Generation of Canine Induced Extraembryonic Endoderm-Like Cell Line That Forms Both Extraembryonic and Embryonic Endoderm Derivatives.


:Extraembryonic endoderm (XEN) cells are stem cell lines derived from primitive endoderm cells of inner cell mass in blastocysts. These cells have self-renewal properties and differentiate into visceral endoderm (VE) and parietal endoderm (PE) of the yolk sac. Recently, it has been reported that XEN cells can contribute to fetal embryonic endoderm, and their unique potency has been evaluated. In this study, we have described the induction and characterization of new canine stem cell lines that closely resemble to XEN cells. These cells, which we designated canine induced XEN (ciXEN)-like cells, were induced from canine embryonic fibroblasts by introducing four transgenes. ciXEN-like cells expressed XEN markers, which could be maintained over 50 passages in N2B27 medium supplemented with inhibitors of mitogen-activated protein kinase p38 and transforming growth factor-beta 1. Our ciXEN-like cells were maintained without transgene expression and exhibited upregulated expression of VE and PE markers in feeder-free conditions. The cells differentiated from ciXEN-like cells using a coculture system showed multiple nuclei and expressed albumin protein, similar to characteristics of hepatocytes. Furthermore, these cells expressed the adult hepatocyte marker, CYP3A4. Interestingly, these cells also formed a net structure expressing the bile epithelium capillary marker, multidrug resistance-associated protein 2. Thus, we have demonstrated the induction of a new canine stem cell line, ciXEN-like cells, which could form an embryonic endodermal cell layer. Our ciXEN-like cells may be a helpful tool to study the canine embryo development and represent a promising cell source for proceeding human and canine regenerative medicine.


Stem Cells Dev


Nishimura T,Unezaki N,Kanegi R,Wijesekera DPH,Hatoya S,Sugiura K,Kawate N,Tamada H,Imai H,Inaba T




Has Abstract


2017-08-01 00:00:00












  • Genetic Mouse Models and Induced Pluripotent Stem Cells for Studying Tracheal-Esophageal Separation and Esophageal Development.

    abstract::Esophagus and trachea arise from a common origin, the anterior foregut tube. The compartmentalization process of the foregut into the esophagus and trachea is still poorly understood. Esophageal atresia/tracheoesophageal fistula (EA/TEF) is one of the most common gastrointestinal congenital defects with an incidence r...

    journal_title:Stem cells and development

    pub_type: 杂志文章


    authors: Raad S,David A,Que J,Faure C

    更新日期:2020-08-01 00:00:00

  • Therapeutic Prospect of Adipose-Derived Stromal Cells for the Treatment of Abdominal Aortic Aneurysm.

    abstract::Aneurysm refers to the dilation of the vessel wall for more than 50%. Abdominal aortic aneurysm (AAA) refers to the dilation and weakening of all three layers of the abdominal aorta, which mostly occur infrarenally. The population aged above 50 years is at risk of AAA development, while a familiar history doubles the ...

    journal_title:Stem cells and development

    pub_type: 杂志文章,评审


    authors: Parvizi M,Harmsen MC

    更新日期:2015-07-01 00:00:00

  • Long-term serial passage and neuronal differentiation capability of human bone marrow mesenchymal stem cells.

    abstract::The development of methods to induce differentiation of human mesenchymal stem cells (hMSCs) has opened the possibility of using these cells in regenerative or reparative therapies. However, the low frequency of hMSCs in tissue means it is often necessary to expand these cells extensively in vitro. In this study, we e...

    journal_title:Stem cells and development

    pub_type: 杂志文章


    authors: Khoo ML,Shen B,Tao H,Ma DD

    更新日期:2008-10-01 00:00:00

  • LPS-stimulated inflammatory environment inhibits BMP-2-induced osteoblastic differentiation through crosstalk between TLR4/MyD88/NF-κB and BMP/Smad signaling.

    abstract::Bone morphogenetic protein-2 (BMP-2) is a novel differentiation factor that is capable of inducing osteoblast differentiation and bone formation, making it an attractive option in treatment of bone defects, fractures, and spine fusions. Inflammation, which was a common situation during bone healing, is recognized to i...

    journal_title:Stem cells and development

    pub_type: 杂志文章


    authors: Huang RL,Yuan Y,Zou GM,Liu G,Tu J,Li Q

    更新日期:2014-02-01 00:00:00

  • Human amniotic fluid-derived mesenchymal cells from fetuses with a neural tube defect do not deposit collagen type i protein after TGF-β1 stimulation in vitro.

    abstract::In spina bifida, the neural tube fails to close during the embryonic period. Exposure of the neural tube to the amniotic fluid during pregnancy causes additional neural damage. Intrauterine tissue engineering using a biomaterial seeded with stem cells might prevent this additional damage. For this purpose, autologous ...

    journal_title:Stem cells and development

    pub_type: 杂志文章


    authors: Hosper NA,Bank RA,van den Berg PP

    更新日期:2014-03-01 00:00:00

  • An adipoinductive role of inflammation in adipose tissue engineering: key factors in the early development of engineered soft tissues.

    abstract::Tissue engineering and cell implantation therapies are gaining popularity because of their potential to repair and regenerate tissues and organs. To investigate the role of inflammatory cytokines in new tissue development in engineered tissues, we have characterized the nature and timing of cell populations forming ne...

    journal_title:Stem cells and development

    pub_type: 杂志文章


    authors: Lilja HE,Morrison WA,Han XL,Palmer J,Taylor C,Tee R,Möller A,Thompson EW,Abberton KM

    更新日期:2013-05-15 00:00:00

  • Periodontal Ligament Stem Cells: Regenerative Potency in Periodontium.

    abstract::Periodontium is consisted of root cementum, bone lining the tooth socket, gingiva facing the tooth, and periodontal ligament (PDL). Its primary functions are support of the tooth and protection of tooth, nerve, and blood vessels from injury by mechanical loading. Severe periodontitis induces the destruction of periodo...

    journal_title:Stem cells and development

    pub_type: 杂志文章,评审


    authors: Tomokiyo A,Wada N,Maeda H

    更新日期:2019-08-01 00:00:00

  • Mesenchymal Stem Cell-Derived Exosomes: Hope for Spinal Cord Injury Repair.

    abstract::Spinal cord injury (SCI) is a devastating medical condition with profound social and economic impacts. Although research is ongoing, current treatment options are limited and do little to restore functionality. However, recent studies suggest that mesenchymal stem cell-derived exosomes (MSC-exosomes) may hold the key ...

    journal_title:Stem cells and development

    pub_type: 杂志文章


    authors: Ren Z,Qi Y,Sun S,Tao Y,Shi R

    更新日期:2020-12-01 00:00:00

  • Novel culture system of mesenchymal stromal cells from human subcutaneous adipose tissue.

    abstract::Accumulating evidence suggests that the delivery of human adipose tissue-derived stromal cells (hASCs) has great potential as regenerative therapy. This was performed to develop a method for expanding hASCs by reducing the amount of serum required. We demonstrate that hASCs were able to expand efficiently in media con...

    journal_title:Stem cells and development

    pub_type: 杂志文章


    authors: Iwashima S,Ozaki T,Maruyama S,Saka Y,Kobori M,Omae K,Yamaguchi H,Niimi T,Toriyama K,Kamei Y,Torii S,Murohara T,Yuzawa Y,Kitagawa Y,Matsuo S

    更新日期:2009-05-01 00:00:00

  • Conversion of human bone marrow-derived mesenchymal stem cells into tendon progenitor cells by ectopic expression of scleraxis.

    abstract::Tendons and ligaments (T/L) are dense connective tissues of mesodermal origin. During embryonic development, the tendon-specific cells descend from a sub-set of mesenchymal progenitors condensed in the syndetome, a dorsolateral domain of the sclerotome. These cells are defined by the expression of the transcription fa...

    journal_title:Stem cells and development

    pub_type: 杂志文章


    authors: Alberton P,Popov C,Prägert M,Kohler J,Shukunami C,Schieker M,Docheva D

    更新日期:2012-04-10 00:00:00

  • Teratogenic potential in cultures optimized for oligodendrocyte development from mouse embryonic stem cells.

    abstract::We describe a rapid and efficient 5-step program of defined factors for the genesis of brain myelin-forming oligodendrocytes (OLs) from embryonic stem cells (ESCs). The OLs emerge on the same time frame in vitro as seen in vivo. Factors promoting neural induction (retinoids, noggin) are required, while exogenous Sonic...

    journal_title:Stem cells and development

    pub_type: 杂志文章


    authors: Sadowski D,Kiel ME,Apicella M,Arriola AG,Chen CP,McKinnon RD

    更新日期:2010-09-01 00:00:00

  • Human umbilical cord blood-derived mesenchymal stem cells undergo cellular senescence in response to oxidative stress.

    abstract::Since human mesenchymal stem cells (MSCs) are therapeutically attractive for tissue regeneration and repair, we examined the physiological responses of human umbilical cord blood-derived MSCs (hUCB-MSCs) to genotoxic stress. We found that that sublethal doses of reactive oxygen species (ROS) and ionizing radiation cau...

    journal_title:Stem cells and development

    pub_type: 杂志文章


    authors: Ko E,Lee KY,Hwang DS

    更新日期:2012-07-20 00:00:00

  • Biodistribution of Glial Progenitors in a Three Dimensional-Printed Model of the Piglet Cerebral Ventricular System.

    abstract::White matter damage persists in hypoxic-ischemic newborns even when treated with hypothermia. We have previously shown that intraventricular delivery of human glial progenitors (GPs) at the neonatal stage is capable of replacing abnormal host glia and rescuing the lifespan of dysmyelinated mice. However, such transpla...

    journal_title:Stem cells and development

    pub_type: 杂志文章


    authors: Srivastava RK,Jablonska A,Chu C,Gregg L,Bulte JWM,Koehler RC,Walczak P,Janowski M

    更新日期:2019-04-15 00:00:00

  • Telomerase Reverse Transcriptase Expression in Mouse Endometrium During Reepithelialization and Regeneration in a Menses-Like Model.

    abstract::The regenerative capacity of the endometrium has been attributed to resident stem/progenitor cells. A number of stem/progenitor markers have been reported for human endometrial stem/progenitor cells; however, the lack of convenient markers in the mouse has made experimental investigation into endometrial regeneration ...

    journal_title:Stem cells and development

    pub_type: 杂志文章


    authors: Cousins FL,O DF,Ong YR,Breault DT,Deane JA,Gargett CE

    更新日期:2019-01-01 00:00:00

  • Extracellular Vesicles Suppress Basal and Lipopolysaccharide-Induced NFκB Activity in Human Periodontal Ligament Stem Cells.

    abstract::Periodontitis is an infectious disease characterized by chronic inflammation and progressive destruction of periodontal tissues. Chronic inflammatory environment may affect immunomodulatory function of periodontal ligament stem cells (PDLSCs) and promote shift toward proinflammatory phenotype contributing to propagati...

    journal_title:Stem cells and development

    pub_type: 杂志文章


    authors: Čebatariūnienė A,Kriaučiūnaitė K,Prunskaitė J,Tunaitis V,Pivoriūnas A

    更新日期:2019-08-01 00:00:00

  • Depsipeptide (FK228) preferentially induces apoptosis in BCR/ABL-expressing cell lines and cells from patients with chronic myelogenous leukemia in blast crisis.

    abstract::Resistance to imatinib can occur in patients with chronic myelogenous leukemia (CML). In this study, we report mechanisms of action of histone deacetylase (HDAC) inhibitor, depsipeptide (FK228) in BCR/ABL-expressing cell lines and its effectiveness in imatinib-resistant cells from patients with blast crisis of CML. FK...

    journal_title:Stem cells and development

    pub_type: 杂志文章


    authors: Okabe S,Tauchi T,Nakajima A,Sashida G,Gotoh A,Broxmeyer HE,Ohyashiki JH,Ohyashiki K

    更新日期:2007-06-01 00:00:00

  • Role of Fibroblast Growth Factor-5 on the Proliferation of Human Tonsil-Derived Mesenchymal Stem Cells.

    abstract::Human mesenchymal stem cells (MSCs) are a promising tool for therapeutic applications in cell-based therapy and regenerative medicine, and MSCs from the human palatine tonsils have recently been used as a new tissue source. However, the understanding of the proliferation and differentiation capacity of tonsil-derived ...

    journal_title:Stem cells and development

    pub_type: 杂志文章


    authors: Park GC,Song JS,Park HY,Shin SC,Jang JY,Lee JC,Wang SG,Lee BJ,Jung JS

    更新日期:2016-08-01 00:00:00

  • Induction and detection of human mesenchymal stem cell migration in the 48-well reusable transwell assay.

    abstract::The induction and detection of chemotactic migration in mesenchymal stem cells (MSC) are complicated by their adherent nature. We describe here the experimental details for inducing the transmigration of MSC seeded in microchemotaxis chambers and reliably identifying the translocated cells. A combination of 8-microm p...

    journal_title:Stem cells and development

    pub_type: 杂志文章


    authors: Rüster B,Grace B,Seitz O,Seifried E,Henschler R

    更新日期:2005-04-01 00:00:00

  • Oleate ameliorates palmitate-induced impairment of differentiative capacity in C2C12 myoblast cells.

    abstract::A common observation in metabolic disorders and aging is the elevation of free fatty acids, which can form ectopic fat deposition and result in lipotoxicity. Ectopic fat deposition of skeletal muscle has been recognized as an important component of aging, frailty and sarcopenia. Previous studies have suggested that li...

    journal_title:Stem cells and development

    pub_type: 杂志文章


    authors: Xue M,Zhang F,Ji X,Yu H,Jiang X,Qiu Y,Yu J,Chen J,Yang F,Bao Z

    更新日期:2021-01-11 00:00:00

  • Short-term culture of umbilical cord blood-derived CD34 cells enhances engraftment into NOD/SCID mice through increased CXCR4 expression.

    abstract::Human umbilical cord blood (CB) has been used successfully in stem cell transplantation. A subpopulation of CD34(+) cells expresses chemokine receptor CXCR4 which is critical for bone marrow engraftment in human hematopoietic stem cells. Here, we demonstrate the effect of short-term culture on CXCR4 expression on umbi...

    journal_title:Stem cells and development

    pub_type: 杂志文章


    authors: Ohno N,Kajiume T,Sera Y,Sato T,Kobayashi M

    更新日期:2009-10-01 00:00:00

  • Cell-based therapy for therapeutic lymphangiogenesis.

    abstract::Lymphedema is a medically irreversible condition for which currently conservative and surgical therapies are either ineffective or impractical. The potential use of progenitor and stem cell-based therapies has offered a paradigm that may provide alternative treatment options for lymphatic disorders. Moreover, basic re...

    journal_title:Stem cells and development

    pub_type: 杂志文章,评审


    authors: Qi S,Pan J

    更新日期:2015-02-01 00:00:00

  • Haploinsufficiency of senescence evasion factor causes defects of hematopoietic stem cells functions.

    abstract::The quality of hematopoietic stem cells (HSCs) is essentially defined by two characteristics, i.e., multilineage differentiation and self-renewal capacity. Thus, it is of high priority to clarify mechanisms that regulate these functions and to understand them at the molecular level. In the present study, we investigat...

    journal_title:Stem cells and development

    pub_type: 杂志文章


    authors: Schraml E,Voglauer R,Fortschegger K,Sibilia M,Stelzer I,Grillari J,Schauenstein K

    更新日期:2008-04-01 00:00:00

  • Evaluation of the reactivity of apoptosis markers before and after cryopreservation in cord blood CD34(+) cells.

    abstract::Umbilical cord blood (CB) CD34(+) cells, on the basis of flow cytometry analysis, are comprised of multiple populations. In in vitro assays, only CD34(regular) FSC(high) cells are functional and low percentages of nonfunctional CD34(regular) FSC(low) cells were determined to be present in liquid-stored CB. Liquid-stor...

    journal_title:Stem cells and development

    pub_type: 杂志文章


    authors: Greco NJ,Seetharaman S,Kurtz J,Lee WR,Moroff G

    更新日期:2006-02-01 00:00:00

  • CD34 modulates the trafficking behavior of hematopoietic cells in vivo.

    abstract::The CD34 surface antigen has been recognized as a marker of hematopoietic stem cells (HSCs) and is widely used for HSC selection as well as for quality control in HSC transplantation. CD34 has been implicated in cytoadhesion signaling, and its expression has been suggested to reflect the activation state of hematopoie...

    journal_title:Stem cells and development

    pub_type: 杂志文章


    authors: Lange C,Li Z,Fang L,Baum C,Fehse B

    更新日期:2007-04-01 00:00:00

  • Bone morphogenetic protein signaling is involved in human mesenchymal stem cell survival in serum-free medium.

    abstract::Bone marrow human mesenchymal stem cells (hMSCs) are known to survive in serum-free media, when most normal somatic cells do not survive. We found that the endogenously-activated bone morphogenetic protein (BMP) pathway is involved in this cellular behavior. Under this culture condition, phosphorylated Smad1 (PSmad1),...

    journal_title:Stem cells and development

    pub_type: 杂志文章


    authors: Solmesky LJ,Abekasis M,Bulvik S,Weil M

    更新日期:2009-11-01 00:00:00

  • Improved mobilization of the CD34(+) and CD133(+) bone marrow-derived circulating progenitor cells by freshly isolated intracoronary bone marrow cell transplantation in patients with ischemic heart disease.

    abstract::Cell therapy is a promising novel option for treatment of cardiovascular disease. Because the role of bone marrow-derived circulating progenitor cells (BM-CPCs) after cell therapy is less clear, we analyzed in this randomized, controlled study the influence of intracoronary autologous freshly isolated bone marrow cell...

    journal_title:Stem cells and development

    pub_type: 杂志文章,随机对照试验


    authors: Turan RG,Bozdag-Turan I,Ortak J,Akin I,Kische S,Schneider H,Turan CH,Rehders TC,Rauchhaus M,Kleinfeldt T,Adolph E,Brehm M,Yokus S,Steiner S,Sahin K,Nienaber CA,Ince H

    更新日期:2011-09-01 00:00:00

  • Role of low voltage activated calcium channels in neuritogenesis and active migration of embryonic neural progenitor cells.

    abstract::The central role of calcium influx and electrical activity in embryonic development raises important questions about the role and regulation of voltage-dependent calcium influx. Using cultured neural progenitor cell (NPC) preparations, we recorded barium currents through voltage-activated channels using the whole-cell...

    journal_title:Stem cells and development

    pub_type: 杂志文章


    authors: Louhivuori LM,Louhivuori V,Wigren HK,Hakala E,Jansson LC,Nordström T,Castrén ML,Akerman KE

    更新日期:2013-04-15 00:00:00

  • Epoxyeicosatrienoic Acids Regulate Adipocyte Differentiation of Mouse 3T3 Cells, Via PGC-1α Activation, Which Is Required for HO-1 Expression and Increased Mitochondrial Function.

    abstract::Epoxyeicosatrienoic acid (EET) contributes to browning of white adipose stem cells to ameliorate obesity/diabetes and insulin resistance. In the current study, we show that EET altered preadipocyte function, enhanced peroxisome proliferation-activated receptor γ coactivator α (PGC-1α) expression, and increased mitocho...

    journal_title:Stem cells and development

    pub_type: 杂志文章


    authors: Waldman M,Bellner L,Vanella L,Schragenheim J,Sodhi K,Singh SP,Lin D,Lakhkar A,Li J,Hochhauser E,Arad M,Darzynkiewicz Z,Kappas A,Abraham NG

    更新日期:2016-07-15 00:00:00

  • Metformin Preconditioning of Human Induced Pluripotent Stem Cell-Derived Neural Stem Cells Promotes Their Engraftment and Improves Post-Stroke Regeneration and Recovery.

    abstract::While transplantation of human induced pluripotent stem cell-derived neural stem cells (hiPSC-NSCs) shows therapeutic potential in animal stroke models, major concerns for translating hiPSC therapy to the clinic are efficacy and safety. Therefore, there is a demand to develop an optimal strategy to enhance the engraft...

    journal_title:Stem cells and development

    pub_type: 杂志文章


    authors: Ould-Brahim F,Sarma SN,Syal C,Lu KJ,Seegobin M,Carter A,Jeffers MS,Doré C,Stanford WL,Corbett D,Wang J

    更新日期:2018-08-15 00:00:00

  • AMPK Knockdown in Placental Labyrinthine Progenitor Cells Results in Restriction of Critical Energy Resources and Terminal Differentiation Failure.

    abstract::Placental abnormalities can cause Pregnancy-Associated Disorders, including preeclampsia, intrauterine growth restriction, and placental insufficiency, resulting in complications for both the mother and fetus. Trophoblast cells within the labyrinthine layer of the placenta facilitate the exchange of nutrients, gases, ...

    journal_title:Stem cells and development

    pub_type: 杂志文章


    authors: Waker CA,Albers RE,Pye RL,Doliboa SR,Wyatt CN,Brown TL,Mayes DA

    更新日期:2017-06-01 00:00:00