Ionizing Radiation Impacts on Cardiac Differentiation of Mouse Embryonic Stem Cells.


:Little is known about the effects of ionizing radiation on the earliest stages of embryonic development although it is well recognized that ionizing radiation is a natural part of our environment and further exposure may occur due to medical applications. The current study addresses this issue using D3 mouse embryonic stem cells as a model system. Cells were irradiated with either X-rays or carbon ions representing sparsely and densely ionizing radiation and their effect on the differentiation of D3 cells into spontaneously contracting cardiomyocytes through embryoid body (EB) formation was measured. This study is the first to demonstrate that ionizing radiation impairs the formation of beating cardiomyocytes with carbon ions being more detrimental than X-rays. However, after prolonged culture time, the number of beating EBs derived from carbon ion irradiated cells almost reached control levels indicating that the surviving cells are still capable of developing along the cardiac lineage although with considerable delay. Reduced EB size, failure to downregulate pluripotency markers, and impaired expression of cardiac markers were identified as the cause of compromised cardiomyocyte formation. Dysregulation of cardiac differentiation was accompanied by alterations in the expression of endodermal and ectodermal markers that were more severe after carbon ion irradiation than after exposure to X-rays. In conclusion, our data show that carbon ion irradiation profoundly affects differentiation and thus may pose a higher risk to the early embryo than X-rays.


Stem Cells Dev


Helm A,Arrizabalaga O,Pignalosa D,Schroeder IS,Durante M,Ritter S




Has Abstract


2016-01-15 00:00:00












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    authors: Jensen PL,Beck HC,Petersen J,Hreinsson J,Wånggren K,Laursen SB,Sørensen PD,Christensen ST,Andersen CY

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    authors: Gul-Uludag H,Xu P,Marquez-Curtis LA,Xing J,Janowska-Wieczorek A,Chen J

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    pub_type: 杂志文章,评审


    authors: Abdelalim EM,Emara MM,Kolatkar PR

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    authors: Rai M,Walthall JM,Hu J,Hatzopoulos AK

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    authors: Thoma EC,Wagner TU,Weber IP,Herpin A,Fischer A,Schartl M

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    authors: Veronesi F,Giavaresi G,Tschon M,Borsari V,Nicoli Aldini N,Fini M

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    authors: Mellows B,Mitchell R,Antonioli M,Kretz O,Chambers D,Zeuner MT,Denecke B,Musante L,Ramachandra DL,Debacq-Chainiaux F,Holthofer H,Joch B,Ray S,Widera D,David AL,Huber TB,Dengjel J,De Coppi P,Patel K

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    authors: Deng Y,Yi S,Wang G,Cheng J,Zhang Y,Chen W,Tai Y,Chen S,Chen G,Liu W,Zhang Q,Yang Y

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    authors: Cesarz Z,Funnell JL,Guan J,Tamama K

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    authors: Bernecker C,Ackermann M,Lachmann N,Rohrhofer L,Zaehres H,Araúzo-Bravo MJ,van den Akker E,Schlenke P,Dorn I

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    authors: Hu K

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  • Dental Follicle Stem Cells: Tissue Engineering and Immunomodulation.

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    authors: Zhang J,Ding H,Liu X,Sheng Y,Liu X,Jiang C

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  • Mesenchymal stem cells overexpressing CXCR4 attenuate remodeling of postmyocardial infarction by releasing matrix metalloproteinase-9.

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    journal_title:Stem cells and development

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    authors: Magin AS,Körfer NR,Partenheimer H,Lange C,Zander A,Noll T

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    journal_title:Stem cells and development

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    authors: Solmesky LJ,Abekasis M,Bulvik S,Weil M

    更新日期:2009-11-01 00:00:00

  • Alteration Analysis of Bone Marrow Mesenchymal Stromal Cells from De Novo Acute Myeloid Leukemia Patients at Diagnosis.

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    authors: Desbourdes L,Javary J,Charbonnier T,Ishac N,Bourgeais J,Iltis A,Chomel JC,Turhan A,Guilloton F,Tarte K,Demattei MV,Ducrocq E,Rouleux-Bonnin F,Gyan E,Hérault O,Domenech J

    更新日期:2017-05-15 00:00:00

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    journal_title:Stem cells and development

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    authors: Garcia TX,Parekh P,Gandhi P,Sinha K,Hofmann MC

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    journal_title:Stem cells and development

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    authors: Lukovic D,Stojkovic M,Moreno-Manzano V,Bhattacharya SS,Erceg S

    更新日期:2014-01-01 00:00:00

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    journal_title:Stem cells and development

    pub_type: 杂志文章


    authors: Ratushnyy A,Ezdakova M,Yakubets D,Buravkova L

    更新日期:2018-06-15 00:00:00

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    journal_title:Stem cells and development

    pub_type: 杂志文章


    authors: Kim SY,Kim MJ,Jung H,Kim WK,Kwon SO,Son MJ,Jang IS,Choi JS,Park SG,Park BC,Han YM,Lee SC,Cho YS,Bae KH

    更新日期:2012-05-20 00:00:00

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    journal_title:Stem cells and development

    pub_type: 杂志文章


    authors: Shi Q,Hodara V,Simerly CR,Schatten GP,VandeBerg JL

    更新日期:2013-02-15 00:00:00

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    journal_title:Stem cells and development

    pub_type: 杂志文章


    authors: Yang WZ,Yu WY,Chen T,Wang XF,Dong F,Xie ME,Gong YM,Liang HY,Fu WC

    更新日期:2019-07-15 00:00:00

  • Forced Runx1 expression in human neural stem/progenitor cells transplanted to the rat dorsal root ganglion cavity results in extensive axonal growth specifically from spinal cord-derived neurospheres.

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    journal_title:Stem cells and development

    pub_type: 杂志文章


    authors: König N,Åkesson E,Telorack M,Vasylovska S,Ngamjariyawat A,Sundström E,Oster A,Trolle C,Berens C,Aldskogius H,Seiger Å,Kozlova EN

    更新日期:2011-11-01 00:00:00

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    abstract::Stromal cells have been used to induce dopaminergic differentiation of mouse, primate, and human embryonic stem cells (hESCs), but the mechanism that governs this induction is unknown. In this manuscript, we show that medium conditioned by the stromal cell line PA6 (PA6-CM) can induce dopaminergic differentiation in n...

    journal_title:Stem cells and development

    pub_type: 杂志文章


    authors: Swistowska AM,da Cruz AB,Han Y,Swistowski A,Liu Y,Shin S,Zhan M,Rao MS,Zeng X

    更新日期:2010-01-01 00:00:00