,Gronthos S

doi

10.1089/scd.2020.0007

subject

Has Abstract

pub_date

2020-07-01 00:00:00

pages

823-834

issue

13

eissn

1547-3287

issn

1557-8534

journal_volume

29

pub_type

杂志文章
  • Glycosylated Cell Surface Markers for the Isolation of Human Cardiac Progenitors.

    abstract::The aim of stem cell therapy after cardiac injury is to replace damaged cardiac tissue. Human cardiac progenitor cells (CPCs) represent an interesting cell population for clinical strategies to treat cardiac disease and human CPC-specific antibodies would aid in the clinical implementation of cardiac progenitor-based ...

    journal_title:Stem cells and development

    pub_type: 杂志文章

    doi:10.1089/scd.2017.0048

    authors: Moerkamp AT,Leung HW,Bax NAM,Holst S,Lodder K,Berends T,Dingenouts CKE,Choo A,Smits AM,Goumans MJ

    更新日期:2017-11-01 00:00:00

  • Hypoxic Preconditioning of Human Umbilical Cord Mesenchymal Stem Cells Is an Effective Strategy for Treating Acute Lung Injury.

    abstract::Acute respiratory distress syndrome (ARDS)/acute lung injury (ALI) is a severe clinical respiratory failure disorder associated with chronic pathology and disability and has a mortality rate of 40%-60%. However, the pathogenesis of ARDS/ALI remains unclear, and existing therapeutic options are insufficient for address...

    journal_title:Stem cells and development

    pub_type: 杂志文章

    doi:10.1089/scd.2020.0174

    authors: Wang Y,Li H,Li X,Su X,Xiao H,Yang J

    更新日期:2021-02-01 00:00:00

  • Generation of Distal Airway Epithelium from Multipotent Human Foregut Stem Cells.

    abstract::Collectively, lung diseases are one of the largest causes of premature death worldwide and represent a major focus in the field of regenerative medicine. Despite significant progress, only few stem cell platforms are currently available for cell-based therapy, disease modeling, and drug screening in the context of pul...

    journal_title:Stem cells and development

    pub_type: 杂志文章

    doi:10.1089/scd.2014.0512

    authors: Hannan NR,Sampaziotis F,Segeritz CP,Hanley NA,Vallier L

    更新日期:2015-07-15 00:00:00

  • Secretory profiles and wound healing effects of human amniotic fluid-derived mesenchymal stem cells.

    abstract::Recent evidence shows that amniotic fluid (AF) contains multiple cell types derived from the developing fetus, and may represent a novel source of stem cells for cell therapy. In this study, we examined the paracrine factors released by human amniotic fluid-derived mesenchymal stem cells (AF-MSCs) and their ability to...

    journal_title:Stem cells and development

    pub_type: 杂志文章

    doi:10.1089/scd.2009.0138

    authors: Yoon BS,Moon JH,Jun EK,Kim J,Maeng I,Kim JS,Lee JH,Baik CS,Kim A,Cho KS,Lee JH,Lee HH,Whang KY,You S

    更新日期:2010-06-01 00:00:00

  • Totipotency: what it is and what it is not.

    abstract::There is surprising confusion surrounding the concept of biological totipotency, both within the scientific community and in society at large. Increasingly, ethical objections to scientific research have both practical and political implications. Ethical controversy surrounding an area of research can have a chilling ...

    journal_title:Stem cells and development

    pub_type: 杂志文章

    doi:10.1089/scd.2013.0364

    authors: Condic ML

    更新日期:2014-04-15 00:00:00

  • Nonstimulated human uncommitted mesenchymal stem cells express cell markers of mesenchymal and neural lineages.

    abstract::Ex vivo cultures of human bone marrow-derived mesenchymal stem cells (MSCs) contain subsets of progenitors exhibiting dissimilar properties. One of these subsets comprises uncommitted progenitors displaying distinctive features, such as morphology, a quiescent condition, growth factor production, and restricted tissue...

    journal_title:Stem cells and development

    pub_type: 杂志文章

    doi:10.1089/scd.2005.14.408

    authors: Minguell JJ,Fierro FA,Epuñan MJ,Erices AA,Sierralta WD

    更新日期:2005-08-01 00:00:00

  • Fibroblastic potential of CD41+ cells in the mouse aorta-gonad-mesonephros region and yolk sac.

    abstract::CD41 (αIIb integrin), a specific marker for megakaryocytes and platelets, was recently shown to mark the initiation of definitive hematopoiesis in mouse embryos. However, whether embryonic CD41(+) populations have a nonhematopoietic potential remains elusive. Here, we report that the CD41(+) cells from the mouse E11.0...

    journal_title:Stem cells and development

    pub_type: 杂志文章

    doi:10.1089/scd.2011.0572

    authors: Zhou J,Chen H,Li S,Xie Y,He W,Nan X,Yue W,Liu B,Pei X

    更新日期:2012-09-20 00:00:00

  • Status and potential commercial impact of stem cell-based treatments on dental and craniofacial regeneration.

    abstract::The projected annual demand for stem cell-based dental treatments in the United States can range from over 290 million tooth restorative treatments to 30,000 patients requiring tissue regeneration following craniofacial cancer surgery. Professional dental treatments cost Americans over $60 billion per year. Scientific...

    journal_title:Stem cells and development

    pub_type: 杂志文章,评审

    doi:10.1089/scd.2006.15.881

    authors: Garcia-Godoy F,Murray PE

    更新日期:2006-12-01 00:00:00

  • Extracellular Vesicles Suppress Basal and Lipopolysaccharide-Induced NFκB Activity in Human Periodontal Ligament Stem Cells.

    abstract::Periodontitis is an infectious disease characterized by chronic inflammation and progressive destruction of periodontal tissues. Chronic inflammatory environment may affect immunomodulatory function of periodontal ligament stem cells (PDLSCs) and promote shift toward proinflammatory phenotype contributing to propagati...

    journal_title:Stem cells and development

    pub_type: 杂志文章

    doi:10.1089/scd.2019.0021

    authors: Čebatariūnienė A,Kriaučiūnaitė K,Prunskaitė J,Tunaitis V,Pivoriūnas A

    更新日期:2019-08-01 00:00:00

  • Identification and potential application of human corneal endothelial progenitor cells.

    abstract::The corneal endothelium is believed to be developmentally originated from the periocular mesenchyme via the neural crest. Human corneal endothelial progenitor cells (HCEPs) have been investigated because of their potential availability for the tissue regenerative medicine. However, the existence and the properties of ...

    journal_title:Stem cells and development

    pub_type: 杂志文章

    doi:10.1089/scd.2013.0387

    authors: Hara S,Hayashi R,Soma T,Kageyama T,Duncan T,Tsujikawa M,Nishida K

    更新日期:2014-09-15 00:00:00

  • Comparisons between transcriptional regulation and RNA expression in human embryonic stem cell lines.

    abstract::Recent studies have focused on transcriptional regulation and gene expression profiling of human embryonic stem cells (hESCs). However, little information is available regarding the relationship between RNA expression and transcriptional regulation, which is critical in the complete understanding of pluripotency and d...

    journal_title:Stem cells and development

    pub_type: 杂志文章

    doi:10.1089/scd.2006.15.315

    authors: Player A,Wang Y,Bhattacharya B,Rao M,Puri RK,Kawasaki ES

    更新日期:2006-06-01 00:00:00

  • Periostin secreted by mesenchymal stem cells supports tendon formation in an ectopic mouse model.

    abstract::True tendon regeneration in human patients remains a vision of musculoskeletal therapies. In comparison to other mesenchymal lineages the biology of tenogenic differentiation is barely understood. Specifically, easy and efficient protocols are lacking that might enable tendon cell and tissue differentiation based on a...

    journal_title:Stem cells and development

    pub_type: 杂志文章

    doi:10.1089/scd.2014.0124

    authors: Noack S,Seiffart V,Willbold E,Laggies S,Winkel A,Shahab-Osterloh S,Flörkemeier T,Hertwig F,Steinhoff C,Nuber UA,Gross G,Hoffmann A

    更新日期:2014-08-15 00:00:00

  • Glutamate Increases In Vitro Survival and Proliferation and Attenuates Oxidative Stress-Induced Cell Death in Adult Spinal Cord-Derived Neural Stem/Progenitor Cells via Non-NMDA Ionotropic Glutamate Receptors.

    abstract::Traumatic spinal cord injury (SCI) leads to a cascade of secondary chemical insults, including oxidative stress and glutamate excitotoxicity, which damage host neurons and glia. Transplantation of exogenous neural stem/progenitor cells (NSPCs) has shown promise in enhancing regeneration after SCI, although survival of...

    journal_title:Stem cells and development

    pub_type: 杂志文章

    doi:10.1089/scd.2015.0389

    authors: Hachem LD,Mothe AJ,Tator CH

    更新日期:2016-08-15 00:00:00

  • A view of bivalent epigenetic marks in two human embryonic stem cell lines reveals a different cardiogenic potential.

    abstract::Human embryonic stem (HUES) cells are derived from early individual embryos with unique genetic printing. However, how their epigenetic status might affect their potential to differentiate toward specific lineages remains a puzzling question. Using chromatin immunoprecipitation (ChIP)-polymerase chain reaction and ChI...

    journal_title:Stem cells and development

    pub_type: 杂志文章

    doi:10.1089/scd.2014.0345

    authors: Leschik J,Caron L,Yang H,Cowan C,Pucéat M

    更新日期:2015-02-01 00:00:00

  • Pax6 is essential for the maintenance and multi-lineage differentiation of neural stem cells, and for neuronal incorporation into the adult olfactory bulb.

    abstract::The paired type homeobox 6 (Pax6) transcription factor (TF) regulates multiple aspects of neural stem cell (NSC) and neuron development in the embryonic central nervous system. However, less is known about the role of Pax6 in the maintenance and differentiation of adult NSCs and in adult neurogenesis. Using the +/Sey(...

    journal_title:Stem cells and development

    pub_type: 杂志文章

    doi:10.1089/scd.2014.0058

    authors: Curto GG,Nieto-Estévez V,Hurtado-Chong A,Valero J,Gómez C,Alonso JR,Weruaga E,Vicario-Abejón C

    更新日期:2014-12-01 00:00:00

  • Transfer of growth factor receptor mRNA via exosomes unravels the regenerative effect of mesenchymal stem cells.

    abstract::Bone marrow-mesenchymal stem cells (BM-MSC) ameliorate renal dysfunction and repair tubular damage of acute kidney injury by locally releasing growth factors, including the insulin-like growth factor-1 (IGF-1). The restricted homing of BM-MSC at the site of injury led us to investigate a possible gene-based communicat...

    journal_title:Stem cells and development

    pub_type: 杂志文章

    doi:10.1089/scd.2012.0266

    authors: Tomasoni S,Longaretti L,Rota C,Morigi M,Conti S,Gotti E,Capelli C,Introna M,Remuzzi G,Benigni A

    更新日期:2013-03-01 00:00:00

  • Role of environmental chemicals, processed food derivatives, and nutrients in the induction of carcinogenesis.

    abstract::In recent years it has been hypothesized that cancer stem cells (CSCs) are the actual driving force of tumor formation, highlighting the need to specifically target CSCs to successfully eradicate cancer growth and recurrence. Particularly, the deregulation of physiological signaling pathways controlling stem cell prol...

    journal_title:Stem cells and development

    pub_type: 杂志文章,评审

    doi:10.1089/scd.2015.0081

    authors: Persano L,Zagoura D,Louisse J,Pistollato F

    更新日期:2015-10-15 00:00:00

  • Adipose-derived stromal cells inhibit allergic airway inflammation in mice.

    abstract::Allergic asthma is an inflammatory airway disease caused by T helper type 2 (Th2)-driven immune responses. Recent studies have demonstrated that adipose-derived stromal cells (ASC) have an immunosuppressive effect on T-cell activity. This study was performed to investigate whether ASC can inhibit Th2-dependent allergi...

    journal_title:Stem cells and development

    pub_type: 杂志文章

    doi:10.1089/scd.2009.0513

    authors: Park HK,Cho KS,Park HY,Shin DH,Kim YK,Jung JS,Park SK,Roh HJ

    更新日期:2010-11-01 00:00:00

  • Human amniotic fluid-derived mesenchymal cells from fetuses with a neural tube defect do not deposit collagen type i protein after TGF-β1 stimulation in vitro.

    abstract::In spina bifida, the neural tube fails to close during the embryonic period. Exposure of the neural tube to the amniotic fluid during pregnancy causes additional neural damage. Intrauterine tissue engineering using a biomaterial seeded with stem cells might prevent this additional damage. For this purpose, autologous ...

    journal_title:Stem cells and development

    pub_type: 杂志文章

    doi:10.1089/scd.2013.0334

    authors: Hosper NA,Bank RA,van den Berg PP

    更新日期:2014-03-01 00:00:00

  • Forced Runx1 expression in human neural stem/progenitor cells transplanted to the rat dorsal root ganglion cavity results in extensive axonal growth specifically from spinal cord-derived neurospheres.

    abstract::Cell replacement therapy holds great promise for treating a wide range of human disorders. However, ensuring the predictable differentiation of transplanted stem cells, eliminating their risk of tumor formation, and generating fully functional cells after transplantation remain major challenges in regenerative medicin...

    journal_title:Stem cells and development

    pub_type: 杂志文章

    doi:10.1089/scd.2010.0555

    authors: König N,Åkesson E,Telorack M,Vasylovska S,Ngamjariyawat A,Sundström E,Oster A,Trolle C,Berens C,Aldskogius H,Seiger Å,Kozlova EN

    更新日期:2011-11-01 00:00:00

  • Scaffold-in-Scaffold Potential to Induce Growth and Differentiation of Cardiac Progenitor Cells.

    abstract::The design of reliable biocompatible and biodegradable scaffolds remains one of the most important challenges for tissue engineering. In fact, properly designed scaffolds must display an adequate and interconnected porosity to facilitate cell spreading and colonization of the inner layers, and must release physical si...

    journal_title:Stem cells and development

    pub_type: 杂志文章

    doi:10.1089/scd.2017.0051

    authors: Ciocci M,Mochi F,Carotenuto F,Di Giovanni E,Prosposito P,Francini R,De Matteis F,Reshetov I,Casalboni M,Melino S,Di Nardo P

    更新日期:2017-10-01 00:00:00

  • Role of Epigenetic Regulation by the REST/CoREST/HDAC Corepressor Complex of Moderate NANOG Expression in Chicken Primordial Germ Cells.

    abstract::Primordial germ cells (PGCs), the precursors of gametes, have regulatory mechanisms involving transcription factors and epigenetic modifications. The transcription factor NANOG is a key regulator of germ cell and embryonic stem cell characteristics. However, the epigenetic regulation of NANOG with a histone deacetylas...

    journal_title:Stem cells and development

    pub_type: 杂志文章

    doi:10.1089/scd.2018.0059

    authors: Jung HG,Hwang YS,Park YH,Cho HY,Rengaraj D,Han JY

    更新日期:2018-09-01 00:00:00

  • Oleate ameliorates palmitate-induced impairment of differentiative capacity in C2C12 myoblast cells.

    abstract::A common observation in metabolic disorders and aging is the elevation of free fatty acids, which can form ectopic fat deposition and result in lipotoxicity. Ectopic fat deposition of skeletal muscle has been recognized as an important component of aging, frailty and sarcopenia. Previous studies have suggested that li...

    journal_title:Stem cells and development

    pub_type: 杂志文章

    doi:10.1089/scd.2020.0168

    authors: Xue M,Zhang F,Ji X,Yu H,Jiang X,Qiu Y,Yu J,Chen J,Yang F,Bao Z

    更新日期:2021-01-11 00:00:00

  • Human stem/progenitor cells from bone marrow enhance glial differentiation of rat neural stem cells: a role for transforming growth factor β and Notch signaling.

    abstract::Multipotent stem/progenitor cells from bone marrow stroma (mesenchymal stromal cells or MSCs) were previously shown to enhance proliferation and differentiation of neural stem cells (NSCs) in vivo, but the molecular basis of the effect was not defined. Here coculturing human MSCs (hMSCs) with rat NSCs (rNSCs) was foun...

    journal_title:Stem cells and development

    pub_type: 杂志文章

    doi:10.1089/scd.2009.0444

    authors: Robinson AP,Foraker JE,Ylostalo J,Prockop DJ

    更新日期:2011-02-01 00:00:00

  • The mental retardation-associated protein srGAP3 regulates survival, proliferation, and differentiation of rat embryonic neural stem/progenitor cells.

    abstract::The mental retardation-associated protein, srGAP3 is highly expressed in neurogenic sites. It is thought to regulate the key aspects of neuronal development and functions. Little is known about the interaction between srGAP3 and immature neural stem cells/neural progenitor cells (NSCs/NPCs). In the current study, the ...

    journal_title:Stem cells and development

    pub_type: 杂志文章

    doi:10.1089/scd.2012.0455

    authors: Lu H,Jiao Q,Wang Y,Yang Z,Feng M,Wang L,Chen X,Jin W,Liu Y

    更新日期:2013-06-01 00:00:00

  • Identification of Ccr4-not complex components as regulators of transition from partial to genuine induced pluripotent stem cells.

    abstract::Somatic cells can be reprogrammed to induced pluripotent stem cells (iPSCs) by defined factors. However, substantial cell numbers subjected to iPSC induction stray from the main reprogramming route and are immortalized as partial iPSCs. These partial iPSCs can become genuine iPSCs by exposure to the ground state condi...

    journal_title:Stem cells and development

    pub_type: 杂志文章

    doi:10.1089/scd.2013.0326

    authors: Kamon M,Katano M,Hiraki-Kamon K,Hishida T,Nakachi Y,Mizuno Y,Okazaki Y,Suzuki A,Hirasaki M,Ueda A,Nishimoto M,Kato H,Okuda A

    更新日期:2014-09-15 00:00:00

  • Exon skipping and gene transfer restore dystrophin expression in human induced pluripotent stem cells-cardiomyocytes harboring DMD mutations.

    abstract::With an incidence of ∼1:3,500 to 5,000 in male children, Duchenne muscular dystrophy (DMD) is an X-linked disorder in which progressive muscle degeneration occurs and affected boys usually die in their twenties or thirties. Cardiac involvement occurs in 90% of patients and heart failure accounts for up to 40% of death...

    journal_title:Stem cells and development

    pub_type: 杂志文章

    doi:10.1089/scd.2013.0135

    authors: Dick E,Kalra S,Anderson D,George V,Ritso M,Laval SH,Barresi R,Aartsma-Rus A,Lochmüller H,Denning C

    更新日期:2013-10-15 00:00:00

  • Human Wharton's Jelly-Derived Stem Cells Display a Distinct Immunomodulatory and Proregenerative Transcriptional Signature Compared to Bone Marrow-Derived Stem Cells.

    abstract::Mesenchymal stromal cells (MSCs) are multipotent stem cells with immunosuppressive and trophic support functions. While MSCs from different sources frequently display a similar appearance in culture, they often show differences in their surface marker and gene expression profiles. Although bone marrow is considered th...

    journal_title:Stem cells and development

    pub_type: 杂志文章

    doi:10.1089/scd.2017.0029

    authors: Donders R,Bogie JFJ,Ravanidis S,Gervois P,Vanheusden M,Marée R,Schrynemackers M,Smeets HJM,Pinxteren J,Gijbels K,Walbers S,Mays RW,Deans R,Van Den Bosch L,Stinissen P,Lambrichts I,Gyselaers W,Hellings N

    更新日期:2018-01-15 00:00:00

  • Engineered Mesenchymal Stem Cells as an Anti-Cancer Trojan Horse.

    abstract::Cell-based gene therapy holds a great promise for the treatment of human malignancy. Among different cells, mesenchymal stem cells (MSCs) are emerging as valuable anti-cancer agents that have the potential to be used to treat a number of different cancer types. They have inherent migratory properties, which allow them...

    journal_title:Stem cells and development

    pub_type: 杂志文章

    doi:10.1089/scd.2016.0120

    authors: Nowakowski A,Drela K,Rozycka J,Janowski M,Lukomska B

    更新日期:2016-10-01 00:00:00

  • Short-term culture of umbilical cord blood-derived CD34 cells enhances engraftment into NOD/SCID mice through increased CXCR4 expression.

    abstract::Human umbilical cord blood (CB) has been used successfully in stem cell transplantation. A subpopulation of CD34(+) cells expresses chemokine receptor CXCR4 which is critical for bone marrow engraftment in human hematopoietic stem cells. Here, we demonstrate the effect of short-term culture on CXCR4 expression on umbi...

    journal_title:Stem cells and development

    pub_type: 杂志文章

    doi:10.1089/scd.2008.0298

    authors: Ohno N,Kajiume T,Sera Y,Sato T,Kobayashi M

    更新日期:2009-10-01 00:00:00