Pertussis toxin enhances colony organization of enzymatic-dissociated single human embryonic stem cells.

abstract：:A long-term goal of mesenchymal progenitor cell (MPC) research is to identify cell-surface markers to facilitate MPC isolation. One reported MPC feature in humans and other species is lack of CD14 (lipopolysaccharide receptor) expression. The aim of this study was to evaluate CD14 as an MPC sorting marker. Our hypothe...

journal_title：Stem cells and development

authors： Hackett CH,Flaminio MJ,Fortier LA

更新日期：2011-04-01 00:00:00

abstract：:With the recent advances in regenerative medicine, nanotechnology has created a niche for itself as a promising avenue in this field. Innumerable studies have been carried out by researchers using virus-based methodologies for the purpose of epigenetic reprogramming. Although this method is ostensibly safe, nonetheles...

journal_title：Stem cells and development

authors： Kadengodlu PA,Rai VR

更新日期：2015-03-01 00:00:00

abstract：:Herein we review recent data that support host tolerance of allogeneic adult mesenchymal stem cells (MSC). Evidence is emerging that donor MSC deploy a very powerful array of mechanisms that allow escape from host allogeneic responses. These mechanisms include limited expression of alloantigen by the stem cell and cel...

journal_title：Stem cells and development

authors： Barry FP,Murphy JM,English K,Mahon BP

更新日期：2005-06-01 00:00:00

abstract：:The major reservoir of human multipotent mesenchymal stem/stromal cells (MSCs) is the bone marrow (BM) with the capability to control hematopoietic stem cell development. The regenerative potential of MSCs is associated with enhanced endogenous repair and healing mechanisms that modulate inflammatory responses. Our pr...

journal_title：Stem cells and development

authors： Bacskai I,Mázló A,Kis-Tóth K,Szabó A,Panyi G,Sarkadi B,Apáti Á,Rajnavölgyi É

更新日期：2015-08-01 00:00:00

abstract：:Gap junctions within extracellular matrix (ECM)-defined boundaries ensure synchronous activity between cells destined to become functional mediators that regulate cell behavior. However, the role of ECM in connexin (Cx) function in mouse embryonic stem cells (mESCs) has not been elucidated. Therefore, we examined the ...

journal_title：Stem cells and development

authors： Suh HN,Kim MO,Han HJ

更新日期：2012-07-20 00:00:00

abstract：:Citrin deficiency (CD) is a recessive genetic disorder caused by mutations in the citrin gene SLC25A13. CD causes various symptoms related to nutrient metabolism such as urea cycle failure, abnormal amino acid levels, and fatty liver. To understand the pathophysiology of CD, the molecular phenotypes were investigated ...

journal_title：Stem cells and development

authors： Kim Y,Choi JY,Lee SH,Lee BH,Yoo HW,Han YM

更新日期：2016-04-15 00:00:00

abstract：:Although umbilical cord blood (UCB) has been widely accepted as an alternative source of hematopoietic stem cells (HSC) for transplantation, its use in adults is restricted because of low absolute HSC numbers. To overcome this obstacle, expansion of HSC in coculture with feeder cells is a promising possibility. In thi...

journal_title：Stem cells and development

authors： Magin AS,Körfer NR,Partenheimer H,Lange C,Zander A,Noll T

更新日期：2009-01-01 00:00:00

abstract：:The quality of hematopoietic stem cells (HSCs) is essentially defined by two characteristics, i.e., multilineage differentiation and self-renewal capacity. Thus, it is of high priority to clarify mechanisms that regulate these functions and to understand them at the molecular level. In the present study, we investigat...

journal_title：Stem cells and development

authors： Schraml E,Voglauer R,Fortschegger K,Sibilia M,Stelzer I,Grillari J,Schauenstein K

更新日期：2008-04-01 00:00:00

abstract：:The development of methods to induce differentiation of human mesenchymal stem cells (hMSCs) has opened the possibility of using these cells in regenerative or reparative therapies. However, the low frequency of hMSCs in tissue means it is often necessary to expand these cells extensively in vitro. In this study, we e...

journal_title：Stem cells and development

authors： Khoo ML,Shen B,Tao H,Ma DD

更新日期：2008-10-01 00:00:00

abstract：:My concept of cell differentiation involves genetic information from DNA being transcribed into mRNA proteins-morphogenes (mRNAs+ homeodomain proteins)-and stored in the ovoplasm as maternal inheritance, or cytoplasmic genetic memory. Feedback mechanism(s) allow these morphogenes to selectively unlock new genes, regul...

journal_title：Stem cells and development

authors： Kristeva MA

更新日期：2008-10-01 00:00:00

abstract：:Chronic idiopathic neutropenia (CIN) is a granulopoiesis disorder associated with an inhibitory bone marrow (BM) microenvironment consisting of activated T-lymphocytes and pro-inflammatory mediators. In this study, we investigated the possible involvement of BM mesenchymal stem cells (MSCs) in the pathophysiology of C...

journal_title：Stem cells and development

authors： Stavroulaki E,Kastrinaki MC,Pontikoglou C,Eliopoulos D,Damianaki A,Mavroudi I,Pyrovolaki K,Katonis P,Papadaki HA

更新日期：2011-08-01 00:00:00

abstract：:Osteophytes are a distinct feature of osteoarthritis (OA). Their formation may be related to pluripotential cells in the periosteum responding to stimulus during OA. This study aimed to isolate stem cells from osteophyte tissues and to characterize their phenotype, proliferation, and differentiation potential, as well...

journal_title：Stem cells and development

authors： Singh S,Jones BJ,Crawford R,Xiao Y

更新日期：2008-04-01 00:00:00

abstract：:Special features of mesenchymal stem cells (MSCs) have made them a popular tool in cell therapy and tissue engineering. Although mouse animal models and murine MSCs are common tools in this field, our understanding of the effect of in vitro expansion on the behavior of these cells is poor and controversial. In additio...

journal_title：Stem cells and development

authors： Ahmadbeigi N,Soleimani M,Gheisari Y,Vasei M,Amanpour S,Bagherizadeh I,Shariati SA,Azadmanesh K,Amini S,Shafiee A,Arabkari V,Nardi NB

更新日期：2011-08-01 00:00:00

abstract：:The AMP-activated protein kinase (AMPK) mediates rapid, stress-induced loss of the inhibitor of differentiation (Id)2 in blastocysts and trophoblast stem cells (TSC), and a lasting differentiation in TSC. However, it is not known if AMPK regulates other potency factors or regulates them before the blastocyst stage. Th...

journal_title：Stem cells and development

authors： Xie Y,Awonuga A,Liu J,Rings E,Puscheck EE,Rappolee DA

更新日期：2013-05-15 00:00:00

abstract：:The aryl hydrocarbon receptor (AhR) belongs to the basic helix-loop-helix family of DNA-binding proteins that play a role in the toxicity and carcinogenicity of certain chemicals. The most potent ligand of the AhR known is 2,3,7,8-tetracholorodibenzo-p-dioxin. We previously reported tetrachlorodibenzo-p-dioxin-induced...

journal_title：Stem cells and development

authors： Singh KP,Garrett RW,Casado FL,Gasiewicz TA

更新日期：2011-05-01 00:00:00

abstract：:To better understand the basis of variation in cellular reprogramming, we performed experiments with two primary objectives: first, to determine the degree of difference, if any, in reprogramming efficiency among cells lines of a similar type after accounting for technical variables, and second, to compare the efficie...

journal_title：Stem cells and development

authors： Alicea B,Murthy S,Keaton SA,Cobbett P,Cibelli JB,Suhr ST

更新日期：2013-10-01 00:00:00

abstract：:Preclinical studies suggest that stem cell therapy (SCT) may improve poststroke recovery, and clinical trials investigating safety are ongoing. However, knowledge about patients' attitudes to SCT in stroke is limited. We evaluated the knowledge and attitudes to this therapeutic approach as well as possible factors inf...

journal_title：Stem cells and development

authors： Aked J,Delavaran H,Lindvall O,Norrving B,Kokaia Z,Lindgren A

更新日期：2017-04-15 00:00:00

abstract：:Betacellulin (BTC), a ligand of the epidermal growth factor receptor, has been shown to promote growth and differentiation of pancreatic β-cells and to improve glucose metabolism in experimental diabetic rodent models. Mesenchymal stem cells (MSCs) have been already proved to be multipotent. Recent work has attributed...

journal_title：Stem cells and development

authors： Paz AH,Salton GD,Ayala-Lugo A,Gomes C,Terraciano P,Scalco R,Laurino CC,Passos EP,Schneider MR,Meurer L,Cirne-Lima E

更新日期：2011-02-01 00:00:00

abstract：:Human embryonic stem cell (hESC)-derived natural killer (NK) cells are a promising source of antitumor lymphocytes for immunotherapeutics. They also provide a genetically tractable platform well suited for the study of antitumor immunotherapies in preclinical models. We have previously demonstrated the potency of hESC...

journal_title：Stem cells and development

authors： Knorr DA,Bock A,Brentjens RJ,Kaufman DS

更新日期：2013-07-01 00:00:00

abstract：:The aim of this study was to clarify the mechanisms that regulate hematopoietic cell expansion in vitro by identifying defined culture conditions. We report the results of experiments with CD34(+) cells from cord blood (CB, n = 13), bone marrow (BM, n = 4), and mobilized peripheral blood stem cells (PBSC, n = 5) using...

journal_title：Stem cells and development

authors： Rossi B,Zanolin E,Vincenzi C,Diani F,Pizzolo G,de Wynter E,Nadali G

更新日期：2004-08-01 00:00:00

abstract：:Inducing a stable and predictable program of neural cell fate in pluripotent cells in vitro is an important goal for utilizing these cells for modeling human disease mechanisms. However, the extent to which in vitro neural specification recapitulates in vivo neural specification remains to be fully established. We pre...

journal_title：Stem cells and development

authors： Dang LT,Wong L,Tropepe V

更新日期：2012-10-10 00:00:00

abstract：:Mesenchymal stems cells (MSCs) are a population of multipotent cells residing in several readily available adult tissue compartments, thus allowing for their ex vivo expansion. To date, therapeutic applications of MSCs have focused on their ability to hone to and support the repair of damaged tissues. More recent evid...

journal_title：Stem cells and development

authors： Reddy BY,Xu DS,Hantash BM

更新日期：2012-02-10 00:00:00

abstract：:Müller glia are responsible for the retina regeneration observed in zebrafish. Although the human retina harbors Müller glia with stem cell characteristics, there is no evidence that they regenerate the retina after disease or injury. Transforming growth factor-β (TGFβ) and Wnt signaling regulate retinal neurogenesis ...

journal_title：Stem cells and development

authors： Angbohang A,Wu N,Charalambous T,Eastlake K,Lei Y,Kim YS,Sun XH,Limb GA

更新日期：2016-01-01 00:00:00

abstract：:Pluripotent human embryonic stem cells (hESCs) provide appropriate systems for developmental studies and prospective donor cell sources for regenerative medicine. Identification of surface markers specific to hESCs is a prerequisite for studying hESC biology and can be used to generate clinical-level donor cell prepar...

journal_title：Stem cells and development

authors： Choi HS,Kim WT,Kim H,Kim JJ,Ko JY,Lee SW,Jang YJ,Kim SJ,Lee MJ,Jung HS,Kzhyshkowska J,Um SJ,Lee MY,Lee SH,Kim CH,Ryu CJ

更新日期：2011-04-01 00:00:00

abstract：:Mesenchymal stem cells (MSCs) have attracted much attention as potential platforms for transgene delivery and cell-based therapy for human disease. MSCs have the capability to self-renew and retain multipotency after extensive expansion in vitro, making them attractive targets for ex vivo modification and autologous t...

journal_title：Stem cells and development

authors： Ricks DM,Kutner R,Zhang XY,Welsh DA,Reiser J

更新日期：2008-06-01 00:00:00

abstract：:Occupational asthma (OA) is characterized by allergic airway inflammation and hyperresponsiveness, leading to progressive airway remodeling and a concomitant decline in lung function. The management of OA remains suboptimal in clinical practice. Thus, establishing effective therapies might overcome the natural history...

journal_title：Stem cells and development

authors： Martínez-González I,Cruz MJ,Moreno R,Morell F,Muñoz X,Aran JM

更新日期：2014-10-01 00:00:00

abstract：:Fluorescence in situ hybridization (FISH) was used to analyze peripheral blood stem cells (PBSC) and stem cell assays (SCA) derived from them in 2 patients with acute myeloid leukemia (AML) with trisomy 8 as the sole chromosome abnormality prior to undergoing autologous stem cell transplantation. In both cases, the de...

journal_title：Stem cells and development

authors： Dodge W,Cruz J,Zamkoff K,Hurd D,Pettenati MJ

更新日期：2004-02-01 00:00:00

abstract：:In this study we examine whether a somatic cell, once returned to a pluripotent state, gains the ability to reprogram other somatic cells. We reprogrammed mouse embryonic fibroblasts by viral induction of oct4, sox2, c-myc, and klf-4 genes. Upon fusion of the resulting iPS cells with somatic cells harboring an Oct4-GF...

journal_title：Stem cells and development

authors： Sumer H,Jones KL,Liu J,Heffernan C,Tat PA,Upton KR,Verma PJ

更新日期：2010-02-01 00:00:00

abstract：:Cell therapy with adult mesenchymal stem cells (MSCs) is a promising approach to regenerative medicine and autoimmune diseases. There are various approaches to improve the efficacy of MSC-based therapeutics, and MSC preparation as spheroidal aggregates, or MSC spheroids, is a novel preparatory and delivery method. Sph...

journal_title：Stem cells and development

authors： Cesarz Z,Funnell JL,Guan J,Tamama K

更新日期：2016-04-15 00:00:00

abstract：:Acute respiratory distress syndrome (ARDS)/acute lung injury (ALI) is a severe clinical respiratory failure disorder associated with chronic pathology and disability and has a mortality rate of 40%-60%. However, the pathogenesis of ARDS/ALI remains unclear, and existing therapeutic options are insufficient for address...

journal_title：Stem cells and development

authors： Wang Y,Li H,Li X,Su X,Xiao H,Yang J

更新日期：2021-02-01 00:00:00