Simple, efficient, and reproducible gene transfection of mouse embryonic stem cells by magnetofection.

abstract：:Progenitor expansion during development is a highly regulated process dictating the final organ size, while expansion of specific progenitor populators can adjust the final cellular composition of the organ. Understanding factors involved in these pathways is required to develop cell-based therapies such as β-cell tra...

journal_title：Stem cells and development

authors： Belo J,Krishnamurthy M,Oakie A,Wang R

更新日期：2013-11-15 00:00:00

abstract：:Somatic cells can be reprogrammed to induced pluripotent stem cells (iPSCs) by defined factors. However, substantial cell numbers subjected to iPSC induction stray from the main reprogramming route and are immortalized as partial iPSCs. These partial iPSCs can become genuine iPSCs by exposure to the ground state condi...

journal_title：Stem cells and development

authors： Kamon M,Katano M,Hiraki-Kamon K,Hishida T,Nakachi Y,Mizuno Y,Okazaki Y,Suzuki A,Hirasaki M,Ueda A,Nishimoto M,Kato H,Okuda A

更新日期：2014-09-15 00:00:00

abstract：:Although the pluripotent and proliferative capacity of embryonic germ (EG) cells is thought to be equivalent to that of embryonic stem (ES) cells, there has been far less attention focused on the potential use of EG cells for applications in developing novel strategies of tissue transplantation in the treatment of deg...

journal_title：Stem cells and development

authors： Wu Y,Ge C,Zeng W,Zhang C

更新日期：2010-02-01 00:00:00

abstract：:Bisphosphonate-related osteonecrosis of the jaws (BRONJ) is a severe adverse reaction, which results in progressive bone destruction in the maxillofacial region of patients. To date, the pathological mechanisms remain largely unclear. Recently, we found that BRONJ patient had significantly deep periodontal pockets and...

journal_title：Stem cells and development

authors： Li M,Yu Y,Shi Y,Zhou Y,Zhang W,Hua H,Ge J,Zhang Z,Ye D,Yang C,Wang S

更新日期：2020-02-01 00:00:00

abstract：:Immunodeficient mice transplanted with human hematopoietic stem cells (HSCs) have been referred to as "Human Immune System" (HIS) mice and are a translational platform for studying human immune responses in vivo. Human HSC sources used in generating HIS mice include fetal liver (FL), umbilical cord blood (CB), and adu...

journal_title：Stem cells and development

authors： Walker JA,Vuyyuru R,Manser T,Alugupalli KR

更新日期：2017-12-01 00:00:00

abstract：:Protein tyrosine phosphatases have been recognized as critical components of multiple signaling regulators of fundamental cellular processes, including differentiation, cell death, and migration. In this study, we show that dual specificity phosphatase 4 (DUSP4) is crucial for neuronal differentiation and functions in...

journal_title：Stem cells and development

authors： Kim SY,Han YM,Oh M,Kim WK,Oh KJ,Lee SC,Bae KH,Han BS

更新日期：2015-03-15 00:00:00

abstract：:Reduction of estradiol production and high serum concentrations of follicular stimulating hormone (FSH) are endocrine disorders associated with premature ovarian failure. Here, we report that transplantation of ovarian-like cells differentiated from stem cells restored endogenous serum estradiol levels. Stem cells wer...

journal_title：Stem cells and development

authors： Park BW,Pan B,Toms D,Huynh E,Byun JH,Lee YM,Shen W,Rho GJ,Li J

更新日期：2014-07-15 00:00:00

abstract：:Primordial germ cells (PGCs), the precursors of gametes, have regulatory mechanisms involving transcription factors and epigenetic modifications. The transcription factor NANOG is a key regulator of germ cell and embryonic stem cell characteristics. However, the epigenetic regulation of NANOG with a histone deacetylas...

journal_title：Stem cells and development

authors： Jung HG,Hwang YS,Park YH,Cho HY,Rengaraj D,Han JY

更新日期：2018-09-01 00:00:00

abstract：:Members of the homeobox (Hox) gene family are known to mediate expansion of hematopoietic stem cells (HSCs) and progenitors. The absence of oncogenic properties promoted HOXB4 as prime candidate in the quest to expand HSCs for clinical purposes. Despite its potential to expand HSCs, studies with mutant mice showed tha...

journal_title：Stem cells and development

authors： Fournier M,Lebert-Ghali CÉ,Krosl G,Bijl JJ

更新日期：2012-01-01 00:00:00

abstract：:Cyclooxygenase-2 (COX-2) inhibitors suppress bone repair and bone formation by suppressing angiogenesis as well as potentially interfering with osteoblast and osteoclast functions. In spite of these reports, there is a controversy over the exact effects of COX-2 inhibitors on bone formation processes itself. This work...

journal_title：Stem cells and development

authors： Yoon DS,Yoo JH,Kim YH,Paik S,Han CD,Lee JW

更新日期：2010-10-01 00:00:00

abstract：:There is surprising confusion surrounding the concept of biological totipotency, both within the scientific community and in society at large. Increasingly, ethical objections to scientific research have both practical and political implications. Ethical controversy surrounding an area of research can have a chilling ...

journal_title：Stem cells and development

authors： Condic ML

更新日期：2014-04-15 00:00:00

abstract：:Extraembryonic endoderm (XEN) cells are stem cell lines derived from primitive endoderm cells of inner cell mass in blastocysts. These cells have self-renewal properties and differentiate into visceral endoderm (VE) and parietal endoderm (PE) of the yolk sac. Recently, it has been reported that XEN cells can contribut...

journal_title：Stem cells and development

authors： Nishimura T,Unezaki N,Kanegi R,Wijesekera DPH,Hatoya S,Sugiura K,Kawate N,Tamada H,Imai H,Inaba T

更新日期：2017-08-01 00:00:00

abstract：:Several reports have shown that the expression of Sca-1 (Ly-6A/E), the most widely used murine hematopoietic stem cell marker, is restricted to blood vessels in several nonhematopoietic tissues. However, there is no information about which components are expressing Sca-1, and what the role of Sca-1 could be. Because w...

journal_title：Stem cells and development

authors： Luna G,Paez J,Cardier JE

更新日期：2004-10-01 00:00:00

abstract：:Ex vivo cultures of human bone marrow-derived mesenchymal stem cells (MSCs) contain subsets of progenitors exhibiting dissimilar properties. One of these subsets comprises uncommitted progenitors displaying distinctive features, such as morphology, a quiescent condition, growth factor production, and restricted tissue...

journal_title：Stem cells and development

authors： Minguell JJ,Fierro FA,Epuñan MJ,Erices AA,Sierralta WD

更新日期：2005-08-01 00:00:00

abstract：:Previously, we have shown that increasing the intracellular cGMP concentration enhances the sonic hedgehog (Shh) response in neural plate cells. The use of two mouse embryonic stem (ES) cell lines allowed a highly sensitive and reproducible quantification of the Shh response in neuralized embryoid bodies. Here we demo...

journal_title：Stem cells and development

authors： Christensen C,Zhang S,Roelink H

更新日期：2006-10-01 00:00:00

abstract：:Myocardial infarction is a major public health problem that causes significant mortality despite recent advances in its prevention and treatment. Therefore, approaches based on adult stem cells represent a promising alternative to conventional therapies for this life-threatening condition. Mesenchymal stem cells (MSCs...

journal_title：Stem cells and development

authors： Armiñán A,Gandía C,Bartual M,García-Verdugo JM,Lledó E,Mirabet V,Llop M,Barea J,Montero JA,Sepúlveda P

更新日期：2009-07-01 00:00:00

abstract：:Remyelination is the goal of potential cell transplantation therapies for demyelinating diseases and other central nervous system injuries. Transplantation of oligodendrocyte precursor cells (OPCs) can result in remyelination in the central nervous system, and induced pluripotent stem cells (iPSCs) are envisioned to b...

journal_title：Stem cells and development

authors： Umebayashi D,Coles B,van der Kooy D

更新日期：2016-05-01 00:00:00

abstract：:N-methyl-d-aspartate receptors (NMDARs) are expressed abundantly in the brain and play an important role in the regulation of neuronal development, learning, memory, neurodegenerative diseases, and neurogenesis, all of which are extensively discussed in reviews published elsewhere. While the number of research article...

journal_title：Stem cells and development

authors： Chakraborty A,Murphy S,Coleman N

更新日期：2017-06-01 00:00:00

abstract：:A murine stromal cell line (OP9-DL1) expressing a notch ligand, Delta-like-1, has been shown to be able to drive the differentiation of both murine and human hematopoietic progenitors into T cells in vitro. Further studies showed that hematopoietic progenitors transduced by a retroviral vector to express a human CD8 T...

journal_title：Stem cells and development

authors： Dai B,Wang P

更新日期：2009-03-01 00:00:00

abstract：:Adipose-derived stem cells (ADSCs) are a type of multipotent mesenchymal stem cells with immunosuppressive capacities. However, the underlying mechanisms involved in the inhibitory effects of ADSCs on T cells are not completely elucidated. In this study, human peripheral blood mononuclear cells (PBMCs) stimulated with...

journal_title：Stem cells and development

authors： Zhou K,Guo S,Tong S,Sun Q,Li F,Zhang X,Qiao Y,Liang G

更新日期：2018-09-01 00:00:00

abstract：:Pluripotent human embryonic stem cells (hESCs) provide appropriate systems for developmental studies and prospective donor cell sources for regenerative medicine. Identification of surface markers specific to hESCs is a prerequisite for studying hESC biology and can be used to generate clinical-level donor cell prepar...

journal_title：Stem cells and development

authors： Choi HS,Kim WT,Kim H,Kim JJ,Ko JY,Lee SW,Jang YJ,Kim SJ,Lee MJ,Jung HS,Kzhyshkowska J,Um SJ,Lee MY,Lee SH,Kim CH,Ryu CJ

更新日期：2011-04-01 00:00:00

abstract：:Rare heterozygous deletions in the neurexin 1 (NRXN1) gene robustly increase an individual's risk of developing neurological and psychiatric disorders. However, the molecular bases by which different mutations result in different clinical presentations, with variable penetrance, are unknown. To better understand the m...

journal_title：Stem cells and development

authors： De Los Angeles A,Tunbridge EM

更新日期：2020-09-01 00:00:00

abstract：:Multipotent mesenchymal stromal cells (MSCs) are more and more frequently used to treat orthopedic injuries in horses. However, these cells are limited in their expandability and differentiation capacity. Recently, the first equine-induced pluripotent stem cell (iPSC) lines were reported by us [ 1 ]. In vitro differen...

journal_title：Stem cells and development

authors： Lepage SI,Nagy K,Sung HK,Kandel RA,Nagy A,Koch TG

更新日期：2016-01-01 00:00:00

abstract：:Dental follicle stem cells (DFSCs), which are the stem cells present in the dental follicle tissue of the tooth germ and are derived from the neural crest, are direct precursor cells of periodontal tissues and can form the periodontal ligament, cementum, and alveolar bone proper in the late stage of tooth development....

journal_title：Stem cells and development

authors： Zhang J,Ding H,Liu X,Sheng Y,Liu X,Jiang C

更新日期：2019-08-01 00:00:00

abstract：:Cardiac stem/progenitors are being used in the clinic to treat patients with a range of cardiac pathologies. However, improvements in heart function following treatment have been reported to be variable, with some showing no response. This discrepancy in response remains unresolved. Mesenchymal stem cells (MSCs) have ...

journal_title：Stem cells and development

authors： Oldershaw R,Owens WA,Sutherland R,Linney M,Liddle R,Magana L,Lash GE,Gill JH,Richardson G,Meeson A

更新日期：2019-05-01 00:00:00

abstract：:The clinical use of stem cells in cell-based therapeutics for degenerative diseases requires development of criteria for defining normal stem cells to ensure safe transplantation. Currently, identification of abnormal from normal stem cells is based on extensive ex vivo and in vivo testing. Raman microscopy is a label...

journal_title：Stem cells and development

authors： Harkness L,Novikov SM,Beermann J,Bozhevolnyi SI,Kassem M

更新日期：2012-08-10 00:00:00

abstract：:Multipotent mesenchymal stem/stromal cells (MSCs) are strongly involved in tissue homeostasis mainly through paracrine regulation. In this study, we examined the influence of simulated microgravity on the angiogenic potential of adipose-derived MSCs (ASCs). The conditioned medium (CM) from random positioning machine (...

journal_title：Stem cells and development

authors： Ratushnyy A,Ezdakova M,Yakubets D,Buravkova L

更新日期：2018-06-15 00:00:00

abstract：:The optimization of defined growth conditions is necessary for the development of clinical application of human embryonic stem cells (hESCs). Current research has focused on developing defined media formulations for long-term culture of hESCs with little attention on the establishment of defined substrates for hESC pr...

journal_title：Stem cells and development

authors： Brafman DA,Shah KD,Fellner T,Chien S,Willert K

更新日期：2009-10-01 00:00:00

abstract：:In the central nervous system (CNS), generation of phenotypic diversity within the neuronal lineage is precisely regulated in a spatial and temporal fashion. Neural basic helix-loop-helix (bHLH) transcription factors are cell intrinsic factors that control commitment to neuronal lineage and play an important role in n...

journal_title：Stem cells and development

authors： Kumar M,Bagchi B,Gupta SK,Meena AS,Gressens P,Mani S

更新日期：2007-08-01 00:00:00

abstract：:Bone marrow human mesenchymal stem cells (hMSCs) are known to survive in serum-free media, when most normal somatic cells do not survive. We found that the endogenously-activated bone morphogenetic protein (BMP) pathway is involved in this cellular behavior. Under this culture condition, phosphorylated Smad1 (PSmad1),...

journal_title：Stem cells and development

authors： Solmesky LJ,Abekasis M,Bulvik S,Weil M

更新日期：2009-11-01 00:00:00