Abstract：
:My concept of cell differentiation involves genetic information from DNA being transcribed into mRNA proteins-morphogenes (mRNAs+ homeodomain proteins)-and stored in the ovoplasm as maternal inheritance, or cytoplasmic genetic memory. Feedback mechanism(s) allow these morphogenes to selectively unlock new genes, regulating the development of the embryo. The blastomeres and the embryonic pluripotent cells of the inner cell mass of early (5 day) blastocysts are loaded with morphogenes which hamper the production of cell lines and are responsible for the formation of embryoid bodies in vitro and teratomas in vivo. There are therefore legitimate concerns as to proposals to use embryonic pluripotent cells for cell therapy and regenerative medicine. An alternative cell therapy would involve the production of tailored growth-related genes-morphogenes-and hence selective in vitro differentiation of adult de-differentiated cells.
journal_name
Stem Cells Devjournal_title
Stem cells and developmentauthors
Kristeva MAdoi
10.1089/scd.2007.0139subject
Has Abstractpub_date
2008-10-01 00:00:00pages
849-52issue
5eissn
1547-3287issn
1557-8534journal_volume
17pub_type
社论abstract：:In the central nervous system (CNS), generation of phenotypic diversity within the neuronal lineage is precisely regulated in a spatial and temporal fashion. Neural basic helix-loop-helix (bHLH) transcription factors are cell intrinsic factors that control commitment to neuronal lineage and play an important role in n...
journal_title：Stem cells and development
pub_type： 杂志文章
doi：10.1089/scd.2006.0115
更新日期：2007-08-01 00:00:00
abstract：:True tendon regeneration in human patients remains a vision of musculoskeletal therapies. In comparison to other mesenchymal lineages the biology of tenogenic differentiation is barely understood. Specifically, easy and efficient protocols are lacking that might enable tendon cell and tissue differentiation based on a...
journal_title：Stem cells and development
pub_type： 杂志文章
doi：10.1089/scd.2014.0124
更新日期：2014-08-15 00:00:00
abstract：:Mechanical cues exert considerable influence on the fates of stem cells and terminally differentiated chondrocytes. The elucidation of the interactions between cell fate and mechanical cues in nuclear mechanotransduction will provide new clues to modulate tissue homeostasis and regeneration. In this study, we used an ...
journal_title：Stem cells and development
pub_type： 杂志文章
doi：10.1089/scd.2012.0685
更新日期：2013-07-15 00:00:00
abstract：:Smooth muscle cell (SMC) differentiation and dedifferentiation play a critical role in the pathogenesis of cardiovascular diseases. The lack of a good and simple in vitro SMC differentiation system has hampered the progress of SMC field for years. The generation of such an in vitro system would be invaluable for explo...
journal_title：Stem cells and development
pub_type： 杂志文章
doi：10.1089/scd.2008.0179
更新日期：2009-06-01 00:00:00
abstract：:In spina bifida, the neural tube fails to close during the embryonic period. Exposure of the neural tube to the amniotic fluid during pregnancy causes additional neural damage. Intrauterine tissue engineering using a biomaterial seeded with stem cells might prevent this additional damage. For this purpose, autologous ...
journal_title：Stem cells and development
pub_type： 杂志文章
doi：10.1089/scd.2013.0334
更新日期：2014-03-01 00:00:00
abstract：:Human mesenchymal stromal cells (hMSCs) show great potential for clinical and experimental use due to their capacity to self-renew and differentiate into multiple mesenchymal lineages. However, disadvantages of primary cultures of hMSCs are the limited in vitro lifespan, and the variable properties of cells from diffe...
journal_title：Stem cells and development
pub_type： 杂志文章
doi：10.1089/scd.2013.0599
更新日期：2014-10-01 00:00:00
abstract：:Small-molecule inhibitors of glycogen synthase kinase 3β (GSK3β) have demonstrated strong anti-leukemia effects in preclinical studies. Here, we investigated the effect of GSK3β inhibitor 6-Bromoindirubin-3-oxime (BIO) previously shown to inhibit leukemia cell growth in vitro and of animal models on hematopoietic rege...
journal_title：Stem cells and development
pub_type： 杂志文章
doi：10.1089/scd.2014.0230
更新日期：2015-03-15 00:00:00
abstract：:Inducing a stable and predictable program of neural cell fate in pluripotent cells in vitro is an important goal for utilizing these cells for modeling human disease mechanisms. However, the extent to which in vitro neural specification recapitulates in vivo neural specification remains to be fully established. We pre...
journal_title：Stem cells and development
pub_type： 杂志文章
doi：10.1089/scd.2011.0593
更新日期：2012-10-10 00:00:00
abstract：:The mental retardation-associated protein, srGAP3 is highly expressed in neurogenic sites. It is thought to regulate the key aspects of neuronal development and functions. Little is known about the interaction between srGAP3 and immature neural stem cells/neural progenitor cells (NSCs/NPCs). In the current study, the ...
journal_title：Stem cells and development
pub_type： 杂志文章
doi：10.1089/scd.2012.0455
更新日期：2013-06-01 00:00:00
abstract：:Allergic asthma is an inflammatory airway disease caused by T helper type 2 (Th2)-driven immune responses. Recent studies have demonstrated that adipose-derived stromal cells (ASC) have an immunosuppressive effect on T-cell activity. This study was performed to investigate whether ASC can inhibit Th2-dependent allergi...
journal_title：Stem cells and development
pub_type： 杂志文章
doi：10.1089/scd.2009.0513
更新日期：2010-11-01 00:00:00
abstract：:Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) has been used extensively in cancer therapy. However, more than half of glioblastoma multiforme are insensitive to the apoptotic effect of TRAIL. Improvement in therapeutic modalities that enhances the efficacy of TRAIL in glioma is much sought after. In ...
journal_title：Stem cells and development
pub_type： 杂志文章
doi：10.1089/scd.2012.0529
更新日期：2013-07-01 00:00:00
abstract：:A murine stromal cell line (OP9-DL1) expressing a notch ligand, Delta-like-1, has been shown to be able to drive the differentiation of both murine and human hematopoietic progenitors into T cells in vitro. Further studies showed that hematopoietic progenitors transduced by a retroviral vector to express a human CD8 T...
journal_title：Stem cells and development
pub_type： 杂志文章
doi：10.1089/scd.2008.0021
更新日期：2009-03-01 00:00:00
abstract：:To develop biological approaches to restore vision, we developed a method of transplanting stem cell-derived retinal tissue into the subretinal space of a large-eye animal model (cat). Human embryonic stem cells (hESC) were differentiated to retinal organoids in a dish. hESC-derived retinal tissue was introduced into ...
journal_title：Stem cells and development
pub_type： 杂志文章
doi：10.1089/scd.2019.0090
更新日期：2019-09-01 00:00:00
abstract：:In this retrospective study, 209 patients with malignant disease were analyzed for levels of T-cell receptor excision circles (TRECs) for the first 24 months after allogeneic stem cell transplantation. CD3(+) cells were separated by direct antibody-coupled magnetic beads, followed by DNA extraction according to a stan...
journal_title：Stem cells and development
pub_type： 杂志文章
doi：10.1089/scd.2013.0588
更新日期：2014-07-15 00:00:00
abstract：:The increasing use of mesenchymal stromal cells (MSC) in clinical cellular therapy requires a safe and controlled production process compliant with Good Manufacturing Practice guidelines. Pooled blood group AB human serum (HS) has been used to replace fetal bovine serum (FBS), critically rated by the regulatory agenci...
journal_title：Stem cells and development
pub_type： 杂志文章
doi：10.1089/scd.2012.0051
更新日期：2013-03-01 00:00:00
abstract：:The difficulty in long-term expansion of mesenchymal stem cells (MSCs) using standard culture systems without the loss of their stem cell properties suggests that a critical feature of their microenvironment necessary for retention of stem cell properties is absent in these culture systems. We report here the reconsti...
journal_title：Stem cells and development
pub_type： 杂志文章
doi：10.1089/scd.2009.0217
更新日期：2010-07-01 00:00:00
abstract：:Embryonic stem cell (ESC) research gave rise to the possibility that stem cell therapy could be used in the treatment of incurable diseases such as neurodegenerative disorders. However, problems related to the tumorigenicity of undifferentiated ESCs must be resolved before such cells can be used in the application of ...
journal_title：Stem cells and development
pub_type： 杂志文章
doi：10.1089/scd.2009.0523
更新日期：2011-01-01 00:00:00
abstract：:Lymphedema is a medically irreversible condition for which currently conservative and surgical therapies are either ineffective or impractical. The potential use of progenitor and stem cell-based therapies has offered a paradigm that may provide alternative treatment options for lymphatic disorders. Moreover, basic re...
journal_title：Stem cells and development
pub_type： 杂志文章,评审
doi：10.1089/scd.2014.0390
更新日期：2015-02-01 00:00:00
abstract：:One of the challenges in stem cell research is to avoid transformation during cultivation. We studied high passage subventricular zone derived neural stem cells (NSCs) cultures of adult rats in the absence of growth factors epidermal growth factor (EGF) and basic fibroblast growth factor (bFGF). We termed this culture...
journal_title：Stem cells and development
pub_type： 杂志文章
doi：10.1089/scd.2009.0416
更新日期：2010-07-01 00:00:00
abstract：:We recently reported the isolation and characterization of a population of pancreatic progenitor cells (PPCs) from early trimester human fetal pancreata. The PPCs, being the forerunners of adult pancreatic cell lineages, were amenable to growth and differentiation into insulin-secreting islet-like cell clusters (ICCs)...
journal_title：Stem cells and development
pub_type： 杂志文章
doi：10.1089/scd.2008.0325
更新日期：2009-09-01 00:00:00
abstract：:Extracellular stresses influence transcription factor (TF) expression and therefore lineage identity in the peri-implantation mouse embryo and its stem cells. This potentially affects pregnancy outcome. To understand the effects of stress signaling during this critical period of pregnancy, we exposed cultured murine e...
journal_title：Stem cells and development
pub_type： 杂志文章
doi：10.1089/scd.2014.0157
更新日期：2014-12-15 00:00:00
abstract：:Regenerative medicine, a paragon of future healthcare, holds unprecedented potential in extending the reach of treatment modalities for individuals across diseases and lifespan. Emerging regenerative technologies, focused on structural repair and functional restoration, signal a radical transformation in medical and s...
journal_title：Stem cells and development
pub_type： 杂志文章
doi：10.1089/scd.2013.0448
更新日期：2013-12-01 00:00:00
abstract：:There is surprising confusion surrounding the concept of biological totipotency, both within the scientific community and in society at large. Increasingly, ethical objections to scientific research have both practical and political implications. Ethical controversy surrounding an area of research can have a chilling ...
journal_title：Stem cells and development
pub_type： 杂志文章
doi：10.1089/scd.2013.0364
更新日期：2014-04-15 00:00:00
abstract：:Cell therapy is a promising novel option for treatment of cardiovascular disease. Because the role of bone marrow-derived circulating progenitor cells (BM-CPCs) after cell therapy is less clear, we analyzed in this randomized, controlled study the influence of intracoronary autologous freshly isolated bone marrow cell...
journal_title：Stem cells and development
pub_type： 杂志文章,随机对照试验
doi：10.1089/scd.2010.0373
更新日期：2011-09-01 00:00:00
abstract：:The major reservoir of human multipotent mesenchymal stem/stromal cells (MSCs) is the bone marrow (BM) with the capability to control hematopoietic stem cell development. The regenerative potential of MSCs is associated with enhanced endogenous repair and healing mechanisms that modulate inflammatory responses. Our pr...
journal_title：Stem cells and development
pub_type： 杂志文章
doi：10.1089/scd.2014.0509
更新日期：2015-08-01 00:00:00
abstract：:Primary periodontal ligament stem cells (PDLSCs) are known to possess multidifferentiation potential and exhibit an immunophenotype similar to that described for bone-marrow-derived mesenchymal stem cells. In the present study, bromo-deoxyuridine (BrdU)-labeled ovine PDLSCs implanted into immunodeficient mice survived...
journal_title：Stem cells and development
pub_type： 杂志文章
doi：10.1089/scd.2013.0490
更新日期：2014-05-01 00:00:00
abstract：:Little is known about the effects of ionizing radiation on the earliest stages of embryonic development although it is well recognized that ionizing radiation is a natural part of our environment and further exposure may occur due to medical applications. The current study addresses this issue using D3 mouse embryonic...
journal_title：Stem cells and development
pub_type： 杂志文章
doi：10.1089/scd.2015.0260
更新日期：2016-01-15 00:00:00
abstract：:Basic helix-loop-helix (bHLH) transcription factors are pivotal regulators of cellular differentiation and development. The bHLH factor, Twist-1 has previously been found to control bone marrow stromal/stem cells (BMSC) self-renewal, life span, and differentiation, however not much is known about its mechanism of acti...
journal_title：Stem cells and development
pub_type： 杂志文章
doi：10.1089/scd.2014.0471
更新日期：2015-06-01 00:00:00
abstract：:A common observation in metabolic disorders and aging is the elevation of free fatty acids, which can form ectopic fat deposition and result in lipotoxicity. Ectopic fat deposition of skeletal muscle has been recognized as an important component of aging, frailty and sarcopenia. Previous studies have suggested that li...
journal_title：Stem cells and development
pub_type： 杂志文章
doi：10.1089/scd.2020.0168
更新日期：2021-01-11 00:00:00
abstract：:The placenta is a transient organ that develops upon the initiation of pregnancy and is essential for embryonic development and fetal survival. The rodent placenta consists of distinct lineages and includes cell types that are analogous to those that make up the human placenta. Trophoblast cells within the labyrinth l...
journal_title：Stem cells and development
pub_type： 杂志文章
doi：10.1089/scd.2014.0092
更新日期：2014-12-01 00:00:00
