Abstract:
:Human mesenchymal stromal cells (hMSCs) show great potential for clinical and experimental use due to their capacity to self-renew and differentiate into multiple mesenchymal lineages. However, disadvantages of primary cultures of hMSCs are the limited in vitro lifespan, and the variable properties of cells from different donors and over time in culture. In this article, we describe the generation of a telomerase-immortalized nontumorigenic human bone marrow-derived stromal mesenchymal cell line, and its detailed characterization after long-term culturing (up to 155 population doublings). The resulting cell line, iMSC#3, maintained a fibroblast-like phenotype comparable to early passages of primary hMSCs, and showed no major differences from hMSCs regarding surface marker expression. Furthermore, iMSC#3 had a normal karyotype, and high-resolution array comparative genomic hybridization confirmed normal copy numbers. The gene expression profiles of immortalized and primary hMSCs were also similar, whereas the corresponding DNA methylation profiles were more diverse. The cells also had proliferation characteristics comparable to primary hMSCs and maintained the capacity to differentiate into osteoblasts and adipocytes. A detailed characterization of the mRNA and microRNA transcriptomes during adipocyte differentiation also showed that the iMSC#3 recapitulates this process at the molecular level. In summary, the immortalized mesenchymal cells represent a valuable model system that can be used for studies of candidate genes and their role in differentiation or oncogenic transformation, and basic studies of mesenchymal biology.
journal_name
Stem Cells Devjournal_title
Stem cells and developmentauthors
Skårn M,Noordhuis P,Wang MY,Veuger M,Kresse SH,Egeland EV,Micci F,Namløs HM,Håkelien AM,Olafsrud SM,Lorenz S,Haraldsen G,Kvalheim G,Meza-Zepeda LA,Myklebost Odoi
10.1089/scd.2013.0599subject
Has Abstractpub_date
2014-10-01 00:00:00pages
2377-89issue
19eissn
1547-3287issn
1557-8534journal_volume
23pub_type
杂志文章abstract::Acute respiratory distress syndrome (ARDS)/acute lung injury (ALI) is a severe clinical respiratory failure disorder associated with chronic pathology and disability and has a mortality rate of 40%-60%. However, the pathogenesis of ARDS/ALI remains unclear, and existing therapeutic options are insufficient for address...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2020.0174
更新日期:2021-02-01 00:00:00
abstract::Body axes and germ layers evolve at gastrulation, and in mammals are driven by many genes; however, what orchestrates the genetic pathways during gastrulation remains elusive. Previously, we presented evidence that microRNA-17 (miRNA-17) family members, miR-17-5p, miR-20a, miR-93, and miR-106a were differentially expr...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2014.0051
更新日期:2015-02-01 00:00:00
abstract::Phenotypic guidance of embryonic stem (ES) cell fate is paramount if these cells are to be used for tissue repair and regeneration. Our objective was to compare two different cell culture feeders and their effect on proliferation, apoptosis, and differentiation of human (h) ES cells. HSF-6 hES cells were grown in Knoc...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2006.0109
更新日期:2007-06-01 00:00:00
abstract::Hematopoietic stem cells (HSCs) possess a distinct ability to perpetuate through self-renewal and to generate progeny that differentiate into mature cells of myeloid and lymphoid lineages. A better understanding of the molecular mechanisms by which HSCs replicate and differentiate from the perspective of developing ne...
journal_title:Stem cells and development
pub_type: 杂志文章,评审
doi:10.1089/scd.2006.15.755
更新日期:2006-12-01 00:00:00
abstract::Herein we review recent data that support host tolerance of allogeneic adult mesenchymal stem cells (MSC). Evidence is emerging that donor MSC deploy a very powerful array of mechanisms that allow escape from host allogeneic responses. These mechanisms include limited expression of alloantigen by the stem cell and cel...
journal_title:Stem cells and development
pub_type: 杂志文章,评审
doi:10.1089/scd.2005.14.252
更新日期:2005-06-01 00:00:00
abstract::Neural stem cells (NSCs) have become promising tools for basic research and regenerative medicine. Intracerebral transplantation studies have suggested that these cells may be able to adopt neuronal phenotypes typical of their engraftment site and to establish appropriate connections in the recipient circuitries. Here...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2012.0530
更新日期:2013-02-15 00:00:00
abstract::We compared the immunohistochemical distribution of (1) the novel chondroitin sulfate (CS) sulfation motifs 7D4, 4C3, and 3B3[-], (2) native heparan sulfate (HS) and Δ-HS "stubs" generated by heparitinase III digestion and (3) the HS-proteoglycan (PG), perlecan, in the fetal human elbow joint. Putative stem cell popul...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2016.0054
更新日期:2016-06-01 00:00:00
abstract::Betacellulin (BTC), a ligand of the epidermal growth factor receptor, has been shown to promote growth and differentiation of pancreatic β-cells and to improve glucose metabolism in experimental diabetic rodent models. Mesenchymal stem cells (MSCs) have been already proved to be multipotent. Recent work has attributed...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2009.0490
更新日期:2011-02-01 00:00:00
abstract::Over the past 20 months, reports claiming the generation of induced pluripotent stem (iPS) cells with characteristics identical to those of embryonic stem (ES) cells from nonembryonic tissue have captured great attention in both the scientific community and the general public. In the light of the continuing controvers...
journal_title:Stem cells and development
pub_type: 杂志文章,评审
doi:10.1089/scd.2008.0062
更新日期:2008-06-01 00:00:00
abstract::Regenerative therapy of the salivary gland (SG) is a promising therapeutic approach for irreversible hyposalivation in patients with head and neck cancer treated by radiotherapy. However, little is known about the molecular regulators of stem/progenitor cell activity and regenerative processes in the SG. Wnt/β-catenin...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2009.0499
更新日期:2010-11-01 00:00:00
abstract::Diseases of the digestive tract are complex and encompass a broad spectrum of different pathogeneses (inflammatory, ischemic, neoplastic, and functional deficit). The digestive tract is not a sterile environment, and its organs are composed of tissues with different embryologic origin and different morphologic and fun...
journal_title:Stem cells and development
pub_type: 杂志文章,评审
doi:10.1089/scd.2010.0277
更新日期:2011-07-01 00:00:00
abstract::The central role of calcium influx and electrical activity in embryonic development raises important questions about the role and regulation of voltage-dependent calcium influx. Using cultured neural progenitor cell (NPC) preparations, we recorded barium currents through voltage-activated channels using the whole-cell...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2012.0234
更新日期:2013-04-15 00:00:00
abstract::Traditionally, undifferentiated pluripotent human embryonic and induced pluripotent stem cells (hESCs and hiPSCs) have been expanded as monolayer colonies in adhesion culture, both in the presence or absence of feeder cells. However, the use of pluripotent stem cells poses the need to scale-up current culture methods....
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2010.0517
更新日期:2011-11-01 00:00:00
abstract::Human perivascular progenitor cells, including pericytes, are well-described multipotent mesenchymal cells giving rise to mesenchymal stem cells in culture. Despite the unique location of pericytes, specific antigens to distinguish human pericytes from other cell types are few. Here, we employed a human tissue microar...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2019.0106
更新日期:2019-09-15 00:00:00
abstract::In this retrospective study, 209 patients with malignant disease were analyzed for levels of T-cell receptor excision circles (TRECs) for the first 24 months after allogeneic stem cell transplantation. CD3(+) cells were separated by direct antibody-coupled magnetic beads, followed by DNA extraction according to a stan...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2013.0588
更新日期:2014-07-15 00:00:00
abstract::Ochratoxin A (OTA) is a ubiquitous fungal metabolite with nephrotoxic, carcinogenic, and apoptotic potential. Although the toxic effects of OTA in various cell types are well characterized, it is not known whether OTA has an effect on stem cell differentiation. In this study, we demonstrate that OTA inhibits adipogene...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2010.0071
更新日期:2011-03-01 00:00:00
abstract::Placental abnormalities can cause Pregnancy-Associated Disorders, including preeclampsia, intrauterine growth restriction, and placental insufficiency, resulting in complications for both the mother and fetus. Trophoblast cells within the labyrinthine layer of the placenta facilitate the exchange of nutrients, gases, ...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2016.0252
更新日期:2017-06-01 00:00:00
abstract::Mesenchymal stems cells (MSCs) are a population of multipotent cells residing in several readily available adult tissue compartments, thus allowing for their ex vivo expansion. To date, therapeutic applications of MSCs have focused on their ability to hone to and support the repair of damaged tissues. More recent evid...
journal_title:Stem cells and development
pub_type: 杂志文章,评审
doi:10.1089/scd.2011.0404
更新日期:2012-02-10 00:00:00
abstract::Stem cell therapies show great medical promise, but few new products have made it into the marketplace. The translation of stem and other cell therapies faces not only challenges associated with research and development, but also the challenges of investment funding and regulatory approval. Regulators and investors al...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2013.0188
更新日期:2013-09-15 00:00:00
abstract::Embryonic stem-like (ES-like) cells have now been derived from the inner cell mass (ICM) of horse embryos at the blastocyst stage. Because they have been shown to express cell-surface antigens found in both human and mouse ES cells, the present study investigated gene expression patterns in day-7 horse blastocysts fro...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2007.0032
更新日期:2007-10-01 00:00:00
abstract::Adipose-derived stem cells (ADSCs) are a type of multipotent mesenchymal stem cells with immunosuppressive capacities. However, the underlying mechanisms involved in the inhibitory effects of ADSCs on T cells are not completely elucidated. In this study, human peripheral blood mononuclear cells (PBMCs) stimulated with...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2018.0033
更新日期:2018-09-01 00:00:00
abstract::Bone marrow mesenchymal stem cells (MSCs) have shown potential to improve treatment of renal failure. The prohealing functions of MSCs have been found to be enhanced by treatment with the lipid mediator, 14S,21R-dihydroxy-docosa4Z,7Z,10Z,12E,16Z,19Z-hexaenoic acid (14S,21R-diHDHA). In this article, using a murine mode...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2011.0220
更新日期:2012-05-01 00:00:00
abstract::Aneuploidy is commonly seen in human preimplantation embryos, most particularly at the cleavage stage because of genome activation by third cell division. Aneuploid embryos have been used for the derivation of normal embryonic stem cell (ESC) lines and developmental modeling. This review addresses aneuploidies in huma...
journal_title:Stem cells and development
pub_type: 杂志文章,评审
doi:10.1089/scd.2013.0053
更新日期:2013-09-01 00:00:00
abstract::Progenitor expansion during development is a highly regulated process dictating the final organ size, while expansion of specific progenitor populators can adjust the final cellular composition of the organ. Understanding factors involved in these pathways is required to develop cell-based therapies such as β-cell tra...
journal_title:Stem cells and development
pub_type: 杂志文章,评审
doi:10.1089/scd.2013.0106
更新日期:2013-11-15 00:00:00
abstract::Small-molecule inhibitors of glycogen synthase kinase 3β (GSK3β) have demonstrated strong anti-leukemia effects in preclinical studies. Here, we investigated the effect of GSK3β inhibitor 6-Bromoindirubin-3-oxime (BIO) previously shown to inhibit leukemia cell growth in vitro and of animal models on hematopoietic rege...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2014.0230
更新日期:2015-03-15 00:00:00
abstract::True tendon regeneration in human patients remains a vision of musculoskeletal therapies. In comparison to other mesenchymal lineages the biology of tenogenic differentiation is barely understood. Specifically, easy and efficient protocols are lacking that might enable tendon cell and tissue differentiation based on a...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2014.0124
更新日期:2014-08-15 00:00:00
abstract::Spiral ganglion neurons (SGNs) are usually damaged in sensorineural hearing loss. SGN-derived neural stem cells (NSCs) have been identified and proposed to differentiate into neurons to replace damaged SGNs. However, it remains obscure whether SGN-NSC-derived neurons (ScNs) are electrophysiologically functional and po...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2015.0345
更新日期:2016-05-15 00:00:00
abstract::There is surprising confusion surrounding the concept of biological totipotency, both within the scientific community and in society at large. Increasingly, ethical objections to scientific research have both practical and political implications. Ethical controversy surrounding an area of research can have a chilling ...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2013.0364
更新日期:2014-04-15 00:00:00
abstract::Previous studies have transplanted a variety of neural stem cells (NSCs) to the eye in hopes of developing a therapy to replace retinal neurons lost to disease. Successful integration, survival, and differentiation of the cell types has been variably successful. At the moment, little is known about the fundamental bio...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2007.0051
更新日期:2008-02-01 00:00:00
abstract::In recent years it has been hypothesized that cancer stem cells (CSCs) are the actual driving force of tumor formation, highlighting the need to specifically target CSCs to successfully eradicate cancer growth and recurrence. Particularly, the deregulation of physiological signaling pathways controlling stem cell prol...
journal_title:Stem cells and development
pub_type: 杂志文章,评审
doi:10.1089/scd.2015.0081
更新日期:2015-10-15 00:00:00