Abstract:
:Ex vivo cultures of human bone marrow-derived mesenchymal stem cells (MSCs) contain subsets of progenitors exhibiting dissimilar properties. One of these subsets comprises uncommitted progenitors displaying distinctive features, such as morphology, a quiescent condition, growth factor production, and restricted tissue biodistribution after transplantation. In this study, we assessed the competence of these cells to express, in the absence of differentiation stimuli, markers of mesoderm and ectodermic (neural) cell lineages. Fluorescence microscopy analysis showed a unique pattern of expression of osteogenic, chondrogenic, muscle, and neural markers. The depicted "molecular signature" of these early uncommitted progenitors, in the absence of differentiation stimuli, is consistent with their multipotentiality and plasticity as suggested by several in vitro and in vivo studies.
journal_name
Stem Cells Devjournal_title
Stem cells and developmentauthors
Minguell JJ,Fierro FA,Epuñan MJ,Erices AA,Sierralta WDdoi
10.1089/scd.2005.14.408keywords:
subject
Has Abstractpub_date
2005-08-01 00:00:00pages
408-14issue
4eissn
1547-3287issn
1557-8534journal_volume
14pub_type
杂志文章abstract::Tumor necrosis factor-α (TNF-α) has multiple effects on proliferation and differentiation of human mesenchymal stem cells. Transforming growth factor-activated kinase-1 (TAK1) mediates the activation of nuclear factor-kappa B (NF-κB), c-Jun N-terminal kinase (JNK), and p38 pathways in response to TNF-α. However, the r...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2014.0272
更新日期:2015-04-01 00:00:00
abstract::Differential staining is an immunocytochemical staining that visualizes trophectoderm (TE) and the inner cell mass (ICM) of the blastocysts. It is used to determine the blastocyst quality, but could also be a useful tool to assess the integration site of injected cells into the early embryo. This is relevant for testi...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2014.0503
更新日期:2015-05-01 00:00:00
abstract::Traumatic spinal cord injury (SCI) leads to a cascade of secondary chemical insults, including oxidative stress and glutamate excitotoxicity, which damage host neurons and glia. Transplantation of exogenous neural stem/progenitor cells (NSPCs) has shown promise in enhancing regeneration after SCI, although survival of...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2015.0389
更新日期:2016-08-15 00:00:00
abstract::Cyclooxygenase-2 (COX-2) inhibitors suppress bone repair and bone formation by suppressing angiogenesis as well as potentially interfering with osteoblast and osteoclast functions. In spite of these reports, there is a controversy over the exact effects of COX-2 inhibitors on bone formation processes itself. This work...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2009.0393
更新日期:2010-10-01 00:00:00
abstract::Immunodeficient mice transplanted with human hematopoietic stem cells (HSCs) have been referred to as "Human Immune System" (HIS) mice and are a translational platform for studying human immune responses in vivo. Human HSC sources used in generating HIS mice include fetal liver (FL), umbilical cord blood (CB), and adu...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2017.0156
更新日期:2017-12-01 00:00:00
abstract::Tendons and ligaments (T/L) are dense connective tissues of mesodermal origin. During embryonic development, the tendon-specific cells descend from a sub-set of mesenchymal progenitors condensed in the syndetome, a dorsolateral domain of the sclerotome. These cells are defined by the expression of the transcription fa...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2011.0150
更新日期:2012-04-10 00:00:00
abstract::Cell mobilization, a process that influences circulation, margination, and finally, homing play key roles in the regeneration processes mediated by stem cells. Recent studies as well as prior studies from our group indicate an important role of the spleen in hematopoietic reconstitution, but to date the role of the sp...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2011.0068
更新日期:2012-03-20 00:00:00
abstract::Esophagus and trachea arise from a common origin, the anterior foregut tube. The compartmentalization process of the foregut into the esophagus and trachea is still poorly understood. Esophageal atresia/tracheoesophageal fistula (EA/TEF) is one of the most common gastrointestinal congenital defects with an incidence r...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2020.0075
更新日期:2020-08-01 00:00:00
abstract::Spiral ganglion neurons (SGNs) are usually damaged in sensorineural hearing loss. SGN-derived neural stem cells (NSCs) have been identified and proposed to differentiate into neurons to replace damaged SGNs. However, it remains obscure whether SGN-NSC-derived neurons (ScNs) are electrophysiologically functional and po...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2015.0345
更新日期:2016-05-15 00:00:00
abstract::Herein we review recent data that support host tolerance of allogeneic adult mesenchymal stem cells (MSC). Evidence is emerging that donor MSC deploy a very powerful array of mechanisms that allow escape from host allogeneic responses. These mechanisms include limited expression of alloantigen by the stem cell and cel...
journal_title:Stem cells and development
pub_type: 杂志文章,评审
doi:10.1089/scd.2005.14.252
更新日期:2005-06-01 00:00:00
abstract::Members of helix-loop-helix (HLH) protein family of Id (inhibitor of differentiation) dimerize with bHLH transcription factors and function as negative regulators of differentiation during development. Most of inhibitory roles of Id proteins have been demonstrated in non-neural tissues, and their roles in the developi...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2009.0093
更新日期:2010-06-01 00:00:00
abstract::Non-tumorous liver tissue removed during surgery to resect hepatocellular carcinoma (HCC) is potentially a useful source of material from which cells, particularly liver progenitor/stem cells (LPCs), can be isolated to establish cell lines. The purpose of this study was to evaluate the applicability of the "plate-and-...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2009.0304
更新日期:2010-08-01 00:00:00
abstract::Adipose tissue (AT) is composed of mature adipocytes and stromal vascular fraction (SVF) cells, including adipose stem/stromal cells (ASCs). We characterized hematopoietic cells residing in human nonobese AT by analyzing the SVF isolated from human lipoaspirates and peripheral blood (PB). Flow cytometry revealed that ...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2012.0442
更新日期:2013-03-15 00:00:00
abstract::Human umbilical cord blood (CB) has been used successfully in stem cell transplantation. A subpopulation of CD34(+) cells expresses chemokine receptor CXCR4 which is critical for bone marrow engraftment in human hematopoietic stem cells. Here, we demonstrate the effect of short-term culture on CXCR4 expression on umbi...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2008.0298
更新日期:2009-10-01 00:00:00
abstract::In this work, we evaluated the expansion of human hematopoietic stem cells from umbilical cord blood in roller bottles. The Iscove's modified Dulbecco's medium, the Stem Pro 34-SFM medium, and the L-15 Leibovitz's medium for cultures in CO(2)-free atmosphere were assessed. At day 5 of culture, total colony forming uni...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2010.0236
更新日期:2011-04-01 00:00:00
abstract::In this retrospective study, 209 patients with malignant disease were analyzed for levels of T-cell receptor excision circles (TRECs) for the first 24 months after allogeneic stem cell transplantation. CD3(+) cells were separated by direct antibody-coupled magnetic beads, followed by DNA extraction according to a stan...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2013.0588
更新日期:2014-07-15 00:00:00
abstract::Tissue engineering and cell implantation therapies are gaining popularity because of their potential to repair and regenerate tissues and organs. To investigate the role of inflammatory cytokines in new tissue development in engineered tissues, we have characterized the nature and timing of cell populations forming ne...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2012.0451
更新日期:2013-05-15 00:00:00
abstract::The success rate of human embryonic stem cell (hESC) derivation depends on both culture conditions and embryo quality and is routinely determined by morphological criteria. However, high incidence of chromosomal abnormality even in high-grade cleavage embryos from in vitro fertilization (IVF) patients suggests that th...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2009.0136
更新日期:2010-01-01 00:00:00
abstract::CD41 (αIIb integrin), a specific marker for megakaryocytes and platelets, was recently shown to mark the initiation of definitive hematopoiesis in mouse embryos. However, whether embryonic CD41(+) populations have a nonhematopoietic potential remains elusive. Here, we report that the CD41(+) cells from the mouse E11.0...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2011.0572
更新日期:2012-09-20 00:00:00
abstract::Studies on rodent bone marrow stromal cells (MSCs) have revealed a capacity, for at least a portion of cells, to express neuron-like traits after differentiation in culture. Little, however, is known about the ability of human MSCs in this regard. We show here that incubation with certain differentiation cocktails, pa...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2004.13.625
更新日期:2004-12-01 00:00:00
abstract::Hematopoietic stem cells (HSCs) possess a distinct ability to perpetuate through self-renewal and to generate progeny that differentiate into mature cells of myeloid and lymphoid lineages. A better understanding of the molecular mechanisms by which HSCs replicate and differentiate from the perspective of developing ne...
journal_title:Stem cells and development
pub_type: 杂志文章,评审
doi:10.1089/scd.2006.15.755
更新日期:2006-12-01 00:00:00
abstract::Misexpression of stem cell-related genes may occur in some cancer cells, influencing patient's prognosis. This is the case of medulloblastoma, a common and clinically challenging malignant tumor of the central nervous system, where expression of the pluripotency factor, OCT4, is correlated with poor survival. A downst...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2015.0052
更新日期:2015-11-15 00:00:00
abstract::One of the challenges in stem cell research is to avoid transformation during cultivation. We studied high passage subventricular zone derived neural stem cells (NSCs) cultures of adult rats in the absence of growth factors epidermal growth factor (EGF) and basic fibroblast growth factor (bFGF). We termed this culture...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2009.0416
更新日期:2010-07-01 00:00:00
abstract::Fluorescence in situ hybridization (FISH) was used to analyze peripheral blood stem cells (PBSC) and stem cell assays (SCA) derived from them in 2 patients with acute myeloid leukemia (AML) with trisomy 8 as the sole chromosome abnormality prior to undergoing autologous stem cell transplantation. In both cases, the de...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/154732804773099227
更新日期:2004-02-01 00:00:00
abstract::We recently reported the isolation and characterization of a population of pancreatic progenitor cells (PPCs) from early trimester human fetal pancreata. The PPCs, being the forerunners of adult pancreatic cell lineages, were amenable to growth and differentiation into insulin-secreting islet-like cell clusters (ICCs)...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2008.0325
更新日期:2009-09-01 00:00:00
abstract::The field of regenerative medicine has increasingly recognized the importance to be inspired by developmental processes to identify signaling pathways crucial for 3D organogenesis and tissue regeneration. Here, we aimed at recapitulating the first events occurring during limb development (ie, cell condensation and exp...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2013.0216
更新日期:2013-11-01 00:00:00
abstract::Bone morphogenetic protein-2 (BMP-2) is a novel differentiation factor that is capable of inducing osteoblast differentiation and bone formation, making it an attractive option in treatment of bone defects, fractures, and spine fusions. Inflammation, which was a common situation during bone healing, is recognized to i...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2013.0345
更新日期:2014-02-01 00:00:00
abstract::In this study we examine whether a somatic cell, once returned to a pluripotent state, gains the ability to reprogram other somatic cells. We reprogrammed mouse embryonic fibroblasts by viral induction of oct4, sox2, c-myc, and klf-4 genes. Upon fusion of the resulting iPS cells with somatic cells harboring an Oct4-GF...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2009.0142
更新日期:2010-02-01 00:00:00
abstract::Resveratrol is a natural polyphenol antioxidant that has been shown to facilitate osteogenic differentiation. A recent breakthrough has demonstrated that ectopic expression of four genes is sufficient to reprogram murine and human fibroblasts into induced pluripotent stem (iPS) cells. However, the roles of resveratrol...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2009.0186
更新日期:2010-02-01 00:00:00
abstract::Human embryonic stem cells (hESCs) self-renew indefinitely as highly organized pluripotent colonies. Unlike mouse pluripotent stem cell colonies, human colonies form a uniform, flat, epithelium-like monolayer. Interestingly, it has been reported that colony morphology is closely correlated with the maintenance of plur...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2012.0288
更新日期:2013-01-15 00:00:00