Abstract:
:Since human mesenchymal stem cells (MSCs) are therapeutically attractive for tissue regeneration and repair, we examined the physiological responses of human umbilical cord blood-derived MSCs (hUCB-MSCs) to genotoxic stress. We found that that sublethal doses of reactive oxygen species (ROS) and ionizing radiation cause DNA damage and reduce DNA synthesis and cell proliferation in hUCB-MSCs, resulting in cellular senescence. In contrast, these physiological changes were limited in human fibroblast and cancer cells. Our data show that reduced activities of antioxidant enzymes, which may occur due to low gene expression levels, cause hUCB-MSCs to undergo cellular senescence in response to oxidative stress and ionizing radiation. Resistance of hUCB-MSCs to oxidative stresses was restored by increasing the intracellular antioxidant activity in hUCB-MSCs via exogenous addition of antioxidants. Therefore, the proliferation and fate of hUCB-MSCs can be controlled by exposure to oxidative stresses.
journal_name
Stem Cells Devjournal_title
Stem cells and developmentauthors
Ko E,Lee KY,Hwang DSdoi
10.1089/scd.2011.0284subject
Has Abstractpub_date
2012-07-20 00:00:00pages
1877-86issue
11eissn
1547-3287issn
1557-8534journal_volume
21pub_type
杂志文章abstract::N-methyl-d-aspartate receptors (NMDARs) are expressed abundantly in the brain and play an important role in the regulation of neuronal development, learning, memory, neurodegenerative diseases, and neurogenesis, all of which are extensively discussed in reviews published elsewhere. While the number of research article...
journal_title:Stem cells and development
pub_type: 杂志文章,评审
doi:10.1089/scd.2016.0325
更新日期:2017-06-01 00:00:00
abstract::In recent years it has been hypothesized that cancer stem cells (CSCs) are the actual driving force of tumor formation, highlighting the need to specifically target CSCs to successfully eradicate cancer growth and recurrence. Particularly, the deregulation of physiological signaling pathways controlling stem cell prol...
journal_title:Stem cells and development
pub_type: 杂志文章,评审
doi:10.1089/scd.2015.0081
更新日期:2015-10-15 00:00:00
abstract::The aryl hydrocarbon receptor (AhR) belongs to the basic helix-loop-helix family of DNA-binding proteins that play a role in the toxicity and carcinogenicity of certain chemicals. The most potent ligand of the AhR known is 2,3,7,8-tetracholorodibenzo-p-dioxin. We previously reported tetrachlorodibenzo-p-dioxin-induced...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2010.0333
更新日期:2011-05-01 00:00:00
abstract::Immunodeficient mice transplanted with human hematopoietic stem cells (HSCs) have been referred to as "Human Immune System" (HIS) mice and are a translational platform for studying human immune responses in vivo. Human HSC sources used in generating HIS mice include fetal liver (FL), umbilical cord blood (CB), and adu...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2017.0156
更新日期:2017-12-01 00:00:00
abstract::Embryonic stem (ES) cells are recognized as an excellent cell culture model for studying developmental mechanisms and their therapeutic modulations. The aim of this work was to define whether using magnetofection was an efficient way to manipulate stem cells genetically without adversely affecting their proliferation ...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2007.0064
更新日期:2008-02-01 00:00:00
abstract::Multipotent stem/progenitor cells from bone marrow stroma (mesenchymal stromal cells or MSCs) were previously shown to enhance proliferation and differentiation of neural stem cells (NSCs) in vivo, but the molecular basis of the effect was not defined. Here coculturing human MSCs (hMSCs) with rat NSCs (rNSCs) was foun...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2009.0444
更新日期:2011-02-01 00:00:00
abstract::The activation and transcriptional activity of signal transducer and activator of transcription 3 (STAT3) is essential for maintaining mouse embryonic stem (ES) cell cultures in an undifferentiated state. However, reports from human and monkey ES-cell culture suggest that STAT3 is dispensable for pluripotency in these...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2007.0262
更新日期:2009-01-01 00:00:00
abstract::Epoxyeicosatrienoic acid (EET) contributes to browning of white adipose stem cells to ameliorate obesity/diabetes and insulin resistance. In the current study, we show that EET altered preadipocyte function, enhanced peroxisome proliferation-activated receptor γ coactivator α (PGC-1α) expression, and increased mitocho...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2016.0072
更新日期:2016-07-15 00:00:00
abstract::Bone marrow mesenchymal stem cells (BMMSCs) have been used to treat a variety of autoimmune diseases in clinics. However, the therapeutic effects are largely dependent on the immunomodulatory capacity of culture-expanded BMMSCs. In the present study, we show that aspirin (acetylsalicylic acid, ASA)-treated BMMSCs have...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2014.0081
更新日期:2014-09-01 00:00:00
abstract::Induced pluripotent stem cells (iPSCs) are somatic cells that have been reprogrammed to a pluripotent state via introduction of defined transcription factors. iPSCs are a valuable resource for regenerative medicine, but whether iPSCs are identical to embryonic stem cells (ESCs) remains unclear. In this study, we perfo...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2011.0243
更新日期:2012-05-20 00:00:00
abstract::Recent evidence shows that amniotic fluid (AF) contains multiple cell types derived from the developing fetus, and may represent a novel source of stem cells for cell therapy. In this study, we examined the paracrine factors released by human amniotic fluid-derived mesenchymal stem cells (AF-MSCs) and their ability to...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2009.0138
更新日期:2010-06-01 00:00:00
abstract::The optimization of defined growth conditions is necessary for the development of clinical application of human embryonic stem cells (hESCs). Current research has focused on developing defined media formulations for long-term culture of hESCs with little attention on the establishment of defined substrates for hESC pr...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2008.0410
更新日期:2009-10-01 00:00:00
abstract::In this study we examine whether a somatic cell, once returned to a pluripotent state, gains the ability to reprogram other somatic cells. We reprogrammed mouse embryonic fibroblasts by viral induction of oct4, sox2, c-myc, and klf-4 genes. Upon fusion of the resulting iPS cells with somatic cells harboring an Oct4-GF...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2009.0142
更新日期:2010-02-01 00:00:00
abstract::Pericytes play a crucial role in angiogenesis and vascular maintenance. They can be readily identified in vivo and isolated as CD146(+)CD34(-) cells from various tissues. Whether these and other markers reliably identify pericytes in vitro is unclear. CD146(+)CD34(-) selected cells exhibit multilineage potential. Thus...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2012.0415
更新日期:2013-09-01 00:00:00
abstract::Heterotopic ossification (HO) is a common and debilitating complication of burns, traumatic brain injuries, and musculoskeletal trauma and surgery. Although the exact mechanism of ectopic bone formation is unknown, mesenchymal stem cells (MSCs) capable of osteogenic differentiation are known to play an essential role....
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2014.0291
更新日期:2015-01-15 00:00:00
abstract::Müller glia are responsible for the retina regeneration observed in zebrafish. Although the human retina harbors Müller glia with stem cell characteristics, there is no evidence that they regenerate the retina after disease or injury. Transforming growth factor-β (TGFβ) and Wnt signaling regulate retinal neurogenesis ...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2015.0262
更新日期:2016-01-01 00:00:00
abstract::Citrin deficiency (CD) is a recessive genetic disorder caused by mutations in the citrin gene SLC25A13. CD causes various symptoms related to nutrient metabolism such as urea cycle failure, abnormal amino acid levels, and fatty liver. To understand the pathophysiology of CD, the molecular phenotypes were investigated ...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2015.0342
更新日期:2016-04-15 00:00:00
abstract::Herein we review recent data that support host tolerance of allogeneic adult mesenchymal stem cells (MSC). Evidence is emerging that donor MSC deploy a very powerful array of mechanisms that allow escape from host allogeneic responses. These mechanisms include limited expression of alloantigen by the stem cell and cel...
journal_title:Stem cells and development
pub_type: 杂志文章,评审
doi:10.1089/scd.2005.14.252
更新日期:2005-06-01 00:00:00
abstract::Previously, we have shown that increasing the intracellular cGMP concentration enhances the sonic hedgehog (Shh) response in neural plate cells. The use of two mouse embryonic stem (ES) cell lines allowed a highly sensitive and reproducible quantification of the Shh response in neuralized embryoid bodies. Here we demo...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2006.15.647
更新日期:2006-10-01 00:00:00
abstract::The differentiation of human pluripotent stem cells to the B-cell lymphoid lineage has important clinical applications that include in vitro modeling of developmental lymphogenesis in health and disease. Here, we first demonstrate the capacity of human induced pluripotent stem cells (hiPSCs) to differentiate into CD14...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2014.0318
更新日期:2015-05-01 00:00:00
abstract::With the recent advances in regenerative medicine, nanotechnology has created a niche for itself as a promising avenue in this field. Innumerable studies have been carried out by researchers using virus-based methodologies for the purpose of epigenetic reprogramming. Although this method is ostensibly safe, nonetheles...
journal_title:Stem cells and development
pub_type: 杂志文章,评审
doi:10.1089/scd.2014.0309
更新日期:2015-03-01 00:00:00
abstract::Neuroblastoma (NB) is the second most common solid cancer in childhood, accounting for 15% of cancer-related deaths in children. In high-risk NB patients, the majority suffers from metastasis. Despite intensive multimodal treatment, long-term survival remains <40%. The bone marrow (BM) is among the most common sites o...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2020.0142
更新日期:2021-01-15 00:00:00
abstract::Stem cell therapy is a promising therapeutic strategy for treating myocardial infarction (MI). However, it is necessary to identify ideal adult stem cells for transplantation and explore mechanisms of the transplanted cells in improving cardiac functions after MI. In this study, a population of embryonic-like stem cel...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2011.0280
更新日期:2012-05-20 00:00:00
abstract::This study was undertaken to establish rat embryonic stem (ES) cells from parthenogenetically developing blastocysts. Ten blastocysts were prepared by treatment of ovulated rat oocytes with ionomycin and cycloheximide, and three alkaline phosphatase-positive ES cell lines were established using the N2B27 medium supple...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2013.0200
更新日期:2014-01-15 00:00:00
abstract::The mental retardation-associated protein, srGAP3 is highly expressed in neurogenic sites. It is thought to regulate the key aspects of neuronal development and functions. Little is known about the interaction between srGAP3 and immature neural stem cells/neural progenitor cells (NSCs/NPCs). In the current study, the ...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2012.0455
更新日期:2013-06-01 00:00:00
abstract::Traditionally, undifferentiated pluripotent human embryonic and induced pluripotent stem cells (hESCs and hiPSCs) have been expanded as monolayer colonies in adhesion culture, both in the presence or absence of feeder cells. However, the use of pluripotent stem cells poses the need to scale-up current culture methods....
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2010.0517
更新日期:2011-11-01 00:00:00
abstract::In this retrospective study, 209 patients with malignant disease were analyzed for levels of T-cell receptor excision circles (TRECs) for the first 24 months after allogeneic stem cell transplantation. CD3(+) cells were separated by direct antibody-coupled magnetic beads, followed by DNA extraction according to a stan...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2013.0588
更新日期:2014-07-15 00:00:00
abstract::Smooth muscle cell (SMC) differentiation and dedifferentiation play a critical role in the pathogenesis of cardiovascular diseases. The lack of a good and simple in vitro SMC differentiation system has hampered the progress of SMC field for years. The generation of such an in vitro system would be invaluable for explo...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2008.0179
更新日期:2009-06-01 00:00:00
abstract::In this study, we isolated and characterized spontaneously differentiated human embryonic stem cells (SD-hESCs) found in hESC colonies in comparison to the morphologically premature ESCs in the colonies to investigate the potential role of SD-hESCs in embryogenesis. SD-hESCs were distinguished from undifferentiated hE...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2013.0083
更新日期:2013-10-15 00:00:00
abstract::The induction and detection of chemotactic migration in mesenchymal stem cells (MSC) are complicated by their adherent nature. We describe here the experimental details for inducing the transmigration of MSC seeded in microchemotaxis chambers and reliably identifying the translocated cells. A combination of 8-microm p...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2005.14.231
更新日期:2005-04-01 00:00:00