Abstract:
:Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) has been used extensively in cancer therapy. However, more than half of glioblastoma multiforme are insensitive to the apoptotic effect of TRAIL. Improvement in therapeutic modalities that enhances the efficacy of TRAIL in glioma is much sought after. In this study, we combined the tumor selectivity of TRAIL and tumor-homing properties of mesenchymal stem cells (MSC) with gap junction (GJ) inhibitory effect of carbenoxolone (CBX) to target orthotopic glioma. MSC were engineered to express TRAIL (MSC-TRAIL) by incorporating the secretable trimeric form of TRAIL into a Herpes Simplex Virus (HSV) type I amplicon vector. Our results showed that combined treatment of MSC-TRAIL and CBX enhanced glioma cell death, especially in three primary human glioma isolates, of which two of those are marginally sensitive to TRAIL. CBX enhanced TRAIL-induced apoptosis through upregulation of death receptor 5, blockade of GJ intercellular communication, and downregulation of connexin 43. Dual arm therapy using TRAIL and CBX prolonged the survival of treated mice by ~27% when compared with the controls in an intracranial glioma model. The enhanced efficacy of TRAIL in combination with CBX coupled with the minimal cytotoxic nature of CBX suggested a favorable clinical usage of this treatment regimen.
journal_name
Stem Cells Devjournal_title
Stem cells and developmentauthors
Yulyana Y,Endaya BB,Ng WH,Guo CM,Hui KM,Lam PY,Ho IAdoi
10.1089/scd.2012.0529subject
Has Abstractpub_date
2013-07-01 00:00:00pages
1870-82issue
13eissn
1547-3287issn
1557-8534journal_volume
22pub_type
杂志文章abstract::The mental retardation-associated protein, srGAP3 is highly expressed in neurogenic sites. It is thought to regulate the key aspects of neuronal development and functions. Little is known about the interaction between srGAP3 and immature neural stem cells/neural progenitor cells (NSCs/NPCs). In the current study, the ...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2012.0455
更新日期:2013-06-01 00:00:00
abstract::Bone morphogenetic protein-2 (BMP-2) is a novel differentiation factor that is capable of inducing osteoblast differentiation and bone formation, making it an attractive option in treatment of bone defects, fractures, and spine fusions. Inflammation, which was a common situation during bone healing, is recognized to i...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2013.0345
更新日期:2014-02-01 00:00:00
abstract::Regenerative therapy of the salivary gland (SG) is a promising therapeutic approach for irreversible hyposalivation in patients with head and neck cancer treated by radiotherapy. However, little is known about the molecular regulators of stem/progenitor cell activity and regenerative processes in the SG. Wnt/β-catenin...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2009.0499
更新日期:2010-11-01 00:00:00
abstract::As granulocyte-colony-stimulating factor (G-CSF)-induced mobilization of hematopoietic stem/progenitor cells (HSPCs) increases human serum levels of hepatocyte growth factor (HGF), our aim was to investigate the role of HGF and its receptor, c-Met, in the mobilization of HSPC. CD34(+) cells and leukocytes were isolate...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2009.0376
更新日期:2010-08-01 00:00:00
abstract::Mesenchymal stem cells (MSCs) can be mobilized from the bone marrow and enter the circulation. Conversely, MSCs can be recruited from the circulation and into the bone marrow. For these migratory pathways, MSCs have to traverse the bone marrow endothelium, in a basal-to-apical and apical-to-basal direction, respective...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2011.0025
更新日期:2012-02-10 00:00:00
abstract::The aim of this study was to clarify the mechanisms that regulate hematopoietic cell expansion in vitro by identifying defined culture conditions. We report the results of experiments with CD34(+) cells from cord blood (CB, n = 13), bone marrow (BM, n = 4), and mobilized peripheral blood stem cells (PBSC, n = 5) using...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2004.13.362
更新日期:2004-08-01 00:00:00
abstract::Death ligands and their tumor necrosis factor receptor (TNFR) family receptors are the best-characterized and most efficient inducers of apoptotic signaling in somatic cells. In this study, we analyzed whether these prototypic activators of apoptosis are also expressed and able to be activated in human pluripotent ste...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2013.0057
更新日期:2013-11-15 00:00:00
abstract::Chronic idiopathic neutropenia (CIN) is a granulopoiesis disorder associated with an inhibitory bone marrow (BM) microenvironment consisting of activated T-lymphocytes and pro-inflammatory mediators. In this study, we investigated the possible involvement of BM mesenchymal stem cells (MSCs) in the pathophysiology of C...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2010.0425
更新日期:2011-08-01 00:00:00
abstract::Most disease-modifying therapeutic approaches in Alzheimer's disease (AD) aim to reduce the accumulation of neurotoxic amyloid-beta (Abeta) peptides as a hallmark of AD pathogenesis. Here we report the in vitro basis for a potential autologous stem cell-based strategy for widespread delivery of enzymatic activities ag...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2009.0045
更新日期:2010-05-01 00:00:00
abstract::Bone marrow mesenchymal stem cells (BMMSCs) have been used to treat a variety of autoimmune diseases in clinics. However, the therapeutic effects are largely dependent on the immunomodulatory capacity of culture-expanded BMMSCs. In the present study, we show that aspirin (acetylsalicylic acid, ASA)-treated BMMSCs have...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2014.0081
更新日期:2014-09-01 00:00:00
abstract::Induced pluripotent stem cell (iPSC) reprogramming requires sustained expression of multiple reprogramming factors for a limited period of time (10-30 days). Conventional iPSC reprogramming was achieved using lentiviral or simple retroviral vectors. Retroviral reprogramming has flaws of insertional mutagenesis, uncont...
journal_title:Stem cells and development
pub_type: 杂志文章,评审
doi:10.1089/scd.2013.0621
更新日期:2014-06-15 00:00:00
abstract::Recent studies have revealed that mesenchymal stem cells (MSCs) have a great potential in therapeutic applications. The low efficiency of MSC recruitment and homing to sites of diseased organ tissue, however, remains a major hurdle in their application for treatment of diseases. Stress is commonly associated with vari...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2014.0243
更新日期:2015-03-01 00:00:00
abstract::We determined whether cartilage could be engineered from mesenchymal progenitor cells (MPCs) normally found in amniotic fluid. Mesenchymal amniocytes were isolated from ovine amniotic fluid samples (n = 5) and had their identity confirmed by immunocytochemistry. Cells were expanded and then cultured as micromass pelle...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2006.15.245
更新日期:2006-04-01 00:00:00
abstract::Preclinical studies suggest that stem cell therapy (SCT) may improve poststroke recovery, and clinical trials investigating safety are ongoing. However, knowledge about patients' attitudes to SCT in stroke is limited. We evaluated the knowledge and attitudes to this therapeutic approach as well as possible factors inf...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2016.0343
更新日期:2017-04-15 00:00:00
abstract::Small-molecule inhibitors of glycogen synthase kinase 3β (GSK3β) have demonstrated strong anti-leukemia effects in preclinical studies. Here, we investigated the effect of GSK3β inhibitor 6-Bromoindirubin-3-oxime (BIO) previously shown to inhibit leukemia cell growth in vitro and of animal models on hematopoietic rege...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2014.0230
更新日期:2015-03-15 00:00:00
abstract::Neural stem cells (NSCs) have become promising tools for basic research and regenerative medicine. Intracerebral transplantation studies have suggested that these cells may be able to adopt neuronal phenotypes typical of their engraftment site and to establish appropriate connections in the recipient circuitries. Here...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2012.0530
更新日期:2013-02-15 00:00:00
abstract::With the recent advances in regenerative medicine, nanotechnology has created a niche for itself as a promising avenue in this field. Innumerable studies have been carried out by researchers using virus-based methodologies for the purpose of epigenetic reprogramming. Although this method is ostensibly safe, nonetheles...
journal_title:Stem cells and development
pub_type: 杂志文章,评审
doi:10.1089/scd.2014.0309
更新日期:2015-03-01 00:00:00
abstract::Postnatal pancreas is a potential source for progenitor cells to generate endocrine β-cells for treating type 1 diabetes. However, it remains unclear whether young (1-week-old) pancreas harbors multipotent progenitors capable of differentiating into duct, acinar, and endocrine cells. Laminin is an extracellular matrix...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2015.0007
更新日期:2015-09-01 00:00:00
abstract::Cell replacement therapy holds great promise for treating a wide range of human disorders. However, ensuring the predictable differentiation of transplanted stem cells, eliminating their risk of tumor formation, and generating fully functional cells after transplantation remain major challenges in regenerative medicin...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2010.0555
更新日期:2011-11-01 00:00:00
abstract::Retinoic acid (RA) is present at sites of neurogenesis in both the embryonic and adult brain. While it is widely accepted that RA signaling is involved in the regulation of neural stem cell differentiation, little is known about vitamin A utilization and biosynthesis of active retinoids in the neurogenic niches, or ab...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2012.0422
更新日期:2013-10-15 00:00:00
abstract::Herein we review recent data that support host tolerance of allogeneic adult mesenchymal stem cells (MSC). Evidence is emerging that donor MSC deploy a very powerful array of mechanisms that allow escape from host allogeneic responses. These mechanisms include limited expression of alloantigen by the stem cell and cel...
journal_title:Stem cells and development
pub_type: 杂志文章,评审
doi:10.1089/scd.2005.14.252
更新日期:2005-06-01 00:00:00
abstract::Periodontitis is an infectious disease characterized by chronic inflammation and progressive destruction of periodontal tissues. Chronic inflammatory environment may affect immunomodulatory function of periodontal ligament stem cells (PDLSCs) and promote shift toward proinflammatory phenotype contributing to propagati...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2019.0021
更新日期:2019-08-01 00:00:00
abstract::Amniotic fluid stem cells (AFSs) are interesting mesenchymal stem cells (MSCs) that are characterized by their great potential for cell proliferation and differentiation compared with other types of MSCs identified to date. However, MSCs in prolonged culture have been found to exhibit defects in genetic stability and ...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2012.0371
更新日期:2013-05-01 00:00:00
abstract::The enhanced proliferation of mesenchymal stem cells (MSCs) can be helpful for the clinical translation of cell therapy. Low-level laser irradiation (LLLI) has been demonstrated as regulating MSC proliferation. MicroRNAs (miRNAs) are involved in various pathophysiologic processes in stem cells, but the role of miRNAs ...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2011.0695
更新日期:2012-09-01 00:00:00
abstract::Red blood cell (RBC) differentiation from human induced pluripotent stem cells (hiPSCs) offers great potential for developmental studies and innovative therapies. However, ex vivo erythropoiesis from hiPSCs is currently limited by low efficiency and unphysiological conditions of common culture systems. Especially, the...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2019.0132
更新日期:2019-12-01 00:00:00
abstract::Small molecules (SM) can greatly enhance the efficiency of induced pluripotent stem (iPS) cell generation, but the mechanisms by which they act have not been fully explored. We show here that an SM cocktail (NaB, PD03259, and SB431542) significantly promotes iPS cell generation from human fibroblasts, and NaB is more ...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2012.0650
更新日期:2013-08-15 00:00:00
abstract::Fluorescence in situ hybridization (FISH) was used to analyze peripheral blood stem cells (PBSC) and stem cell assays (SCA) derived from them in 2 patients with acute myeloid leukemia (AML) with trisomy 8 as the sole chromosome abnormality prior to undergoing autologous stem cell transplantation. In both cases, the de...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/154732804773099227
更新日期:2004-02-01 00:00:00
abstract::Misexpression of the double homeodomain protein DUX4 in muscle is believed to cause facioscapulohumeral muscular dystrophy (FSHD). Although strategies are being devised to inhibit DUX4 activity in FSHD, there is little known about the normal function of this protein. Expression of DUX4 has been reported in pluripotent...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2012.0643
更新日期:2013-09-01 00:00:00
abstract::In spina bifida, the neural tube fails to close during the embryonic period. Exposure of the neural tube to the amniotic fluid during pregnancy causes additional neural damage. Intrauterine tissue engineering using a biomaterial seeded with stem cells might prevent this additional damage. For this purpose, autologous ...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2013.0334
更新日期:2014-03-01 00:00:00
abstract::Primordial germ cells (PGCs), the precursors of gametes, have regulatory mechanisms involving transcription factors and epigenetic modifications. The transcription factor NANOG is a key regulator of germ cell and embryonic stem cell characteristics. However, the epigenetic regulation of NANOG with a histone deacetylas...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2018.0059
更新日期:2018-09-01 00:00:00