Ionizing Radiation Impacts on Cardiac Differentiation of Mouse Embryonic Stem Cells.


:Little is known about the effects of ionizing radiation on the earliest stages of embryonic development although it is well recognized that ionizing radiation is a natural part of our environment and further exposure may occur due to medical applications. The current study addresses this issue using D3 mouse embryonic stem cells as a model system. Cells were irradiated with either X-rays or carbon ions representing sparsely and densely ionizing radiation and their effect on the differentiation of D3 cells into spontaneously contracting cardiomyocytes through embryoid body (EB) formation was measured. This study is the first to demonstrate that ionizing radiation impairs the formation of beating cardiomyocytes with carbon ions being more detrimental than X-rays. However, after prolonged culture time, the number of beating EBs derived from carbon ion irradiated cells almost reached control levels indicating that the surviving cells are still capable of developing along the cardiac lineage although with considerable delay. Reduced EB size, failure to downregulate pluripotency markers, and impaired expression of cardiac markers were identified as the cause of compromised cardiomyocyte formation. Dysregulation of cardiac differentiation was accompanied by alterations in the expression of endodermal and ectodermal markers that were more severe after carbon ion irradiation than after exposure to X-rays. In conclusion, our data show that carbon ion irradiation profoundly affects differentiation and thus may pose a higher risk to the early embryo than X-rays.


Stem Cells Dev


Helm A,Arrizabalaga O,Pignalosa D,Schroeder IS,Durante M,Ritter S




Has Abstract


2016-01-15 00:00:00












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    abstract::Postnatal pancreas is a potential source for progenitor cells to generate endocrine β-cells for treating type 1 diabetes. However, it remains unclear whether young (1-week-old) pancreas harbors multipotent progenitors capable of differentiating into duct, acinar, and endocrine cells. Laminin is an extracellular matrix...

    journal_title:Stem cells and development

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    authors: Ghazalli N,Mahdavi A,Feng T,Jin L,Kozlowski MT,Hsu J,Riggs AD,Tirrell DA,Ku HT

    更新日期:2015-09-01 00:00:00

  • Stem cell fate specification: role of master regulatory switch transcription factor PU.1 in differential hematopoiesis.

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    pub_type: 杂志文章,评审


    authors: Gangenahalli GU,Gupta P,Saluja D,Verma YK,Kishore V,Chandra R,Sharma RK,Ravindranath T

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    journal_title:Stem cells and development

    pub_type: 杂志文章,评审


    authors: Sharma S,Gurudutta GU,Satija NK,Pati S,Afrin F,Gupta P,Verma YK,Singh VK,Tripathi RP

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    journal_title:Stem cells and development

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    authors: Li M,Yu Y,Shi Y,Zhou Y,Zhang W,Hua H,Ge J,Zhang Z,Ye D,Yang C,Wang S

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  • Dental Follicle Stem Cells: Tissue Engineering and Immunomodulation.

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    authors: Zhang J,Ding H,Liu X,Sheng Y,Liu X,Jiang C

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    journal_title:Stem cells and development

    pub_type: 杂志文章


    authors: Stevenson KS,McGlynn L,Hodge M,McLinden H,George WD,Davies RW,Shiels PG

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    journal_title:Stem cells and development

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    authors: Phermthai T,Suksompong S,Tirawanchai N,Issaragrisil S,Julavijitphong S,Wichitwiengrat S,Silpsorn D,Pokathikorn P

    更新日期:2013-05-01 00:00:00

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    journal_title:Stem cells and development

    pub_type: 杂志文章


    authors: Zhong W,Tian K,Zheng X,Li L,Zhang W,Wang S,Qin J

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    journal_title:Stem cells and development

    pub_type: 杂志文章,评审


    authors: Persano L,Zagoura D,Louisse J,Pistollato F

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    authors: Huang RL,Yuan Y,Zou GM,Liu G,Tu J,Li Q

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    authors: Ricks DM,Kutner R,Zhang XY,Welsh DA,Reiser J

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    authors: Brilli E,Reitano E,Conti L,Conforti P,Gulino R,Consalez GG,Cesana E,Smith A,Rossi F,Cattaneo E

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    pub_type: 杂志文章


    authors: Park BW,Pan B,Toms D,Huynh E,Byun JH,Lee YM,Shen W,Rho GJ,Li J

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    authors: Menicanin D,Mrozik KM,Wada N,Marino V,Shi S,Bartold PM,Gronthos S

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    authors: Zhang A,London R,Schulz FM,Giguère-Simmonds PW,Delriviere L,Chandraratana H,Hardy K,Zheng S,Olynyk JK,Yeoh G

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    journal_title:Stem cells and development

    pub_type: 杂志文章


    authors: Lepage SI,Nagy K,Sung HK,Kandel RA,Nagy A,Koch TG

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    abstract::Over the past 20 months, reports claiming the generation of induced pluripotent stem (iPS) cells with characteristics identical to those of embryonic stem (ES) cells from nonembryonic tissue have captured great attention in both the scientific community and the general public. In the light of the continuing controvers...

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    pub_type: 杂志文章


    authors: Mirzaeian L,Eftekhari-Yazdi P,Esfandiari F,Eivazkhani F,Rezazadeh Valojerdi M,Moini A,Fathi R

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    authors: Kim D,Lee V,Dorsey TB,Niklason LE,Gui L,Dai G

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    journal_title:Stem cells and development

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    authors: Jensen PL,Beck HC,Petersen J,Hreinsson J,Wånggren K,Laursen SB,Sørensen PD,Christensen ST,Andersen CY

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    journal_title:Stem cells and development

    pub_type: 杂志文章


    authors: Lai Y,Sun Y,Skinner CM,Son EL,Lu Z,Tuan RS,Jilka RL,Ling J,Chen XD

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    journal_title:Stem cells and development

    pub_type: 杂志文章


    authors: Garcia TX,Parekh P,Gandhi P,Sinha K,Hofmann MC

    更新日期:2017-04-15 00:00:00

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    journal_title:Stem cells and development

    pub_type: 杂志文章


    authors: Kao CL,Tai LK,Chiou SH,Chen YJ,Lee KH,Chou SJ,Chang YL,Chang CM,Chen SJ,Ku HH,Li HY

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    journal_title:Stem cells and development

    pub_type: 杂志文章


    authors: Heng BC,Cao T,Bested SM,Tong GQ,Ng SC

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    journal_title:Stem cells and development

    pub_type: 杂志文章


    authors: Li Q,Louden E,Zhou J,Drewlo S,Dai J,Puscheck EE,Chen K,Rappolee DA

    更新日期:2019-01-15 00:00:00

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    journal_title:Stem cells and development

    pub_type: 杂志文章


    authors: Dodge W,Cruz J,Zamkoff K,Hurd D,Pettenati MJ

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    journal_title:Stem cells and development

    pub_type: 杂志文章


    authors: Kamon M,Katano M,Hiraki-Kamon K,Hishida T,Nakachi Y,Mizuno Y,Okazaki Y,Suzuki A,Hirasaki M,Ueda A,Nishimoto M,Kato H,Okuda A

    更新日期:2014-09-15 00:00:00

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    journal_title:Stem cells and development

    pub_type: 杂志文章


    authors: Clark AR,Carter AB,Hager LE,Price EM

    更新日期:2016-08-01 00:00:00

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    journal_title:Stem cells and development

    pub_type: 杂志文章


    authors: Zhou K,Guo S,Tong S,Sun Q,Li F,Zhang X,Qiao Y,Liang G

    更新日期:2018-09-01 00:00:00