Abstract:
:P311 is a newly discovered functional gene, and it has been proved to play a key role in blood pressure homeostasis, glioblastoma invasion, renal fibrosis, hypertrophic scar formation, and others. In this study, for the first time, we found that P311 could enhance reepithelialization during wound healing via promoting epidermal stem cell (EpSC) migration through Rho GTPases. P311 expression was highly increased in neo-epidermal cells during human and mouse skin wound healing, and P311was co-localized with 5-bromo-2'-deoxyuridine positive label-retaining cells in a mouse superficial second-degree burn wound model. Furthermore, transfection of human EpSCs with adenovirus encoding P311 significantly accelerated the cell migration in vitro. Moreover, highly expressed P311 could enhance the activities of the Rho GTPases (RhoA, Rac1, and Cdc42) in cultured human EpSCs. P311-knockout mouse EpSCs showed dramatically decreased cell migration and activities of Rho GTPases (RhoA, Rac1, and Cdc42). Besides, both the RhoA-specific inhibitor and the Rac1 inhibitor, not the Cdc42 inhibitor, could significantly suppress P311-induced human EpSC migration. In vivo, the reepithelialization was markedly impaired during wound healing after P311 was knocked out. Together, our results suggested that P311 could accelerate skin wound reepithelialization by promoting the migration of EpSCs through RhoA and Rac1 activation. P311 could serve as a novel target for regulation of EpSC migration during cutaneous wound healing.
journal_name
Stem Cells Devjournal_title
Stem cells and developmentauthors
Yao Z,Li H,He W,Yang S,Zhang X,Zhan R,Xu R,Tan J,Zhou J,Wu J,Luo Gdoi
10.1089/scd.2016.0249subject
Has Abstractpub_date
2017-03-15 00:00:00pages
451-460issue
6eissn
1547-3287issn
1557-8534journal_volume
26pub_type
杂志文章abstract::Mechanical cues exert considerable influence on the fates of stem cells and terminally differentiated chondrocytes. The elucidation of the interactions between cell fate and mechanical cues in nuclear mechanotransduction will provide new clues to modulate tissue homeostasis and regeneration. In this study, we used an ...
journal_title:Stem cells and development
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abstract::Human stem cells from various sources have potential therapeutic applications. The clinical implementation of these therapies introduces the need for methods of noninvasive tracking of cells. The purpose of this study was to evaluate a high resolution magnetic resonance imaging (MRI) technique for in vivo detection an...
journal_title:Stem cells and development
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journal_title:Stem cells and development
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journal_title:Stem cells and development
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abstract::Myocardial infarction (MI) results in loss of myofibers in the ischemic zone of the heart, followed by scar formation. These factors increase barriers to mobilization of mesenchymal stem cells (MSC), thereby impeding their effectiveness in cardiac repair. This study examined MSC overexpressing CXCR4 (MSC(CX4)) to dete...
journal_title:Stem cells and development
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abstract::Stem cell therapy is a promising therapeutic strategy for treating myocardial infarction (MI). However, it is necessary to identify ideal adult stem cells for transplantation and explore mechanisms of the transplanted cells in improving cardiac functions after MI. In this study, a population of embryonic-like stem cel...
journal_title:Stem cells and development
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journal_title:Stem cells and development
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journal_title:Stem cells and development
pub_type: 杂志文章,评审
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abstract::Induced pluripotent stem cells (iPSCs) are somatic cells that have been reprogrammed to a pluripotent state via introduction of defined transcription factors. iPSCs are a valuable resource for regenerative medicine, but whether iPSCs are identical to embryonic stem cells (ESCs) remains unclear. In this study, we perfo...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2011.0243
更新日期:2012-05-20 00:00:00
abstract::Embryonic stem cells (ESCs) have the capacity to form all the tissues in the body and hence, directed differentiation of ESCs along specific lineages represents a means to generate therapeutically useful cell types. It has been postulated that, during in vitro differentiation, ES cells sequentially pass through simila...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2005.14.656
更新日期:2005-12-01 00:00:00
abstract::Transplantation of neural stem/progenitor cells (NSPCs) is a promising strategy for repair of the diseased/injured central nervous system (CNS); however, controlling their differentiation remains a significant hurdle. This study is aimed at controlling differentiation and specifically at screening exogenous factors to...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2008.0412
更新日期:2009-12-01 00:00:00
abstract::The activation and transcriptional activity of signal transducer and activator of transcription 3 (STAT3) is essential for maintaining mouse embryonic stem (ES) cell cultures in an undifferentiated state. However, reports from human and monkey ES-cell culture suggest that STAT3 is dispensable for pluripotency in these...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2007.0262
更新日期:2009-01-01 00:00:00
abstract::Small-molecule inhibitors of glycogen synthase kinase 3β (GSK3β) have demonstrated strong anti-leukemia effects in preclinical studies. Here, we investigated the effect of GSK3β inhibitor 6-Bromoindirubin-3-oxime (BIO) previously shown to inhibit leukemia cell growth in vitro and of animal models on hematopoietic rege...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2014.0230
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journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2008.0179
更新日期:2009-06-01 00:00:00
abstract::Human mesenchymal stromal cells (hMSCs) show great potential for clinical and experimental use due to their capacity to self-renew and differentiate into multiple mesenchymal lineages. However, disadvantages of primary cultures of hMSCs are the limited in vitro lifespan, and the variable properties of cells from diffe...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2013.0599
更新日期:2014-10-01 00:00:00
abstract::Differential staining is an immunocytochemical staining that visualizes trophectoderm (TE) and the inner cell mass (ICM) of the blastocysts. It is used to determine the blastocyst quality, but could also be a useful tool to assess the integration site of injected cells into the early embryo. This is relevant for testi...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2014.0503
更新日期:2015-05-01 00:00:00
abstract::Adipose-derived stem cells (ADSCs) are a type of multipotent mesenchymal stem cells with immunosuppressive capacities. However, the underlying mechanisms involved in the inhibitory effects of ADSCs on T cells are not completely elucidated. In this study, human peripheral blood mononuclear cells (PBMCs) stimulated with...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2018.0033
更新日期:2018-09-01 00:00:00
abstract::Osteogenesis imperfecta (OI) is a genetic bone pathology with prenatal onset, characterized by brittle bones in response to abnormal collagen composition. There is presently no cure for OI. We previously showed that human first trimester fetal blood mesenchymal stem cells (MSCs) transplanted into a murine OI model (oi...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2013.0132
更新日期:2014-02-01 00:00:00
abstract::Bone marrow mesenchymal stem cells (MSCs) have shown potential to improve treatment of renal failure. The prohealing functions of MSCs have been found to be enhanced by treatment with the lipid mediator, 14S,21R-dihydroxy-docosa4Z,7Z,10Z,12E,16Z,19Z-hexaenoic acid (14S,21R-diHDHA). In this article, using a murine mode...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2011.0220
更新日期:2012-05-01 00:00:00
abstract::Esophagus and trachea arise from a common origin, the anterior foregut tube. The compartmentalization process of the foregut into the esophagus and trachea is still poorly understood. Esophageal atresia/tracheoesophageal fistula (EA/TEF) is one of the most common gastrointestinal congenital defects with an incidence r...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2020.0075
更新日期:2020-08-01 00:00:00
abstract::Traditionally, undifferentiated pluripotent human embryonic and induced pluripotent stem cells (hESCs and hiPSCs) have been expanded as monolayer colonies in adhesion culture, both in the presence or absence of feeder cells. However, the use of pluripotent stem cells poses the need to scale-up current culture methods....
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2010.0517
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abstract::Nonpluripotent neural progenitor cells (NPCs) derived from the human fetal central nervous system were found to express a number of messenger RNA (mRNA) species associated with pluripotency, such as NANOG, REX1, and OCT4. The expression was restricted to small subpopulations of NPCs. In contrast to pluripotent stem ce...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2016.0346
更新日期:2017-06-15 00:00:00
abstract::Bone marrow human mesenchymal stem cells (hMSCs) are known to survive in serum-free media, when most normal somatic cells do not survive. We found that the endogenously-activated bone morphogenetic protein (BMP) pathway is involved in this cellular behavior. Under this culture condition, phosphorylated Smad1 (PSmad1),...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2009.0020
更新日期:2009-11-01 00:00:00
abstract::Misexpression of stem cell-related genes may occur in some cancer cells, influencing patient's prognosis. This is the case of medulloblastoma, a common and clinically challenging malignant tumor of the central nervous system, where expression of the pluripotency factor, OCT4, is correlated with poor survival. A downst...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2015.0052
更新日期:2015-11-15 00:00:00
abstract::We determined whether cartilage could be engineered from mesenchymal progenitor cells (MPCs) normally found in amniotic fluid. Mesenchymal amniocytes were isolated from ovine amniotic fluid samples (n = 5) and had their identity confirmed by immunocytochemistry. Cells were expanded and then cultured as micromass pelle...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2006.15.245
更新日期:2006-04-01 00:00:00
abstract::Bronchopulmonary dysplasia (BPD), the main complication of extreme prematurity, has lifelong consequences for lung health. Mesenchymal stromal cells (MSCs) prevent lung injury in experimental BPD in newborn rodents when given in the immediate neonatal period. Whether MSC therapy can restore normal lung growth after es...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2019.0116
更新日期:2020-03-15 00:00:00
abstract::Rare heterozygous deletions in the neurexin 1 (NRXN1) gene robustly increase an individual's risk of developing neurological and psychiatric disorders. However, the molecular bases by which different mutations result in different clinical presentations, with variable penetrance, are unknown. To better understand the m...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2020.0017
更新日期:2020-09-01 00:00:00
abstract::The success rate of human embryonic stem cell (hESC) derivation depends on both culture conditions and embryo quality and is routinely determined by morphological criteria. However, high incidence of chromosomal abnormality even in high-grade cleavage embryos from in vitro fertilization (IVF) patients suggests that th...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2009.0136
更新日期:2010-01-01 00:00:00
abstract::Vascular smooth muscle cells (SMCs), which arise from multiple embryonic progenitors, have unique lineage-specific properties and this diversity may contribute to spatial patterns of vascular diseases. We developed in vitro methods to generate distinct vascular SMC subtypes from human pluripotent stem cells, allowing ...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2014.0520
更新日期:2015-04-01 00:00:00
abstract::The aim of stem cell therapy after cardiac injury is to replace damaged cardiac tissue. Human cardiac progenitor cells (CPCs) represent an interesting cell population for clinical strategies to treat cardiac disease and human CPC-specific antibodies would aid in the clinical implementation of cardiac progenitor-based ...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2017.0048
更新日期:2017-11-01 00:00:00