Abstract：
:We have isolated a novel progenitor cell population from adult rat pancreatic ducts, termed pancreatic-derived progenitor cells (PDPCs). Here, we report the in vitro culture, selection, and characterization of Thy1.1-positive and Thy1.1-negative PDPC subpopulations. These cells exhibit bipotentiality for differentiation into both pancreatic and hepatic cell types. Significantly, they express Pdx-1. Using a serum-free FGF-4-containing differentiation protocol, we have observed a time course of both morphological and gene expression changes indicative of hepatic lineage differentiation for the Thy1.1-positive subpopulation. These cells express albumin and store glycogen, typical features of mature hepatocytes. The Thy1.1-positive subpopulation could also readily be induced to differentiate into a pancreatic lineage with characteristic morphological changes resulting in three-dimensional islet-like structures and the transcriptional expression of insulin and glucagon in addition to Pdx-1. No morphological evidence of islet-like clusters was observed using the Thy1.1-negative population. However, Thy1.1-negative cells grown in pancreatic differentiation medium did show insulin gene transcription. Glucagon was not expressed in the undifferentiated Thy1.1-negative cells, nor was it induced in vitro after differentiation. The detection of Pdx-1 transcriptional expression in both populations indicates their potential as a novel source of non-beta-cell-derived insulin.
journal_name
Stem Cells Devjournal_title
Stem cells and developmentauthors
Stevenson KS,McGlynn L,Hodge M,McLinden H,George WD,Davies RW,Shiels PGdoi
10.1089/scd.2008.0301subject
Has Abstractpub_date
2009-12-01 00:00:00pages
1389-98issue
10eissn
1547-3287issn
1557-8534journal_volume
18pub_type
杂志文章abstract：:Administration of human bone marrow-derived mesenchymal stem cells (BM-MSCs) significantly alleviates allergic airway inflammation. There are no studies that refer to the role of microRNAs (miRNAs) after the BM-MSCs treatment in airway allergic inflammation. We induced a mouse model of asthma and performed the transpl...
journal_title：Stem cells and development
pub_type： 杂志文章
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journal_title：Stem cells and development
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doi：10.1089/scd.2009.0186
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abstract：:Human umbilical cord blood (CB) has been used successfully in stem cell transplantation. A subpopulation of CD34(+) cells expresses chemokine receptor CXCR4 which is critical for bone marrow engraftment in human hematopoietic stem cells. Here, we demonstrate the effect of short-term culture on CXCR4 expression on umbi...
journal_title：Stem cells and development
pub_type： 杂志文章
doi：10.1089/scd.2008.0298
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journal_title：Stem cells and development
pub_type： 杂志文章
doi：10.1089/scd.2018.0059
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journal_title：Stem cells and development
pub_type： 杂志文章
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journal_title：Stem cells and development
pub_type： 杂志文章
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更新日期：2013-03-15 00:00:00
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journal_title：Stem cells and development
pub_type： 杂志文章
doi：10.1089/scd.2013.0132
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journal_title：Stem cells and development
pub_type： 杂志文章
doi：10.1089/scd.2018.0157
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journal_title：Stem cells and development
pub_type： 杂志文章
doi：10.1089/scd.2013.0364
更新日期：2014-04-15 00:00:00
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journal_title：Stem cells and development
pub_type： 杂志文章
doi：10.1089/scd.2019.0145
更新日期：2020-06-01 00:00:00
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journal_title：Stem cells and development
pub_type： 杂志文章
doi：10.1089/scd.2011.0297
更新日期：2012-07-01 00:00:00
abstract：:The capability to form all cell types of the body is a unique feature of stem cells. However, many questions remain concerning the mechanisms regulating differentiation potential. The derivation of spermatogonial cell lines (SGs) from mouse and human, which can differentiate across germ-layer borders, suggested male g...
journal_title：Stem cells and development
pub_type： 杂志文章
doi：10.1089/scd.2010.0290
更新日期：2011-08-01 00:00:00
abstract：:Bone marrow human mesenchymal stem cells (hMSCs) are known to survive in serum-free media, when most normal somatic cells do not survive. We found that the endogenously-activated bone morphogenetic protein (BMP) pathway is involved in this cellular behavior. Under this culture condition, phosphorylated Smad1 (PSmad1),...
journal_title：Stem cells and development
pub_type： 杂志文章
doi：10.1089/scd.2009.0020
更新日期：2009-11-01 00:00:00
abstract：:The regeneration of lost periodontal ligament (PDL) and alveolar bone is the purpose of periodontal tissue engineering. The goal of the present study was to assess the suitability of 3 odontogenic progenitor populations from dental pulp, PDL, and dental follicle for periodontal regeneration when exposed to natural and...
journal_title：Stem cells and development
pub_type： 杂志文章
doi：10.1089/scd.2010.0431
更新日期：2011-10-01 00:00:00
abstract：:Since human mesenchymal stem cells (MSCs) are therapeutically attractive for tissue regeneration and repair, we examined the physiological responses of human umbilical cord blood-derived MSCs (hUCB-MSCs) to genotoxic stress. We found that that sublethal doses of reactive oxygen species (ROS) and ionizing radiation cau...
journal_title：Stem cells and development
pub_type： 杂志文章
doi：10.1089/scd.2011.0284
更新日期：2012-07-20 00:00:00
abstract：:The development of the heart is essential for embryogenesis and precedes development of other organs. However, the mechanisms involved in embryonic cardiac development are ill-defined. Recent evidence suggests that Smad and Wnt signaling pathways are important in stem cell fate determination and their commitment to ca...
journal_title：Stem cells and development
pub_type： 杂志文章
doi：10.1089/scd.2006.15.29
更新日期：2006-02-01 00:00:00
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journal_title：Stem cells and development
pub_type： 杂志文章
doi：10.1089/scd.2006.0109
更新日期：2007-06-01 00:00:00
abstract：:Inducing a stable and predictable program of neural cell fate in pluripotent cells in vitro is an important goal for utilizing these cells for modeling human disease mechanisms. However, the extent to which in vitro neural specification recapitulates in vivo neural specification remains to be fully established. We pre...
journal_title：Stem cells and development
pub_type： 杂志文章
doi：10.1089/scd.2011.0593
更新日期：2012-10-10 00:00:00
abstract：:There have been reports of marrow cells converting into pulmonary epithelial cells after marrow transplantation in irradiated mice. We evaluated the impact of whole bone marrow (WBM) infusion in mice, with or without total body irradiation (TBI), treated with saline or monocrotaline (MCT), which induces pulmonary hype...
journal_title：Stem cells and development
pub_type： 杂志文章
doi：10.1089/scd.2008.0237
更新日期：2009-06-01 00:00:00
abstract：:Mesenchymal stem cells (MSC) possess a range of immunomodulatory properties which they exert through soluble mediators and through direct cell-cell contact. Due to these immune regulatory properties, the safety and clinical efficacy of MSC treatment has been tested in a number of autoimmune disorders. In this review w...
journal_title：Stem cells and development
pub_type： 杂志文章,评审
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更新日期：2015-09-15 00:00:00
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journal_title：Stem cells and development
pub_type： 杂志文章,评审
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journal_title：Stem cells and development
pub_type： 杂志文章
doi：10.1089/scd.2014.0318
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journal_title：Stem cells and development
pub_type： 杂志文章
doi：10.1089/scd.2018.0033
更新日期：2018-09-01 00:00:00
abstract：:Progenitor expansion during development is a highly regulated process dictating the final organ size, while expansion of specific progenitor populators can adjust the final cellular composition of the organ. Understanding factors involved in these pathways is required to develop cell-based therapies such as β-cell tra...
journal_title：Stem cells and development
pub_type： 杂志文章,评审
doi：10.1089/scd.2013.0106
更新日期：2013-11-15 00:00:00
abstract：:Betacellulin (BTC), a ligand of the epidermal growth factor receptor, has been shown to promote growth and differentiation of pancreatic β-cells and to improve glucose metabolism in experimental diabetic rodent models. Mesenchymal stem cells (MSCs) have been already proved to be multipotent. Recent work has attributed...
journal_title：Stem cells and development
pub_type： 杂志文章
doi：10.1089/scd.2009.0490
更新日期：2011-02-01 00:00:00
abstract：:Allergic asthma is an inflammatory airway disease caused by T helper type 2 (Th2)-driven immune responses. Recent studies have demonstrated that adipose-derived stromal cells (ASC) have an immunosuppressive effect on T-cell activity. This study was performed to investigate whether ASC can inhibit Th2-dependent allergi...
journal_title：Stem cells and development
pub_type： 杂志文章
doi：10.1089/scd.2009.0513
更新日期：2010-11-01 00:00:00
abstract：:Humans and nonhuman primates (NHPs) are similar in size, behavior, physiology, biochemistry, structure and function of organs, and complexity of the immune system. Research on NHPs generates complementary data that bridge translational research from small animal models to humans. NHP models of human disease offer uniq...
journal_title：Stem cells and development
pub_type： 杂志文章
doi：10.1089/scd.2014.0374
更新日期：2014-12-01 00:00:00
abstract：:Resistance to imatinib can occur in patients with chronic myelogenous leukemia (CML). In this study, we report mechanisms of action of histone deacetylase (HDAC) inhibitor, depsipeptide (FK228) in BCR/ABL-expressing cell lines and its effectiveness in imatinib-resistant cells from patients with blast crisis of CML. FK...
journal_title：Stem cells and development
pub_type： 杂志文章
doi：10.1089/scd.2007.9994
更新日期：2007-06-01 00:00:00
abstract：:Accumulating evidence suggests that the delivery of human adipose tissue-derived stromal cells (hASCs) has great potential as regenerative therapy. This was performed to develop a method for expanding hASCs by reducing the amount of serum required. We demonstrate that hASCs were able to expand efficiently in media con...
journal_title：Stem cells and development
pub_type： 杂志文章
doi：10.1089/scd.2008.0358
更新日期：2009-05-01 00:00:00
abstract：:In the seminiferous epithelium of the testis, Sertoli cells are key niche cells directing proliferation and differentiation of spermatogonial stem cells (SSCs) into spermatozoa. Sertoli cells produce glial cell line-derived neurotrophic factor (GDNF), which is essential for SSC self-renewal and progenitor expansion. W...
journal_title：Stem cells and development
pub_type： 杂志文章
doi：10.1089/scd.2016.0318
更新日期：2017-04-15 00:00:00
