Abstract:
:Recent evidence shows that amniotic fluid (AF) contains multiple cell types derived from the developing fetus, and may represent a novel source of stem cells for cell therapy. In this study, we examined the paracrine factors released by human amniotic fluid-derived mesenchymal stem cells (AF-MSCs) and their ability to accelerate the wound-healing process by stimulating proliferation and migration of dermal fibroblasts. AF-MSCs expressed the typical MSC marker proteins CD13, CD29, and CD44 and differentiated into adipocytes, osteoblasts, and chondrocytes when exposed to the appropriate differentiation media. In addition, AF-MSC-conditioned media (AF-MSC-CM) significantly enhanced proliferation of dermal fibroblasts. Antibody-based protein array and enzyme-linked immunosorbent assay (ELISA) indicated that AF-MSC-CM contains various cytokines and chemokines that are known to be important in normal wound healing, including IL-8, IL-6, TGF-beta, TNFRI, VEGF, and EGF. Application of AF-MSC-CM significantly enhanced wound healing by dermal fibroblasts via the TGF-beta/SMAD2 pathway. Levels of p-SMAD2 were increased by AF-MSC-CM, and both the increase in p-SMAD2 and migration of dermal fibroblasts were blocked by inhibiting the TGF-beta/SMAD2 pathway. Moreover, in a mouse excisional wound model, AF-MSC-CM accelerated wound healing. These data provide the first evidence of the potential for AF-MSC-CM in the treatment of skin wounds.
journal_name
Stem Cells Devjournal_title
Stem cells and developmentauthors
Yoon BS,Moon JH,Jun EK,Kim J,Maeng I,Kim JS,Lee JH,Baik CS,Kim A,Cho KS,Lee JH,Lee HH,Whang KY,You Sdoi
10.1089/scd.2009.0138subject
Has Abstractpub_date
2010-06-01 00:00:00pages
887-902issue
6eissn
1547-3287issn
1557-8534journal_volume
19pub_type
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journal_title:Stem cells and development
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journal_title:Stem cells and development
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journal_title:Stem cells and development
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journal_title:Stem cells and development
pub_type: 杂志文章
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journal_title:Stem cells and development
pub_type: 杂志文章
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journal_title:Stem cells and development
pub_type: 杂志文章
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更新日期:2014-12-01 00:00:00
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journal_title:Stem cells and development
pub_type: 杂志文章
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journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2018.0055
更新日期:2018-08-15 00:00:00
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journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2011.0220
更新日期:2012-05-01 00:00:00
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journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2020.0117
更新日期:2020-12-01 00:00:00
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journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2009.0217
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journal_title:Stem cells and development
pub_type: 杂志文章
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journal_title:Stem cells and development
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journal_title:Stem cells and development
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journal_title:Stem cells and development
pub_type: 杂志文章
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journal_title:Stem cells and development
pub_type: 杂志文章
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journal_title:Stem cells and development
pub_type: 杂志文章
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更新日期:2012-04-10 00:00:00
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journal_title:Stem cells and development
pub_type: 杂志文章
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更新日期:2019-05-01 00:00:00
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journal_title:Stem cells and development
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更新日期:2014-04-15 00:00:00
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journal_title:Stem cells and development
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更新日期:2014-01-15 00:00:00
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journal_title:Stem cells and development
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更新日期:2014-10-01 00:00:00
abstract::Mesenchymal stems cells (MSCs) are a population of multipotent cells residing in several readily available adult tissue compartments, thus allowing for their ex vivo expansion. To date, therapeutic applications of MSCs have focused on their ability to hone to and support the repair of damaged tissues. More recent evid...
journal_title:Stem cells and development
pub_type: 杂志文章,评审
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更新日期:2012-02-10 00:00:00